Macromolecular Glucosamine-Containing Component of the Surface of Cultivated Mouse Cells

1970 ◽  
Vol 7 (2) ◽  
pp. 337-355
Author(s):  
K. ONODERA ◽  
ROSE SHEININ
Keyword(s):  
Plasma Membrane ◽  
Cell Division ◽  
Cell Surface ◽  
Plating Efficiency ◽  
Transformed Cells ◽  
3T3 Cells ◽  
Vital Stain ◽  
Surface Component ◽  
Mouse Cells ◽  
Macromolecular Component

It has been demonstrated that a glucosamine-containing macromolecular component of the cell surface of 3T3 mouse cells, and SV40-transformed cells, is released from cells by treatment with trypsin under conditions in which the plasma membrane remains functionally intact. This was shown by the fact that the treated cells could be cloned with high plating efficiency and remained impermeable to the vital stain, erythrocin. A method for specifically marking this surface component has been devised based on the finding that in 3T3 cells growing synchronously after subculture by trypsin maximum incorporation of glucosamine into this material occurs 12-13 h thereafter. Of the total radioactive glucosamine incorporated into macro-molecular cell constituents, over 80% was recovered in surface component. Studies on the biosynthesis of surface component revealed that this was periodic during a cycle of cell duplication, with an increased rate of formation immediately after cell division. It was found that the surface component of 3T3 cells differed from that of SV40-transformed cells.

scholarly journals Glycoproteins of coated pits, cell junctions, and the entire cell surface revealed by monoclonal antibodies and immunoelectron microscopy.

1983 ◽  
Vol 97 (2) ◽  
pp. 533-541 ◽  
Author(s):  
T L Murphy ◽  
G Decker ◽  
J T August
Keyword(s):  
Monoclonal Antibodies ◽  
Plasma Membrane ◽  
Cell Surface ◽  
Immunoelectron Microscopy ◽  
Total Cell ◽  
Cell Junctions ◽  
Membrane Glycoproteins ◽  
3T3 Cells ◽  
Coated Pits ◽  
Entire Cell

Topographical descriptions of three major plasma membrane glycoproteins of murine 3T3 cells were obtained by immunoelectron microscopy with monoclonal antibodies. A glycoprotein of Mr 80,000 was distributed throughout the total cell surface. A second of Mr 90,000 was concentrated in coated pits, and a third of Mr 100,000 was localized at cell junctions.

scholarly journals DIBUTYRYL CYCLIC ADENOSINE 3' 5' MONOPHOSPHATE, SUGAR TRANSPORT, AND REGULATORY CONTROL OF CELL DIVISION IN NORMAL AND TRANSFORMED CELLS

1973 ◽  
Vol 56 (2) ◽  
pp. 487-491 ◽  
Author(s):  
William J. Grimes ◽  
Judith L. Schroeder
Keyword(s):  
Cell Division ◽  
Cell Growth ◽  
Sugar Transport ◽  
Cell Number ◽  
High Density ◽  
Regulatory Control ◽  
Transformed Cells ◽  
3T3 Cells ◽  
Lower Ability ◽  
Swiss 3T3

Swiss 3T3 cells exhibit contact-regulated cell growth and have a lower ability to transport 2-deoxyglucose than polyoma (Py)-transformed 3T3 cells. Py3T3 cells treated with dibutyryl cyclic adenosine 3'5' monophosphate (dBcAMP) and theophylline have reduced cell growth and transport 2-deoxyglucose at the same rate as normal 3T3 cells. Evidence that the cessation of cell growth and reduced transport abilities in Py3T3 cells does not represent a return to contact-regulated growth comes from the following observations. First, treating high density Py3T3 cells with dBcAMP allows more than two doublings of cell number, even though ability to transport 2-deoxyglucose is returned to levels equal to those of normal 3T3 cells. Second, dBcAMP prevents serum-stimulated increases in 2-deoxyglucose transport in Py3T3 but not in 3T3 cells.

The cytological effects of myxin on Escherichia coli

1969 ◽  
Vol 15 (7) ◽  
pp. 707-711 ◽  
Author(s):  
I. L. Stevenson
Keyword(s):  
Escherichia Coli ◽  
Plasma Membrane ◽  
Cell Division ◽  
Cell Surface ◽  
Microscopic Examination ◽  
Cell Elongation ◽  
Nuclear Bodies ◽  
Abnormal Development ◽  
Macromolecular Synthesis ◽  
Apparent Effect

When actively growing cells of Escherichia coli 15T− were treated with the new antibiotic myxin, the effects produced by the antibiotic varied, depending on the concentration. With a concentration of 1 μg/ml, the action was bacteriostatic for the first 60 min exposure. Cell division was inhibited but there was no apparent effect on macromolecular synthesis as evidenced by the formation of filamentous cells. At higher concentrations (3 and 5 μg/ml), myxin was bactericidal although some cell elongation occurred at the 3 μg/ml level. Microscopic examination of cells treated with 3 and 5 μg/ml for 30–45 min indicated the presence of vacuolated areas which thin section studies revealed as intracellular ramifications of the plasma membrane. Protoplasts of cells grown in the presence of 5 μg/ml myxin were enlarged or rapidly lysed even after as little as 15 min exposure. Myxin has no effect on the cell surface of control protoplasts. No abnormal development of the plasma membrane was noted in the elongated cells formed in the presence of 1 μg/ml myxin. Except for the lack of septation during the first 60 min, the filamentous forms appeared normal with well-defined nuclear bodies distributed throughout the cell.

Studies on the biochemical properties of surface component of normal and SV-40 transformed 3T3 mouse cells

1970 ◽  
Vol 48 (8) ◽  
pp. 851-857 ◽  
Author(s):  
Rose Sheinin ◽  
Kazukiyo Onodera
Keyword(s):  
Biochemical Properties ◽  
3T3 Cells ◽  
Surface Component ◽  
Mouse Cells ◽  
Marked Surface

A specifically marked surface component from 3T3 mouse cells and from SV-40 transformed 3T3 cells has been isolated and partially purified. These components have been shown to be free of RNA, DNA, and lipid. They do contain carbohydrate and peptide, probably as glycoprotein.

Cell surface alteration in Epstein-Barr virus-transformed cells from patients with extreme insulin resistance

Diabetes ◽  
1990 ◽  
Vol 39 (8) ◽  
pp. 924-927 ◽  
Author(s):  
D. L. Gorden ◽  
A. Robert ◽  
V. Y. Moncada ◽  
S. I. Taylor ◽  
J. Muhlhauser ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Cell Surface ◽  
Epstein Barr Virus ◽  
Transformed Cells ◽  
Barr Virus ◽  
Surface Alteration ◽  
Extreme Insulin Resistance ◽  
Epstein Barr

scholarly journals Correlative AFM and fluorescence imaging demonstrate nanoscale membrane remodeling and ring-like and tubular structure formation by septins

Nanoscale ◽  
2021 ◽  
Author(s):  
Anthony Vial ◽  
Cyntia Taveneau ◽  
Luca Costa ◽  
brieuc chauvin ◽  
hussein nasrallah ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Cell Division ◽  
Structure Formation ◽  
Fluorescence Imaging ◽  
Tubular Structure ◽  
Membrane Remodeling

Septins are ubiquitous cytoskeletal filaments that interact with the inner plasma membrane and are essential for cell division in eukaryotes. In cellular contexts, septins are often localized at micrometric gaussian...

scholarly journals Endogenous hyaluronate-cell surface interactions in 3T3 and simian virus-transformed 3T3 cells.

1984 ◽  
Vol 259 (15) ◽  
pp. 9440-9446
Author(s):  
R L Goldberg ◽  
J D Seidman ◽  
G Chi-Rosso ◽  
B P Toole
Keyword(s):  
Cell Surface ◽  
Surface Interactions ◽  
Simian Virus ◽  
3T3 Cells ◽  
Cell Surface Interactions
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