A Study of Potassium Permanganate ‘Fixation’ for Electron Microscopy
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The action of buffered potassium permanganate as a fixative for electron microscopy has been investigated. Electron contrast has been shown to be produced by the deposition of granular material in the tissue. The particles are about 50 Å in diameter. Actual fixation of the tissue is performed by the dehydrating alcohol. Histochemical studies have shown that RNA and histones are removed, whereas phospholipid-protein complexes are ‘unmasked’. The reaction of the permanganate with unmasked protein gives rise to high membrane contrast in electron micrographs.
1967 ◽
Vol 25
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pp. 190-191
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1994 ◽
Vol 52
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pp. 88-89
2018 ◽
Vol 294
(5)
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pp. 1602-1608
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2012 ◽
Vol 180
(1)
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pp. 249-253
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2017 ◽
pp. 377-413
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Keyword(s):
2021 ◽
pp. 217-232
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