A Study of the Osmium Techniques for the ‘Golgi Apparatus’

1954 ◽  
Vol s3-95 (31) ◽  
pp. 383-388
Author(s):  
JOHN R. BAKER
Keyword(s):  
Epithelial Cell ◽  
Golgi Apparatus ◽  
Intestinal Epithelial Cell ◽  
Intestinal Epithelial ◽  
Black Precipitate ◽  
Golgi Region

The paper is concerned with the action of the osmium techniques for the ‘Golgi apparatus’ on the intestinal epithelial cell of the mouse. It is shown that, when the fixative has acted, there are many separate lipide spheres in the ‘Golgi’ region. During postosmication the spaces between these spheres are filled up with a black precipitate. This black precipitate does not represent the distribution of lipide material.

Tu1198 Intestinal Epithelial Cell Extrusion and Microbial Virulence Are Altered in Pediatric Inflammatory Bowel Diseases

2014 ◽  
Vol 146 (5) ◽  
pp. S-781
Author(s):  
Deenaz Zaidi ◽  
Michael Bording-Jorgenson ◽  
Hien Q. Huynh ◽  
Yuefei Lou ◽  
Julia J. Liu ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Inflammatory Bowel Diseases ◽  
Intestinal Epithelial Cell ◽  
Intestinal Epithelial ◽  
Bowel Diseases ◽  
Inflammatory Bowel ◽  
Cell Extrusion

scholarly journals Enhanced intestinal epithelial cell proliferation in diabetic rats correlates with β-catenin accumulation

2015 ◽  
Vol 226 (3) ◽  
pp. 135-143 ◽  
Author(s):  
Tatiana Dorfman ◽  
Yulia Pollak ◽  
Rima Sohotnik ◽  
Arnold G Coran ◽  
Jacob Bejar ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Epithelial Cell ◽  
Real Time ◽  
Real Time Pcr ◽  
Intestinal Epithelial Cell ◽  
Diabetic Rats ◽  
Male Rats ◽  
Intestinal Cell ◽  
Intestinal Epithelial ◽  
Epithelial Cell Proliferation

The Wnt/β-catenin signaling cascade is implicated in the control of stem cell activity, cell proliferation, and cell survival of the gastrointestinal epithelium. Recent evidence indicates that the Wnt/β-catenin pathway is activated under diabetic conditions. The purpose of this study was to evaluate the role of Wnt/β-catenin signaling during diabetes-induced enteropathy in a rat model. Male rats were divided into three groups: control rats received injections of vehicle; diabetic rats received injections of one dose of streptozotocin (STZ); and diabetic–insulin rats received injections of STZ and were treated with insulin given subcutaneously at a dose of 1 U/kg twice daily. Rats were killed on day 7. Wnt/β-catenin-related genes and expression of proteins was determined using real-time PCR, western blotting, and immunohistochemistry. Among 13 genes identified by real-time PCR, seven genes were upregulated in diabetic rats compared with control animals including the target genes c-Myc and Tcf4. Diabetic rats also showed a significant increase in β-catenin protein compared with control animals. Treatment of diabetic rats attenuated the stimulating effect of diabetes on intestinal cell proliferation and Wnt/β-catenin signaling. In conclusion, enhanced intestinal epithelial cell proliferation in diabetic rats correlates with β-catenin accumulation.

scholarly journals Intestinal Epithelial Cell Tyrosine Kinase 2 Transduces IL-22 Signals To Protect from Acute Colitis

2015 ◽  
Vol 195 (10) ◽  
pp. 5011-5024 ◽  
Author(s):  
Eva Hainzl ◽  
Silvia Stockinger ◽  
Isabella Rauch ◽  
Susanne Heider ◽  
David Berry ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Tyrosine Kinase ◽  
Intestinal Epithelial Cell ◽  
Intestinal Epithelial ◽  
Acute Colitis ◽  
Tyrosine Kinase 2
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