TEMPERATURE AND MYOGENIC FACTOR TRANSCRIPT LEVELS DURING EARLY DEVELOPMENT DETERMINES MUSCLE GROWTH POTENTIAL IN RAINBOW TROUT(ONCORHYNCHUS MYKISS) AND SEA BASS (DICENTRARCHUS LABRAX)

2001 ◽  
Vol 204 (16) ◽  
pp. 2763-2771 ◽  
Author(s):  
D. WILKES ◽  
S. Q. XIE ◽  
N. C. STICKLAND ◽  
H. ALAMI-DURANTE ◽  
M. KENTOURI ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Muscle Fibre ◽  
Early Development ◽  
Sea Bass ◽  
Growth Potential ◽  
Mrna Levels ◽  
European Sea Bass ◽  
Transcript Levels ◽  
Fibre Number ◽  
Sea Bass Larvae

SUMMARY The influence of changes in environmental temperature on the mRNA levels of myogenic regulatory factors (MRFs), i.e. MyoD and myogenin, as well as myosin heavy chain (MyHC) were studied during early larval development in rainbow trout and sea bass. Phosphoimager analysis of northern blots indicated that there is an optimum temperature for the RNA transcript levels of MRF and MyHC RNA in trout and in sea bass larvae. In the trout strain studied, the highest concentration for MRF and MyHC transcripts was found at 8°C rather than 4°C or 20°C. In European sea bass, the highest concentrations of MRF and MyHC mRNA were observed at 15-20°C rather than 13°C. Raising sea bass larvae at 15°C was associated with higher MyHC gene expression as well as a trend towards an increase in total muscle fibre number and higher growth rates after transfer at ambient temperature. Results suggest that mRNA levels of MRF and MyHC can be used to optimise early development. An experiment in which the temperature was changed illustrates the consequence of precise temporal expression of MRF genes in specifying muscle fibre number at critical stages during early development.

scholarly journals Nutritional programming by dietary carbohydrates in European sea bass larvae: Not always what expected at juvenile stage

Aquaculture ◽  
2019 ◽  
Vol 501 ◽  
pp. 441-447 ◽  
Author(s):  
J.L. Zambonino-Infante ◽  
S. Panserat ◽  
A. Servili ◽  
O. Mouchel ◽  
L. Madec ◽  
...  
Keyword(s):  
Sea Bass ◽  
Juvenile Stage ◽  
Dietary Carbohydrates ◽  
European Sea Bass ◽  
Nutritional Programming ◽  
Sea Bass Larvae

scholarly journals Intestinal B0AT1 (SLC6A19) and PEPT1 (SLC15A1) mRNA levels in European sea bass (Dicentrarchus labrax) reared in fresh water and fed fish and plant protein sources

2015 ◽  
Vol 4 ◽  
Author(s):  
Simona Rimoldi ◽  
Elena Bossi ◽  
Sheenan Harpaz ◽  
Anna Giulia Cattaneo ◽  
Giovanni Bernardini ◽  
...  
Keyword(s):  
Dicentrarchus Labrax ◽  
Sea Bass ◽  
Conversion Ratio ◽  
Feed Conversion Ratio ◽  
Fish Growth ◽  
Mrna Levels ◽  
Feed Conversion ◽  
European Sea Bass ◽  
Protein Sources ◽  
Plant Protein Sources

AbstractThe objective of the present study was to examine the effect of diets with descending fish meal (FM) inclusion levels and the addition of salt to the diet containing the lowest FM level on growth performances, feed conversion ratio, and intestinal solute carrier family 6 member 19 (SLC6A19) and oligopeptide transporter 1 (PEPT1) transcript levels, in freshwater-adapted European sea bass (Dicentrarchus labrax). We first isolated by molecular cloning and sequenced a full-length cDNA representing the neutral amino acid transporter SLC6A19 in sea bass. The cDNA sequence was deposited in GenBank database (accession no. KC812315). The twelve transmembrane domains and the ‘de novo’ prediction of the three-dimensional structure of SLC6A19 protein (634 amino acids) are presented. We then analysed diet-induced changes in the mRNA copies of SLC6A19 and PEPT1 genes in different portions of sea bass intestine using real-time RT-PCR. Sea bass were fed for 6 weeks on different diets, with ascending levels of fat or descending levels of FM, which was replaced with vegetable meal. The salt-enriched diet was prepared by adding 3 % NaCl to the diet containing 10 % FM. SLC6A19 mRNA in the anterior and posterior intestine of sea bass were not modulated by dietary protein sources and salt supplementation. Conversely, including salt in a diet containing a low FM percentage up-regulated the mRNA copies of PEPT1 in the hindgut. Fish growth correlated positively with the content of FM in the diets. Interestingly, the addition of salt to the diet containing 10 % FM improved feed intake, as well as specific growth rate and feed conversion ratio.

scholarly journals The development of contemporary European sea bass larvae (Dicentrarchus labrax) is not affected by projected ocean acidification scenarios

2017 ◽  
Vol 164 (7) ◽  
Author(s):  
Amélie Crespel ◽  
José-Luis Zambonino-Infante ◽  
David Mazurais ◽  
George Koumoundouros ◽  
Stefanos Fragkoulis ◽  
...  
Keyword(s):  
Ocean Acidification ◽  
Dicentrarchus Labrax ◽  
Sea Bass ◽  
European Sea Bass ◽  
Sea Bass Larvae
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