Nutritional evaluation of differently processed plant protein sources on the performance of broiler chickens

An eight-week feeding trial was conducted to assess four differently protein sources on performance of broiler chickens. Two hundred and fifty (250) day old unsex Anak 2000 broiler chicks were randomly allotted to four differently processed plant protein sources i.e. cooked soybean, salt treated African locust bean and sprouted Bambara nut with groundnut cake (GNC) based diet as control, the treatments were replicated five times (12 birds per replicate) in a Completely Randomized Block Design (CRBD). Results showed that daily feed intake, daily weight gain and feed conversion ratio were affected by different protein sources (p<0.05) at starter, finisher and overall phase. The carcass characteristics showed that live weight (1988-2160 g), slaughter weight (1610-1965 g) and plucked weight (1600-1814 g) were affected by different protein sources(p<0.05). The dressing percentage (83.31-86.25%) are not affected by different protein sources (p>0.05). The abdominal fat (0.61-0.64%), gizzard (1.61-1.77%), small intestine (40.80-43.20 cm) and large intestine (141.80-201.60 cm) were affected by the different protein sources (p<0.05). All the prime cuts were significantly (p<0.05) affected by the dietary protein sources. Total feed cost is highest in soya-bean based diet (N 379.30). The highest body weight (4.53 kg) is in soya bean-based diet. The feed cost per kg gain is highest in GNC based diet (N 158.34) and lowest in soya bean based diet (N 126.43). All the protein sources enhanced the growth performance of broiler chickens; however, soybean was more effective in enhancing the performance of broiler chickens.

Factors Influencing Proteolysis and Protein Utilization in the Intestine of Pigs: A Review

Pigs are among the most important farm animals for meat production worldwide. In order to meet the amino acid requirements of the animals, pigs rely on the regular intake of proteins and amino acids with their feed. Unfortunately, pigs excrete about two thirds of the used protein, and production of pork is currently associated with a high emission of nitrogen compounds resulting in negative impacts on the environment. Thus, improving protein efficiency in pigs is a central aim to decrease the usage of protein carriers in feed and to lower nitrogen emissions. This is necessary as the supply of plant protein sources is limited by the yield and the cultivable acreage for protein plants. Strategies to increase protein efficiency that go beyond the known feeding options have to be investigated considering the characteristics of the individual animals. This requires a deep understanding of the intestinal processes including enzymatic activities, capacities of amino acid transporters and the microbiome. This review provides an overview of these physiological factors and the respective analyses methods.

The Spleen as a Target to Characterize Immunomodulatory Effects of Down-Stream Processed Cyberlindnera jadinii Yeasts in Atlantic Salmon Exposed to a Dietary Soybean Meal Challenge

Aquaculture feeds have changed dramatically from being largely based on fishmeal (FM) towards increased use of plant protein sources, which could impact the fish’s immune response. In order to characterize immunomodulatory properties of novel functional ingredients, this study used four diets, one based on FM, a challenging diet with 40% soybean meal (SBM), and two diets containing 40% SBM with 5% of Cyberlindnera jadinii yeast exposed to different down-stream processing conditions: heat-inactivated (ICJ) or autolysation (ACJ). The immunomodulatory effects of the diets were analyzed in the spleen of Atlantic salmon after 37 days of feeding, using a transcriptomic evaluation by RNA sequencing (RNA-seq) and the detection of specific immunological markers at the protein level through indirect Enzyme-linked Immunosorbent Assay (indirect ELISA). The results showed that SBM (compared to FM) induced a down-regulation of pathways related to ion binding and transport, along with an increase at the protein level of pro-inflammatory cytokines such as tumor necrosis factor alpha (TNFα) and interferon gamma (IFNγ). On the other hand, while ICJ (compared to FM-group) maintain the inflammatory response associated with SBM, with higher levels of TNFα and IFNγ, and with an upregulation of creatine kinase activity and phosphagen metabolic process, the inclusion of ACJ was able to modulate the response of Atlantic salmon compared to fish fed the SBM-diet by the activation of biological pathways related to endocytosis, Pattern recognition receptor (PPRs)-signal transduction and transporter activity. In addition, ACJ was also able to control the pro-inflammatory profile of SBM, increasing Interleukin 10 (IL-10) levels and decreasing TNFα production, triggering an immune response similar to that of fish fed an FM-based diet. Finally, we suggest that the spleen is a good candidate to characterize the immunomodulatory effects of functional ingredients in Atlantic salmon. Moreover, the inclusion of ACJ in fish diets, with the ability to control inflammatory processes, could be considered in the formulation of sustainable salmon feed.

Carcass Traits, Organ Proportion and Bio-Economic Cost Benefits Analysis of Broiler Chickens Fed Different Dietary Plant Protein Sources In Sorghum-Based Diet

A 56-day study was carried out to evaluate the carcass characteristics and organ proportions of broiler chickens fed sorghum-based diets with varying plant protein. A total of 120 day-old broiler chickens from a reputable and disease free farm were used for this study. There were four treatments, each having thirty broiler birds replicated thrice with ten broiler birds per replicate using a Completely Randomized Design (CRD). The broiler birds were offered feed and water ad libitum. The growth parameters were taken on weekly basis. Carcass evaluation was carried out at the end of the trial. One bird per replicate was randomly selected, fasted over-night and slaughtered by severing the jugular vein. After scalding in warm water for about a minute, the feathers were manually plucked, each bird was cut into parts for carcass evaluation. The relative weights of the cut parts were as a percentage of dressed weight. The internal organs, such as heart, kidney, gizzard/proventriculus were all weighed separately and recorded using electric micrometer (3000g weighing gauge). The findings from this study showed that all the carcass parameters considered showed significant differences (p<0.05). Birds fed diet 3 gave the best live weight, slaughtered weight, defeathered weight, dressed weight and commensurate cut-parts. Soybean (whether cake or full-fat) demonstrate superiority over groundnut cake and cashew nut cake. Birds fed diet 3 gave the highest value of thigh, breast -cut, drumstick and wings, while birds fed diets 2, 1 and 4 gave less. The organ proportions of broiler chickens fed different dietary plant sources have all the parameters significantly difference (p<0.05), except the lungs though without any definite pattern. Average weight gain and feed conversion ratio were better (p<0.05) among birds fed diet 2, and 3. Cost benefits analysis per kilogram in naira terms for birds fed different plant protein sources in a sorghum-based diets were significant (p<0.05) among treatment levels. Least cost per kg feed was obtained in diet 1 (p<0.05). Cost of weight gained was (p<0.05) low in T1. Cost of feed consumed was low for diet T1 and T3. Revenue, gross margin and cost benefit ratio estimated were found to high (p<0.05) among T2 and T3. Broiler chickens fed diet 3 gave the best parameters. The actual contributions of the experiment, is to alternatively replace corn with sorghum completely at least cost with optimum performance.

A replacement of plant protein sources as an alternative of fish meal ingredient for African catfish, Clarias gariepinus: A review

Aquaculture is one of the fastest growing industries in the world. This industry supplies half of thefisheries products consumed annually, and future global demand can only be supplied by increasingaquaculture production, which, in turn, requires more aquafeed. Alternative ingredients must beincreasingly used to supply significant proportions of protein and energy in aquafeeds, creatingboth challenges and opportunities for researchers and industry. Nowadays, there are too manyresearches on fish disease, growth and health but less in a fish feed especially a replacement ofprotein from plant sources in the fish feed. One of the alternative ways to optimise the growth andhealth condition of the fish is provide them a good protein from plant as a replacement in fish mealwith the natural source protein instead of giving a good rearing condition and caring of the fish.The present article was constructed to highlight a replacement of plant protein sources as analternative of fish meal for fish feed in African catfish, Clarias gariepinus that has been done byresearchers. However, the application of replacement from plant protein sources in fish meal is nowgradually gaining importance in commercial aquaculture practices and opened the door for theresearchers to expend of this application, thus more detailed studies on molecular basis should belooked as to get the quality and enough nutrient fish feed for good production of aquacultureindustry.

A Rapid Gas-Chromatography/Mass-Spectrometry Technique for Determining Odour Activity Values of Volatile Compounds in Plant Proteins: Soy, and Allergen-Free Pea and Brown Rice Protein

Plant-based protein sources have a characteristic aroma that limits their usage in various meat-alternative formulations. Despite being the most popular plant-based protein, the allergenicity of soy protein severely restricts the potential adoption of soy protein as an animal substitute. Thereby, allergen-free plant-protein sources need to be characterized. Herein, we demonstrate a rapid solid-phase-microextraction gas-chromatography/mass-spectrometry (SPME-GC/MS) technique for comparing the volatile aroma profile concentration of two different allergen-free plant-protein sources (brown rice and pea) and comparing them with soy protein. The extraction procedure consisted of making a 1:7 w/v aqueous plant protein slurry, and then absorbing the volatile compounds on an SPME fibre under agitation for 10 min at 40 °C, which was subsequently injected onto a GC column coupled to an MS system. Observed volatile concentrations were used in conjunction with odour threshold values to generate a Total Volatile Aroma Score for each protein sample. A total of 76 volatile compounds were identified. Aldehydes and furans were determined to be the most dominant volatiles present in the plant proteins. Both brown rice protein and pea protein contained 64% aldehydes and 18% furans, with minor contents of alcohols, ketones and other compounds. On the other hand, soy protein consisted of fewer aldehydes (46%), but a more significant proportion of furans (42%). However, in terms of total concentration, brown rice protein contained the highest intensity and number of volatile compounds. Based on the calculated odour activity values of the detected compounds, our study concludes that pea proteins could be used as a suitable alternative to soy proteins in applications for allergen-free vegan protein products without interfering with the taste or flavour of the product.

Potential application and beneficial effects of a marine microalgal biomass produced in a high-rate algal pond (HRAP) in diets of European sea bass, Dicentrarchus labrax

Microalgae have been used as live food in aquatic species. In recent years, the interest in microalgae has considerably increased, thanks to the evolution of production techniques that have identified them as an ecologically attractive aquafeed ingredient. The present study provides the first data about the effects of dietary inclusion of a microalgae consortium grown in a high-rate algal pond system on zootechnical performance, morphometric indices, and dietary nutrient digestibility as well as morphology and functionality of the digestive system of European sea bass, Dicentrarchus labrax. A dietary treatment including a commercial mono-cultured microalgae (Nannochloropsis sp.) biomass was used for comparison. Six hundred and thirty-six European sea bass juveniles (18 ± 0.28 g) were randomly allotted into 12 experimental groups and fed 4 different diets for 10 weeks: a control diet based on fish meal, fish oil, and plant protein sources; a diet including 10% of Nannochloropsis spp. biomass (100 g/kg diet); and two diets including two levels (10% and 20%) of the microalgal consortium (100 and 200 g/kg diet). Even at the highest dietary inclusion level, the microalgal consortium (200 g/kg diet) did not affect feed palatability and fish growth performance. A significant decrease in the apparent digestibility of dry matter, protein, and energy was observed in diets including 10 and 20% of the microalgal consortium, but all fish exhibited a well-preserved intestinal histomorphology. Moreover, dietary inclusion with the microalgal consortium significantly increased the enzymatic activity of maltase, sucrase-isomaltase, and ɤ-glutamil transpeptidase in the distal intestine of the treated European sea bass. Algal consortium grown using fish farm effluents represents an attempt to enhance the utilization of natural biomasses in aquafeeds when used at 10 % as substitute of vegetable ingredients in diet for European sea bass.

A review on the use of dried distiller’s grains with solubles (DDGS) in aquaculture feeds

Distiller’s dried grains with soluble (DDGS) to replace fishmeal (FM) or other plant protein sources and its effects on an aquatic animal’s growth performance and health system is rarely discussed. In recent times FM use in aquafeed has been a problem for the aquaculture industry because of the depletion of fish resources in the ocean and the high cost of FM. For that reason, researchers are focusing on reducing the cost of aquafeed production by lowering the inclusion of FM in aquafeed. One of the low-cost alternative protein sources to replace FM is DDGS. The present review discusses the compilation of available literature review and other works on the use of DDGS to replace FM in aquafeed and how it improves the growth performance, immune health system, and disease resistance in an aquatic animal. It focuses on the production of DDGS, the nutritional composition of DDGS, the chemical and physical characteristics of DDGS, and the effects of the DDGS inclusion in aquafeed on the aquatic animal. Apart from improving the growth performance of the aquatic animal when included in aquafeed, it also helps improve the immune health system of the animal. It also helps boost the immune system of the aquatic animal to fight diseases and increase disease resistance when included in aquafeed. Furthermore, DDGS also contains some constituents like carotenoids that help improve the meat quality of the animal when included in aquafeed. The current review also discusses the management and use of technology to improve the aquaculture industry, educate farmers on reducing diseases during the culturing periods, and increase productivity and profit in a friendly environment.

Plant Proteins and Exercise: What Role Can Plant Proteins Have in Promoting Adaptations to Exercise?

Adequate dietary protein is important for many aspects of health with current evidence suggesting that exercising individuals need greater amounts of protein. When assessing protein quality, animal sources of protein routinely rank amongst the highest in quality, largely due to the higher levels of essential amino acids they possess in addition to exhibiting more favorable levels of digestibility and absorption patterns of the amino acids. In recent years, the inclusion of plant protein sources in the diet has grown and evidence continues to accumulate on the comparison of various plant protein sources and animal protein sources in their ability to stimulate muscle protein synthesis (MPS), heighten exercise training adaptations, and facilitate recovery from exercise. Without question, the most robust changes in MPS come from efficacious doses of a whey protein isolate, but several studies have highlighted the successful ability of different plant sources to significantly elevate resting rates of MPS. In terms of facilitating prolonged adaptations to exercise training, multiple studies have indicated that a dose of plant protein that offers enough essential amino acids, especially leucine, consumed over 8–12 weeks can stimulate similar adaptations as seen with animal protein sources. More research is needed to see if longer supplementation periods maintain equivalence between the protein sources. Several practices exist whereby the anabolic potential of a plant protein source can be improved and generally, more research is needed to best understand which practice (if any) offers notable advantages. In conclusion, as one considers the favorable health implications of increasing plant intake as well as environmental sustainability, the interest in consuming more plant proteins will continue to be present. The evidence base for plant proteins in exercising individuals has seen impressive growth with many of these findings now indicating that consumption of a plant protein source in an efficacious dose (typically larger than an animal protein) can instigate similar and favorable changes in amino acid update, MPS rates, and exercise training adaptations such as strength and body composition as well as recovery.

Abstract MP55: Red And Processed Meat And Alternative Protein Sources In Relation To Risk Of Fatal Coronary Heart Disease: A Pooled Analysis Of 16 Prospective Cohort Studies

Background: Red and processed meat intake have been associated with higher risk of fatal coronary heart disease (CHD). However, very few studies have evaluated substitution of alternative protein sources for red and processed meat in relation to fatal CHD risk. Objective: We estimated the associations of substituting other animal and plant protein sources for total red meat, unprocessed red meat, and processed meat in relation to risk of fatal CHD. Methods: This pooled analysis was conducted within the Pooling Project of Prospective Studies of Diet and Cancer and included participants with no prior self-reported history of cardiovascular diseases or cancer. Diet was assessed by cohort-specific food frequency questionnaires collected at baseline. Total red meat included processed meat and unprocessed red meat. Animal protein sources included seafood, poultry, eggs, and low- and high-fat dairy products; plant protein sources included nuts and beans. After adjusting for dietary and non-dietary factors, study-specific relative risks (RR) and 95% confidence intervals (CI) were calculated using Cox proportional hazard models and then pooled using a random effects model. Isocaloric dietary substitution was evaluated by differences in coefficients in models including red meat and all alternative protein sources described above as continuous variables. Results: In preliminary analyses of 16 prospective cohorts including 1,364,211 participants (40% men and mean age of 57 ± 10 years) followed for a maximum of 32 years across studies, we identified 51,176 fatal CHD cases. A 100g/day increment in total red meat intake was associated with a 7% higher risk of fatal CHD, RR=1.07 (95% CI 1.04, 1.10). Substitutions of 200 kcal/day from nuts, low- and high-fat dairy products, and poultry for 200 kcal/d from total red meat were associated with 6-14% lower risks of fatal CHD. These associations were stronger when substituting the alternative protein sources for processed meat, especially among women (n=14,888 cases, 17-24% lower risk). Substituting 200 kcal/day from eggs for 200 kcal/day from total red meat and unprocessed red meat was associated with 8 and 14% higher risk of fatal CHD, respectively; this substitution for processed meat was weaker and not significant (4%). Results were similar after excluding the first 4 years of follow-up. Conclusions: Preliminary results from this international pooled analysis of 16 cohort studies provide more evidence about the risks of red and processed meat consumption. Replacement of red and processed meat with nuts, dairy products, or poultry may reduce the risk of fatal CHD.