scholarly journals Dose-Finding Study of a CEA-Targeting Agent, SGM-101, for Intraoperative Fluorescence Imaging of Colorectal Cancer

2020 ◽  
Author(s):  
Kim S. de Valk ◽  
Marion M. Deken ◽  
Dennis P. Schaap ◽  
Ruben P. Meijer ◽  
Leonora S. Boogerd ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Fluorescence Imaging ◽  
Near Infrared ◽  
Imaging System ◽  
Dose Finding ◽  
Phase Iii ◽  
Imaging Dose ◽  
Intraoperative Fluorescence Imaging ◽  
Optimal Imaging ◽  
Intraoperative Fluorescence

Abstract Background Carcinoembryonic antigen is overexpressed in colorectal cancer (CRC), making it an optimal target for fluorescence imaging. A phase I/II study was designed to determine the optimal imaging dose of SGM-101 for intraoperative fluorescence imaging of primary and recurrent CRC. Methods Patients were included and received a single dose of SGM-101 at least 24 h before surgery. Patients who received routine anticancer therapy (i.e., radiotherapy or chemotherapy) also were eligible. A dedicated near-infrared imaging system was used for real-time fluorescence imaging during surgery. Safety assessments were performed and SGM-101 efficacy was evaluated per dose level to determine the most optimal imaging dose. Results Thirty-seven patients with CRC were included in the analysis. Fluorescence was visible in all primary and recurrent tumors. In seven patients, no fluorescence was seen; all were confirmed as pathological complete responses after neoadjuvant therapy. Two tumors showed false-positive fluorescence. In the 37 patients, a total of 97 lesions were excised. The highest mean intraoperative tumor-to-background ratio (TBR) of 1.9 (p = 0.019) was seen in the 10-mg dose. This dose showed a sensitivity of 96%, specificity of 63%, and negative predictive value of 94%. Nine patients (24%) had a surgical plan alteration based on fluorescence, with additional malignant lesions detected in six patients. Conclusions The optimal imaging dose was established at 10 mg 4 days before surgery. The results accentuate the potential of SGM-101 and designated a promising base for the multinational phase III study, which enrolled the first patients in June 2019.

Real-time intraoperative fluorescence imaging system using light-absorption correction

2009 ◽  
Vol 14 (6) ◽  
pp. 064012 ◽  
Author(s):  
George Themelis ◽  
Jung Sun Yoo ◽  
Kwang-Sup Soh ◽  
Ralf Schulz ◽  
Vasilis Ntziachristos
Keyword(s):  
Real Time ◽  
Fluorescence Imaging ◽  
Light Absorption ◽  
Imaging System ◽  
Absorption Correction ◽  
Fluorescence Imaging System ◽  
Intraoperative Fluorescence Imaging ◽  
Intraoperative Fluorescence

scholarly journals In Search for Optimal Targets for Intraoperative Fluorescence Imaging of Peritoneal Metastasis From Colorectal Cancer

2017 ◽  
Vol 9 ◽  
pp. 1179299X1772825 ◽  
Author(s):  
Charlotte ES Hoogstins ◽  
Benjamin Weixler ◽  
Leonora SF Boogerd ◽  
Diederik J Hoppener ◽  
Hendrica AJM Prevoo ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Fluorescence Imaging ◽  
Peritoneal Metastasis ◽  
Cell Adhesion Molecule ◽  
Folate Receptor ◽  
Epithelial Cell Adhesion Molecule ◽  
Tissue Samples ◽  
Benign Tissue ◽  
Intraoperative Fluorescence Imaging ◽  
Intraoperative Fluorescence

Peritoneal metastasis (PM) occurs in about 10% of patients with colorectal cancer (CRC). Fluorescence imaging can enhance contrast between cancerous and benign tissue, enabling the surgeon to clearly visualize PM during cytoreductive surgery. This study assessed the suitability of different biomarkers as potential targets for tumor-targeted imaging of PM of CRC. Tissue samples from primary tumor and PM from patients with CRC were obtained from the pathology archives and immunohistochemical stainings were performed. Overexpression of the epithelial cell adhesion molecule (EpCAM) and carcinoembryonic antigen (CEA) was seen in 100% of PM samples and the expression was strong in >70% of samples. Tyrosine-kinase Met (C-Met) and folate receptor α overexpression was seen in 20% of PM samples. For successful application of tumor-targeted intraoperative fluorescence imaging of PM, biomarkers need to be identified. We demonstrated that both EpCAM and CEA are suitable targets for fluorescence imaging of PM in patients with CRC.

scholarly journals Near Infrared Fluorescence Imaging of Intraperitoneal Ovarian Tumors in Mice Using Erythrocyte-Derived Optical Nanoparticles and Spatially-Modulated Illumination

Cancers ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 2544
Author(s):  
Joshua M. Burns ◽  
Elise Shafer ◽  
Raviraj Vankayala ◽  
Vikas Kundra ◽  
Bahman Anvari
Keyword(s):  
Fluorescence Imaging ◽  
Cytoreductive Surgery ◽  
Animal Studies ◽  
Near Infrared ◽  
Gynecological Cancer ◽  
Ovarian Tumors ◽  
Structured Illumination ◽  
Spatial Frequencies ◽  
Imaging Probes ◽  
Intraoperative Fluorescence Imaging

Ovarian cancer is the deadliest gynecological cancer. Cytoreductive surgery to remove primary and intraperitoneal tumor deposits remains as the standard therapeutic approach. However, lack of an intraoperative image-guided approach to enable the visualization of all tumors can result in incomplete cytoreduction and recurrence. We engineered nano-sized particles derived from erythrocytes that encapsulate the near infrared (NIR) fluorochrome, indocyanine green, as potential imaging probes for tumor visualization during cytoreductive surgery. Herein, we present the first demonstration of the use of these nanoparticles in conjunction with spatially-modulated illumination (SMI), at spatial frequencies in the range of 0–0.5 mm−1, to fluorescently image intraperitoneal ovarian tumors in mice. Results of our animal studies suggest that the nanoparticles accumulated at higher levels within tumors 24 h post-intraperitoneal injection as compared to various other organs. We demonstrate that, under the imaging specifications reported here, use of these nanoparticles in conjunction with SMI enhances the fluorescence image contrast between intraperitoneal tumors and liver, and between intraperitoneal tumors and spleen by nearly 2.1, and 3.0 times, respectively, at the spatial frequency of 0.2 mm−1 as compared to the contrast values at spatially-uniform (non-modulated) illumination. These results suggest that the combination of erythrocyte-derived NIR nanoparticles and structured illumination provides a promising approach for intraoperative fluorescence imaging of ovarian tumor nodules at enhanced contrast.

Dual-emissive quantum dots for multispectral intraoperative fluorescence imaging

Biomaterials ◽  
2010 ◽  
Vol 31 (26) ◽  
pp. 6823-6832 ◽  
Author(s):  
Patrick T.K. Chin ◽  
Tessa Buckle ◽  
Arantxa Aguirre de Miguel ◽  
Stefan C.J. Meskers ◽  
René A.J. Janssen ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Fluorescence Imaging ◽  
Intraoperative Fluorescence Imaging ◽  
Intraoperative Fluorescence

scholarly journals Tissue-like phantoms for near-infrared fluorescence imaging system assessment and the training of surgeons

2006 ◽  
Vol 11 (1) ◽  
pp. 014007 ◽  
Author(s):  
Alec M. De Grand ◽  
Stephen J. Lomnes ◽  
Deborah S. Lee ◽  
Matthew Pietrzykowski ◽  
Shunsuke Ohnishi ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Near Infrared ◽  
Imaging System ◽  
Near Infrared Fluorescence ◽  
Fluorescence Imaging System ◽  
Training Of Surgeons ◽  
System Assessment ◽  
Near Infrared Fluorescence Imaging

Highly stable near-infrared dye conjugated cerasomes for fluorescence imaging-guided synergistic chemo-photothermal therapy of colorectal cancer

2019 ◽  
Vol 7 (7) ◽  
pp. 2873-2888 ◽  
Author(s):  
Xu Zhang ◽  
Xiaolong Liang ◽  
Xiaotu Ma ◽  
Rui Hou ◽  
Xiaoda Li ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Drug Release ◽  
Fluorescence Imaging ◽  
Photothermal Therapy ◽  
High Stability ◽  
Near Infrared ◽  
Great Promise ◽  
Near Infrared Dye

Dye-conjugated cerasome loaded with DOX exhibited high stability and controllable drug release, holding great promise in colorectal cancer photothermal chemotherapy.

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