scholarly journals Hormonal Regulation of Connexin 43 Expression and Gap Junctional Communication in Human Osteoblastic Cells.

1994 ◽  
Vol 19 (3) ◽  
pp. 173-177 ◽  
Author(s):  
Hideki Chiba ◽  
Norimasa Sawada ◽  
Masahito Oyamada ◽  
Takashi Kojima ◽  
Kousuke Iba ◽  
...  
Keyword(s):  
Hormonal Regulation ◽  
Connexin 43 ◽  
Osteoblastic Cells ◽  
Gap Junctional Communication ◽  
Junctional Communication ◽  
Gap Junctional ◽  
Human Osteoblastic Cells

Gap-junctional communication is required for the maturation process of osteoblastic cells in culture

Bone ◽  
2001 ◽  
Vol 28 (4) ◽  
pp. 362-369 ◽  
Author(s):  
P.C Schiller ◽  
G D’Ippolito ◽  
W Balkan ◽  
B.A Roos ◽  
G.A Howard
Keyword(s):  
Osteoblastic Cells ◽  
Maturation Process ◽  
Gap Junctional Communication ◽  
Cells In Culture ◽  
Junctional Communication ◽  
Gap Junctional

Inhibition of gap junctional communication by polyunsaturated fatty acids in WB cells: evidence that connexin 43 is not hyperphosphorylated

1995 ◽  
Vol 16 (7) ◽  
pp. 1505-1511 ◽  
Author(s):  
Charles S. T. Hii ◽  
Antonio Ferrante ◽  
Simon Schmidt ◽  
Deborah A. Rathjen ◽  
Brenton S. Robinson ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Polyunsaturated Fatty Acids ◽  
Connexin 43 ◽  
Gap Junctional Communication ◽  
Junctional Communication ◽  
Gap Junctional

scholarly journals Polyamines preserve connexin 43-mediated gap junctional communication during intracellular hypercalcemia and acidosis

Neuroreport ◽  
2017 ◽  
Vol 28 (4) ◽  
pp. 208-213 ◽  
Author(s):  
Lilia Y. Kucheryavykh ◽  
Jan Benedikt ◽  
Luis A. Cubano ◽  
Serguei N. Skatchkov ◽  
Feliksas F. Bukauskas ◽  
...  
Keyword(s):  
Connexin 43 ◽  
Gap Junctional Communication ◽  
Junctional Communication ◽  
Gap Junctional

scholarly journals Involvement of connexin 43 phosphorylation and gap junctional communication between smooth muscle cells in vasopressin-induced ROCK-dependent vasoconstriction after hemorrhagic shock

2017 ◽  
Vol 313 (4) ◽  
pp. C362-C370 ◽  
Author(s):  
Guangming Yang ◽  
Xiaoyong Peng ◽  
Yue Wu ◽  
Tao Li ◽  
Liangming Liu
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Gap Junctions ◽  
Hemorrhagic Shock ◽  
Connexin 43 ◽  
Muscle Cells ◽  
Gap Junctional Communication ◽  
Contractile Effect ◽  
Junctional Communication ◽  
Gap Junctional

We examined the roles played by gap junctions (GJs) and the GJ channel protein connexin 43 (Cx43) in arginine vasopressin (AVP)-induced vasoconstriction after hemorrhagic shock and their relationship to Rho kinase (ROCK) and protein kinase C (PKC). The results showed that AVP induced an endothelium-independent contraction in rat superior mesenteric arteries (SMAs). Blocking the GJs significantly decreased the contractile response of SMAs and vascular smooth muscle cells (VSMCs) to AVP after shock and hypoxia. The selective Cx43-mimetic peptide inhibited the vascular contractile effect of AVP after shock and hypoxia. AVP restored hypoxia-induced decrease of Cx43 phosphorylation at Ser262 and gap junctional communication in VSMCs. Activation of RhoA with U-46619 increased the contractile effect of AVP. This effect was antagonized by the ROCK inhibitor Y27632 and the Cx43-mimetic peptide. In contrast, neither an agonist nor an inhibitor of PKC had significant effects on AVP-induced contraction after hemorrhagic shock. In addition, silencing of Cx43 with siRNA blocked the AVP-induced increase of ROCK activity in hypoxic VSMCs. In conclusion, AVP-mediated vascular contractile effects are endothelium and myoendothelial gap junction independent. Gap junctions between VSMCs, gap junctional communication, and Cx43 phosphorylation at Ser262 play important roles in the vascular effects of AVP. RhoA/ROCK, but not PKC, is involved in this process.

Retinoid-enhanced gap junctional communication is achieved by increased levels of connexin 43 mRNA and protein

1990 ◽  
Vol 3 (6) ◽  
pp. 335-343 ◽  
Author(s):  
Michael Rogers ◽  
John M. Berestecky ◽  
Mohammad Z. Hossain ◽  
Huiming Guo ◽  
Ranjana Kadle ◽  
...  
Keyword(s):  
Connexin 43 ◽  
Gap Junctional Communication ◽  
Junctional Communication ◽  
Gap Junctional

Implication of gap junction coupling in amphibian vitellogenin uptake

Zygote ◽  
2007 ◽  
Vol 15 (2) ◽  
pp. 149-157 ◽  
Author(s):  
M.E. Mónaco ◽  
E.I. Villecco ◽  
S.S. Sánchez
Keyword(s):  
Gap Junctions ◽  
Connexin 43 ◽  
Physiological Role ◽  
Follicle Cell ◽  
Pcr Analysis ◽  
Dye Transfer ◽  
Gap Junctional Communication ◽  
Junctional Communication ◽  
Endocytic Activity ◽  
Gap Junctional

SummaryThe aim of the present study was to investigate the physiological role and the expression pattern of heterologous gap junctions during Xenopus laevis vitellogenesis. Dye transfer experiments showed that there are functional gap junctions at the oocyte/follicle cell interface during the vitellogenic process and that octanol uncouples this intercellular communication. The incubation of vitellogenic oocytes in the presence of biotinylated bovine serum albumin (b-BSA) or fluorescein dextran (FDX), showed that oocytes develop stratum of newly formed yolk platelets. In octanol-treated follicles no sign of nascent yolk sphere formation was observed. Thus, experiments in which gap junctions were downregulated with octanol showed that coupled gap junctions are required for endocytic activity. RT-PCR analysis showed that the expression of connexin 43 (Cx43) was first evident at stage II of oogenesis and increased during the subsequent vitellogenic stages (III, IV and V), which would indicate that this Cx is related to the process that regulates yolk uptake. No expression changes were detected for Cx31 and Cx38 during vitellogenesis. Based on our results, we propose that direct gap junctional communication is a requirement for endocytic activity, as without the appropriate signal from surrounding epithelial cells X. laevis oocytes were unable to endocytose VTG.

scholarly journals Transforming Growth Factor-β3 Increases Gap-Junctional Communication among Folliculostellate Cells to Release Basic Fibroblast Growth Factor

Endocrinology ◽  
2005 ◽  
Vol 146 (9) ◽  
pp. 4054-4060 ◽  
Author(s):  
Nurul Kabir ◽  
Kirti Chaturvedi ◽  
Lian Sheng Liu ◽  
Dipak K. Sarkar
Keyword(s):  
Growth Factor ◽  
Gap Junction ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Connexin 43 ◽  
Fibroblast Growth ◽  
Gap Junctional Communication ◽  
Junctional Communication ◽  
Gap Junctional ◽  
Gap Junction Inhibitor

Abstract Folliculostellate (FS) cells are known to communicate with each other and with endocrine cells via gap junctions in the anterior pituitary. We investigated whether TGFβ3 and estradiol, known to regulate FS cell production and secretion of basic fibroblast growth factor (bFGF), increases gap junctional communication to alter bFGF secretion from FS cells. FS cells in monolayer cultures were treated with TGFβ3 or vehicle alone for 24 h and then microinjected with Lucifer Yellow and high-molecular-weight Texas Red dextran. Ten minutes later the transfer of dye among adjacent cells was recorded with a digital microscope. TGFβ3 increased the transfer of dye. The TGFβ3-neutralizing antibody and the gap junction inhibitor octanol reduced the effect of TGFβ3 on the transfer of dye. The TGFβ3-induced transfer of dye was unaltered by simultaneous treatment with estradiol. The steroid alone also had no effect. TGFβ3 increased total and phosphorylated levels of connexin 43. Estradiol treatment did not produce any significant changes on basal or TGFβ3-induced increases in connexin 43 levels. The gap-junction inhibitor octanol reduced TGFβ3-increased levels of bFGF in FS cells. Taken together, these results suggest that TGFβ3 may act on FS cells to increase gap-junctional communication to maximize its effect on bFGF secretion.

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