Gene Expression of Lipid Binding Protein Transferred the Ability of Specific Attachment of Hemopoietic Cells to Non-supportive Stromal Cell Line, MS-K.

1997 ◽  
Vol 22 (6) ◽  
pp. 595-602
Author(s):  
Emi Takashita ◽  
Kenkichi Sugimoto ◽  
Huijie Jiang ◽  
Maki Tohma ◽  
Eriko Urano ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Line ◽  
Stromal Cell ◽  
Binding Protein ◽  
Lipid Binding ◽  
Hemopoietic Cells ◽  
Lipid Binding Protein ◽  
Specific Attachment ◽  
Stromal Cell Line

Differential gene expression by 1,25(OH) 2 D 3 in an endometriosis stromal cell line

2017 ◽  
Vol 173 ◽  
pp. 223-227 ◽  
Author(s):  
Sue Ann Ingles ◽  
Liang Wu ◽  
Benjamin T. Liu ◽  
Yibu Chen ◽  
Chun-Yeh Wang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Line ◽  
Differential Gene Expression ◽  
Stromal Cell ◽  
Stromal Cell Line ◽  
Differential Gene

The Mevalonate Pathway As a Metabolic Target to Circumvent Multidrug-Resistance in Chronic Lymphocytic Leukemia Cells

Blood ◽  
2011 ◽  
Vol 118 (21) ◽  
pp. 735-735
Author(s):  
Marta Coscia ◽  
Micol Rigoni ◽  
Chiara Riganti ◽  
Ivana Campia ◽  
Candida Vitale ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transcription Factor ◽  
Chronic Lymphocytic Leukemia ◽  
Cell Line ◽  
Stromal Cell ◽  
Rho Kinase ◽  
Lymphocytic Leukemia ◽  
Mdr1 Gene ◽  
Mdr1 Gene Expression ◽  
Stromal Cell Line

Abstract Abstract 735 The mutational status of the tumor immunoglobulin heavy chain variable region (IGHV) is a very reliable prognosticator in chronic lymphocytic leukemia (CLL): patients with unmutated (UM) IGHV have a worse prognosis than patients with mutated (M) IGHV. We have recently shown that UM CLL cells are more dependent on microenvironment-mediated signals for survival (Coscia M. et al, Leukemia, 2011), but it is currently unknown whether this also translates into an enhanced chemotherapy resistance. Multi drug resistance (MDR) is mediated by the over-expression of membrane transporters, like P-glycoprotein (Pgp), which actively extrudes several anticancer drugs. The Pgp protein is the product of the mdr1 gene whose regulation is under the positive control of the mevalonate (Mev) metabolic pathway, the downstream Ras/RhoA dependent signaling pathways and the transcription factor Hypoxia-Inducible-Factor-1-alfa (HIF-1α). The aim of this study was to investigate the MDR phenotype and signaling cascade in M and UM CLL cells under basal conditions and after exposure to prosurvival microenvironment-mediated signals. Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation from untreated CLL patients. More than 90% pure CLL cells were co-cultured with the murine stromal cell line M2-10B4. Culture conditions included: medium alone, Zoledronic acid (ZA) (1 μmol/L), ERK1/2 kinase inhibitor PD98059 (10 μmol/L) and the HIF-1α inhibitor YC-1 (10 μmol/L). The level of activity of the Mev pathway was evaluated by measuring the intermediate metabolite farnesyl pyrophosphate (FPP) and the final product cholesterol (Chol) as previously described (Coscia M. et al, J Cell Mol Med, 2010). The Ras and RhoA activity, was evaluated by measuring the GTP binding. Rho kinase activity was measured using the CycLex Rho Kinase Assay Kit (CycLex Co., Nagano). The following antibodies were used for Western Blot (WB) analyses: anti phospho-(Thr202/Tyr204, Thr185/Tyr187)-ERK1/2; anti-ERK 1/2; anti-HIF-1α; anti-Pgp; anti-GAPDH. The activity of HIF-1 in the nuclear extracts was assessed by the TransAM™ HIF-1 Transcription Factor Assay Kit (Active Motif). Mdr1 gene expression was evaluated by RT-PCR. Intracellular Doxorubicine (Doxo) accumulation was evaluated by cytofluorimetric analyses. We have found that the Mev pathway is significantly accelerated in freshly isolated UM vs M CLL cells. This hypermetabolic activity translates into a higher activation of the Ras/ERK-1/2 and RhoA/Rho kinase signaling pathways, and to a significantly more active HIF-1α in UM compared to M CLL cells. HIF-1α activation positively regulates mdr1 gene expression in UM CLL cells leading to Pgp expression and more effective Doxo extrusion in these cells. The co-culture of CLL cells with the murine stromal cell line M2-10B4 further accelerated the Mev pathway and activated the HIF-1α/mdr1/PgP axis leading to even lower intracellular Doxo retention. This stroma-mediated effect was particularly evident in UM CLL cells. Targeting of the Mev pathway with ZA and statins, and targeting of ERK-1/2 and HIF-1α with specific inhibitors (PD85 and YC1-10, respectively) significantly reduced basal and stroma-induced activity of HIF-1α/mdr1/PgP axis, and significantly increased the intracellular concentrations of Doxo in UM CLL cells. These data indicate that the pharmacological inhibition of the Mev pathway and of the downstream HIF-1α/mdr1/PgP axis can be regarded as a novel potential strategy to circumvent basal and environment-mediated chemoresistance of CLL cells. Disclosures: Massaia: Novartis Farma S.p.A: Honoraria, Research Funding, scientific adviser board.

Phosphate Depletion Enhances Tissue-Nonspecific Alkaline Phosphatase Gene Expression in a Cultured Mouse Marrow Stromal Cell Line ST2

1999 ◽  
Vol 265 (1) ◽  
pp. 24-28 ◽  
Author(s):  
Masae Goseki-Sone ◽  
Asako Yamada ◽  
Kumiko Asahi ◽  
Arisa Hirota ◽  
Ikuko Ezawa ◽  
...  
Keyword(s):  
Gene Expression ◽  
Alkaline Phosphatase ◽  
Cell Line ◽  
Stromal Cell ◽  
Marrow Stromal Cell ◽  
Stromal Cell Line ◽  
Phosphate Depletion ◽  
Tissue Nonspecific Alkaline Phosphatase

Lipid metabolism mediated by adipocyte lipid binding protein (ALBP/aP2) gene expression in human THP-1 macrophages

2006 ◽  
Vol 188 (1) ◽  
pp. 102-111 ◽  
Author(s):  
Yuchang Fu ◽  
Liehong Luo ◽  
Nanlan Luo ◽  
W. Timothy Garvey
Keyword(s):  
Gene Expression ◽  
Lipid Metabolism ◽  
Binding Protein ◽  
Lipid Binding ◽  
Lipid Binding Protein ◽  
Adipocyte Lipid Binding Protein

N5 endometrial stromal cell line: A model system to study decidual prolactin gene expression

1999 ◽  
Vol 35 (3) ◽  
pp. 150-154 ◽  
Author(s):  
A. K. Brar ◽  
Y. Kanda ◽  
C. A. Kessler ◽  
M. I. Cedars ◽  
S. Handwerger
Keyword(s):  
Gene Expression ◽  
Cell Line ◽  
Stromal Cell ◽  
Model System ◽  
Endometrial Stromal Cell ◽  
Prolactin Gene ◽  
Stromal Cell Line ◽  
Decidual Prolactin

Phosphate depletion enhances bone morphogenetic protein-4 gene expression in a cultured mouse marrow stromal cell line ST2

2002 ◽  
Vol 299 (3) ◽  
pp. 395-399 ◽  
Author(s):  
Masae Goseki-Sone ◽  
Asako Yamada ◽  
Ryoko Hamatani ◽  
Lena Mizoi ◽  
Tadahiro Iimura ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Line ◽  
Bone Morphogenetic Protein ◽  
Stromal Cell ◽  
Bone Morphogenetic Protein 4 ◽  
Marrow Stromal Cell ◽  
Morphogenetic Protein ◽  
Stromal Cell Line ◽  
Phosphate Depletion

Spontaneous in vitro angiogenesis in a trout pronephric stromal cell line (TPS), and in TPS-haemopoietic co-cultures

2000 ◽  
Vol 10 (1) ◽  
pp. 21-31 ◽  
Author(s):  
M.L Diago ◽  
P López-Fierro ◽  
B Razquin ◽  
A Villena
Keyword(s):  
Cell Line ◽  
Stromal Cell ◽  
Stromal Cell Line ◽  
In Vitro Angiogenesis
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