scholarly journals Localization of Cytoplasmic Dynein Light-intermediate Chain mRNA in the Rat Testis Using In Situ Hybridization.

1998 ◽  
Vol 23 (1) ◽  
pp. 9-15 ◽  
Author(s):  
Seishi Maeda ◽  
Sang-Yoon Nam ◽  
Masahiko Fujisawa ◽  
Nobuaki Nakamuta ◽  
Kenji Ogawa ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Cytoplasmic Dynein ◽  
Rat Testis ◽  
Intermediate Chain

In situ hybridization studies of UDP-glucuronosyltransferase UGT1A6 expression in rat testis and brain

2000 ◽  
Vol 59 (11) ◽  
pp. 1441-1444 ◽  
Author(s):  
Alexander Brands ◽  
Peter A Münzel ◽  
Karl Walter Bock
Keyword(s):  
In Situ Hybridization ◽  
Rat Testis ◽  
Udp Glucuronosyltransferase

scholarly journals Characterization of Cytoplasmic Dynein Light-intermediate Chain isoforms in Rat Testis.

1998 ◽  
Vol 23 (3) ◽  
pp. 169-178 ◽  
Author(s):  
Seishi Maeda ◽  
Sang-Yoon Nam ◽  
Masahiko Fujisawa ◽  
Nobuaki Nakamuta ◽  
Kenji Ogawa ◽  
...  
Keyword(s):  
Cytoplasmic Dynein ◽  
Rat Testis ◽  
Intermediate Chain

scholarly journals The 78,000 M(r) intermediate chain of Chlamydomonas outer arm dynein isa WD-repeat protein required for arm assembly.

1995 ◽  
Vol 129 (1) ◽  
pp. 169-178 ◽  
Author(s):  
C G Wilkerson ◽  
S M King ◽  
A Koutoulis ◽  
G J Pazour ◽  
G B Witman
Keyword(s):  
Protein Interactions ◽  
Sequence Comparison ◽  
Fragment Length Polymorphism ◽  
Cytoplasmic Dynein ◽  
Protein Protein Interactions ◽  
Alpha Tubulin ◽  
Restriction Fragment Length ◽  
Large Insertion ◽  
Intermediate Chain

We have isolated and sequenced a full-length cDNA clone encoding the 78,000 Mr intermediate chain (IC78) of the Chlamydomonas outer arm dynein. This protein previously was shown to be located at the base of the solubilized dynein particle and to interact with alpha tubulin in situ, suggesting that it may be involved in binding the outer arm to the doublet microtubule. The sequence predicts a polypeptide of 683 amino acids having a mass of 76.5 kD. Sequence comparison indicates that IC78 is homologous to the 69,000 M(r) intermediate chain (IC69) of Chlamydomonas outer arm dynein and to the 74,000 M(r) intermediate chain (IC74) of cytoplasmic dynein. The similarity between the chains is greatest in their COOH-terminal halves; the NH(2)-terminal halves are highly divergent. The COOH-terminal half of IC78 contains six short imperfect repeats, termed WD repeats, that are thought to be involved in protein-protein interactions. Although not previously reported, these repeated elements also are present in IC69 and IC74. Using the IC78 cDNA as a probe, we screened a group of slow-swimming insertional mutants and identified one which has a large insertion in the IC78 gene and seven in which the IC78 gene is completely deleted. Electron microscopy of three of these IC78 mutants revealed that each is missing the outer arm, indicating that IC78 is essential for arm assembly or attachment to the outer doublet. Restriction fragment length polymorphism mapping places the IC78 gene on the left arm of chromosome XII/XIII, at or near the mutation oda9, which also causes loss of the outer arm. Mutants with defects in the IC78 gene do not complement the oda9 mutation in stable diploids, strongly suggesting that ODA9 is the structural gene for IC78.

Localization of the Human Cytoplasmic Dynein Heavy Chain (DNECL) to 14qter by Fluorescence in Situ Hybridization

Genomics ◽  
1994 ◽  
Vol 22 (3) ◽  
pp. 660-661 ◽  
Author(s):  
D. Narayan ◽  
T. Desai ◽  
A. Banks ◽  
S.R. Patanjali ◽  
T.S. Ravikumar ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Heavy Chain ◽  
Cytoplasmic Dynein ◽  
Dynein Heavy Chain

Pro-opiomelanocortin (POMC) gene expression, as identified by in situ hybridization, in purified populations of interstitial macrophages and Leydig cells of the adult rat testis

1993 ◽  
Vol 5 (5) ◽  
pp. 545 ◽  
Author(s):  
H Li ◽  
GP Risbridger ◽  
JA Clements
Keyword(s):  
Gene Expression ◽  
In Situ Hybridization ◽  
Leydig Cells ◽  
Cell Types ◽  
Rat Testis ◽  
Adult Rat ◽  
Pomc Gene ◽  
Rna Probe ◽  
Silver Grains

The presence of testicular pro-opiomelanocortin (POMC) mRNA and POMC-derived peptides has recently been demonstrated in purified preparations of interstitial macrophages and in Leydig cells of the adult rat testis by Northern blot analysis and immunocytochemistry. In the present study, in situ hybridization provided further evidence that the POMC gene is expressed by both purified interstitial macrophages and Leydig cells. The cellular localization of the POMC transcripts was similar for both cell types, silver grains being predominantly located in the cytoplasm. The specificity of the labelling was demonstrated by the lack of silver grains in the preparations pretreated with RNAase or hybridized with an insulin cDNA probe, a gene known not to be expressed in these cell types. An additional control was provided by hybridization with a sense POMC RNA probe, which gave a less intense signal when compared with the antisense RNA probe under the same experimental conditions. The results confirm POMC gene expression in both macrophages and Leydig cells in the adult rat testis.

DNA sequence mapping in interphase and metaphase chromosomes by fluorescence in situ hybridization

1992 ◽  
Vol 50 (1) ◽  
pp. 496-497
Author(s):  
Barbara Trask ◽  
Susan Allen ◽  
Anne Bergmann ◽  
Mari Christensen ◽  
Anne Fertitta ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Dna Sequence ◽  
Dna Sequences ◽  
Dual Band ◽  
Nick Translation ◽  
Metaphase Chromosomes ◽  
Band Pass ◽  
Texas Red ◽  
Fluorescent Spot

Using fluorescence in situ hybridization (FISH), the positions of DNA sequences can be discretely marked with a fluorescent spot. The efficiency of marking DNA sequences of the size cloned in cosmids is 90-95%, and the fluorescent spots produced after FISH are ≈0.3 μm in diameter. Sites of two sequences can be distinguished using two-color FISH. Different reporter molecules, such as biotin or digoxigenin, are incorporated into DNA sequence probes by nick translation. These reporter molecules are labeled after hybridization with different fluorochromes, e.g., FITC and Texas Red. The development of dual band pass filters (Chromatechnology) allows these fluorochromes to be photographed simultaneously without registration shift.

Ultrastructural analysis of the spatial distribution of MRNA

1992 ◽  
Vol 50 (1) ◽  
pp. 552-553
Author(s):  
Gary Bassell ◽  
Robert H. Singer
Keyword(s):  
Electron Microscopy ◽  
Spatial Distribution ◽  
In Situ Hybridization ◽  
High Resolution ◽  
Nucleic Acids ◽  
Colloidal Gold ◽  
Dna Probe ◽  
Nucleotide Analogs ◽  
Gold Particles

We have been investigating the spatial distribution of nucleic acids intracellularly using in situ hybridization. The use of non-isotopic nucleotide analogs incorporated into the DNA probe allows the detection of the probe at its site of hybridization within the cell. This approach therefore is compatible with the high resolution available by electron microscopy. Biotinated or digoxigenated probe can be detected by antibodies conjugated to colloidal gold. Because mRNA serves as a template for the probe fragments, the colloidal gold particles are detected as arrays which allow it to be unequivocally distinguished from background.

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