scholarly journals Role of Chromosomal Passenger Complex in Chromosome Segregation and Cytokinesis

2001 ◽  
Vol 26 (6) ◽  
pp. 653-657 ◽  
Author(s):  
Yasuhiko Terada
Keyword(s):  
Chromosome Segregation ◽  
Chromosomal Passenger Complex ◽  
Chromosomal Passenger

scholarly journals Liquid-liquid phase separation of the chromosomal passenger complex drives parallel bundling of midzone microtubules

2022 ◽  
Author(s):  
Ewa Niedzialkowska ◽  
Tan M Truong ◽  
Luke A Eldredge ◽  
Stefanie Redemann ◽  
Denis Chretien ◽  
...  
Keyword(s):  
Phase Separation ◽  
Liquid Phase ◽  
Chromosome Segregation ◽  
Dynamic Structure ◽  
Liquid Phase Separation ◽  
Chromosomal Passenger Complex ◽  
Chromosomal Passenger ◽  
Spindle Midzone ◽  
Liquid Liquid Phase Separation

The spindle midzone is a dynamic structure that forms during anaphase, mediates chromosome segregation, and provides a signaling platform to position the cleavage furrow. The spindle midzone comprises two antiparallel bundles of microtubules (MTs) but the process of their formation is poorly understood. Here, we show that the Chromosomal Passenger Complex (CPC) undergoes liquid-liquid phase separation (LLPS) to generate parallel MT bundles in vitro when incubated with free tubulin and GTP. MT bundles emerge from CPC droplets with protruding minus-ends that then grow into long, tapered MT structures. During this growth, the CPC in condensates apparently reorganize to coat and bundle the resulting MT structures. CPC mutants attenuated for LLPS or MT binding prevented the generation of parallel MT bundles in vitro and reduced the number of MTs present at spindle midzones in HeLa cells. Our data uncovers a kinase-independent function of the CPC and provides models for how cells generate parallel-bundled MT structures that are important for the assembly of the mitotic spindle.

scholarly journals Kinesins relocalize the chromosomal passenger complex to the midzone for spindle disassembly

2018 ◽  
Vol 217 (5) ◽  
pp. 1687-1700 ◽  
Author(s):  
Itziar Ibarlucea-Benitez ◽  
Luke S. Ferro ◽  
David G. Drubin ◽  
Georjana Barnes
Keyword(s):  
Single Molecule ◽  
Molecular Mechanisms ◽  
Chromosomal Passenger Complex ◽  
Late Anaphase ◽  
Chromosomal Passenger ◽  
Spindle Disassembly ◽  
Spindle Midzone ◽  
Chromosome Separation

Mitotic spindle disassembly after chromosome separation is as important as spindle assembly, yet the molecular mechanisms for spindle disassembly are unclear. In this study, we investigated how the chromosomal passenger complex (CPC), which contains the Aurora B kinase Ipl1, swiftly concentrates at the spindle midzone in late anaphase, and we researched the role of this dramatic relocalization during spindle disassembly. We showed that the kinesins Kip1 and Kip3 are essential for CPC relocalization. In cells lacking Kip1 and Kip3, spindle disassembly is severely delayed until after contraction of the cytokinetic ring. Purified Kip1 and Kip3 interact directly with the CPC and recruit it to microtubules in vitro, and single-molecule experiments showed that the CPC diffuses dynamically on microtubules but that diffusion stops when the CPC encounters a Kip1 molecule. We propose that Kip1 and Kip3 trap the CPC at the spindle midzone in late anaphase to ensure timely spindle disassembly.

scholarly journals The chromosomal passenger complex establishes chromosome biorientation via two parallel localization pathways

2020 ◽  
Author(s):  
Theodor Marsoner ◽  
Poornima Yedavalli ◽  
Chiara Masnovo ◽  
Katrin Schmitzer ◽  
Christopher S. Campbell
Keyword(s):  
Chromosome Segregation ◽  
Budding Yeast ◽  
Small Region ◽  
The Other ◽  
Chromosomal Passenger Complex ◽  
Microtubule Binding ◽  
Chromosome Alignment ◽  
Chromosomal Passenger ◽  
Spindle Microtubules ◽  
Do So

AbstractChromosome biorientation is established by the four-member chromosomal passenger complex (CPC) through phosphorylation of incorrect kinetochore-microtubule attachments. During chromosome alignment, the CPC localizes to the inner centromere, the inner kinetochore and spindle microtubules. Here we show that a small region of the CPC subunit INCENP/Sli15 is required to target the complex to all three of these locations in budding yeast. This region, the SAH, is essential for phosphorylation of outer kinetochore substrates, chromosome segregation, and viability. By restoring the CPC to each of these three locations individually, we found that inner centromere localization is sufficient to establish chromosome biorientation and viability independently of the other two targeting mechanisms. Remarkably, although neither the inner kinetochore nor microtubule binding activities was able to rescue viability individually, they were able to do so when combined. We have therefore identified two parallel pathways by which the CPC can promote chromosome biorientation and proper completion of mitosis.

Phosphorylation of histone H3 by Haspin regulates chromosome alignment and segregation during mitosis in maize

2020 ◽  
Author(s):  
Yang Liu ◽  
Chunhui Wang ◽  
Handong Su ◽  
James A Birchler ◽  
Fangpu Han
Keyword(s):  
Chromosome Segregation ◽  
Histone H3 ◽  
Heterochromatin Protein 1 ◽  
Heterochromatin Protein ◽  
Factor B ◽  
Microtubule Attachment ◽  
Chromosome Alignment ◽  
Chromosomal Passenger ◽  
Mitosis And Meiosis

Abstract In human cells, Haspin-mediated histone H3 threonine 3 (H3T3) phosphorylation promotes centromeric localization of the chromosomal passenger complex, thereby ensuring proper kinetochore–microtubule attachment. Haspin also binds to PDS5 cohesin-associated factor B (Pds5B), antagonizing the Wings apart-like protein homolog (Wapl)–Pds5B interaction and thus preventing Wapl from releasing centromeric cohesion during mitosis. However, the role of Haspin in plant chromosome segregation is not well understood. Here, we show that in maize (Zea mays) mitotic cells, ZmHaspin localized to the centromere during metaphase and anaphase, whereas it localized to the telomeres during meiosis. These results suggest that ZmHaspin plays different roles during mitosis and meiosis. Knockout of ZmHaspin led to decreased H3T3 phosphorylation and histone H3 serine 10 phosphorylation, and defects in chromosome alignment and segregation in mitosis. These lines of evidence suggest that Haspin regulates chromosome segregation in plants via the mechanism described for humans, namely, H3T3 phosphorylation. Plant Haspin proteins lack the RTYGA and PxVxL motifs needed to bind Pds5B and heterochromatin protein 1, and no obvious cohesion defects were detected in ZmHaspin knockout plants. Taken together, these results highlight the conserved but slightly different roles of Haspin proteins in cell division in plants and in animals.

scholarly journals Borealin–nucleosome interaction secures chromosome association of the chromosomal passenger complex

2019 ◽  
Vol 218 (12) ◽  
pp. 3912-3925 ◽  
Author(s):  
Maria A. Abad ◽  
Jan G. Ruppert ◽  
Lana Buzuk ◽  
Martin Wear ◽  
Juan Zou ◽  
...  
Keyword(s):  
Cell Division ◽  
Chromosome Segregation ◽  
Chromosome Association ◽  
Aurora B ◽  
Master Regulator ◽  
Multifaceted Approach ◽  
Chromosomal Passenger Complex ◽  
Chromosome Congression ◽  
Chromosomal Passenger ◽  
And Function

Chromosome association of the chromosomal passenger complex (CPC; consisting of Borealin, Survivin, INCENP, and the Aurora B kinase) is essential to achieve error-free chromosome segregation during cell division. Hence, understanding the mechanisms driving the chromosome association of the CPC is of paramount importance. Here using a multifaceted approach, we show that the CPC binds nucleosomes through a multivalent interaction predominantly involving Borealin. Strikingly, Survivin, previously suggested to target the CPC to centromeres, failed to bind nucleosomes on its own and requires Borealin and INCENP for its binding. Disrupting Borealin–nucleosome interactions excluded the CPC from chromosomes and caused chromosome congression defects. We also show that Borealin-mediated chromosome association of the CPC is critical for Haspin- and Bub1-mediated centromere enrichment of the CPC and works upstream of the latter. Our work thus establishes Borealin as a master regulator determining the chromosome association and function of the CPC.

scholarly journals The Borealin dimerization domain interacts with Sgo1 to drive Aurora B–mediated spindle assembly

2020 ◽  
Vol 31 (20) ◽  
pp. 2207-2218 ◽  
Author(s):  
Mary Kate Bonner ◽  
Julian Haase ◽  
Hayden Saunders ◽  
Hindol Gupta ◽  
Biyun Iris Li ◽  
...  
Keyword(s):  
Molecular Mechanism ◽  
Spindle Assembly ◽  
Specific Role ◽  
Aurora B ◽  
Dimerization Domain ◽  
Chromosomal Passenger Complex ◽  
Chromosomal Passenger ◽  
Segregation Of Chromosomes

This study provides the molecular mechanism for the interaction of Sgo1 with the chromosomal passenger complex and explores the specific role of Sgo1 in regulating Aurora B functions that ensure the equal segregation of chromosomes.

Cytokinesis: the final stop for the chromosomal passengers

2008 ◽  
Vol 36 (3) ◽  
pp. 367-370 ◽  
Author(s):  
Mar Carmena
Keyword(s):  
Chromosomal Passenger Complex ◽  
Chromosomal Passenger ◽  
Functional Analyses ◽  
Mitosis And Meiosis ◽  
Late Stages

The CPC (chromosomal passenger complex) performs essential roles in the regulation and co-ordination of chromosomal and cytoskeletal events during mitosis and meiosis. The first functional analyses showed evidence of a role of the CPC in the regulation of cytokinesis. In this review, I summarize what we have learned since then about the role of the CPC in the late stages of mitosis and cytokinesis.

scholarly journals Chromosome segregation regulation in human zygotes: altered mitotic histone phosphorylation dynamics underlying centromeric targeting of the chromosomal passenger complex

2015 ◽  
Vol 30 (10) ◽  
pp. 2275-2291 ◽  
Author(s):  
C. van de Werken ◽  
M. Avo Santos ◽  
J.S.E. Laven ◽  
C. Eleveld ◽  
B.C.J.M. Fauser ◽  
...  
Keyword(s):  
Chromosome Segregation ◽  
Chromosomal Passenger Complex ◽  
Histone Phosphorylation ◽  
Chromosomal Passenger ◽  
Human Zygotes

Functionality of the chromosomal passenger complex in cancer

2015 ◽  
Vol 43 (1) ◽  
pp. 23-32 ◽  
Author(s):  
Sanne Hindriksen ◽  
Amanda Meppelink ◽  
Susanne M.A. Lens
Keyword(s):  
Cell Division ◽  
Chromosome Segregation ◽  
Chromosomal Instability ◽  
Cultured Cells ◽  
Present Review ◽  
Next Generation ◽  
Chromosomal Passenger Complex ◽  
Human Cancers ◽  
Chromosomal Passenger ◽  
Ploidy Status

The evolutionary conserved chromosomal passenger complex (CPC) is essential for faithful transmission of the genome during cell division. Perturbation of this complex in cultured cells gives rise to chromosome segregation errors and cytokinesis failure and as a consequence the ploidy status of the next generation of cells is changed. Aneuploidy and chromosomal instability (CIN) is observed in many human cancers, but whether this may be caused by deregulation of the CPC is unknown. In the present review, we discuss if and how a dysfunctional CPC could contribute to CIN in cancer.

Sign in / Sign up

Export Citation Format

Share Document