Transposon-induced epigenetic silencing in the X chromosome as a novel form of dmrt1 expression regulation during sex determination in the fighting fish

Background Fishes are the one of the most diverse groups of animals with respect to their modes of sex determination, providing unique models for uncovering the evolutionary and molecular mechanisms underlying sex determination and reversal. Here, we have investigated how sex is determined in a species of both commercial and ecological importance, the Siamese fighting fish Betta splendens. Results We conducted association mapping on four commercial and two wild populations of B. splendens. In three of the four commercial populations, the master sex determining (MSD) locus was found to be located in a region of ~ 80 kb on LG2 which harbours five protein coding genes, including dmrt1, a gene involved in male sex determination in different animal taxa. In these fish, dmrt1 shows a male-biased gonadal expression from undifferentiated stages to adult organs and the knockout of this gene resulted in ovarian development in XY genotypes. Genome sequencing of XX and YY genotypes identified a transposon, drbx1, inserted into the fourth intron of the X-linked dmrt1 allele. Methylation assays revealed that epigenetic changes induced by drbx1 spread out to the promoter region of dmrt1. In addition, drbx1 being inserted between two closely linked cis-regulatory elements reduced their enhancer activities. Thus, epigenetic changes, induced by drbx1, contribute to the reduced expression of the X-linked dmrt1 allele, leading to female development. This represents a previously undescribed solution in animals relying on dmrt1 function for sex determination. Differentiation between the X and Y chromosomes is limited to a small region of ~ 200 kb surrounding the MSD gene. Recombination suppression spread slightly out of the SD locus. However, this mechanism was not found in the fourth commercial stock we studied, or in the two wild populations analysed, suggesting that it originated recently during domestication. Conclusions Taken together, our data provide novel insights into the role of epigenetic regulation of dmrt1 in sex determination and turnover of SD systems and suggest that fighting fish are a suitable model to study the initial stages of sex chromosome evolution.

A New Moving Kirchhoff-Love Plate Element for Dynamic Analysis of Vehicle-Pavement Interaction

A new moving Kirchhoff-Love plate element is developed in this work to accurately and efficiently calculate the dynamic response of vehicle-pavement interaction. Since the vehicle can only affect a small region nearby, the wide pavement is reduced to a small reduced plate area around the vehicle. The vehicle loads moving along an arbitrary trajectory is considered, and the arbitrary Lagrangian-Eulerian method is used here for coordinate conversion. The reduced plate area is spatially discretized using the current moving plate element, where its governing equations are derived using Lagrange's equations. The moving plate element is validated by different plate subjected to moving load cases, where the influences of different factors on reduced plate area length of the RBM model are also investigated. Then a vehicle-pavement interaction case with constant and variable speed is analyzed here. The calculation results from the moving plate element are in good agreement with those from the modal superposition method (MSM), and the calculation time with the moving plate element is only one third of that using the MSM. It is also found that the moving load velocity and ground damping have great influences on reduced plate area length of the RBM. The moving plate element is accurate and more efficient than the MSM in calculating the dynamic response of the vehicle-pavement interaction.

Ramp-shaped neural tuning supports graded population-level representation of the object-to-scene continuum

We can easily perceive the spatial scale depicted in a picture, regardless of whether it is a small space (e.g., a close-up view of a chair) or a much larger space (e.g., an entire class room). How does the human visual system encode this continuous dimension? Here, we investigated the underlying neural coding of depicted spatial scale, by examining the voxel tuning and topographic organization of brain responses. We created naturalistic yet carefully-controlled stimuli by constructing virtual indoor environments, and rendered a series of snapshots to smoothly sample between a close-up view of the central object and far-scale view of the full environment (object-to-scene continuum). Human brain responses were measured to each position using functional magnetic resonance imaging. We did not find evidence for a smooth topographic mapping for the object-to-scene continuum on the cortex. Instead, we observed large swaths of cortex with opposing ramp-shaped profiles, with highest responses to one end of the object-to-scene continuum or the other, and a small region showing a weak tuning to intermediate scale views. Importantly, when we considered the multi-voxel patterns of the entire ventral occipito-temporal cortex, we found smooth and linear representation of the object-to-scene continuum. Thus, our results together suggest that depicted spatial scale is coded parametrically in large-scale population codes across the entire ventral occipito-temporal cortex.

Isolation and characterization of α ‐amylase encoding gene in Bacillus amyloliquefaciens PAS

Amylolytic bacteria are a source of amylase, which is an essential enzyme to support microalgae growth in the bioreactor for microalgae culture. In a previous study, the highest bacterial isolate to hydrolyze amylum (namely PAS) was successfully isolated from Ranu Pani, Indonesia, and it was identified as Bacillus amyloliquefaciens. That bacterial isolate (B. amyloliquefaciens PAS) also has been proven to accelerate Chlorella vulgaris growth in the mini bioreactor. This study aims to detect, isolate, and characterize the PAS’s α‐amylase encoding gene. This study was conducted with DNA extraction, amplification of α‐amylase gene with polymerase chain reaction (PCR) method with the specific primers, DNA sequencing, phylogenetic tree construction, and protein modeling. The result showed that α‐amylase was successfully detected in PAS bacterial isolate. The α‐amylase DNA fragment was obtained 1,468 bp and that translated sequence has an identity of about 98.3% compared to the B. amylolyquefaciens α‐amylase 3BH4 in the Protein Data Bank (PDB). The predicted 3D protein model of the PAS’s α‐amylase encoding gene has amino acid variations that predicted affect the protein’s structure in the small region. This research will be useful for further research to produce recombinant α‐amylase.

Dynamic Relationship Study between the Observed Seismicity and Spatiotemporal Pattern of Lineament Changes in Palghar, North Maharashtra (India)

The Palghar region (north Maharashtra, India), located in the northwestern part of the stable continental region of India, experienced a low magnitude earthquake swarm, which was initiated in September 2018 and is continuing to date (as of October 2021). From December 2018 to December 2020, ~5000 earthquakes with magnitudes from M1.2 to M3.8 occurred in a small region of 20 × 10 km2. These earthquakes were probably triggered by fluid migration during seasonal rainfall. In this study, we have used multi-temporal Landsat satellite data of the year 2000, 2015, 2018, 2019, and 2020, extracted lineaments, and studied the changes in frequency and pattern of lineaments before and after the initiation of the swarm in the Palghar region. An increase in the lineament density and amount of rainfall are found to be associated with the increasing frequency of earthquakes.

Underwater gas self-transportation along the femtosecond laser-written open superhydrophobic surface microchannels (< 100 µm) for bubble/gas manipulation

Underwater transportation of bubbles and gases has essential applications in manipulating and using gas, but there is still a great challenge to achieve this function at the microscopic level. Here, we report a strategy to self-transport gas along the laser-induced open superhydrophobic microchannel with a width less than 100 µm in water. The femtosecond laser can directly write superhydrophobic and underwater superaerophilic microgrooves on the polytetrafluoroethylene (PTFE) surface. In water, the single laser-induced microgroove and water medium generate a hollow microchannel. When the microchannel connects two superhydrophobic regions in water, the gas can be spontaneously transported from the small region to the large area along this hollow microchannel. The gas self-transportation can be extended to the laser-drilled microholes through a thin PTFE sheet. Anti-buoyancy unidirectional penetration is even achieved. The gas can overcome the buoyance of the bubble and spontaneously transport downward. The Laplace pressure difference drives the processes of spontaneous gas transportation and unidirectional bubble passage. We believe the property of gas self-transportation in the femtosecond laser-structured open superhydrophobic and underwater superaerophilic microgrooves/microholes has significant potential applications related to manipulating underwater gas.

Self-confined expression in the Arabidopsis root stem cell niche

Stem cell niches are local microenvironments that preserve their unique identity while communicating with adjacent tissues. In the primary root of Arabidopsis thaliana, the stem cell niche comprises the expression of two transcription factors, BRAVO and WOX5, among others. Intriguingly, these proteins confine their own gene expression to the niche, as evidenced in each mutant background. Here we propose through mathematical modeling that BRAVO confines its own expression domain to the stem cell niche by attenuating its WOX5-dependent diffusible activator. This negative feedback drives WOX5 action to be spatially restricted as well. The results show that WOX5 diffusion and sequestration by binding to BRAVO is sufficient to drive realistic confined BRAVO expression at the stem cell niche. We propose that attenuation of a diffusible activator can be a general mechanism to confine genetic activity to a small region while at the same time maintain signaling within it and with the surrounding cells.

Influence of Print Settings on the Critical Quality Attributes of Extrusion-Based 3D-Printed Caplets: A Quality-by-Design Approach

Extrusion-based 3D-printing is an easy-to-use, cheap manufacturing technique that could be used to produce tailored precision medicines. The technique has an almost unlimited versatility since a multitude of print parameters can easily be adapted. Unfortunately, little is known of the effect of these print parameters on the critical quality attributes of the resulting printlets. In this study, practical guidelines and means to adapt certain parameters in order to achieve the desired outcome (e.g., acceptable visual quality and flexible dosing) are stipulated for medical 3D-printing using a design-of-experiments approach. The current study aims at elucidating the effect of five print parameters (infill, overlap, number of shells, layer height and layer pattern) on the mechanical properties, dimensions, weight, porosity and dissolution characteristics of a fixed-size caplet consisting of Eudragit EPO (69.3%), Polyox WSR N10 (29.7%) and zolpidem hemitartrate (1%). In terms of the mechanical properties, 3D-printed caplets possessed anisotropy where the vertical compression strength and Brinell hardness exceeded the diametral strength. In general, all 3D-printed caplets possessed acceptable mechanical strength except for a small region of the knowledge space. Dimensional analysis revealed small, statistical significant differences between different runs, although the clinical relevance of this variation is likely negligible. The weight or dose of a caplet can be varied mainly using the infill and overlap and, to a lesser extent, via the layer height and number of shells. The impact on porosity was complicated as this was influenced by many factors and their interactions. Infill was the only statistically relevant factor influencing the dissolution rate of the current formulation. This study unravels the importance of the print parameter overlap, which is a regularly neglected parameter. We also discovered that small dose variations while maintaining the same dissolution profile were possible via modifying the overlap or number of shells. However, large dose variations without affecting the dissolution behaviour could only be accomplished by size modifications of the printlet.

V. A. Moshkov: the birth of gagauz studies in Russia

The Russian ethnographer V. A. Moshkov was a general in rank and a member of the Russian Geographical Society and seriously studied the traditions and life of the peoples inhabiting the Russian Empire. At the end of the 19th century, he began to research the Gagauz people. The research “The Gagauz of Bendery Uyezd”, published in the journal “Ethnographic Review” at the beginning of the 20th century was published as a result of V. A. Moshkov’s travels. Thanks to V. A. Moshkov, the general public learned about the small Orthodox people living on the area of a small region in the south of Bessarabia – the Gagauz. This article examines the multifaceted activities of the researcher aimed at highlighting various aspects of traditional culture – material culture, as well as customs, rituals, beliefs and folklore. It is noted that V. A. Moshkov tried to study the problem of human origin. It is curious that he was prompted to consider this issue because of his acquaintance with the material and spiritual culture of the Gagauz people, in which the scientist found parallels with other peoples not related to the Gagauz people by a common origin. The authors emphasize the role of the Gagauz scholars who have devoted their attention to studying the scientific heritage of V. A. Moshkov in the preservation and development of the identity of the Gagauz ethnos.

Forced Linear Shear Flows with Rotation: Rotating Couette–Poiseuille Flow, Its Stability, and Astrophysical Implications

We explore the effect of forcing on the linear shear flow or plane Couette flow, which is also the background flow in the very small region of the Keplerian accretion disk. We show that depending on the strength of forcing and boundary conditions suitable for the systems under consideration, the background plane shear flow, and hence the accretion disk velocity profile, is modified into parabolic flow, which is a plane Poiseuille flow or Couette–Poiseuille flow, depending on the frame of reference. In the presence of rotation, the plane Poiseuille flow becomes unstable at a smaller Reynolds number under pure vertical as well as three-dimensional perturbations. Hence, while rotation stabilizes the plane Couette flow, the same destabilizes the plane Poiseuille flow faster and hence the forced local accretion disk. Depending on the various factors, when the local linear shear flow becomes a Poiseuille flow in the shearing box due to the presence of extra force, the flow becomes unstable even for Keplerian rotation, and hence turbulence will ensue. This helps to resolve the long-standing problem of subcritical transition to turbulence in hydrodynamic accretion disks and the laboratory plane Couette flow.