scholarly journals Urinary Excretion of Thiobarbituric Acid Reactive Substances of Healthy Subjects Supplemented with a High Dose of d-.ALPHA.-Tocopherol.

1995 ◽  
Vol 18 (9) ◽  
pp. 1275-1278 ◽  
Author(s):  
Hiroko KOSUGI ◽  
Yoshiko ASANO ◽  
Tomio NAGAYAMA ◽  
Masatoshi BEPPU ◽  
Kiyomi KIKUGAWA
Keyword(s):  
Urinary Excretion ◽  
Healthy Subjects ◽  
Thiobarbituric Acid ◽  
Alpha Tocopherol ◽  
High Dose ◽  
Thiobarbituric Acid Reactive Substances

Dehydroepiandrosterone administration prevents the oxidative damage induced by acute hyperglycemia in rats

1997 ◽  
Vol 155 (2) ◽  
pp. 233-240 ◽  
Author(s):  
M Aragno ◽  
E Brignardello ◽  
E Tamagno ◽  
V Gatto ◽  
O Danni ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Properties ◽  
Thiobarbituric Acid ◽  
Alpha Tocopherol ◽  
Thiobarbituric Acid Reactive Substances ◽  
Acute Hyperglycemia ◽  
Tissue Resistance ◽  
In Vivo Administration ◽  
Different Doses

Free radical overproduction contributes to tissue damage induced by acute hyperglycemia. Dehydroepiandrosterone, which has recently been found to have antioxidant properties, was administered i.p. to rats at different doses (10, 50 or 100 mg/kg body weight) 3 h before treatment with dextrose (5 g/kg). Lipid peroxidation was evaluated on liver, brain and kidney homogenates, measuring both steady-state concentrations of thiobarbituric acid reactive substances, and fluorescent chromolipids, evaluated as hydroxynonenal adducts. Formation of thiobarbituric acid reactive substances was significantly higher in hyperglycemic than in normoglycemic animals. Three hours (but not 1 h) dehydroepiandrosterone-pretreatment protected tissues against lipid peroxidation induced by dextrose; both thiobarbituric acid reactive substances and hydroxynonenal adducts in liver, kidney and brain homogenates were significantly lower in dehydroepiandrosterone-pretreated animals. Dehydroepiandrosterone did not modify the cytosolic level of antioxidants, such as alpha-tocopherol or glutathione, nor the activities of glutathione peroxidase, reductase or transferase. The results of this study indicate that the 'in vivo' administration of dehydroepiandrosterone increases tissue resistance to lipid peroxidation triggered by acute hyperglycemia.

scholarly journals High-dose testosterone supplementation disturbs liver pro-oxidant/antioxidant balance and function in adolescent male Wistar rats undergoing moderate-intensity endurance training

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e10228
Author(s):  
Ewa Sadowska-Krępa ◽  
Barbara Kłapcińska ◽  
Anna Nowara ◽  
Sławomir Jagsz ◽  
Izabela Szołtysek-Bołdys ◽  
...  
Keyword(s):  
Endurance Training ◽  
Low Dose ◽  
Thiobarbituric Acid ◽  
Adolescent Male ◽  
Moderate Intensity ◽  
High Dose ◽  
Anabolic Androgenic Steroid ◽  
Thiobarbituric Acid Reactive Substances ◽  
Androgenic Steroid ◽  
Male Wistar Rats

In some countries, anabolic-androgenic steroid abuse is rampant among adolescent boys and young men, including some of those seeking physical fitness and/or pleasing appearance through various exercise types. This tactic carries the risk of severe harmful health effects, including liver injury. Most anabolic-androgenic steroid stacking protocols employed are based on the use of the ‘prototypic’ anabolic-androgenic steroid testosterone and/or its esters. There is a vast body of data on the effects of anabolic-androgenic steroids’ abuse combined with physical exercise training on the liver antioxidant barrier in adult subjects, whereas those concerning adolescents are scant. This study aimed to assess, in adolescent male Wistar rats undergoing a 6-week moderate-intensity endurance training (treadmill running), the influence of concurrent weekly supplementation with intramuscular testosterone enanthate (TE, 8 or 80 mg/kg body weight/week) on selected indices of liver status and oxidative stress. The rats were sacrificed, and their livers and blood samples were harvested two days after the last training session. High-dose TE treatment significantly reduced body and liver weight gains. Neither low-dose nor high-dose TE treatment affected liver α-tocopherol or γ-tocopherol content, whereas low-dose TE treatment significantly lowered hepatic reduced glutathione content. TE treatment significantly elevated liver thiobarbituric acid-reactive substances content and blood activities of alkaline phosphatase and γ-glutamyltransferase, but not of aspartate aminotransferase or alanine aminotransferase. Liver catalase activity was lowered by >50% in both TE-treated groups, while superoxide dismutase activity was significantly but slightly affected (−15%) only by the high-dose TE treatment. Glutathione peroxidase and glutathione reductase activities were not significantly altered. TE treatment significantly increased liver thiobarbituric acid-reactive substances content and lowered blood HDL-cholesterol, but did not significantly affect LDL-cholesterol or triglycerides level. In conclusion, high-dose TE treatment significantly disturbed liver antioxidant barrier and prooxidative-antioxidative balance and hence counteracted favorable effects of concurrent moderate-intensity endurance training in adolescent male rats.

scholarly journals Meat quality of lambs supplemented with intramuscular vitamin E

2018 ◽  
Vol 38 (4) ◽  
pp. 679-684
Author(s):  
Aline A. Morgado ◽  
Giovanna R. Nunes ◽  
Beatriz R. Villas Bôas ◽  
Paola B.J. Carvalho ◽  
Paulo H.M. Rodrigues ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Vitamin E ◽  
Meat Quality ◽  
Live Weight ◽  
Thiobarbituric Acid ◽  
Alpha Tocopherol ◽  
Thiobarbituric Acid Reactive Substances ◽  
Muscle Ph ◽  
Four Blocks

ABSTRACT: This study evaluated the effects of intramuscular alpha-tocopherol (vitamin E) supplementation on meat quality characteristics of Santa Inês and Dorper crossbreed lambs. All animals were feed with a high concentrated diet in feedlot. Eight days before slaughter, the animals were distributed into four blocks according to weight gain. At the seventh and fourth days before slaughter, they were intramuscularly treated with 0, 10 or 20 IU of DL-alpha-tocopherol per kg of metabolic body weight. At slaughter they had 138 days of age and 43.6 kg of live weight, in average. Carcasses were stored for 24 hours under refrigeration at 2°C. Longissimus thoracis muscle pH (pH24h) and color (lightness, yellowness and redness) were analyzed and its samples were collected for evaluation of shear force (SF), cooking loss (WLC), fatty acid composition (FA) and thiobarbituric acid reactive substances after one (TBARS1m) and after five months (TBARS5m) of freezing. Linearity deviation effect was observed for lightness (L*; P=0.0042) and yellowness (b*; P=0.0082). Intramuscular administration of 10 or 20 IU of alpha-tocopherol/kg of metabolic weight did not influence the conservation of fatty acid in the carcasses, but benefit L* and b* values.

scholarly journals Liver and serum antioxidant status after methanol intoxication in rats.

1997 ◽  
Vol 44 (1) ◽  
pp. 139-145 ◽  
Author(s):  
E Skrzydlewska ◽  
R Farbiszewski
Keyword(s):  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Antioxidant Status ◽  
Thiobarbituric Acid ◽  
Alpha Tocopherol ◽  
Thiobarbituric Acid Reactive Substances ◽  
Methanol Intoxication ◽  
Sh Groups ◽  
Sulfhydryl Compounds ◽  
Antioxidant Mechanisms

Activities of superoxide dismutase (SOD), catalase, glutathione peroxidase (GSH-Px) and glutathione reductase (GSSG-R) and concentration of ascorbate, alpha-tocopherol, non-protein and protein-bound sulfhydryl compounds and thiobarbituric acid-reactive substances (TBA-rs) were measured in liver and serum of rats 6, 12 and 24 h and 2, 5 and 7 days after intoxication with 1.5 g or 3.0 g methanol/kg b.w. Liver GSH-Px and GSSG-R activities and SH-groups and ascorbate content were significantly diminished at 6 and 24 h, while TBA-rs were increased. Serum SOD, GSH-Px and GSSG-R activities and SH-groups concentration were reduced while TBA-rs were elevated. The changes were more intensive after application of the higher dose of methanol. It is concluded that methanol impairs the liver and blood serum antioxidant mechanisms in rats.

scholarly journals Circulating lipid peroxidation, plasma and erythrocyte antioxidant status in patients with rheumatoid arthritis

2009 ◽  
Vol 35 (2) ◽  
pp. 57-62 ◽  
Author(s):  
Palanisamy Pasupathi ◽  
Mathiyalagan Deepa ◽  
P. Rani ◽  
R. Ramesh Sankar
Keyword(s):  
Rheumatoid Arthritis ◽  
Lipid Peroxidation ◽  
Healthy Subjects ◽  
Antioxidant Defense ◽  
Defense Mechanism ◽  
Thiobarbituric Acid ◽  
Antioxidant Defense System ◽  
Thiobarbituric Acid Reactive Substances ◽  
Reactive Protein ◽  
Species Production

The aim of the study was to investigate the levels of lipid peroxidation, and both plasma and erythrocyte antioxidant states in patients with rheumatoid arthritis. The population consisted of 60 subjects divided into two groups, 30 subjects had evidence of rheumatoid arthritis and age and sex matched healthy subjects were studied as controls. The level of plasma and erythrocyte thiobarbituric acid reactive substances (TBARS) was markedly increased in both the rheumatoid arthritis patients when compared to control subjects. The activities of plasma and erythrocyte antioxidants were significantly decreased in rheumatoid arthritis. C reactive protein, rheumatoid factor and antistreptolysin-O were significantly higher in rheumatoid arthritis patients than in healthy subjects. In conclusion, on the basis of enhanced lipid peroxidation in rheumatoid arthritis patients with concomitant failure of both the plasma, and erythrocyte antioxidants defense mechanism. These results are consistent with the underlying hypothesis that there is an  imbalance between reactive oxygen species production and the antioxidant defense system in inflammatory rheumatoid arthritis disease.Keywords: Antioxidant; Lipid peroxidation; Rheumatoid arthritisOnline: 13 August 2009DOI: 10.3329/bmrcb.v35i2.2798Bangladesh Med Res Counc Bull 2009; 35: 57-62

Exhalation of H2O2 and thiobarbituric acid reactive substances (TBARs) by healthy subjects

2001 ◽  
Vol 30 (2) ◽  
pp. 178-186 ◽  
Author(s):  
Dariusz Nowak ◽  
Sylwia Kałucka ◽  
Piotr Białasiewicz ◽  
Maciej Król
Keyword(s):  
Healthy Subjects ◽  
Thiobarbituric Acid ◽  
Thiobarbituric Acid Reactive Substances
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