Specific Hybrid Liposomes Composed of Phosphatidylcholine and Polyoxyethylenealkyl Ether with Markedly Enhanced Inhibitory Effects on the Growth of Tumor Cells In Vitro.

Canine oral mucosal melanomas (OMM) are the most common oral malignancy in dogs and few treatments are available. Thus, new treatment modalities are needed for this disease. Bacillus anthracis (anthrax) toxin has been reengineered to target tumor cells that express urokinase plasminogen activator (uPA) and metalloproteinases (MMP-2), and has shown antineoplastic effects both, in vitro and in vivo. This study aimed to evaluate the effects of a reengineered anthrax toxin on canine OMM. Five dogs bearing OMM without lung metastasis were included in the clinical study. Tumor tissue was analyzed by immunohistochemistry for expression of uPA, uPA receptor, MMP-2, MT1-MMP and TIMP-2. Animals received either three or six intratumoral injections of the reengineered anthrax toxin prior to surgical tumor excision. OMM samples from the five dogs were positive for all antibodies. After intratumoral treatment, all dogs showed stable disease according to the canine Response Evaluation Criteria in Solid Tumors (cRECIST), and tumors had decreased bleeding. Histopathology has shown necrosis of tumor cells and blood vessel walls after treatment. No significant systemic side effects were noted. In conclusion, the reengineered anthrax toxin exerted inhibitory effects when administered intratumorally, and systemic administration of this toxin is a promising therapy for canine OMM.

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Waste Treatment Technologies. Basic Study on New Systems for Converting Shochu Distillation Remnants into Useful Materials: Inhibitory Effects on the Growth of Tumor Cells.

KAGAKU KOGAKU RONBUNSHU ◽

10.1252/kakoronbunshu.28.621 ◽

2002 ◽

Vol 28 (5) ◽

pp. 621-625 ◽

Cited By ~ 9

Author(s):

Shigeru Hirose ◽

Osamu Tanoue ◽

Haruyuki Iefuji ◽

Yoko Matsumoto ◽

Ryuichi Ueoka

Keyword(s):

Tumor Cells ◽

Waste Treatment ◽

Inhibitory Effects ◽

Basic Study ◽

Treatment Technologies ◽

Growth Of Tumor

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In vitro and in vivo biological characterization of the anti-proliferative potential of a cyclic trinuclear organotin(iv) complex

Molecular BioSystems ◽

10.1039/c5mb00791g ◽

2016 ◽

Vol 12 (3) ◽

pp. 1015-1023 ◽

Cited By ~ 12

Author(s):

Marta Martins ◽

Pedro V. Baptista ◽

Ana Soraia Mendo ◽

Claudia Correia ◽

Paula Videira ◽

...

Keyword(s):

Tumor Cells ◽

Proliferative Potential ◽

Biological Characterization ◽

Growth Of Tumor

Identification of novel molecules that can selectively inhibit the growth of tumor cells, is of utmost importance.

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Programming the anti-tumor immune response in vitro and its application to stop the growth of tumor cells and prolonging the lifespan of mice with carcinoma in vivo

ZHurnal «Patologicheskaia fiziologiia i eksperimental`naia terapiia» ◽

10.25557/igpp.2017.4.8525 ◽

2017 ◽

pp. 67-73

Author(s):

С.В. Калиш ◽

С.В. Лямина ◽

А.А. Раецкая ◽

О.П. Буданова ◽

И.Ю. Малышев

Keyword(s):

Immune Response ◽

Tumor Growth ◽

Tumor Cells ◽

The Body ◽

Ehrlich Carcinoma ◽

Ec Cells ◽

Growth Of Tumor ◽

Immune Response In Vitro

Цель - представить доказательства правомерности гипотезы, что комбинированный пул репрограммированных in vitro макрофагов и лимфоцитов будет эффективно ограничивать пролиферацию опухолевых клеток in vitro , а при введении в организм будет существенно ограничивать развитие опухоли in vivo . Методика. Размножение опухолевых клеток инициировали in vitro путем добавления клеток карциномы Эрлиха (КЭ) в среду культивирования RPMI-1640. Развитие асцитной опухоли in vivo воспроизводили путем внутрибрюшной инъекции клеток КЭ мышам. Результаты. Установлено, что M3 макрофаги вместе с антиген-репрограммированными лимфоцитами оказывают выраженный противоопухолевый эффект и in vitro, и in vivo , который был существеннее противоопухолевого эффекта цисплатина. Заключение. Факты, свидетельствующие, что М3 макрофаги в сочетании с in vitro антиген-репрограммированными лимфоцитами значительно подавляют рост опухоли in vivo , делают перспективным разработку клинической версии биотехнологии ограничения роста опухоли путем предварительного программирования противоопухолевого иммунного ответа «в пробирке». Aim. To test a hypothesis that a combined pool of in vitro reprogrammed macrophages and lymphocytes will effectively limit growth of tumor cells in vitro , and injections of these cells into the body will considerably limit development of a tumor in vivo . Methods. Tumor growth was initiated in vitro by addition of Ehrlich carcinoma (EC) cells to the RPMI-1640 cell culture medium and in vivo by intraperitoneal injection of EC cells into mice. Results. M3 macrophages in combination with antigen-reprogrammed lymphocytes exerted a pronounced antitumor effect both in vitro and in vivo, which was superior to the effect of cisplatin. Conclusion. M3 macrophages in combination with in vitro antigen-reprogrammed lymphocytes significantly inhibited the tumor growth in vivo . This fact justifies development of a clinical version of the tumor growth restricting biotechnology using pre-programming of the antitumor immune response in vitro .

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