scholarly journals Activity-Guided Fractionation of Green Tea Extract with Antiproliferative Activity against Human Stomach Cancer Cells

2002 ◽  
Vol 25 (9) ◽  
pp. 1238-1240 ◽  
Author(s):  
Junei Kinjo ◽  
Tsuneatsu Nagao ◽  
Takashi Tanaka ◽  
Gen-ichiro Nonaka ◽  
Masafumi Okawa ◽  
...  
2020 ◽  
Vol 6 (2) ◽  
Author(s):  
Lisni Noraida Waruwu ◽  
Maria Bintang ◽  
Bambang Pontjo Priosoeryanto

Green tea (Camellia sinensis) is one of traditional plants that have the potential as an anticancer. The sample used in this research commercial green tea extract. The purpose of this study was to test the antiproliferation activity of green tea extract on breast cancer cell MCM-B2 in vitro. Green tea extract fractionated using three solvents, ie water, ethanol 70%, and n-hexane. Extract and fraction of green tea water have value Lethality Concentration 50 (LC50) more than 1000 ppm. The fraction of ethanol 70% and n-hexane had an LC50 value of 883.48 ppm and 600.56 ppm, respectively. The results of the phytochemical screening of green tea extract are flavonoids, tannins, and saponins, while the phytochemical screening results of n-hexane fraction are flavonoids and tannins. Antiproliferation activity was tested on breast cancer cells MCM-B2 and normal cells Vero by trypan blue staining method. The highest MCM-B2 cell inhibitory activity was achieved at a concentration of 13000 ppm green tea extract and 1000 ppm of n-hexane fraction, 59% and 59%, respectively. The extract and n-hexane fraction of green tea are not toxic to normal Vero cells characterized by not inhibiting normal cell proliferation. Keywords: antiproliferative, cancer cell MCM-B2, commercial green tea, cytotoxicity


1999 ◽  
Vol 90 (7) ◽  
pp. 733-739 ◽  
Author(s):  
Sachiko Okabe ◽  
Yumiko Ochiai ◽  
Miwa Aida ◽  
Keunchil Park ◽  
Seong-Jin Kim ◽  
...  

2009 ◽  
Vol 57 (12) ◽  
pp. 5211-5218 ◽  
Author(s):  
Yonghuan Zhao ◽  
Sam K. C. Chang ◽  
Gang Qu ◽  
Tianwen Li ◽  
Hongbin Cui

1994 ◽  
Vol 41 (10) ◽  
pp. 709-711 ◽  
Author(s):  
Yoko FUKE ◽  
Yoshie OHISHI ◽  
Keiko IWASHITA ◽  
Haruhiro ONO ◽  
Kazuki SHINOHARA

1993 ◽  
Vol 24 (6) ◽  
pp. 584-589 ◽  
Author(s):  
Hironobu Sasano ◽  
Fumiko Date ◽  
Akio Imatani ◽  
Shigeru Asaki ◽  
Hiroshi Nagura

2011 ◽  
Vol 22 (21) ◽  
pp. 215101 ◽  
Author(s):  
Sarah E Cross ◽  
Yu-Sheng Jin ◽  
Qing-Yi Lu ◽  
JianYu Rao ◽  
James K Gimzewski

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