Effect of alloxan on the incorporation of D-glucose into cultured pancreatic endocrine cells of the rat.

The aim of this experiment was to find out whether or not, at early stages of development, progenitors of the various types of gut endocrine cells are localized to one or more specific regions of the gastrointestinal tract. Transverse strips of blastoderm two to four somites in length were excised between the levels of somites 5 and 27 in chick embryos at 5- to 24-somite stages and were cultured as chorioallantoic grafts. The distribution of endocrine cells in the grafts revealed confined localization of progenitor cells only in the case of insulinimmunoreactive cells. Theprogenitors of cells with somatostatin-, pancreatic polypeptide-, glucagon-, secretin-, gastrin/CCK-, motilin-, neurotensin- and serotonin-like immunoreactivity were distributed along the length of the presumptive gut at the time of explantation; indeed, in many cases they were more widespread than are their differentiated progeny in normal gut of the same age. This finding indicates that conditions in grafts must differ from those that operate in the intact embryo. Also it may explain the occurrence of ectopic gut or pancreatic endocrine cells in tumours of the digestive tract.

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An immunohistochemical study of pancreatic endocrine cells in SKH-1 hairless mice

European Journal of Histochemistry ◽

10.4081/1684 ◽

2010 ◽

Vol 46 (3) ◽

pp. 229 ◽

Cited By ~ 4

Author(s):

SK Ku ◽

HS Lee ◽

JH Lee

Keyword(s):

Endocrine Cells ◽

Immunohistochemical Study ◽

Hairless Mice ◽

Pancreatic Endocrine Cells

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Maintenance of pancreatic endocrine cells of the neonatal rat: IV. The effect of 3-amino-3-deoxyglucose.

Endocrinologia Japonica ◽

10.1507/endocrj1954.31.657 ◽

1984 ◽

Vol 31 (5) ◽

pp. 657-664

Author(s):

SHIGERU WAKABAYASHI ◽

SHOHEI KAGAWA ◽

KEIKO NAKAO ◽

KEIKO YOSHIDA ◽

KOICHI MIMURA ◽

...

Keyword(s):

Endocrine Cells ◽

Neonatal Rat ◽

Pancreatic Endocrine Cells

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Effect of long-term culture on the expression of antigens and adhesion molecule in single porcine pancreatic endocrine cells

Xenotransplantation ◽

10.1111/j.1399-3089.2005.00232.x ◽

2005 ◽

Vol 12 (4) ◽

pp. 327-332 ◽

Cited By ~ 5

Author(s):

Kazuya Edamura ◽

Koko Nasu ◽

Yukiko Iwami ◽

Ryohei Nishimura ◽

Hiroyuki Ogawa ◽

...

Keyword(s):

Adhesion Molecule ◽

Endocrine Cells ◽

Long Term Culture ◽

Pancreatic Endocrine Cells

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Epigenetic-Mediated Reprogramming of Pancreatic Endocrine Cells

Antioxidants and Redox Signaling ◽

10.1089/ars.2014.6103 ◽

2015 ◽

Vol 22 (16) ◽

pp. 1483-1495 ◽

Cited By ~ 2

Author(s):

Prabhu Mathiyalagan ◽

Samuel T. Keating ◽

Keith Al-Hasani ◽

Assam El-Osta

Keyword(s):

Endocrine Cells ◽

Pancreatic Endocrine Cells

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REST is a major negative regulator of endocrine differentiation during pancreas organogenesis

10.1101/2021.03.17.435876 ◽

2021 ◽

Author(s):

Meritxell Rovira ◽

Goutham Atla ◽

Miguel Angel Maestro ◽

Vane Grau ◽

Javier García-Hurtado ◽

...

Keyword(s):

Endocrine Cells ◽

Negative Regulator ◽

Β Cell ◽

Knock Out ◽

Endocrine Differentiation ◽

Conditional Allele ◽

Embryonic Pancreas ◽

Pancreatic Endocrine Cells ◽

Knock Out Mice

SUMMARYUnderstanding genomic regulatory mechanisms of pancreas differentiation is relevant to the pathophysiology of diabetes mellitus, and to the development of replacement therapies. Numerous transcription factors promote β cell differentiation, although less is known about negative regulators. Earlier epigenomic studies suggested that the transcriptional repressor REST could be a suppressor of endocrine gene programs in the embryonic pancreas. However, pancreaticRestknock-out mice failed to show increased numbers of endocrine cells, suggesting that REST is not a major regulator of endocrine differentiation. Using a different conditional allele that enables profound REST inactivation, we now observe a marked increase in the formation of pancreatic endocrine cells. REST inhibition also promoted endocrinogenesis in zebrafish and mouse early postnatal ducts, and induced β-cell specific genes in human adult duct-derived organoids. Finally, we define REST genomic programs that suppress pancreatic endocrine differentiation. These results establish a crucial role of REST as a negative regulator of pancreatic endocrine differentiation.

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Co-expression of insulin and somatostatin genes in pancreatic endocrine cells selected for their high level of insulin gene transcription

Journal of Cell Science ◽

10.1242/jcs.101.4.795 ◽

1992 ◽

Vol 101 (4) ◽

pp. 795-799

Author(s):

C. Saulnier-Michel ◽

M. Fromont-Racine ◽

R. Pictet

Keyword(s):

Endocrine Cells ◽

Selective Pressure ◽

Cell Types ◽

Insulin Gene ◽

Regulatory Sequences ◽

Endogenous Insulin ◽

Pancreatic Endocrine Cells ◽

Gene Regulatory ◽

High Level ◽

Two Populations

RW cells are pancreatic endocrine RIN cells that have been stably transfected with a chimeric gene that places the expression of the dominant selection gpt gene under the control of the insulin gene regulatory sequences. These RW cells were examined for hormone content using immunocytochemistry. This analysis shows that: first, there are cells that are negative for insulin although they were cultured under selective pressure. Second, there is a higher proportion of somatostatin-producing cells than in the parental RIN cells; these somatostatin cells form two populations: one of cells containing only somatostatin and, surprisingly, one made of cells containing both insulin and somatostatin. Thus: (1) expression of the transfected and endogenous insulin regulatory sequences is not regulated in a coordinate fashion; (2) the presence of both hormones in the same cell suggests that the regulation of the expression of insulin and somatostatin genes and the differentiation pathway of the two respective cell types may be closely related.

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