scholarly journals Role of a Novel Protein, CAR-like Soluble Protein (CLSP), in Adenovirus Infection

2007 ◽  
Vol 127 (7) ◽  
pp. 1091-1096 ◽  
Author(s):  
Kenji KAWABATA
Keyword(s):  
Soluble Protein ◽  
Adenovirus Infection ◽  
Novel Protein

TRiC-P5, a novel TCP1-related protein, is localized in the cytoplasm and in the nuclear matrix

1994 ◽  
Vol 107 (10) ◽  
pp. 2851-2859
Author(s):  
E.C. Joly ◽  
E. Tremblay ◽  
R.M. Tanguay ◽  
Y. Wu ◽  
V. Bibor-Hardy
Keyword(s):  
Nuclear Matrix ◽  
Cellular Localization ◽  
Cell Fractionation ◽  
Cellular Functions ◽  
Raji Cells ◽  
T Complex ◽  
Ring Complex ◽  
Filament Network ◽  
Novel Protein

We have recently reported the cloning of a novel protein, TRiC-P5, with significant homology with protein 1 of the t-complex (TCP1). In the present study, the cellular localization of TRiC-P5 in Raji cells has been determined using an antiserum raised against a 18.5 kDa fusion protein. Results from cell fractionation and immunoblot studies indicate that TRiC-P5 is mainly localized in the cytoplasm. In addition, a significant part of TRiC-P5 is also found in the nucleus where it is attached to the nuclear matrix, a complex filament network involved in essential cellular functions such as DNA replication, and RNA transcription and maturation. Immunofluorescence experiments using the anti-TRiC-P5 antibodies confirm these results. We also provide evidence that, in the cytoplasm, TRiC-P5 is part of a large protein complex, most probably the TCP1-ring complex (TRiC), a hetero-oligomeric ring complex that plays a role of molecular chaperone in the folding of actin and tubulin.

Evolution of primate orphan proteins

2009 ◽  
Vol 37 (4) ◽  
pp. 778-782 ◽  
Author(s):  
Macarena Toll-Riera ◽  
Robert Castelo ◽  
Nicolás Bellora ◽  
M. Mar Albà
Keyword(s):  
Related Species ◽  
Sequence Divergence ◽  
Orphan Genes ◽  
Closely Related Species ◽  
Protein Coding ◽  
Rapid Sequence ◽  
Adaptive Processes ◽  
Mechanisms Of Formation ◽  
Novel Protein

Genomes contain a large number of genes that do not have recognizable homologues in other species. These genes, found in only one or a few closely related species, are known as orphan genes. Their limited distribution implies that many of them are probably involved in lineage-specific adaptive processes. One important question that has remained elusive to date is how orphan genes originate. It has been proposed that they might have arisen by gene duplication followed by a period of very rapid sequence divergence, which would have erased any traces of similarity to other evolutionarily related genes. However, this explanation does not seem plausible for genes lacking homologues in very closely related species. In the present article, we review recent efforts to identify the mechanisms of formation of primate orphan genes. These studies reveal an unexpected important role of transposable elements in the formation of novel protein-coding genes in the genomes of primates.

scholarly journals Dual role of the adenovirus pVI C terminus as a nuclear localization signal and activator of the viral protease

2004 ◽  
Vol 85 (11) ◽  
pp. 3367-3376 ◽  
Author(s):  
K. S. Honkavuori ◽  
B. D. Pollard ◽  
M. S. Rodriguez ◽  
R. T. Hay ◽  
G. D. Kemp
Keyword(s):  
Nuclear Localization ◽  
Nuclear Localization Signal ◽  
Human Adenovirus ◽  
Dual Role ◽  
Adenovirus Infection ◽  
Heterologous Proteins ◽  
Viral Protease ◽  
C Terminus ◽  
Localization Signal

Adenain, the protease produced by adenovirus, is regulated by formation of a heterodimer with an 11 aa peptide derived from the C terminus of another adenoviral protein, pVI. Here, the role of the basic motif KRRR, which is conserved in pVI sequences from human adenovirus serotypes, was investigated. It was shown that this motif is less important than the N- or C-terminal regions in the formation of the adenain–peptide heterodimer and in the activity of the subsequent complex. This motif, however, acted as a nuclear localization signal that was capable of targeting heterologous proteins to the nucleus, resulting in a distinctive intranuclear distribution consisting of discrete foci, which is similar to that found for pVI during adenovirus infection.

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