Impact of Malignancies in the Early and Late Time Course of Takotsubo Cardiomyopathy

Exposure of humans to ambient levels of ozone causes inflammatory changes within lung tissues. These changes have been reported for the "initial" (1- to 3-h) and "late" (18- to 20-h) postexposure periods. We hypothesized that at the late period when protein and cellular markers of inflammation in bronchoalveolar lavage remain abnormal, permeability of respiratory epithelia would be altered. To test this, we measured by gamma-camera imagery the clearance kinetics in healthy subjects (n = 9) of 99mTc-labeled solute [diethylenetriaminepentaacetic acid (DTPA)] that was deposited by aerosol onto epithelial surfaces 19 +/- 1 h after a single exposure to ozone (O3; 130 min at ambient levels between 150 and 350 parts per billion and alternate periods of rest and moderate exercise) or filtered air. At the late period, the lung clearance of 99mTc-DTPA over a 120-min period was significantly increased, i.e., 0.732%/min for O3 exposures compared with 0.661%/min for filtered-air exposures (P < 0.05). Regional analysis demonstrated that 99mTc-DTPA clearance from the periphery (excluding the lung hilum) and lung apexes were significantly increased by O3 but changes in clearance for the base of the lung were not significant. The forced expiratory volume in 1 s at the late time after O3 was slightly but significantly reduced (-2.1%) from preexposure levels. There was no relationship between the functional changes observed acutely after exposure to O3 and subsequent changes in 99mTc-DTPA clearance or forced expiratory volume in 1 s observed at the late period. These results suggest that epithelial permeability of the lung is altered 18-20 h post-O3; this injury is regional, and the lung base appears to have a different time course of response or is in an adapted state with respect to O3 exposure.

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Time Course of Functional Recovery and ECG Change in Takotsubo Cardiomyopathy

Journal of Cardiovascular Imaging ◽

10.4250/jcvi.2019.0119 ◽

2020 ◽

Vol 28 (1) ◽

pp. 61

Author(s):

Yong Hyun Park

Keyword(s):

Functional Recovery ◽

Takotsubo Cardiomyopathy ◽

Time Course

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Takotsubo cardiomyopathy: which role for serial electrocardiogram changes?

International Cardiovascular Forum Journal ◽

10.17987/icfj.v5i0.200 ◽

2016 ◽

Vol 5 ◽

Cited By ~ 1

Author(s):

Loris Roncon ◽

Marco Zuin

Keyword(s):

Left Ventricle ◽

Takotsubo Cardiomyopathy ◽

Clinical Management ◽

Time Course ◽

Acute Stress ◽

Systolic Dysfunction ◽

Ecg Changes ◽

First Line ◽

Cardiac Syndrome

<p>Takotsubo cardiomyopathy (TTC) is a cardiac syndrome characterized by acute regional-reversible systolic dysfunction of the left ventricle (LV) and related to psycho-physical acute stress. Recently, physicians and researchers have posed their attention on the time course of ECG changes in TTC. Knowledge of ECG changes in TTC could be useful for the clinical management of this disease. Among all TTC deaths, 38% of cases are directly related to in-hospital complications. Serial ECG assessment could be a first line defence in reducing mortality and in-hospital complications in patients with TTC. </p>

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In Vitro Study of the Effects of Precore and Lamivudine-Resistant Mutations on Hepatitis B Virus Replication

Journal of Virology ◽

10.1128/jvi.02341-06 ◽

2007 ◽

Vol 81 (7) ◽

pp. 3068-3076 ◽

Cited By ~ 9

Author(s):

Richard A. Heipertz ◽

Thomas G. Miller ◽

Colleen M. Kelley ◽

William E. Delaney ◽

Stephen A. Locarnini ◽

...

Keyword(s):

Hepatitis B Virus ◽

Hepatitis B ◽

Time Course ◽

Recombinant Baculovirus ◽

Hbv Dna ◽

Late Time ◽

Time Points ◽

Hbv Replication ◽

B Virus

ABSTRACT Understanding the consequences of mutation in the hepatitis B virus (HBV) genome on HBV replication is critical for treating chronic HBV infection. In this study, HBV replication in HepG2 cells initiated by transduction with precore (PC), rtM204I, and wild-type (wt) HBV recombinant baculoviruses was compared. The pattern and magnitude of HBV replication initiated by the PC HBV recombinant baculovirus were similar to those observed for wt HBV throughout the time course examined. In contrast, when the rtM204I mutation was introduced into wt HBV, by day 10 postinfection the levels of intra- and extracellular HBV DNA were markedly reduced compared to those for wt HBV. Although the rtM204I mutation reduced the production of HBV replicative intermediates, no effect on the level of covalently closed circular DNA or HBV transcripts was observed at late time points. Coinfection studies with different ratios of wt and rtM204I baculoviruses showed that the rtM204I variant did not produce a product that inhibited HBV replication. However, the combination of the wt and rtM204I baculoviruses yielded HBV DNA levels at late time points that were greater than those for the wt alone, suggesting that wt polymerase may function in trans to boost rtM204I replication. We concluded that the rtM204I mutation generates a polymerase that is not only resistant to lamivudine but also replicates nucleic acids to lower levels in vitro.

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Evaluation of the arrhythmogenecity of Stress-Induced “Takotsubo cardiomyopathy” from the time course of the 12-lead surface electrocardiogram

The American Journal of Cardiology ◽

10.1016/s0002-9149(03)00547-2 ◽

2003 ◽

Vol 92 (2) ◽

pp. 230-233 ◽

Cited By ~ 91

Author(s):

Koji Matsuoka ◽

Setsuya Okubo ◽

Eitaro Fujii ◽

Fumiya Uchida ◽

Atsunobu Kasai ◽

...

Keyword(s):

Takotsubo Cardiomyopathy ◽

Time Course ◽

Surface Electrocardiogram

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Genome-wide kinetics of DNA excision repair in relation to chromatin state and mutagenesis

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.1603388113 ◽

2016 ◽

Vol 113 (15) ◽

pp. E2124-E2133 ◽

Cited By ~ 76

Author(s):

Sheera Adar ◽

Jinchuan Hu ◽

Jason D. Lieb ◽

Aziz Sancar

Keyword(s):

Time Course ◽

Excision Repair ◽

Early Time ◽

Chromatin State ◽

Open Chromatin ◽

Late Time ◽

Normal Human Skin ◽

Time Points ◽

Dna Excision Repair ◽

Genome Wide

We recently developed a high-resolution genome-wide assay for mapping DNA excision repair named eXcision Repair-sequencing (XR-seq) and have now used XR-seq to determine which regions of the genome are subject to repair very soon after UV exposure and which regions are repaired later. Over a time course, we measured repair of the UV-induced damage of cyclobutane pyrimidine dimers (CPDs) (at 1, 4, 8, 16, 24, and 48 h) and (6-4)pyrimidine-pyrimidone photoproducts [(6-4)PPs] (at 5 and 20 min and 1, 2, and 4 h) in normal human skin fibroblasts. Each type of damage has distinct repair kinetics. The (6-4)PPs are detected as early as 5 min after UV treatment, with the bulk of repair completed by 4 h. Repair of CPDs, which we previously showed is intimately coupled to transcription, is slower and in certain regions persists even 2 d after UV irradiation. We compared our results to the Encyclopedia of DNA Elements data regarding histone modifications, chromatin state, and transcription. For both damage types, and for both transcription-coupled and general excision repair, the earliest repair occurred preferentially in active and open chromatin states. Conversely, repair in regions classified as “heterochromatic” and “repressed” was relatively low at early time points, with repair persisting into the late time points. Damage that remains during DNA replication increases the risk for mutagenesis. Indeed, late-repaired regions are associated with a higher level of cancer-linked mutations. In summary, we show that XR-seq is a powerful approach for studying relationships among chromatin state, DNA repair, genome stability, mutagenesis, and carcinogenesis.

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Globin synthesis in heterokaryons formed between chick erythrocytes and human K562 cells or rat L6 myoblasts

Journal of Cell Science ◽

10.1242/jcs.66.1.309 ◽

1984 ◽

Vol 66 (1) ◽

pp. 309-319

Author(s):

G. Lanfranchi ◽

S. Linder ◽

N.R. Ringertz

Keyword(s):

Time Course ◽

Early Time ◽

K562 Cells ◽

Late Time ◽

Globin Chain ◽

Time Points ◽

Erythroleukemia Cells ◽

Globin Synthesis ◽

Chain Synthesis ◽

Globin Chain Synthesis

Chick globin synthesis was studied in heterokaryons formed between chick erythrocytes and human K562 erythroleukemia cells or rat L6J1 myoblasts. It was found that chick globin synthesis was activated after fusion of definitive (17 to 19-day) chick erythrocytes obtained from 17 to 19-day embryos with K562 cells. Chick globins appeared to be of the adult alpha A, alpha D and beta types, whereas no embryonic globin synthesis could be detected. The pattern and time-course of globin synthesis was investigated after fusion of 4 to 5-day embryonic erythrocytes with rat L6J1 myoblasts. The level of globin synthesis was high at early time points but then decreased. Globin synthesis, however, was still detectable at 9 days in these heterokaryons. Chick alpha A, alpha D and epsilon-globin chain synthesis was observed both at early and late time points after fusion.

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The effect of talker familiarity on sentence recognition accuracy over time

10.31219/osf.io/jtpc7 ◽

2020 ◽

Author(s):

Madison Buntrock ◽

Brittan Ann Barker ◽

Madison M. Guires ◽

Tyson S. Barrett

Keyword(s):

Time Course ◽

Recognition Accuracy ◽

Early Time ◽

Late Time ◽

Sentence Recognition ◽

Mixed Effects Modeling ◽

University Professor ◽

Talker Familiarity ◽

Over Time

The familiar talker advantage (FTA) reflects the robust research finding that a listener’s ability to perceive and understand a talker in noise is facilitated when the listener is familiar with the talker. However, it is unclear when the benefits of said familiarity first emerge and whether or not they strengthen over time. To better understand the time course of the FTA, we assessed the effects of long-term, implicit voice learning on 89 young adults’ sentence recognition accuracy in the presence of 4-talker babble. A university professor served as the target talker in the experiment. Half the participants were students of the professor and familiar with her voice. The professor was a stranger to the remaining participants. We manipulated the listeners’ degree of familiarity with the professor over the course of a semester. To test for the effects of the two independent variables: talker (familiar, novel) and time of testing (early: Time 1, late: Time 2), we used mixed effects modeling. Analyses revealed that an FTA emerged in the listeners after only 16 weeks (~32 hours) of exposure to the target voice in a college classroom setting. The FTA also strengthened over time. Implicit exposure to the target talker’s voice for ~12 hours over approximately 4 weeks was not enough time to yield an FTA. The present results imply that talker familiarity (outside of the confines of a long-term, familial relationship) seems to be a much quicker-to-emerge and reliable cue for bootstrapping spoken language perception than previous literature suggested.

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Transcriptional response to kairomone exposure in Daphnia magna: A candidate gene approach

10.21203/rs.2.20016/v1 ◽

2020 ◽

Author(s):

Murat Telli ◽

Donna M. Gordon ◽

Ercan Selçuk Ünlü

Keyword(s):

Daphnia Magna ◽

Time Course ◽

Biological Activities ◽

Predatory Fish ◽

Candidate Gene Approach ◽

Late Time ◽

Mrna Transcript ◽

Transcript Levels ◽

Fish Kairomone ◽

Ideal System

Abstract Background: Daphnia (Brachiopoda, Cladocera) is a well-studied model organism providing unparalleled opportunity to test epigenetic regulation of predator avoidance mechanisms in aquatic ecosystems. The discovery of regulatory functions for microRNA molecules and recently described miRNA profiles of Daphnia make it an ideal system to probe for posttranslational regulatory mechanisms mediated by kairomone released by predatory fish. However, despite a number of studies that focused on mRNA transcript level differences, no miRNA studies associated with kairomone exposure have been reported. Results: Exposing D. magna to fish kairomone from birth to the first reproduction was found to result in the differential expression of the four miRNAs tested: miR-7, miR-34, miR-317, and miR-375. Normalized transcript levels for each miRNA were found to vary across the exposure period with no clear conserved pattern of expression despite functional target analyses by GO, COG and KEGG indicating that predicted miRNA target genes are likely involved in related biological activities. Analysis of six mRNA transcripts (Hsp70, Hsp90, actin, AKT, GYS and IGFR), identified in previous studies as kairomone-mediated genes in Daphnia magna, were also carried out. Similar to that obtained for miRNAs, the mRNA transcript levels showed varying degrees of temporal regulation across the exposure time course with the two heat shock transcripts exhibiting elevated levels at early and late time points of kairomone exposure while the AKT, GYS, and IGFR transcripts had an general decrease in expression during the first 96 hours. Conclusions: Differential mRNA expression data supports the premise of an ecological trade-off between the cost of general biological processes and that of survival under long-term kairomone stress. Transcript levels for the four miRNAs tested were found to vary across developmental time with kairomone exposure which suggests that they may have a role in regulating morphological, behavioral or physiological responses by altering target gene expression. These studies lay the foundation for future work aimed at linking miRNAs and their target transcripts to changes in the signaling events that govern Daphnia response to kairomone specific stress.

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Ultrastructural Changes of the Symbiotic Chlorella-Like Alga Isolated from Hydra Viridis

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100054212 ◽

1982 ◽

Vol 40 ◽

pp. 320-321

Author(s):

K.W. Lee ◽

R.H. Meints ◽

D. Kuczmarski ◽

J.L. Van Etten

Keyword(s):

Time Course ◽

Reciprocal Cross ◽

Ultrastructural Level ◽

Ultrastructural Changes ◽

Symbiotic Relationship ◽

Cell Recognition ◽

Green Hydra ◽

Different Strains ◽

Hydra Viridis

The physiological, biochemical, and ultrastructural aspects of the symbiotic relationship between the Chlorella-like algae and the hydra have been intensively investigated. Reciprocal cross-transfer of the Chlorellalike algae between different strains of green hydra provide a system for the study of cell recognition. However, our attempts to culture the algae free of the host hydra of the Florida strain, Hydra viridis, have been consistently unsuccessful. We were, therefore, prompted to examine the isolated algae at the ultrastructural level on a time course.

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