scholarly journals Recirculation anaesthesia for laboratory fish

1987 ◽  
Vol 21 (3) ◽  
pp. 210-215 ◽  
Author(s):  
L. A. Brown

An economic reliable long-term recirculation anaesthesia system for laboratory fish is described. Anaesthesia of channel catfish ( Ictalurus punctatus Rafinesque) was induced within 60 s and was maintained for up to 40 min using tricaine methanesulphonate; recovery occurred within 30-60 s. Various surgical procedures were performed on the fish. No deaths were recorded. All water-quality parameters tested over 19 days use of the system remained stable except for total ammonia nitrogen and, by calculation, un-ionized ammonia which increased to a maximum of 0·23 mg/1.

2017 ◽  
Vol 44 (3) ◽  
pp. 638-642
Author(s):  
Janaína S. Pedron ◽  
Denise S. Miron ◽  
Ricardo V. Rodrigues ◽  
Marcelo H. Okamoto ◽  
Marcelo B. Tesser ◽  
...  

This experiment evaluated the efficacy of benzocaine to reduce stress response during transport of juvenile cobia. Fish (30 g) were packed in bags and transported for 8 h (stocking density = 10 g L-1). Three concentrations of benzocaine were evaluated: 0, 2, and 6 mg L-1. Blood samples were taken for glucose and hematocrit before transportation, and then at 0, 2, 24, and 48 h after. Water quality parameters were verified. No mortality was observed. Total ammonia nitrogen was higher (2.46 mg L-1) and pH was lower (6.92) at 2 mg benzocaine L-1. There was an increase in blood glucose for all treatments on arrival, and it was higher for those exposed to benzocaine at 6 mg L-1, although at 48 h they were all similar. The hematocrit did not differ among treatments. The results suggest: 1) the density 10 g L-1 is considered safe for juvenile cobia transport; 2) benzocaine did not mitigate stress response on cobia during transport, therefore its use is not recommended for this purpose.


2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Bharat Nandkumar Mulay ◽  
Konda Rajasekhar Reddy

Abstract This experimental investigation of a laboratory scale aquaponic system included testing of a biofilter with basil plant as the biofilter part on aquaculture water quality. Irridescent shark was selected as aquaculture species. The biofilter consist of natural biomaterials such as coconut husk, coco peat, and coarse aggregates. The experiment was carried out for three short water recirculation durations of 2, 3, and 4 h/day. The influent and effluent ammonia NH3, total ammonia nitrogen (TAN), nitrites NO2 and nitrates NO3 levels were measured and analysed. The results showed that the biofilter effectively removed NH3 (65 - 71 %), TAN (34 - 58 %), and NO2 (60 - 67 %) from the aquaculture water. The dissolved oxygen (DO) levels were maintained between 3 - 7.0 mg/l during all the recirculation durations. The significance of water recirculation period was assessed by calculating the differences between the means of water quality parameters with a statistical test named one-way analysis of variance (ANNOVA) with significant level P taken as 5 %, i.e., P ≤ 0.5. The effluent mean NH3 levels 0.030 mg/l, 0.033 mg/l, and 0.022 mg/l exhibited significant difference at 4 h/d periods while effluent TAN levels 0.81, 0.77, and 0.77 showed no difference with varying periods.


Author(s):  
Biji Xavier ◽  
Sekar Megarajan ◽  
Ritesh Ranjan ◽  
Ponnaganti Shiva ◽  
Biswajit Dash ◽  
...  

<p>Effect of different packing densities on water quality parameters, survival and selected tissue biochemical parameters during transportation of hatchery produced fingerlings of orange spotted grouper <em>Epinephelus coioides</em> (Hamilton, 1822) was investigated. Fingerlings (weight 3.0±0.2 g and length 6.0±0.2 cm) were packed in sealed double layered oxygen packed polythene bags (water and oxygen ratio 1:3) at different packing densities of 20, 30, 40 and 50 no. l-1. The packed fishes were transported for 6 h. After transportation, water samples and tissue samples from fishes were collected for further analyses. Levels of tissue glucose and selected metabolic enzymes (lactate dehydrogenase, LDH; aspartate amino transferase, AST and alanine amino transferase, ALT) significantly (p&lt;0.05) increased with increased packing density. Water quality parameters <em>viz.,</em> pH, dissolved oxygen, CO2, alkalinity, total ammonia nitrogen (TAN) and nitrite nitrogen (NO2-N) were also significantly different at higher packing densities (p&lt;0.05). However, levels of all the tissue biochemical parameters tested were in tolerable range and no mortality of fingerlings was recorded at any of the packing densities. Though the tissue enzyme levels were significantly higher and water quality was significantly deteriorated at the highest packing density of 50 no. l-1, it did not lead to mortality of fish. Therefore, it is inferred that this density can be used for short distance transportation of fingerlings of orange spotted grouper.</p>


Author(s):  
Mengmeng Yi ◽  
Chun Wang ◽  
He Wang ◽  
Xi Zhu ◽  
Zhigang Liu ◽  
...  

Abstract In the present study, we investigated the effect of probiotics immobilized by oyster shells (Os), vesuvianite (Ve) and walnut shells (Ws) on the remediation of aquaculture water and sediment by analyzing the variation of ammonia-nitrogen (NH4–N), nitrate-nitrogen (NO3–N), nitrite-nitrogen (NO2–N), total nitrogen (TN), total phosphorus (TP) and chemical oxygen demand (CODCr), as well as the microbiota of the water and sediment. The positive or negative effects of the treatment groups on the water quality parameters were both observed. Compared with their effects on water quality parameters, the treatment groups had better effects on sediment parameters. Group Ve had the best remediation effect of NH4–N and NO3–N in the sediment (decreased by 5.22 and 1.66 times, respectively). Group Os showed a lower relative concentration of TN and CODCr (decreased by 3.77 and 0.95 times, respectively). The high-throughput sequencing results revealed that the immobilized probiotics increased the relative abundances of functional bacteria in the treatment groups at the phylum and genus level. The above results showed that probiotics immobilized by oyster shells, vesuvianite and walnut shells positively affected the aquaculture environment's remediation, especially the sediment.


2017 ◽  
Vol 39 (5) ◽  
pp. 601
Author(s):  
Davi De Holanda Cavalcante ◽  
Francisco Roberto Dos Santos Lima ◽  
Vanessa Tomaz Rebouças ◽  
Marcelo Vinícius do Carmo e Sá

The present study aimed to assess the possible beneficial effects of the integration between bioflocs and periphyton to the Nile tilapia’s water quality and growth performance. There were four treatments with five replicates each: (1) Control: green waters, (2) Periphyton: substrate-based system, (3) BFT: bioflocs technology for aquaculture, and (4) Biophyton: integration between bioflocs and periphyton. Fish (1.63 ± 0.07 g) were reared for 10 weeks in twenty 250 L outdoor tanks. Two polyethylene boards were vertically set out in the Periphyton and Biophyton tanks as underwater substrates. The C: N ratios of water in the BFT and Biophyton tanks were adjusted to 15:1 with dry molasses applications. The concentrations of total ammonia nitrogen were higher in the Control and Periphyton tanks than in the BFT and Biophyton ones. On the other hand, the concentrations of reactive phosphorus were higher in the BFT and Biophyton tanks than in the Control and Periphyton ones. The fish final body weight, specific growth rate and fish yield have not differed between the tanks. The integration between bioflocs and periphyton has not brought clear benefits to tilapia culture on water quality and growth performance.  


2018 ◽  
Vol 44 (4) ◽  
pp. 660-669 ◽  
Author(s):  
Katya E. Kovalenko ◽  
Euan D. Reavie ◽  
Richard P. Barbiero ◽  
Lyubov E. Burlakova ◽  
Alexander Y. Karatayev ◽  
...  

Author(s):  
Emily E Grausgruber ◽  
Michael J. Weber

Fish hatcheries are generally not adjacent to stocking locations. Thus, hatchery fish undergo transportation processes for variable durations that can result in changes in water quality, fish physiology (e.g., whole blood glucose and plasma cortisol), and reduced survival. Walleye Sander virteus are commonly stocked throughout North America with variable stocking success, possibly due to altered physiological responses associated with changes in water quality parameters during transportation. We hypothesized increased transport duration would be associated with increases in water temperature, carbon dioxide, and total ammonia nitrogen and decreases in pH and total alkalinity. We also hypothesized that increases in carbon dioxide, water temperature, unionized ammonia, and total ammonia nitrogen would be positively related with Walleye whole blood glucose and plasma cortisol concentrations. Walleye were transported either 0, 0.5, 3, or 5 hours and whole blood glucose and plasma cortisol concentrations and mortality were evaluated for 48 hours post-transport. Total ammonia nitrogen concentrations, carbon dioxide, pH, and water temperature increased with transportation duration while total alkalinity decreased. Plasma cortisol and whole blood glucose concentrations of Walleye transported longer durations took longer to decline relative to those not transported. Water quality parameters were not associated with changes in Walleye whole blood glucose and plasma cortisol concentrations, but they were negatively related with time since transport (P &lt; 0.05). Despite increases in stress, mortality was low (2.5%). Overall, we found evidence to support our hypotheses regarding reduced water quality associated with increasing transport duration. Finally, whole blood glucose and plasma cortisol concentration of Walleye transported were similar to those not transported, suggesting handling procedures before transportation could play a significant role in physiological responses measured after transportation. Further, evaluation of stocking procedures aimed at decreasing handling during the loading process could enhance stocking protocols..Elevated concentrations of whole blood glucose and plasma cortisol following transport could make recently stocked Walleye more susceptible to the effects of other environmental factors such as starvation, predation, and disease, all of which could result in increased mortality rates after stocking.


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