Specific detection of mousepox virus by polymerase chain reaction
Keyword(s):
Polymerase chain reaction was applied to the rapid identification and detection of mousepox virus. This was accomplished by selection of primers targeting the A-type inclusion body protein gene. By investigating 20 strains belonging to five different species of the genus Orthopoxvirus, amplification was achieved only with the seven mousepox virus strains examined. The size of the resulting DNA fragment accounted for 116 base pairs and contained a recognition site for the restriction enzyme HindII, thus confirming its viral origin. Amplification of mousepox virus specific sequences was also possible from infected mouse lung tissue and serum.
1997 ◽
Vol 64
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pp. 217-221
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1991 ◽
Vol 66
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pp. 500-504
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1995 ◽
Vol 99
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pp. 1483-1488
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1994 ◽
Vol 8
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pp. 51-54
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2016 ◽
Vol 18
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pp. 91
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2019 ◽
Vol 273
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pp. 113713
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2017 ◽
Vol 58
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pp. 113-123
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2010 ◽
Vol 90
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pp. 357-360
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