Protective Effect of Puerarin Against Oxidative Stress Injury of Neural Cells and Related Mechanisms

Atherosclerosis is closely associated with the inflammatory reaction of vascular endothelial cells. Puerarin (Pue), the main active component isolated from the rhizome of Pueraria lobata, is an isoflavone compound with potent antioxidant properties. Although Pue exhibits promising antiatherosclerotic pharmacological effects, only a few studies have reported its protective effect on endothelial cells. This study found that Pue could partly regulate mitochondrial function in human umbilical vein endothelial cells (HUVECs) and reduce or inhibit lipopolysaccharide-induced inflammatory reactions and oxidative stress injury in HUVECs, likely via mitochondrial quality control. Furthermore, the protective effect of Pue on HUVECs was closely related to the SIRT-1 signaling pathway. Pue increased autophagy and mitochondrial antioxidant potential via increased SIRT-1 expression, reducing excessive production of ROS and inhibiting the expression of inflammatory factors and oxidative stress injury. Therefore, Pue may improve mitochondrial respiratory function and energy metabolism, increasing the vulnerability of HUVECs to an inflammatory state.

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Protective effect of Xiongma pills-containing serum on SNP oxidative stress-injury in PC12 cells

The 2011 IEEE/ICME International Conference on Complex Medical Engineering ◽

10.1109/iccme.2011.5876736 ◽

2011 ◽

Cited By ~ 1

Author(s):

Lin Liu ◽

Ying Zhao ◽

Jiling Jiang ◽

Ying Tan

Keyword(s):

Oxidative Stress ◽

Protective Effect ◽

Pc12 Cells ◽

Stress Injury ◽

Oxidative Stress Injury

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Protective Effect on Oxidative Stress Injury of Xiongma Dripping Pills Containing Serum on PC12 Cells

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.955-959.744 ◽

2014 ◽

Vol 955-959 ◽

pp. 744-747

Author(s):

Lin Liu ◽

Jia Yi Xia ◽

Ju Huo ◽

Zhao Ying

Keyword(s):

Oxidative Stress ◽

Plasma Concentration ◽

Oxidative Damage ◽

Protective Effect ◽

Pc12 Cells ◽

Dose Group ◽

Dose Effect ◽

Cell Culture Supernatant ◽

Stress Injury ◽

Oxidative Stress Injury

Object: The antioxidant dose-effect relationship and the mechanism of Xiongma Dripping Pills were study to explore the protective effects of oxidative damage in PC12 cells of different doses of Xiongma Dripping Pills containing serum. Method: Oxidative stress injury model of PC12 cells was established by peroxide hydrogen (H2O2), sodium nitroprusside (SNP), content and cell culture supernatant lactate dehydrogenase (LDH) and the liquid content of glutathione peroxidase (GSH-PX) were detected by chemical colorimetric determination. Results: There was a plasma concentration peaked in Xiongma Dripping Pills 8 times (6g•kg-1) dose group, ferulic acid plasma concentration was 10.59 ± 2.92, the plasma concentration gastrodin 18.13 ± 4.63. H2O2 and SNP could cause injury in PC12 cells, and increased LDH leakage, reduce the content of GSH-PX (P<0.01). Gung Ma the Dripping Pills can reduce LDH leakage, increase the content of GSH-PX. Xiongma Dripping Pills 8-fold dose group content is the most significant.Conclusion: There was a significant protective effect on Xiongma Dripping Pills serum containing H2O2 and SNP induced oxidative damage of PC12 cells and its mechanism was relevant with clearing the free radicals, enhanceing antioxidant enzyme activity in vivo. There was the most significant effect on Xiongma Dripping Pills 8-fold dose group, and there was positive correlation on plasma concentration and pharmacological effects.

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