scholarly journals Cloning, nucleotide sequence, and expression of tef-1, the gene encoding translation elongation factor 1.ALPHA. (EF-1.ALPHA.) of Neurospora crassa.

1995 ◽  
Vol 70 (2) ◽  
pp. 273-287 ◽  
Author(s):  
Akihiko ICHI-ISHI ◽  
Hirokazu INOUE
Keyword(s):  
Nucleotide Sequence ◽  
Neurospora Crassa ◽  
Elongation Factor ◽  
Translation Elongation Factor ◽  
Gene Encoding ◽  
Translation Elongation ◽  
Elongation Factor 1 Alpha ◽  
Elongation Factor 1

scholarly journals Increased expression of Saccharomyces cerevisiae translation elongation factor 1 alpha bypasses the lethality of a TEF5 null allele encoding elongation factor 1 beta.

Genetics ◽  
1995 ◽  
Vol 141 (2) ◽  
pp. 481-489 ◽  
Author(s):  
T G Kinzy ◽  
J L Woolford
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Null Allele ◽  
Elongation Factor ◽  
Wild Type ◽  
Translation Elongation Factor ◽  
Gene Encoding ◽  
Translation Elongation ◽  
Dominant Mutant ◽  
Cold Sensitive ◽  
Elongation Factor 1

Abstract Translation elongation factor 1beta (EF-1beta) catalyzes the exchange of bound GDP for GTP on EF-1alpha. The lethality of a null allele of the TEF5 gene encoding EF-1beta in Saccharomyces cerevisiae was suppressed by extra copies of the TEF2 gene encoding EF-1alpha. The strains with tef5::TRP1 suppressed by extra copies of TEF were slow growing, cold sensitive, hypersensitive to inhibitors of translation elongation and showed increased phenotypic suppression of +1 frameshift and UAG nonsense mutations. Nine dominant mutant alleles of TEF2 that cause increased suppression of frameshift mutations also suppressed the lethality of tef5::TRP1. Most of the strains in which tef5::TRP1 is suppressed by dominant mutant alleles of TEF2 grew more slowly and were more antibiotic sensitive than strains with tef5::TRP1 is suppressed by wild-type TEF2. Two alleles, TEF2-4 and TEF2-10, interact with tef5::TRP1 to produce strains that showed doubling times similar to tef5::TRP1 strains containing extra copies of wild-type TEF2. These strains were less cold sensitive, drug sensitive and correspondingly less efficient suppressor of +1 frameshift mutations. These phenotypes indicate that translation and cell growth are highly sensitive to changes in EF-1alpha and EF-1beta activity.

scholarly journals Four new East Asian species of Aleurodiscus with echinulate basidiospores

MycoKeys ◽  
2019 ◽  
Vol 52 ◽  
pp. 71-87
Author(s):  
Sheng-Hua Wu ◽  
Chia-Ling Wei ◽  
Yu-Ting Lin ◽  
Chiung-Chih Chang ◽  
Shuang-Hui He
Keyword(s):  
New Species ◽  
Southwest China ◽  
East Asian ◽  
Elongation Factor ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Northwest Yunnan ◽  
Elongation Factor 1 Alpha ◽  
Alpha Gene ◽  
Elongation Factor 1

Four new species of Aleurodiscus sensu lato with echinulate basidiospores are described from East Asia: A.alpinus, A.pinicola, A.senticosus, and A.sichuanensis. Aleurodiscusalpinus is from northwest Yunnan of China where it occurs on Rhododendron in montane habitats. Aleurodiscuspinicola occurs on Pinus in montane settings in Taiwan and northwest Yunnan. Aleurodiscussenticosus is from subtropical Taiwan, where it occurs on angiosperms. Aleurodiscussichuanensis is reported from southwest China on angiosperms in montane environments. Phylogenetic relationships of these four new species were inferred from analyses of a combined dataset consisting of three genetic markers, viz. 28S, nuc rDNA ITS1-5.8S-ITS2 (ITS), and a portion of the translation elongation factor 1-alpha gene, TEF1.

scholarly journals First report of Fusarium solani causing stem rot of Dracaena in Iran

2016 ◽  
Vol 56 (1) ◽  
pp. 100-103 ◽  
Author(s):  
Mostafa Abedi-Tizaki ◽  
Doustmorad Zafari ◽  
Jamal Sadeghi
Keyword(s):  
Fusarium Solani ◽  
Elongation Factor ◽  
Stem Rot ◽  
Pathogenicity Test ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
First Report ◽  
Brown Discoloration ◽  
Elongation Factor 1 Alpha ◽  
Elongation Factor 1

Abstract In July 2013, symptoms of stem rot were observed in the Dracaena sanderiana cuttings in greenhouses of Mahallat County, Markazi Province, Iran. The symptoms first appeared as severe wilting. Later, leaves became brown and necrotic. Symptoms on the cuttings were observed as rotted areas on the middle of the stems. The cortical tissues of the plants showed a distinct brown discoloration. Eventually, the infected plants died. The pathogen was isolated from Dracaena stems and identified as F. solani by a fragment of the translation elongation factor 1-alpha (EF-1α) gene. Fusarium solani was confirmed by a pathogenicity test, and the causal agent was re-isolated from infected D. sanderiana plants. To the best of our knowledge, this is the first report of stem rot caused by F. solani on the cuttings of D. sanderiana.

Botryosphaeria sinensia sp nov., a new species from China

Phytotaxa ◽  
2016 ◽  
Vol 245 (1) ◽  
pp. 43 ◽  
Author(s):  
Yupei Zhou ◽  
Zhipeng Dou ◽  
Wei He ◽  
Xiaodong Zhang ◽  
Ying Zhang
Keyword(s):  
New Species ◽  
Dna Sequence ◽  
Elongation Factor ◽  
Intergenic Spacer ◽  
Translation Elongation Factor ◽  
Sequence Comparisons ◽  
Translation Elongation ◽  
Elongation Factor 1 Alpha ◽  
Elongation Factor 1 ◽  
A New Species

A new Botryosphaeria species (B. sinensia) is described and illustrated from China. It is characterized by erumpent, botryose clustered ascostromata, ostiolate ascomata, bitunicate, 8-spored and clavate asci, filiform, rarely branched pseudoparaphyses, hyaline, 1-celled, fusiform ascospores, and a Fusicoccum asexual morph. The hyaline old and discharged conidia with 1–2 septa, broader spermatia formed in the culture and smaller ascostroma readily distinguished it from B. dothidea and other species in Botryosphaeria. The intergenic spacer (ITS)  and translation elongation factor 1-alpha (EF1) DNA sequence analysis results also support its separation from other Botryosphaeria species. Its relationships with other species of Botryosphaeria are also discussed based on morphology and DNA sequence comparisons.

scholarly journals Morphology and Phylogeny of Gnomoniopsis (Gnomoniaceae, Diaporthales) from Fagaceae Leaves in China

2021 ◽  
Vol 7 (10) ◽  
pp. 792
Author(s):  
Ning Jiang ◽  
Hermann Voglmayr ◽  
Dan-Ran Bian ◽  
Chun-Gen Piao ◽  
Sheng-Kun Wang ◽  
...  
Keyword(s):  
Ribosomal Dna ◽  
Elongation Factor ◽  
Nuclear Ribosomal Dna ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Elongation Factor 1 Alpha ◽  
Its Regions ◽  
Plant Families ◽  
Internally Transcribed Spacer ◽  
Elongation Factor 1

Gnomoniopsis (Gnomoniaceae, Diaporthales) is a well-classified genus inhabiting leaves, branches and fruits of the hosts in three plant families, namely Fagaceae, Onagraceae and Rosaceae. In the present study, eighteen Gnomoniopsis isolates were obtained from diseased leaves of Fagaceae hosts collected from Fujian, Guangdong, Hainan, Henan, Jiangxi and Shaanxi provinces in China. Morphology from the cultures and phylogeny based on the 5.8S nuclear ribosomal DNA gene with the two flanking internally transcribed spacer (ITS) regions, the translation elongation factor 1-alpha (tef1) and the beta-tubulin (tub2) genes were employed to identify these isolates. As a result, seven species were revealed, viz. Gnomoniopsis castanopsidis, G. fagacearum, G. guangdongensis, G. hainanensis, G. rossmaniae and G. silvicola spp. nov, as well as a known species G. daii. In addition, G. daii was firstly reported on the host Quercus aliena.

scholarly journals Translation elongation factor 1-alpha gene as a marker for diagnosing of Candida onychomycosis

2020 ◽  
Author(s):  
Keyvan Pakshir ◽  
Forough Farazmand ◽  
Farnoush Ghasemi ◽  
Hossein Mirhend ◽  
Kamiar Zomorodian ◽  
...  
Keyword(s):  
Candida Species ◽  
Simple Procedure ◽  
Elongation Factor ◽  
Sequence Difference ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Elongation Factor 1 Alpha ◽  
Base Pair Fragment ◽  
Successful Amplification ◽  
Elongation Factor 1

Background and Purpose: Culture-based identification methods have been the gold standard for the diagnosis of candidal onychomycosis. Molecular technologies, such as polymerase chain reaction (PCR) assays, can provide an alternative for the rapid detection of Candida species. The present study was conducted to investigate a pan-Candida PCR assay based on the translation elongation factor 1-alpha (TEF-1α) gene for the detection of the most prevalent pathogenic Candida species. Materials and Methods: For the purpose of the study, an optimized pan-Candida PCR primer pair was designed, and the target was amplified and sequenced. The analytical and clinical diagnostic performance of the designed primers was tested using 17 reference strains, 137 nail scrapings suspected of onychomycosis, and 10 healthy nail specimens. Results: The use of the universal pan-Candida primers designed on TEF-1α gene resulted in the successful amplification of a 270-base pair fragment in all Candida species tested, except for C. glabrata, and reacted neither with other fungi nor with E. coli. The sequence difference count matrix showed poor insertion/deletion differences (0-2 nt) among Candida species. Among 137 nail specimens, 35% (n=48), 30.7% (n=42), and 40.1% (n=55) of the samples were found to be positive by direct microscopy, culture, and pan-Candida PCR, respectively. Conclusion: Based on the findings, the PCR-based detection targeting the DNA TEF-1α gene is a rapid and simple procedure for the diagnosis of candidal onychomycosis directly from nail sample.

Sign in / Sign up

Export Citation Format

Share Document