scholarly journals Importance, Genetic Diversity and Prospects for Varietal Improvement of Ginger (Zingiber officinale Roscoe) in Burkina Faso

2021 ◽  
Vol 9 (3) ◽  
pp. 92-99
Author(s):  
Korotimi Deme ◽  
Moumouni Konate ◽  
Hamed Mahamadi Ouedraogo ◽  
Jacob Sanou ◽  
Mahamadou Sawadogo
Keyword(s):  
Genetic Diversity ◽  
Burkina Faso ◽  
Zingiber Officinale ◽  
Varietal Improvement

Genetic diversity of cowpea (Vigna unguiculata L. Walp) cultivars in Burkina Faso resistant to Striga gesnerioides

2010 ◽  
Vol 9 (48) ◽  
pp. 8146-8153 ◽  
Author(s):  
Sawadogo Mahamadou ◽  
T Ouedraogo Jeremy ◽  
S Gowda Bhavani ◽  
P Timko Michael
Keyword(s):  
Genetic Diversity ◽  
Burkina Faso ◽  
Vigna Unguiculata ◽  
Striga Gesnerioides

Microsatellite analysis to rationalize grape germplasm in India and development of a molecular database

2013 ◽  
Vol 11 (3) ◽  
pp. 225-233 ◽  
Author(s):  
Anuradha Upadhyay ◽  
Lalitkumar B. Aher ◽  
Manisha P. Shinde ◽  
Kavita Y. Mundankar ◽  
Anuj Datre ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Molecular Data ◽  
Computer Application ◽  
Parental Genotype ◽  
Application Software ◽  
Microsatellite Data ◽  
Population Structure Analysis ◽  
Varietal Improvement ◽  
User Friendly

Three hundred and seventeen grape accessions from the National Active Grape Germplasm Site in India were analysed with 25 microsatellite markers. A total of 411 alleles were detected, of which 42% were rare alleles. Unique alleles for 56 genotypes were also identified. The analysis of microsatellite data identified 63 duplicate accessions and only 254 accessions were unique genotypes. Several cases of misnomers, synonymy and homonymy were identified. Parental genotype for a few clonal selections was ascertained. Population structure analysis grouped 254 unique genotypes into four major clusters. The analysis also revealed the presence of admixtures with only 79% of pure ancestry. A core collection comprising 80 genotypes was identified, which represented all the alleles and genetic diversity. A user-friendly and interactive computer application software was developed for storage and the retrieval of molecular data. A molecular database for the 254 genotypes was created. This analysis will help in the rationalization and better management of germplasm. Information on genetic diversity and population structure will form the basis for varietal improvement programmes.

scholarly journals Genetic variation of Indian potato (Solanum tuberosum L.) genotypes using chromosomal and RAPD markers

2010 ◽  
Vol 10 (3) ◽  
pp. 238-246 ◽  
Author(s):  
Anath Bandhu Das ◽  
Iswar Chandra Mohanty ◽  
Dawanidhi Mahapatra ◽  
Suprava Mohanty ◽  
Ashutosh Ray
Keyword(s):  
Genetic Diversity ◽  
Rapd Markers ◽  
Solanum Tuberosum L ◽  
Chromosome Analysis ◽  
Genetic Distances ◽  
Chromosome Length ◽  
Similarity Coefficients ◽  
Varietal Improvement ◽  
And Cluster Analysis ◽  
4C Dna Content

Genetic diversity in thirty Indian potatoes were analyzed employing karyotype, genome size and RAPD markers. Chromosome analysis revealed 2n = 4x = 48 except cv. K. Chandramukhi (2n = 4x + 2 = 50). Total genomic chromosome length and chromosome volume varied from 21.14µm in cv. K. Pukhraj to 31.91µm in cv. MS/89-60 and 14.31µm³ in cv. MS/92-1090 to 33.16µm³ in cv. JW-160 respectively. 4C DNA content was significantly varied from 3.640 pg (~891 Mbp) in cv. MS/92-1090 to 11.12 pg (~2747 Mbp) in cv. K. Chandramukhi. RAPD revealed 131 amplified DNA fragments (300 to 2200 bp) with 79 unique bands (7 to 71% polymorphism) among the genotypes. Similarity coefficients (ranged from 0.29 to 0.93) and cluster analysis reflected the expected trends in relationships of the full and half-sib potato genotypes. Genetic distances obtained from a dendrogram could help breeders to choose the diverse parents for a breeding program aimed at varietal improvement.

Genetic diversity analysis and redundant identification in 48 core collections of Zingiber officinale Rosc. (Zingiberaceae)

2016 ◽  
Vol 39 (3) ◽  
pp. 869-883 ◽  
Author(s):  
Enketeswara Subudhi ◽  
Aradhana Das ◽  
Raj Kumar Joshi ◽  
Sujata Mohanty ◽  
Sanghamitra Nayak
Keyword(s):  
Genetic Diversity ◽  
Zingiber Officinale ◽  
Diversity Analysis ◽  
Core Collections ◽  
Genetic Diversity Analysis

scholarly journals Distribution of msp1, msp2 and eba175 Allelic Family According to Hemoglobin Genotype and G6PD Type from Children with Uncomplicated Malaria in Banfora Heath District (Burkina Faso)

2020 ◽  
pp. 36-47
Author(s):  
Salif Sombié ◽  
Samuel Sindié Sermé ◽  
Aïssatou Diawara ◽  
Mame Massar Dieng ◽  
Amidou Diarra ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Burkina Faso ◽  
Venous Blood ◽  
Malaria Diagnosis ◽  
Allelic Family ◽  
Pcr Products ◽  
Abnormal Hemoglobin ◽  
Age Range ◽  
Msp2 Gene

Aim: The present study aimed to evaluate the Plasmodium falciparum genetic diversity according to the host hemoglobin and G6PD genetic variants during the course of malaria in infected children aged from 2 to 10 years and living in endemic area in Burkina Faso. Study Design: The study was designed as a longitudinal follow up conducted between May 2015 and February 2016 in Banfora health district, Burkina Faso. Methodology: We included 136 subjects (73 males and 63 females; age range from 2-10 years). Blood thick and thin film was done by capillary blood. Venous blood was collected for DNA extraction. Malaria diagnosis was done by microscopy. Human and parasite DNA were extracted based on Qiagen kit procedure. Then, hemoglobin and G6PD were genotyped by RLFP-PCR while the msp1, msp2 and eba175 genes were typed by a nested PCR. All PCR products were analyzed by electrophoresis on a 1.5-2% agarose gel and alleles categorized according to the molecular weight. Results: The prevalence of hemoglobin type was 19.11% for abnormal hemoglobin and 80.9% for normal hemoglobin carriage. The prevalence of G6PD type was 91.18% for normal and 8.82% for G6PD deficiency carriage, respectively. The prevalence of msp1 allelic families was 81.60%, 80.80% and 67.20% for k1, ro33 and mad20 respectively while for msp2 gene, fc27 and 3D7 allelic family the prevalence was 70.53% and 69.64% respectively. The eba175 allelic families’ distribution showed 77.31% and 40.21% for fcr3 and Camp respectively. There was no difference in multiplicity of infection (MOI) according to hemoglobin genotypes and G6PD types. We found that k1 was the predominant allelic family of msp1 in normal hemoglobin genotype (AA) and normal G6PD type. The mixed infection of eba175 was statistically higher in abnormal hemoglobin (p=0.04). There was no statistical difference between fcr3 and camp prevalence excepted in G6PD deficient type. The polymorphism results showed that the prevalence of 450 bp in fc27 was statistically significantly higher in normal hemoglobin variant carriers (AA) than abnormal hemoglobin carriers (p=2.10 -4)). However, the prevalence of 350 bp in fc27 was statistically higher in normal G6PD than deficient G6PD carriers (p=0.034). Conclusion: Our result showed that the distribution of msp1 and eba75 polymorphism could be influenced by hemoglobin and G6PD variants. These results suggest that hemoglobin and G6PD could influence P. falciparum genetic diversity.

scholarly journals Genetic Structure and Diversity of Rice From Certified Cultivars and Farmer’s Fields in Burkina Faso

2021 ◽  
Author(s):  
Mariam BARRO ◽  
Abdourasmane KONATE ◽  
Issa WONNI ◽  
Abalo Itolou KASSANKOGNO ◽  
François SABOT ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Oryza Sativa ◽  
West Africa ◽  
Burkina Faso ◽  
Snp Array ◽  
Genetic Data ◽  
Genetic Group ◽  
Oryza Glaberrima ◽  
Rainfed Lowland ◽  
Rice Samples

Abstract Background: In West Africa, two rice species are cultivated, the African rice Oryza glaberrima and the Asian rice Oryza sativa, the second one being much more frequent. Despite its importance for food security in West Africa, the genetic diversity of Oryza sativa in farmer’s fields remains understudied in this region. Documenting the dynamics of diversity in the fields between landraces and improved cultivars is of importance to ensure rice cultivation adaptation to global change.In this study, we genotyped 77 rice samples from Burkina Faso using the C6AIR SNP array from IRRI. Among the studied samples, 27 were certified cultivars and 50 were sampled in rice fields from three geographical zones in western Burkina Faso, each zone comprising one irrigated area and a neighboring rainfed lowland. Obtained data were analyzed in the frame of the worldwide rice genetic diversity by using the 3K genomes as reference.Results: Most of the certified cultivars clustered with the indica genetic group, with a minority (26%) assigned to the japonica group. All except one of the rice samples from farmer’s fields belonged to the indica group. The peculiar one unexpectedly clustered with the Aus genetic group and originated from Tengrela (rainfed lowland in Karfiguela zone). This site, already known to differ in terms of agronomic practices, showed the highest genetic diversity compared to the five other sites, as well as highest genetic differentiation. Obtained genetic data confirmed the high global frequency of one cultivar, in accordance with the data from farmer’s interview. However, at the field level, genetic data rarely corresponded to the cultivar’s names obtained from farmer’s interviews.Conclusions: Overall we found a high genetic diversity in the studied samples from Burkina Faso (certified and field’s samples). We argue on the importance to document and preserve this agro-biodiversity as a prerequisite to face the current challenges of growing rice demand and global change. To this purpose, are required further scientific studies to refine our understanding of the dynamics of diversity in farmers’ fields, as well as a better knowledge of rice agro-biodiversity and awareness of its importance by rice farmers themselves.

scholarly journals Development of EST-SSR markers based on transcriptome and its validation in ginger (Zingiber officinale Rosc.)

PLoS ONE ◽  
2021 ◽  
Vol 16 (10) ◽  
pp. e0259146
Author(s):  
Venugopal Vidya ◽  
Duraisamy Prasath ◽  
Mohandas Snigdha ◽  
Ramasamy Gobu ◽  
Charles Sona ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Ssr Markers ◽  
Expressed Sequence Tag ◽  
Zingiber Officinale ◽  
Eastern India ◽  
Coding Sequences ◽  
North Eastern ◽  
Ssr Primers ◽  
Ssr Loci ◽  
North Eastern India

Ginger (Zingiber officinale Rosc.) is an economically important and valuable spice crop around the world. It is used as food, spice, condiment, and medicine. A considerable extent of genetic diversity in ginger occurs in the Western Ghats and North-Eastern India. However, genetic diversity studies at the molecular level in ginger is limited due to limited availability of genetic and genomic information. In the present study, for the first time, we have identified and validated expressed sequence tag (EST)-simple sequence repeat (SSR) markers from ginger. We obtained 16,790 EST-SSR loci from 78987 unigenes, and 4597 SSR loci in the predicted 76929 coding sequences from RNA-Seq assembled contigs of ginger through Illumina paired-end sequencing. Gene ontology results indicate that the unigenes with SSR loci participate in various biological processes such as metabolism, growth, and development in ginger. One hundred and twenty-five primer pairs were designed from unigenes and coding sequences. These primers were tested for PCR optimization, characterization, and amplification and identified 12 novel EST-SSR markers. Twelve flanking polymorphic EST-SSR primers were validated using 48 ginger genotypes representing North-Eastern India and different eco-geographical adaptations by PCR amplification and allele sizing through capillary electrophoresis. Twelve EST-SSR primers generated a total of 111 alleles with an average of 9.25 alleles per locus and allele sizes ranging between 115-189bp. This study implies that the SSR markers designed from transcriptome sequences provides ample EST-SSR resources, which are helpful for genetic diversity analysis of Zingiberaceae species and molecular verification of ginger genotypes.

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