Bunketsu-waito, One of the Tillering Dwarfs, is Controlled by a Single Recessive Gene in Rice (Oryza sativa L.)

Hull opening is a key physiological process during reproductive development, strongly affecting the subsequent fertilization and seed development in rice. In this study, we characterized a rice mutant, non-open hull 1 (noh1), which was derived from ethylmethane-sulfonate (EMS)-treated Xinong 1B (Oryza sativa L.). All the spikelets of noh1 developed elongated and thin lodicules, which caused the failure of hull opening and the cleistogamy. In some spikelets of the noh1, sterile lemmas transformed into hull-like organs. qPCR analysis indicated that the expression of A- and E-function genes was significantly upregulated, while the expression of some B-function genes was downregulated in the lodicules of noh1. In addition, the expression of A-function genes was significantly upregulated, while the expression of some sterile-lemma maker genes was downregulated in the sterile lemma of noh1. These data suggested that the lodicule and sterile lemma in noh1 mutant gained glume-like and lemma-like identity, respectively. Genetic analysis showed that the noh1 trait was controlled by a single recessive gene. The NOH1 gene was mapped between the molecular markers ZJ-9 and ZJ-25 on chromosome 1 with a physical region of 60 kb, which contained nine annotated genes. These results provide a foundation for the cloning and functional research of NOH1 gene.

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Temperature stress on the expression of a single recessive gene controlling internode elongation in rice plants (Oryza sativa L.).

Environment Control in Biology ◽

10.2525/ecb1963.24.59 ◽

1986 ◽

Vol 24 (2) ◽

pp. 59-66

Author(s):

Norindo TAKAHASHI ◽

Tadashi SATO

Keyword(s):

Oryza Sativa ◽

Temperature Stress ◽

Oryza Sativa L ◽

Recessive Gene ◽

Internode Elongation ◽

Single Recessive Gene ◽

Rice Plants

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INHERITANCE OF FERTILITY RESTORATION INVOLVING WILD ABORTIVE CYTOPLASMIC MALE STERILITY SYSTEM IN RICE (Oryza sativa L.)

Bangladesh Journal of Plant Breeding and Genetics ◽

10.3329/bjpbg.v24i1.16997 ◽

2011 ◽

Vol 24 (1) ◽

pp. 33-40

Author(s):

M. J. Hasan ◽

M. U. Kulsum ◽

A. Ansari ◽

A. K. Paul ◽

P. L. Biswas

Keyword(s):

Oryza Sativa ◽

Cytoplasmic Male Sterility ◽

Male Sterility ◽

Gene Action ◽

Oryza Sativa L ◽

Fertility Restoration ◽

Dominant Gene ◽

Recessive Gene ◽

Male Sterile ◽

Male Sterile Line

Inheritance of fertility restoration was studied in crosses involving ten elite restorer lines of rice viz. BR6839-41-5-1R, BR7013-62-1-1R, BR7011-37-1-2R, BR10R, BR11R, BR12R, BR13R, BR14R, BR15R and BR16R and one male sterile line Jin23A with WA sources of cytoplasmic male sterility. The segregation pattern for pollen fertility of F2 and BC1 populations of crosses involving Jin23A indicated the presence of two independent dominant fertility restoring genes. The mode of action of the two genes varied in different crosses revealing three types of interaction, i.e. epistasis with dominant gene action, epistasis with recessive gene action, and epistasis with incomplete dominance.DOI: http://dx.doi.org/10.3329/bjpbg.v24i1.16997

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Physiological characterisation and fine mapping of a salt-tolerant mutant in rice (Oryza sativa)

Functional Plant Biology ◽

10.1071/fp15126 ◽

2015 ◽

Vol 42 (11) ◽

pp. 1026 ◽

Cited By ~ 11

Author(s):

Ping Deng ◽

Dan Jiang ◽

Yanmin Dong ◽

Xingyu Shi ◽

Wen Jing ◽

...

Keyword(s):

Oryza Sativa ◽

Salt Stress ◽

Salt Tolerance ◽

Oryza Sativa L ◽

Recessive Gene ◽

Nacl Stress ◽

Shoot Biomass ◽

Salt Tolerant ◽

Candidate Locus ◽

F2 Population

Salt-tolerant mutants are valuable resources for basic and applied research on plant salt tolerance. Here, we report the isolation and characterisation of a salt-tolerant rice (Oryza sativa L.) mutant. This mutant was identified from an ethyl methanesulfonate-induced Nipponbare mutant library, designated as rice salt tolerant 1 (rst1). The rst1 mutant was tolerant to salt stress and showed significantly higher shoot biomass and chlorophyll content, but lower lipid peroxidation and electrolyte leakage under NaCl stress. The improved salt tolerance of this mutant may be due mainly to its enhanced ability to restrict Na+ accumulation in shoots under salt stress conditions. Genetic analysis indicated that the salt tolerance of the rst1 mutant was controlled by a single recessive gene. Quantitative trait locus (QTL) mapping for salt tolerance was performed using an F2 population of rst1 × Peiai 64. Two QTLs were detected, in which the locus on chromosome 6 was determined to be the candidate locus of the rst1 gene. The rst1 locus was subsequently shown to reside within a 270.4-kb region defined by the markers IM29432 and IM29702. This result will be useful for map-based cloning of the rst1 gene and for marker-assisted breeding for salt tolerance in rice.

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Genetic control of meiosis in rice, Oryza sativa L. IV. Cytogenetical analyses of asynaptic mutants

Canadian Journal of Genetics and Cytology ◽

10.1139/g84-042 ◽

1984 ◽

Vol 26 (3) ◽

pp. 264-271 ◽

Cited By ~ 3

Author(s):

Kunio Kitada ◽

Takeshi Omura

Keyword(s):

Oryza Sativa ◽

Genetic Control ◽

Chromosome Pairing ◽

Chiasma Frequency ◽

Oryza Sativa L ◽

Recessive Gene ◽

The Other ◽

Normal Plant ◽

Meiotic Stage ◽

The Mean

One complete asynaptic mutant, MM-19, and two partial ones, MM-4 and MM-16, of Oryza sativa L. induced by N-methyl-N-nitrosourea (MNU) were cytogenetically investigated. No chromosome pairing occurred from zygotene to pachytene and 24 univalents appeared at diakinesis and metaphase 1 in MM-19. On the other hand, a partial lack of chromosome pairing was observed from zygotene to pachytene and various numbers of univalents occurred at metaphase I in MM-4 and MM-16. The mean chiasma frequency per bivalent as well as per cell decreased to different extents in MM-4 and MM-16, and the correlation between both the amount of chromosome pairing from zygotene to pachytene and the chiasma frequency per cell at diakinesis was recognized. Judging from the development of anthers in each meiotic stage, the duration of the stage forming the synizetic knot, at which chromosome pairing took place, was longer in MM-4 and MM-16 than in the normal plant, and was in MM-19 almost as long as in the normal plant. The results of gene analyses indicate that each of the three asynaptic mutants is controlled by a recessive gene and that, at least for MM-4 and MM-16, these genes are located at different loci.Key words: asynaptic, rice, Oryza, chiasma frequency, synizesis.

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Fine mapping of an incom-plete recessive gene for leaf rolling in rice (Oryza sativa L.)

Chinese Science Bulletin ◽

10.1360/982005-999 ◽

2005 ◽

Vol 50 (21) ◽

pp. 2466 ◽

Cited By ~ 3

Author(s):

Yuanjian SHAO

Keyword(s):

Oryza Sativa ◽

Fine Mapping ◽

Oryza Sativa L ◽

Recessive Gene ◽

Leaf Rolling

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Fine mapping of a pistilloid-stamen (PS) gene on the short arm of chromosome 1 in rice

Genome ◽

10.1139/g06-056 ◽

2006 ◽

Vol 49 (8) ◽

pp. 1016-1022 ◽

Cited By ~ 7

Author(s):

Hongfa Luo ◽

Yunfeng Li ◽

Zhenglin Yang ◽

Bingqiang Zhong ◽

Rong Xie ◽

...

Keyword(s):

Oryza Sativa ◽

Simple Sequence Repeat ◽

Molecular Mapping ◽

Oryza Sativa L ◽

Recessive Gene ◽

Physical Region ◽

Floral Organ ◽

Chromosome 1 ◽

Sequence Repeat ◽

Simple Sequence

A novel floral organ mutant of rice (Oryza sativa L. subsp. indica), termed pistilloid-stamen (ps) here, has flowers with degenerated lemma and palea, with some stamens transformed into pistils and pistil–stamen chimeras. Genetic analysis confirmed that the ps trait is controlled by a single recessive gene. F2 and F3 segregation populations derived from PS ps heterozygote crossed with Oryza sativa subsp. indica 'Luhui-17' (PS PS) were used for molecular mapping of the gene using simple sequence repeat (SSR) markers. With 97 recessive individuals from an F2 segregation population, the ps locus was preliminarily mapped 6.2 cM distal to marker RM6324 and 3.1 cM proximal to marker RM6340 in the terminal region of the short arm of chromosome 1. With a large F3 segregation population, the gene was fine-mapped between markers RM6470 and RM1141, at distances of 0.10 and 0.03 cM to each marker, respectively. The position of the ps gene was finally located within a 20 kb physical region containing 3 annotated putative genes. One of them, encoding a protein with a single C2H2 zinc-finger domain, may be the candidate gene for PS.Key words: rice (Oryza sativa L. subsp. indica), pistilloid-stamen mutant (ps mutant), molecular marker, simple sequence repeat (SSR), gene mapping

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Population Structure and Its Effect on Haplotype Diversity and Linkage Disequilibrium Surrounding the xa5 Locus of Rice (Oryza sativa L.)

Genetics ◽

10.1093/genetics/165.2.759 ◽

2003 ◽

Vol 165 (2) ◽

pp. 759-769 ◽

Cited By ~ 1

Author(s):

Amanda J Garris ◽

Susan R McCouch ◽

Stephen Kresovich

Keyword(s):

Population Structure ◽

Oryza Sativa ◽

Linkage Disequilibrium ◽

Sequence Data ◽

Oryza Sativa L ◽

Haplotype Diversity ◽

Recessive Gene ◽

Candidate Region ◽

Linkage Disequilibrium Mapping ◽

Cloned Gene

Abstract To assess the usefulness of linkage disequilibrium mapping in an autogamous, domesticated species, we have characterized linkage disequilibrium in the candidate region for xa5, a recessive gene conferring race-specific resistance to bacterial blight in rice. This trait and locus have good mapping information, a tractable phenotype, and available sequence data, but no cloned gene. We sampled 13 short segments from the 70-kb candidate region in 114 accessions of Oryza sativa. Five additional segments were sequenced from the adjacent 45-kb region in resistant accessions to estimate the distance at which linkage disequilibrium decays. The data show significant linkage disequilibrium between sites 100 kb apart. The presence of the xa5 resistant reaction in two ecotypes and in accessions with different haplotypes in the candidate region may indicate multiple origins or genetic heterogeneity for resistance. In addition, genetic differentiation between ecotypes emphasizes the need for controlling for population structure in the design of linkage disequilibrium studies in rice.

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Inheritance of fertility restoration involving id type cytoplasmic male sterility system in rice (Oryza sativa L.) using ten different restorer lines

SAARC Journal of Agriculture ◽

10.3329/sja.v13i1.24193 ◽

2015 ◽

Vol 13 (1) ◽

pp. 207-215

Author(s):

MJ Hasan ◽

U Kulsum ◽

NE Elahi ◽

AKM Shamsuddin ◽

MM Rahman

Keyword(s):

Oryza Sativa ◽

Cytoplasmic Male Sterility ◽

Male Sterility ◽

Gene Action ◽

Oryza Sativa L ◽

Fertility Restoration ◽

Dominant Gene ◽

Recessive Gene ◽

Male Sterile ◽

Male Sterile Line

Inheritance of fertility restoration was studied in crosses involving ten elite restorer lines of rice viz. BR827R, BR168R, BR6723-1-1-2R, M.H.63R, M.H.77R, Gui99R, IR40750R, IR64R, AjayaR and IR44675R and one male sterile line II32A with ID (Indonesian paddy type) sources of cytoplasmic male sterility. The segregation pattern for pollen fertility of F2 and BC1 populations of crosses involving II32A indicated the presence of two independent dominant fertility restoring genes. The mode of action of the two genes varied in different crosses revealing three types of interaction, i.e. epistasis with dominant gene action, epistasis with recessive gene action, and epistasis with incomplete dominance.SAARC J. Agri., 13(1): 207-215 (2015)

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