Assessment of the congruity of genetic relationships and variation revealed by individual- and bulked-samples-based approaches using RAPD and ISSR markers in Japanese turnip (Brassica rapa ssp. rapa) cultivars

In this study the genetic similarity among Dasypyrum, Secale andTriticum species with RAPDs and ISSRs was analyzed. To show a level of similarity between the species, 12 populations of Dasypyrum (11 D. villosum and 1 D. breviaristatum), together with 12 accessions belonging to 3 Secale species and 12 accessions from 4 Triticum species were used. Genetic distances (GD) and bootstrap values were calculated and PCA analysis was conducted to present the relationships among the species. To estimate the genetic structure among and inside genera, as well as population differentiation, gene diversity (He), total genetic variation (Ht) and Wrightâ€™s fixation index (Fst) were computed. The highest values were found in Triticum, within which Ht was equal to 0.332Â±0.023 and Fst was 0.42. It confirmed that the material studied was highly differentiated. Both systems found Dasypyrum more related with Triticum, as compared to Secale. With RAPDs, genetic distance (GD) between Triticum and Dasypyrum was 0.435, respectively 0.460 for Secale vs Dasypyrum. In the case of ISSRs these values were 0.374 and 0.407, respectively. Despite the fact that the difference between the two GD indices was insignificant, one should not exclude the possibility of successful hybridization of Dasypyrum and Secale, especially when using bridge species.

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Application of RAPD and ISSR markers to analyse molecular relationships in Grevillea (Proteaceae)

Australian Systematic Botany ◽

10.1071/sb03016 ◽

2004 ◽

Vol 17 (1) ◽

pp. 49 ◽

Cited By ~ 22

Author(s):

M. Pharmawati ◽

G. Yan ◽

I. J. McFarlane

Keyword(s):

Phylogenetic Trees ◽

Genetic Relationships ◽

Initial Point ◽

Morphological Characteristics ◽

Issr Markers ◽

Group Method ◽

Pair Group ◽

Molecular Relationships ◽

Group B ◽

Rapd And Issr

The potential of RAPD and ISSR markers to construct molecular relationships of Grevillea was evaluated with 23 RAPD and 12 ISSR primers. The 16 genotypes representing 12 species and 3 subspecies of Grevillea were sampled from the collection of the Mt Anann Botanic Garden, NSW. RAPD and ISSR assays generated a total of 401 RAPD and 280 ISSR fragments. High frequencies of polymorphisms, 99.39% for RAPD and 99.51% for ISSR, were detected by both markers. Three statistical approaches were employed to construct phylogenetic relationships from combined RAPD and ISSR data. Cluster analysis by the unweighted pair group method (UPGMA) of Jaccard's similarity and Neighbour-Joining analysis of total character difference generated dendograms with similar topology. Parsimony analysis also generated a tree that was in broad agreement with the two dendograms. The phylogenetic trees divided the Grevillea species studied into three groups. Group A consisted of G. buxifolia subsp. buxifolia, G. phylicoides and G. sphacelata. In group B, G. mucronulata was grouped together with G. montana, while G. diffusa, G. humilis, G. linearifolia, G. molyneuxii, G. oldei, G. sericea and G. speciosa formed group C. This molecular result was comparable to groupings suggested by a previous author (Makinson 2000) based on morphological characteristics. However, in contrast to the morphological taxonomy, molecular phylogeny suggests that G. oldei and G. speciosa belong to the same subgroup sensu Makinson (2000), whereas G. linearifolia and G. molyneuxii should not be placed in their originally suggested subgroups sensu Makinson (2000). The present study is the first published report on molecular relationships of Grevillea and can be considered as an initial point for further research on the genetic relationships and evolution of Grevillea.

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Efficiency of RAPD and ISSR Markers for the Detection of Polymorphisms and Genetic Relationships in Date Palm

Biotechnology(Faisalabad) ◽

10.3923/biotech.2017.19.26 ◽

2016 ◽

Vol 16 (1) ◽

pp. 19-26 ◽

Cited By ~ 4

Author(s):

Khaled Elmeer ◽

Mai Alghanem ◽

Latifa Al-Latifi ◽

Hayat Alhemairi

Keyword(s):

Date Palm ◽

Genetic Relationships ◽

Issr Markers ◽

Rapd And Issr

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GENETIC RELATIONSHIPS AMONG ERIOBOTRYA PRINOIDES VAR. DADUNENSIS, ERIOBOTRYA PRINOIDES AND ERIOBOTRYA JAPONICA USING RAPD AND ISSR MARKERS

Acta Horticulturae ◽

10.17660/actahortic.2011.887.7 ◽

2011 ◽

pp. 59-64 ◽

Cited By ~ 1

Author(s):

N. Luo ◽

Y.Q. Wang ◽

Y. Fu ◽

Q. Yang

Keyword(s):

Genetic Relationships ◽

Issr Markers ◽

Eriobotrya Japonica ◽

Rapd And Issr

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Determination of genetic diversity among some almond accessions

Genetika ◽

10.2298/gensr1501013p ◽

2015 ◽

Vol 47 (1) ◽

pp. 13-22 ◽

Cited By ~ 1

Author(s):

Hasan Pinar ◽

Sezai Ercisli ◽

Mustafa Unlu ◽

Mustafa Bircan ◽

Aydın Uzun ◽

...

Keyword(s):

Genetic Diversity ◽

Cultivar Identification ◽

Genetic Relationships ◽

Inter Simple Sequence Repeat ◽

Issr Markers ◽

Geographical Isolation ◽

Fruit Species ◽

Rapd And Issr ◽

Simple Sequence

More recently the use of different molecular markers in fruit species to determine particularly genetic diversity, genetic relationships and cultivar identification has been gained more importance. In the study, 13 randomly amplified polimorfic DNA (RAPD) and 4 inter-simple sequence repeat (ISSR) markers were used to evaluate genetic relationships among 95 almong accessions (26 foreign cultivars and 69 national cultivars and selections). The all plant material found in Almond Germplasm Repository in Gaziantep, Turkey. Both RAPD and ISSR markers distinguished the almond cultivars and selections in various levels. 17 RAPD and ISSR markers yielded a total of 73 scorable bands, which 51 are polymorphic. The two marker system exhibited variation with regard to average band sizes and polymorphism ratio. The average polymorphism was higher in ISSR (88%) compared to RAPD (74%). RAPD and ISSR marker systems were found to be useful for determining genetic diversity among almong genotypes and cultivars. Combining of two dendrograms obtained through these markers show different clustering of 96 almond specimens without geographical isolation. These results supported that almonds in Turkey indicated considerable genetic diversity.

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