Linkage groups of field resistance genes of upland rice variety "Sensho" to leaf blast caused by Pyricularia oryzae Cav.

Rice blast caused by Pyricularia oryzae (P. oryzae) is one of the most serious diseases infecting rice worldwide. In the present study, virulence pattern of six P. oryzae pathotypes (P0.0, P0.2, P1.0, P3.0, P7.0 and P9.0) identified from the blast pathogen collected in Peninsular Malaysia, were evaluated using a set of 22 IRRI-bred blast resistance lines (IRBL) as well as to determine the resistance genes involved. The information on the virulence of the blast pathotypes and the resistance genes involved is important for breeding of new rice variety for durable resistance against blast disease. The IRBL was established from 22 monogenic lines, harbouring 22 resistance genes [Pia, Pib, Pii, Pit, Pi3, Pi5(t), Pish, Pi1, Pik, Pik-s, Pik-m, Pik-h, Pik-p, Pi7(t), Pi9, Piz, Piz-5, Piz-t, Pi19, Pi20(t), Pita-2, and Pita=Pi4(t)]. Based on the disease severity patterns, the tested pathotypes were avirulence towards seven IRBLs [IRBLi-F5, IRBLk-Ka, IRBLkh-K3, IRBLz-Fu, IRBLsh-S, IRBLPi7 (t) and IRBL9-W] of which these IRBLs harbouring Pii, Pik, Pik-h, Piz, Pish, Pi7(t) and Pi9 resistance genes, respectively. Therefore, the results suggested that the seven IRBLs carrying seven resistance genes [Pii, Pik, Pik-h, Piz, Pish, Pi7(t) and Pi9] would be suitable candidates of resistance genes to be incorporated in new breeding lines to combat the current blast pathotypes in the field.

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Keparahan Penyakit Blas Pyricularia oryzae dan Analisis Gen Virulensi Menggunakan Metode Sequence Characterized Amplified Region

Jurnal Fitopatologi Indonesia ◽

10.14692/jfi.17.1.19-27 ◽

2021 ◽

Vol 17 (1) ◽

pp. 19-27

Author(s):

Gilang Kurrata ◽

Tutik Kuswinanti ◽

Andi Nasruddin

Keyword(s):

Genetic Diversity ◽

Evaluation System ◽

Rice Variety ◽

Dominant Gene ◽

Pyricularia Oryzae ◽

Blast Disease ◽

Leaf Blast ◽

Sequence Characterized Amplified Region ◽

Pesticide Pollution ◽

Resistant Varieties

Severity of Blast Disease and Analysis of Virulence Gene Using Sequence Characterized Amplified Region Method Rice blast disease caused by the fungus Pyricularia oryzae (teleomorph: Magnaporthe oryzae) is one of the important diseases in rice cultivation in the world, including Indonesia. The use of resistant varieties is a way to deal with blast disease since it is cheap, efficient and safe from the risk of pesticide pollution. However, the resistance of a rice variety to blast disease can only be utilized for a few years due to the complexity of pathogens which can easily break down the resistance of a variety, especially if their resistance is determined only by one dominant gene. The aims of this research was to determine the severity of blast disease and genetic variation of P. oryzae isolates from Maros District and the relationship between disease severity and haplotype variety obtained as the basis for recommendations for control with site-specific resistant varieties. The severity of blast disease was observed using the Standard Evaluation System for Rice was carried out in 8 locations (different sub-districts) in Maros District, South Sulawesi from June to September 2019. The pathogen’s genetic diversity was determined using specific primers, coding the fungus virulence genes namely Pwl2, Erg2 and Cut1. The highest leaf blast intensity was observed on var. Mekongga in Simbang sub-district (42.12%) and Maros Baru sub-district (23.33%). In Tanralili sub-district (Inpari-7) and Mandai sub-district (var. Ciherang) leaf blast intencity were 7.6% and 7.88% respectively. A total of 15 isolates of P. oryzae obtained from 8 sub-districts in Maros. Analysis of genetic diversity showed found 5 different haplotypes, namely haplotype A-000 (4 isolates), C-011 (3 isolates), D-111 (2 isolates), F-110 (3 isolates) and G-100 (3 isolates).

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Mapping QTLs for Field Resistance to Rice Blast in the Japanese Upland Rice Variety Norin 12

Breeding Science ◽

10.1270/jsbbs.56.415 ◽

2006 ◽

Vol 56 (4) ◽

pp. 415-418 ◽

Cited By ~ 13

Author(s):

Hiroyuki Sato ◽

Yoshinobu Takeuchi ◽

Hideyuki Hirabayashi ◽

Hiroshi Nemoto ◽

Masakata Hirayama ◽

...

Keyword(s):

Rice Blast ◽

Upland Rice ◽

Rice Variety ◽

Field Resistance

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Intensitas Penyakit Blas (Pyricularia oryzae Cav.) pada Padi Varietas Ciherang di Lokasi Endemik dan Pengaruhnya terhadap Kehilangan Hasil

Agrikultura ◽

10.24198/agrikultura.v27i3.10878 ◽

2016 ◽

Vol 27 (3) ◽

Cited By ~ 3

Author(s):

Tarkus Suganda ◽

Endah Yulia ◽

Fitri Widiantini ◽

Hersanti Hersanti

Keyword(s):

Yield Loss ◽

Block Design ◽

Rice Variety ◽

Pyricularia Oryzae ◽

Control Measures ◽

Blast Disease ◽

Management Policy ◽

Potential Yield ◽

Leaf Blast ◽

Neck Blast

ABSTRACTDisease intensity of blast disease (Pyricularia oryzae Cav.) of Ciherang rice variety at the endemic location and its effect on yield lossBlast is one of the most important diseases of rice worldwide. Many countries have developed data on the intensity and yield loss due to blast disease, whereas Indonesia has no such data resulted from trial specifically designed to estimate blast intensity at the endemic location and its potential yield loss. Such data are needed for various purposes, such as for disease management policy and research background. A trial has been carried out at Desa Bojong, Kecamatan Cikembar, Kabupaten Sukabumi, a location where blast was endemic for years, using var. Ciherang that is known as susceptible and widely grown by farmers. Eight concentrations of fungicide of single as well as mix active ingredients were used to see the effects in suppressing blast diseases compared with of control. Trial used a Randomized Block Design with 5 replicates. The parameters observed were the intensity of leaf blast, neck blast and yield. The results showed that the disease intensity of var. Ciherang at the endemic location was 55.60% of leaf blast and 37.75% of neck blast. The potency of yield loss calculated was 3.65 ton/ha or equal with 61% of the average yield of var. Ciherang as described in its variety description. This number justifies the important of control measures of rice blast diseases.Keywords: Blast disease intensity, var. Ciherang, Potency of yield lossABSTRAKPenyakit blas merupakan salah satu penyakit utama pada tanaman padi di seluruh dunia. Berbagai negara sudah memiliki data tentang intensitas dan kehilangan hasil padi oleh penyakit blas, sementara di Indonesia belum ada hasil pengujian yang khusus dirancang untuk melihat tingkat intensitas penyakit blas di daerah endemik dan potensi kehilangan hasil yang diakibatkannya. Data sejenis ini penting untuk berbagai keperluan antara lain untuk kebijakan pengendalian dan dasar pentingnya penelitian. Suatu percobaan telah dilakukan di Desa Bojong, Kecamatan Cikembar, Kabupaten Sukabumi yang merupakan daerah endemik penyakit blas selama bertahun-tahun, menggunakan var Ciherang yang merupakan varietas rentan namun populer ditanam petani. Delapan bahan aktif fungisida berbahan aktif tunggal dan majemuk digunakan untuk melihat pengaruh penekanannya dibandingkan kontrol. Percobaan menggunakan Rancangan Acak Kelompok dengan lima ulangan. Parameter yang diamati adalah intensitas penyakit blas daun dan blas leher malai serta hasil padi. Hasil percobaan menunjukkan bahwa intensitas penyakit blas pada tanaman padi var. Ciherang di daerah endemik adalah 55,60% untuk blas daun, dan 37,75% untuk blas leher malai. Potensi kehilangan hasil oleh gabungan penyakit blas pada var. Ciherang adalah 3,65 ton/ha atau setara dengan 61% kehilangan hasil jika dibandingkan terhadap rata-rata produksi var. Ciherang menurut spesifikasi varietas. Data ini menjustifikasi perlunya pengambilan tindakan pengendalian penyakit blas pada tanaman padi.Kata Kunci: Intensitas penyakit blas, var. Ciherang, Potensi kehilangan hasil

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Mapping of Quantitative Trait Loci Conferring Blast Field Resistance in the Japanese Upland Rice Variety Kahei.

Breeding Science ◽

10.1270/jsbbs.51.257 ◽

2001 ◽

Vol 51 (4) ◽

pp. 257-261 ◽

Cited By ~ 32

Author(s):

Masaru Miyamoto ◽

Masahiro Yano ◽

Hideo Hirasawa

Keyword(s):

Quantitative Trait Loci ◽

Quantitative Trait ◽

Upland Rice ◽

Rice Variety ◽

Field Resistance ◽

Trait Loci

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Origin of host-specificity resistance genes of common wheat against non-adapted pathotypes of Pyricularia oryzae inferred from D-genome diversity in synthetic hexaploid wheat lines

Journal of General Plant Pathology ◽

10.1007/s10327-021-00990-2 ◽

2021 ◽

Author(s):

Yoshihiro Inoue ◽

Trinh Thi Phuong Vy ◽

Soichiro Asuke ◽

Yoshihiro Matsuoka ◽

Yukio Tosa

Keyword(s):

Host Specificity ◽

Common Wheat ◽

Resistance Genes ◽

Hexaploid Wheat ◽

Pyricularia Oryzae ◽

Synthetic Hexaploid Wheat ◽

Genome Diversity ◽

D Genome ◽

Synthetic Hexaploid ◽

Wheat Lines

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Dissection of broad-spectrum resistance of the Thai rice variety Jao Hom Nin conferred by two resistance genes against rice blast

Rice ◽

10.1186/s12284-017-0159-0 ◽

2017 ◽

Vol 10 (1) ◽

Cited By ~ 12

Author(s):

Chaivarakun Chaipanya ◽

Mary Jeanie Telebanco-Yanoria ◽

Berlaine Quime ◽

Apinya Longya ◽

Siripar Korinsak ◽

...

Keyword(s):

Resistance Genes ◽

Broad Spectrum ◽

Rice Blast ◽

Rice Variety ◽

Broad Spectrum Resistance

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Evaluation of Short Duration Drought Tolerant Rice Varieties in Drought Prone Areas of Subarnapur District of Odisha

International Journal of Plant & Soil Science ◽

10.9734/ijpss/2020/v32i830314 ◽

2020 ◽

pp. 21-26

Author(s):

S. Pradhan ◽

F. H. Rahman ◽

S. Sethy ◽

G. Pradhan ◽

J. Sen

Keyword(s):

Short Duration ◽

Upland Rice ◽

Plant Protection ◽

Block Design ◽

Rice Variety ◽

Rice Varieties ◽

Agronomic Practices ◽

Drought Tolerant ◽

Randomized Block Design ◽

Sowing Seed

A study was conducted in farmer’s field through technology demonstrations during Kharif season of 2018 and 2019 in Subarnapur district of Odisha to recommend the most suited short duration drought tolerant upland rice variety particular to the locality. Six different varieties like Sahabhagi dhan, Mandakini, Jogesh, DRR-44, and DRR-42 & Khandagiri were taken with four replications in Randomized Block Design in both years. Technology demonstration included not only the improved short duration varieties, but also a package of good agronomic practices viz. line sowing, seed treatment with Bavistin, proper fertilization with dose @ 80:40:40 NPK/ha, pre emergence herbicide application with pretilachlor, needful manual weeding and need based plant protection chemicals. The results of demonstration showed that farmers could increase rice productivity notably by adopting improved variety Sahabhagi dhan and DRR-44. These varieties recorded higher grain yields of 64% and 46%, respectively, and fetched a sum return of Rs 25620 and Rs 25275 over farmers’ local variety Khandagiri.

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