Cystatin C in Milk Basic Protein (MBP) and Its Inhibitory Effect on Bone Resorptionin Vitro

Myelination in the central nervous system provides a unique example of how cells establish asymmetry. The myelinating cell, the oligodendrocyte, extends processes to and wraps multiple axons of different diameter, keeping the number of wraps proportional to the axon diameter. Local regulation of protein synthesis represents one mechanism used to control the different requirements for myelin sheath at each axo–glia interaction. Prior work has established that β1-integrins are involved in the axoglial interactions that initiate myelination. Here, we show that integrin activation regulates translation of a key sheath protein, myelin basic protein (MBP), by reversing the inhibitory effect of the mRNA 3′UTR. During oligodendrocyte differentiation and myelination α6β1-integrin interacts with hnRNP-K, an mRNA-binding protein, which binds to MBP mRNA and translocates from the nucleus to the myelin sheath. Furthermore, knockdown of hnRNP-K inhibits MBP protein synthesis during myelination. Together, these results identify a novel pathway by which axoglial adhesion molecules coordinate MBP synthesis with myelin sheath formation.

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Milk basic protein increases ghrelin secretion and bone mineral density in rodents

Nutrition ◽

10.1016/j.nut.2017.02.003 ◽

2017 ◽

Vol 39-40 ◽

pp. 15-19 ◽

Cited By ~ 2

Author(s):

Yuko Ishida ◽

Rakhi Chacrabati ◽

Aiko Ono-Ohmachi ◽

Zhi Gong ◽

Chika Ikenoya ◽

...

Keyword(s):

Bone Mineral Density ◽

Bone Mineral ◽

Basic Protein ◽

Mineral Density ◽

Ghrelin Secretion ◽

Milk Basic Protein

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Role and advantage of cystatin C in kidney function monitoring during retroviral therapy

Diagnostyka Laboratoryjna ◽

10.5604/01.3001.0013.7961 ◽

2017 ◽

Vol 53 (1) ◽

pp. 33-40

Author(s):

Maria Warwas ◽

Katarzyna Juszczyńska ◽

Ewa Mulewicz ◽

Jakub Gburek

Keyword(s):

Kidney Function ◽

Cystatin C ◽

Clinical Investigation ◽

Inhibitory Effect ◽

Antiretroviral Drugs ◽

Hiv Protease ◽

Hiv Virus ◽

Infection And Inflammation ◽

Virus Strains

The aim of the study is to evaluate the utility of cystatin C (Cys C) determination in monitoring of HIV seropositive patients, based on recent literature concerning clinical investigation. Determination of serum CysC concentration can be helpful in monitoring the kidney function and eGFR (estimated GFR) calculation, however infection and inflammation markers influence should be included. A risk assessment of the appearance of cardiovascular incidents and risks of the all-cause mortality can be the other application for this parameter. The urinary CysC concentration can serve as the diagnostic marker of kidney tubular injuries triggered with adverse effects of antiretroviral drugs eg. tenofovir. In order to introduce applications into the routine clinical practice, further research is essential. Research concerning antiviral activity of cystatin C suggest, that CysC suppresses the viral replication due to inhibition of HIV protease, but in some cases its inhibitory effect on cathepsin B may be harmful and cause progression of the infection. In order that CysC could effectively use in the future, further experiments are needed to evaluate its effect on all sort virus strains, both dependent and independent of CD4+ T-lymphocytes, strains of the HIV virus.

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