STUDYING THE CONTENT OF PHENOLIC COMPOUNDS OF BUPLEURUM SCORZONERIFOLIUM WILLD., GROWING IN THE BAIKAL REGION WITH HPLC

In folk medicine in the Baikal region, an infusion from the aerial part of the plant Bupleurum scorzonerifolium (Willd) is widely used as a choleretic and anti-inflammatory agent. The choleretic effect of herbal remedies is mainly due to the content of phenylpropanoids and flavonoids. Using high performance liquid chromatography with a diode-matrix detector and 3Q electrospray ionization detector, we studied the metabolic profile of the phenylpropanoids and flavonoids of the aboveground organs of B. scorzonerifolium (Willd.), which grows in the Baikal region. In the aboveground organs of B. scorzonerifolium, 7 phenylpropanoids and 17 flavonoids were identified, of which 15 compounds for the species were identified for the first time. Quantitative determination of individual components was carried out in the aboveground organs of B. scorzonerifolium and dry extract by microcolumn HPLC with UV detection. The predominant components in the aerial part of the plant are 5-O-caffeic quinic acid, 3,5-di-O-caffeic quinic acid, isoquercitrin, isorhamnetin-3-O-glucoside, kaempferol-3-O-rhamnoside and rutin. The dry extract obtained from the aerial part of B. scorzonerifolium contains the entire complex of phenylpropanoids and flavonoids, which are identified in the plant. The content of active ingredients in the extract is 2.5–3.0 times higher than in the aboveground organs. The aboveground organs and dry extract of B. scorzonerifolium can be used as a source of phenylpropanoids and flavonoids.

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DYNAMIC CHANGES IN THE COMPOSITION OF BIOLOGICALLY ACTIVE COMPOUNDS OF BUPLEURUM SCORZONERIFOLIUM WILLD. AERIAL PART IN DIFFERENT PHENOLOGICAL PHASES

chemistry of plant raw material ◽

10.14258/jcprm.2020026323 ◽

2020 ◽

pp. 111-118

Author(s):

Zhargal Aleksandrovich Tykheev ◽

Vasiliy Vladimirovich Taraskin ◽

Svetlana Vasilyevna Zhigzhitzhapova ◽

Daba Gombocyrenovich Chimitov ◽

Larisa Dorzhievna Radnaeva

Keyword(s):

Phenolic Compounds ◽

Essential Oils ◽

Aerial Part ◽

Lipid Fraction ◽

Total Content ◽

Biologically Active ◽

Flowering Phase ◽

Bupleurum Scorzonerifolium Willd ◽

Bupleurum Scorzonerifolium ◽

Phenological Phases

The article is devoted to investigate the changes in the composition of biologically active substances (essential oils, lipid fraction, phenolic compounds) of the aerial part of the Bupleurum scorzonerifolium Willd. in depending on the phase of plant development. The greatest variety of compounds included in the composition of essential oils is characterized by a flowering phase. Fifteen terpene compounds – p-cymol, β-myrcene, trans-β-ocymene, limonene, (E,E)-α-farnesene, α-copaene, β-elemene, humulene, germacrene D, caryophyllene, γ-muurolene, bicyclogermacrene, Δ-cadinene, t-muurolol, spatulenol – are constant components of essential oils, regardless of phenophase. The quantitative content of these components varies from trace to major at different phenological phases. The coincidence of the main components of the lipid fraction was established. The main saturated acid is 16:0. Its content changes depending on the phase of vegetation – it is approximately at the same level determined in the phases of vegetation and flowering phases (26.78% and 27.26%, respectively) and there is a decrease in the fruit phase (18.17%). The main monounsaturated fatty acid is 18:1n9. Its greatest content was found in the fruiting phase, the smallest – flowering. The predominant polyunsaturated fatty acid is 18:2n9,12. Its greatest content was found in the vegetation phase (26.78%), the lowest - flowering (24.23%). However, the total content of unsaturated fatty acids exceeds the total content of saturated fatty acids in all phases of plant development. Although, their ratio varies from phenophase. There is a general pattern of highest content of phenolic compounds in the flowering phase for all studied samples. It was shown that during the flowering phase, the maximum accumulation of essential oils, lipids and phenolic compounds is observed.

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In Vitro Anti-Inflammatory and Immunomodulatory Activities of an Extract from the Roots of Bupleurum rotundifolium

Medicines ◽

10.3390/medicines6040101 ◽

2019 ◽

Vol 6 (4) ◽

pp. 101 ◽

Cited By ~ 2

Author(s):

Cholet ◽

Decombat ◽

Vareille-Delarbre ◽

Gainche ◽

Berry ◽

...

Keyword(s):

Mononuclear Cells ◽

Polymorphonuclear Neutrophils ◽

Ros Production ◽

Peripheral Blood Mononuclear ◽

Aerial Parts ◽

Bupleurum Scorzonerifolium Willd ◽

Anti Inflammatory ◽

Inflammatory Profile ◽

Bupleurum Scorzonerifolium

Background: Some Bupleurum species, such as the Bupleurum chinense DC. or the Bupleurum scorzonerifolium Willd have been extensively studied (especially their roots) for the treatment of inflammation. In contrast, only compounds extracted from the aerial parts of Bupleurum rotundifolium have been studied and showed anti-inflammatory or antiproliferative activities. This study was conducted to investigate the antioxidant, anti-inflammatory, and immunomodulatory effects of Bupleurum rotundifolium roots. Methods: To tackle the various aspects of inflammation, we studied in vitro a methanolic extract from the roots of Bupleurum rotundifolium on peripheral blood mononuclear cells (PBMCs), polymorphonuclear neutrophils (PMNs), and the monocytic cells THP-1. Its antioxidant capacities and iron-chelating activity were assessed. The extract was tested on THP-1 differentiation, reactive oxygen species (ROS) production by leukocytes, neutrophils chemotaxis, cytokines, PGE2 production, and NF-κB activation in PBMCs. Results: The extract showed a decreased ROS production in stimulated cells. It increased PBMC chemokine secretion and up-regulated the differentiation of THP-1 monocytes into macrophage-like cells, indicating a potential interest of the extract in the resolution of acute inflammation. In addition, the analysis of cytokine production suggests that Bupleurum rotundifolium has immunomodulatory properties. Conclusions: Cytokines secretion, especially IL-1β and IL-12p70, provided us with a set of indicators suggesting that the extract might be able to drive the polarization of macrophages and lymphocytes toward a Th2 anti-inflammatory profile in excessive inflammation.

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