Genotoxic Effect of Methotrexate on Bone Marrow Chromosomes and DNA of Male Albino Mice (Mus Musculus)

2016 ◽  
Vol 64 ◽  
pp. 350-363 ◽  
Author(s):  
Nagla Zaky Ibrahim El-Alfy ◽  
Mahmoud Fathy Mahmoud ◽  
Sally Ramadan Gabr El-Ashry
Keyword(s):  
Bone Marrow ◽  
Mus Musculus ◽  
Genotoxic Effect ◽  
Albino Mice

scholarly journals Genotoxic Effect of Silk Dyeing Wastes in Bone Marrow Cells of Mice, Mus musculus

CYTOLOGIA ◽  
2005 ◽  
Vol 70 (4) ◽  
pp. 381-384 ◽  
Author(s):  
Om Prakash Chaurasia ◽  
Alok Kumar ◽  
Moushmi Kumari
Keyword(s):  
Bone Marrow ◽  
Mus Musculus ◽  
Bone Marrow Cells ◽  
Genotoxic Effect ◽  
Silk Dyeing ◽  
Marrow Cells

scholarly journals Genotoxic effect of restraint and stress pheromone on somatic and germ cells of mouse males Mus musculus L.

2021 ◽  
Vol 19 (2) ◽  
pp. 169-179
Author(s):  
Veronika D. Shcherbinina ◽  
Marina V. Petrova ◽  
Timofey S. Glinin ◽  
Eugene V. Daev
Keyword(s):  
Bone Marrow ◽  
Comet Assay ◽  
Germ Cells ◽  
Mus Musculus ◽  
Genotoxic Effect ◽  
Genome Integrity ◽  
Cell Frequency ◽  
Testicular Cells ◽  
First Time ◽  
In Cells

BACKGROUND: Different stressors affect the genome integrity, but the mechanisms of such action are underexplored. MATERIALS AND METHODS: Bone marrow and testicular cells of CBA and CD-1 mouse males were used to estimate their genome integrity after stressor action by the comet assay. RESULTS: It is shown here that restraint and 2,5-dimethylpyrazine both increase damaged cell frequency in bone marrow as well as in testes of mouse males. For the first time the effect of immobilization and 2,5-dimethylpyrazine in testicular cells is demonstrated using the comet assay. Both stressors have similar effects in cells of both tissues analyzed. CONCLUSION: Mechanisms of the effects and possible role in microevolution are under discussion.

Investigation of the genotoxic effect in bone marrow of Swiss albino mice exposed long-term to pyrimethamine

2002 ◽  
Vol 22 (6) ◽  
pp. 393-402 ◽  
Author(s):  
B. Tunca ◽  
U. Egeli ◽  
N. Aydemir ◽  
G. Cecener ◽  
R. Bilaloglu
Keyword(s):  
Bone Marrow ◽  
Genotoxic Effect ◽  
Swiss Albino Mice ◽  
Albino Mice

scholarly journals Effect of the aqueous extract of Banana Fruits Peal Musa paradisiaca on Mitosis in Plant and Mammalian cells

2010 ◽  
Vol 7 (2) ◽  
pp. 858-866
Author(s):  
Baghdad Science Journal
Keyword(s):  
Bone Marrow ◽  
Allium Cepa ◽  
Aqueous Extract ◽  
Mus Musculus ◽  
Mammalian Cells ◽  
Test System ◽  
Root Tips ◽  
Significant Difference ◽  
Albino Mice

The aqueous extract of banana fruits peal was tested for its effect on mitosis . The root tips of Allium cepa were used as plant test system and the bone marrow cells of the albino mice Mus musculus were used as mammalians test system in vivo .Root tips of Allium cepa were treated for four hours with five concentrations of the extract (5 , 10 , 20 , 40 ,60 mg / ml.).The Metaphase was arrested in all the treatments , the highest percentage ( 100 % ) was recorded in the first concentration , the last concentration caused stickiness and clumping of the chromosomes. The treatments did not cause significant difference in the mitotic index. The peals extract (5 mg /ml) was compared with the extracts of fruits bulb, leaves and roots of banana plant, it was found that the extract of fruits peal is the best considering the highest percentages of arrested Metaphase in the root tips cells. The albino mice Mus musculus were injected intraperitonial with the peals extract ( 0.01 , 0.02, 0.04, 0.06, 0.08 mg / gm body weight), the percentages of arrested Metaphase in the bone marrow of these animals were comparable to the recorded percentages when the animals were injected with colchicine ( 0.01 mg / gm b.w.) .This study revealed the antimitotic activity of the aqueous extract of banana fruits peal on both the plant and mammalian cells in vivo. Studies will be conducted to investigate the effect of the extract and its components on the proliferation of cancer cells in vitro and in vivo.

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