scholarly journals Time Dependent Erythrocyte Morphometric Changes of Prolonged Stored Blood and its Effect on Target Post-transfusion Haematocrit of Splenectomised Mongrel Dogs

2021 ◽  
Author(s):  
N.H. Okereke ◽  
R.I. Udegbunam ◽  
S.O. Udegbunam ◽  
T.H. Ezeobialu ◽  
K.E. Ezenwaka
Keyword(s):  
Morphometric Parameters ◽  
Time Dependent ◽  
Intraoperative Blood Loss ◽  
Blood Storage ◽  
Mean Values ◽  
Group I ◽  
Citrate Phosphate Dextrose ◽  
Healthy Dogs ◽  
Stored Blood ◽  
Citrate Phosphate

Background: Mean values of erythrocytic morphometric parameters of very old blood and its effect on the target post-transfusion haematocrit changes of splenectomised dogs was studied. Methods: Two hundred and fifty milliliters of blood each were drawn from healthy dogs (n=6) into citrate phosphate dextrose adenine-1 anti-coagulated blood bags, preserved for 35 days for the evaluation of erythrocyte morphometric and viability parameters. Thereafter, twenty adult male splenectomised dogs were randomly assigned into 5 groups (n=3). Post-splenectomy, 4, 14, 21 and 28 day old blood (DOB) were transfused to groups II-V while group I animals were not transfused. Intraoperative blood loss was determined during the surgery while post-transfusion, animals haematocrit were assayed and used to calculate the targeted haematocrit. Result: Findings revealed irreversible progressive time dependent morphometric changes by day 14 of blood storage. Hence, it is recommended that for transfusion purposes, 4 DOB should be the hallmark as it achieved the desired haematocrit and no morphometric changes were observed from it.

scholarly journals OBSERVATIONS ON THE BIOCHEMICAL EFFECTS OF TRANSFUSION OF CITRATE-PHOSPHATE-DEXTROSE STORED BLOOD IN MAN

1976 ◽  
Vol 86 (8) ◽  
pp. 1272-1279 ◽  
Author(s):  
Robert N. Miller ◽  
Robert Engelhardt ◽  
John A. Collins ◽  
Eduardo Slatopolsky ◽  
Jack H. Ladenson
Keyword(s):  
Biochemical Effects ◽  
Citrate Phosphate Dextrose ◽  
Stored Blood ◽  
Citrate Phosphate

scholarly journals Anticoagulants on yield of bone marrow-derived mononuclear cells harvested from dogs

2015 ◽  
Vol 45 (11) ◽  
pp. 2044-2049
Author(s):  
Harald Fernando Vicente de Brito ◽  
Tobias Fernandes-Filho ◽  
Fernanda de Lima Brandão ◽  
Patrícia Yukiko Montaño ◽  
Luciane Maria Laskoski ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Flow Cytometry ◽  
Stem Cell ◽  
Iliac Crest ◽  
Mononuclear Cells ◽  
Cd44 Expression ◽  
Citrate Phosphate Dextrose ◽  
Healthy Dogs ◽  
Citrate Phosphate ◽  
Paired T Test

Cell therapy with bone marrow-derived mononuclear cells is an alternative to therapy with mesenchymal stem cell cultures. The aim of the present research was the comparison of the yield of bone marrow-derived mononuclear cells harvested from dogs with two different anticoagulants. Bone marrow was harvested from the iliac crest of five healthy dogs aged between 15 and 30 months, and the effect of two anticoagulant solutions, CPDA-1 (citrate phosphate dextrose adenine-1) and heparin, on the isolation of mononuclear cells was compared. Mononuclear cells were isolated in a density gradient and stained for CD9 and CD44 for characterization by flow cytometry. Means were compared using Student's paired t-test. Samples harvested with CPDA-1 yielded an average of 5.16x106 (±1.76x106) to 20.20x106 (±1.55x106) mononuclear cells/mL, whereas the yield of samples harvested with heparin varied between 4.56x106 (±0.69x106) and 24.30x106 (±2.12x106) mononuclear cells mL-1. By flow cytometry, mean percentage of double-stained cells varied from 1.96% (±0.64%) to 5.01% (±0.73%) for CPDA-1 and from 2.23% (±0.70%) to 7.27% (±0.97%) for heparin. No significant statistical differences were observed on yield or CD9 and CD44 expression. Further studies are recommended to assess efficacy of CPDA on mononuclear cell isolation.

scholarly journals Spectrin oxidation correlates with membrane vesiculation in stored RBCs

Blood ◽  
1987 ◽  
Vol 69 (6) ◽  
pp. 1777-1781 ◽  
Author(s):  
GM Wagner ◽  
DT Chiu ◽  
JH Qju ◽  
RH Heath ◽  
BH Lubin
Keyword(s):  
Shelf Life ◽  
Strong Correlation ◽  
Exchange Chromatography ◽  
Blood Bank ◽  
Disulfide Exchange ◽  
Citrate Phosphate Dextrose ◽  
Membrane Vesiculation ◽  
Stored Blood ◽  
Citrate Phosphate

Abstract An increase in spectrin oxidation in a variety of erythrocytes displaying a tendency to vesiculate has been previously described. To explore this relationship in more detail, we have studied blood stored in citrate-phosphate-dextrose-adenine under blood bank conditions because, in this system, vesiculation occurs slowly. Vesiculation was quantitated by measuring acetylcholinesterase release, and the extent of spectrin oxidation was detected by using thiol-disulfide exchange chromatography. A strong correlation (r = .92) was found between the extent of spectrin oxidation and vesiculation when blood from five donors was analyzed at weekly intervals during storage. This strongly suggests that spectrin oxidation plays a role in the formation of spectrin-free vesicles, thereby limiting the shelf life of stored blood.

scholarly journals The storage of hard-packed red blood cells in citrate-phosphate- dextrose (CPD) and CPD-adenine (CPDA-1)

Blood ◽  
1979 ◽  
Vol 54 (1) ◽  
pp. 280-284 ◽  
Author(s):  
E Beutler ◽  
C West
Keyword(s):  
Shelf Life ◽  
Blood Cells ◽  
Red Cells ◽  
Normal Donor ◽  
Red Cell ◽  
Packed Red Blood Cells ◽  
Citrate Phosphate Dextrose ◽  
Storage Cells ◽  
Stored Blood ◽  
Citrate Phosphate

Abstract The preservation of red cells “hard packed” to a hematocrit of over 80% from blood collected in citrate-phosphate-dextrose (CPD) or CPD-adenine (CPDA-1) has been investigated. After 21 days of storage, cells that had been collected in CPD solution had consumed most or all of the available glucose and manifested markedly impaired viability after reinfusion into the normal donor. In contrast, red cells prepared from blood collected in CPDA-1, a medium containing supplementary adenine and an increased amount of glucose, maintained higher glucose and adenosine triphosphate levels and, in most instances, manifested satisfactory posttransfusion viability. We emphasize that in addition to providing longer shelf life of stored blood, CPDA-1 provides a better hard-packed red cell concentrate for transfusion at 21 days.

scholarly journals Stability of hematological parameters of canine blood samples stored with citrate phosphate dextrose adenine-1 anticoagulated plastic vacutainers

2019 ◽  
Vol 12 (3) ◽  
pp. 449-453 ◽  
Author(s):  
Okereke Henry Nnamdi ◽  
Udegbunam Rita Ijeoma ◽  
Nnaji Theophilus Okaforx
Keyword(s):  
Blood Cell ◽  
Hematological Parameters ◽  
Blood Banking ◽  
Citrate Phosphate Dextrose ◽  
Healthy Dogs ◽  
The Stability ◽  
Wbc Count ◽  
Differential White Blood Cell ◽  
Citrate Phosphate ◽  
Rbc Count

Aim: The effect of citrate phosphate dextrose adenine-1 on the hematological parameters of stored Nigerian indigenous dog's blood with plastic vacutainer was investigated. This was done in view of determining the viability and stability of the studied parameters for blood banking purpose. Till date, there is no literature on the stability of whole blood of Nigerian indigenous dogs for blood banking purposes. Materials and Methods: A total of six apparently adult healthy dogs were sampled, and their blood was stored at 4°C and analyzed for their packed cell volume (PCV), hemoglobin (Hgb) concentration, red blood cell (RBC) count, total and differential white blood cell (WBC) count, platelet count (PC), mean corpuscular values (mean corpuscular Hgb [MCH], MCH concentration, and mean corpuscular volume [MCV]), blood potency of hydrogen (pH), and erythrocyte sedimentation rate (ESR) over a period of 14 days. Results: Significant changes were observed in some of the studied parameters. Of the 14 days' study period, PCV, Hgb concentration, total WBC count, PC, and neutrophil count showed no significant (p≥0.05) difference until day 2 post-storage (PS), while RBC count, ESR, MCV, and lymphocyte count showed no significant (p≥0.05) variation up until day 3 PS. Blood pH showed no significant (p≥0.05) variation within 24-h PS but was significantly (p≤0.05) higher than obtained values on days 1, 3, 7, 10, and 14 PS. Conclusion: Based on our finding, we could suggest that hematological laboratories and hemotherapists could use canine blood stored in a plastic vacutainer for 2-3 days.

scholarly journals The storage of hard-packed red blood cells in citrate-phosphate- dextrose (CPD) and CPD-adenine (CPDA-1)

Blood ◽  
1979 ◽  
Vol 54 (1) ◽  
pp. 280-284
Author(s):  
E Beutler ◽  
C West
Keyword(s):  
Shelf Life ◽  
Blood Cells ◽  
Red Cells ◽  
Normal Donor ◽  
Red Cell ◽  
Packed Red Blood Cells ◽  
Citrate Phosphate Dextrose ◽  
Storage Cells ◽  
Stored Blood ◽  
Citrate Phosphate

The preservation of red cells “hard packed” to a hematocrit of over 80% from blood collected in citrate-phosphate-dextrose (CPD) or CPD-adenine (CPDA-1) has been investigated. After 21 days of storage, cells that had been collected in CPD solution had consumed most or all of the available glucose and manifested markedly impaired viability after reinfusion into the normal donor. In contrast, red cells prepared from blood collected in CPDA-1, a medium containing supplementary adenine and an increased amount of glucose, maintained higher glucose and adenosine triphosphate levels and, in most instances, manifested satisfactory posttransfusion viability. We emphasize that in addition to providing longer shelf life of stored blood, CPDA-1 provides a better hard-packed red cell concentrate for transfusion at 21 days.

scholarly journals Characteristics of resveratrol and serotonin on antioxidant capacity and susceptibility to oxidation of red blood cells in stored human blood in a time-dependent manner

2017 ◽  
Vol 46 (1) ◽  
pp. 272-283 ◽  
Author(s):  
Zübeyir Huyut ◽  
Mehmet Ramazan Şekeroğlu ◽  
Ragıp Balahoroğlu ◽  
Mehmet Tahir Huyut
Keyword(s):  
Antioxidant Capacity ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Time Dependent ◽  
Dependent Manner ◽  
Protective Effects ◽  
Activity Time ◽  
Citrate Phosphate Dextrose ◽  
Citrate Phosphate ◽  
Over Time

Objective In stored red blood cells (RBCs), which are used in diseases (e.g., acute blood loss and leukaemia), storage lesions arise by oxidative stress and other factors over time. This study investigated the protective effects of resveratrol and serotonin on stored RBCs. Methods Blood from each donor (n = 10) was placed in different bags containing 70 mL of citrate phosphate dextrose (total volume: 500 mL) and divided into three groups (n = 30): control, 60 µg/mL resveratrol, and 60 µg/mL serotonin. Malondialdehyde (MDA) and glutathione (GSH) levels, activity of glutathione peroxidase (GSH-Px), catalase, and carbonic anhydrase (CA), and susceptibility to oxidation in RBCs, and pH in whole blood were measured at baseline and on days 7, 14, 21, and 28. Results MDA levels and susceptibility to oxidation were increased in all three groups time-dependently, but this increase was greater in the serotonin group than in the other groups. Activity of GSH-Px, CAT, and CA, as well as GSH levels, were decreased in the control and serotonin groups time-dependently, but were significantly preserved in the resveratrol group. The pH was decreased in all groups time-dependently. Conclusion Our study shows that resveratrol attenuates susceptibility to oxidation of RBCs and protects their antioxidant capacity, and partially preserves CA activity time-dependently.

scholarly journals Spectrin oxidation correlates with membrane vesiculation in stored RBCs

Blood ◽  
1987 ◽  
Vol 69 (6) ◽  
pp. 1777-1781
Author(s):  
GM Wagner ◽  
DT Chiu ◽  
JH Qju ◽  
RH Heath ◽  
BH Lubin
Keyword(s):  
Shelf Life ◽  
Strong Correlation ◽  
Exchange Chromatography ◽  
Blood Bank ◽  
Disulfide Exchange ◽  
Citrate Phosphate Dextrose ◽  
Membrane Vesiculation ◽  
Stored Blood ◽  
Citrate Phosphate

An increase in spectrin oxidation in a variety of erythrocytes displaying a tendency to vesiculate has been previously described. To explore this relationship in more detail, we have studied blood stored in citrate-phosphate-dextrose-adenine under blood bank conditions because, in this system, vesiculation occurs slowly. Vesiculation was quantitated by measuring acetylcholinesterase release, and the extent of spectrin oxidation was detected by using thiol-disulfide exchange chromatography. A strong correlation (r = .92) was found between the extent of spectrin oxidation and vesiculation when blood from five donors was analyzed at weekly intervals during storage. This strongly suggests that spectrin oxidation plays a role in the formation of spectrin-free vesicles, thereby limiting the shelf life of stored blood.

scholarly journals Assessment of Donkey (Equus asinus africanus) Whole Blood Stored in CPDA-1 and CPD/SAG-M Blood Bags

Biology ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 133
Author(s):  
Isabella Oliveira Barros ◽  
Rejane Santos Sousa ◽  
Marcondes Dias Tavares ◽  
Renato Otaviano Rêgo ◽  
Paulo Ricardo Firmino ◽  
...  
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
Whole Blood ◽  
Blood Collection ◽  
Blood Gas ◽  
Blood Cell Count ◽  
Animal Blood ◽  
Equus Asinus ◽  
Citrate Phosphate Dextrose ◽  
Citrate Phosphate

Hemotherapy using whole blood and its components is being increasingly used in veterinary therapy. Since it is important to store animal blood while maintaining acceptable hematological, blood gas, and biochemical characteristics, increasing our knowledge of available technologies for strategic blood storage is imperative. Thus, we aimed to assess the hematological, blood gas, and biochemical changes in donkey whole blood using blood bags with two different types of storage agents. Eight adult healthy male donkeys were used; 900 mL of blood was collected from each, with 450 mL stored in citrate-phosphate-dextrose and adenine bags (CPDA-1) and 450 mL stored in bags containing citrate-phosphate-dextrose, adenine, mannitol, and sodium chloride (CPD/SAG-M). Both bags were kept refrigerated between 1 and 6 °C for 42 days. Blood samples were removed from the bags eight times (T): T0 (immediately after blood collection), T1, T3, T7, T14, T21, T35, and T42 (1, 3, 7, 14, 21, 35 and 42 days after storage). Hematological, blood gas, biochemical, and microbiological parameters were assessed. The CPDA-1 bags had a higher packed cell volume when compared to CPD/ SAG-M. The red blood cell count reduced by around 19% in both the bags due to hemolysis, which was confirmed by an increase in plasma hemoglobin. The white blood cell count; pH; concentrations of glucose, sodium, bicarbonate, and 2,3 diphosphoglycerate were reduced in both bags. Meanwhile, pO2, pCO2, lactate dehydrogenase, and levels of potassium increased in the CPDA-1 and CPD/SAG-M bags. Blood bags were efficient for the storage of donkey blood for up to 42 days.

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