scholarly journals Serum Progesterone and Estradiol-17.BETA. Concentrations, and Lapaloscopic Observations of the Ovary in the Cheetah (Acinonyx jubatus) with Pregnant Mare Serum Gonadotropin and Human Chorionic Gonadotropin Treatments.

2001 ◽  
Vol 63 (12) ◽  
pp. 1361-1364 ◽  
Author(s):  
Osamu DOI ◽  
Hiroshi KUSUNOKI ◽  
Tetsuya SATO ◽  
Shigehisa KAWAKAMI ◽  
Toshio FUKUOKA ◽  
...  
2020 ◽  
Vol 129 (1A) ◽  
pp. 21-29
Author(s):  
Nguyễn Thi Thu Hiền ◽  
Nguyễn Thị Phương Thảo ◽  
Nguyễn Thanh Bình

Nghiên cứu này được thực hiện để đánh giá hiệu quả của kích dục tố (PMSG – pregnant mare serum gonadotropin, HCG – human chorionic gonadotropin) lên thời gian xuất hiện, thời gian kéo dài động dục và hiệu suất sinh sản trên đối tượng cầy vòi hương chậm động dục. Thí nghiệm được tiến hành trên 54 cá thể cầy vòi hương cái và 42 cá thể cầy đực tại trung tâm Công nghệ sinh học tỉnh Đồng Nai và trang trại động vật hoang dã Thanh Long. Chế phẩm PMSG/HCG (Gestavet –Vương quốc Anh) được tiêm bắp theo 3 công thức CT1: 20 IU PMSG + 10 IU HCG; CT2: 30 IU PMSG + 15 IU HCG; CT3: 40 IU PMSG + 20 IU HCG. Kết quả cho thấy thời gian xuất hiện động dục trung bình từ 1,1 ngày đến 2,6 ngày sau khi tiêm kích dục tố. CT3 có tác động động dục sớm nhất. Thời gian động dục kéo dài từ 2,9 đến 3,9 ngày; thời gian động dục ngắn nhất ở CT1 và dài nhất ở CT3 (p < 0,05). Tỉ lệ cầy động dục và mang thai tăng so với đối chứng ở tất cả các nhóm cầy thí nghiệm. Số con sinh ra trung bình từ 1,67 đến 3,25 con/lứa và cao nhất ở CT3 (p < 0,05). Khối lượng con sơ sinh từ 94,02 đến 97,25 g/cá thể. Kết quả cho thấy có thể áp dụng công thức CT3 có hiệu quả cho việc điều trị sự chậm lên giống lần đầu ở cầy tơ và trì hoãn động dục lại ở cầy cái sau khi sinh con.


2015 ◽  
Vol 13 (2) ◽  
pp. 189
Author(s):  
Agus Oman Sudrajat ◽  
Antharest Sugati ◽  
, Alimuddin

<p class="BasicParagraph" align="center"><strong>ABSTRACT</strong></p><p class="BasicParagraph" align="center"><strong> </strong></p><p class="Pa2">Artificial reproduction of eel <em>Anguilla bicolor </em>is not yet well-established because of insufficient broodstock number. In this research, induction of Indonesian eel gonad maturation was performed by hormonal with a combination of <em>pregnant mare serum gonadotropin </em>(PMSG), <em>human chorionic gonadotropin </em>(HCG) antidopamin and recombinant <em>growth hormone </em>(rGH). This research consisted of five treatments namely: control (NaCl 0,9%), PMSG 20 IU/ kg, PMSG 20 IU/kg + antidopamin 10 ppm/kg, PMSG 20 IU/kg + antidopamin 10 ppm/kg + rGH 10 μg/kg dan PMSG 20 IU/kg + HCG 10 IU/kg. Each treatment contained 10 fishes. Hormonal induction was conducted by intramuscular injections, as much as five times at intervals of seven days. Furthermore observations on gonadal development were performed after injection for 21 days. The results showed that the treatment generated pregnancy level of 100%, while control was 0%. The best treatment was PMSG 20 IU/kg + antidopamin 10 ppm/kg+ rGH 10 μg/kg, seen from a more mature phase of the gametes, spermatocytes in male and oocytes with perinukleolar phase in female fish. Eel at the body weight of 120.4 to 207.8 g and at the body length of 40.9 to 43.1 cm was male, at the body weight of 274.8 g and at the body length of 47 cm was in intersexual phase, and at the body weight of 323.4 g and at the body length of 53 cm was female.</p><p class="Default"> </p><p class="Pa2">Keywords: <em>Anguilla bicolor</em>, antidopamin, hormones, PMSG, rGH, HCG</p><br /><p class="BasicParagraph"> </p><p class="BasicParagraph"> </p><p class="BasicParagraph" align="center"><strong>ABSTRAK</strong></p><p class="BasicParagraph"><strong> </strong></p><p class="Pa2">Pemijahan ikan sidat secara buatan belum dapat dilakukan karena keterbatasan induk matang gonad. Penelitian ini dilakukan untuk mengetahui pengaruh pemberian hormon terhadap percepatan proses perkembangan gonad ikan sidat (<em>Anguilla bicolor</em>). Hormon yang digunakan adalah kombinasi dari <em>pregnant mare serum gonadotropin </em>(PMSG), <em>human chorionic gonadotropin </em>(HCG), antidopamin dan recombinant <em>growth hormone </em>(rGH). Induksi hormonal untuk mempercepat perkembangan gonad ikan sidat dilakukan melalui lima perlakuan yaitu yaitu kontrol (NaCl 0,9%), PMSG 20 IU/kg, PMSG 20 IU/kg+antidopamin 100 ppm/kg, PMSG 20 IU/kg+antidopamin 100 ppm/ kg+rGH 10 μg/kg dan PMSG 20 IU/kg+HCG 10 IU/kg. Setiap perlakuan dilakukan pada sepuluh ekor ikan sidat. Aplikasi induksi hormonal dilakukan melalui penyuntikan secara intramuskular sebanyak lima kali dengan interval tujuh hari sekali, selanjutnya dilakukan pengamatan terhadap perkembangan gonad selama 21 hari dengan interval tujuh hari sekali setelah penyuntikan. Hasil penelitian menunjukkan bahwa perlakuan hormonal menyebabkan tingkat kebuntingan sebanyak 100% pada ikan perlakuan, sedangkan kontrol sebanyak 0%. Kombinasi terbaik adalah PMSG+antidopamin+rGH, terlihat dari fase gamet yang lebih matang yaitu mencapai fase spermatosit pada ikan jantan dan oosit dengan fase perinukleolar pada ikan betina. Berdasarkan hasil penelitian, ikan sidat dengan bobot 120,4−207,8 g dan panjang 40,9−43,1 cm masih berjenis kelamin jantan. Ikan dengan bobot 274,8 g dan panjang 47 cm masih berada pada fase peralihan kelamin, sedangkan pada bobot 323,4 dan panjang 53 cm sudah berjenis kelamin betina.</p><p class="Default"> </p><p class="Pa2">Kata kunci: <em>Anguilla bicolor</em>, antidopamin, hormon, PMSG, rGH, HCG</p>


1979 ◽  
Vol 180 (3) ◽  
pp. 573-578 ◽  
Author(s):  
R R Dighe ◽  
K Muralidhar ◽  
N R Moudgal

Ability of the beta-subunit of human chorionic gonadotropin to inhibit the response to lutropin (luteinizing hormone, LH) was tested in the immature rat ovarian system and pregnant-mare-serum-gonadotropin-primed rat ovarian system with progesterone production being used as the response. Human chorionic gonadotropin beta-subunit was found to inhibit human and ovine lutropin-stimulated progesterone production. At a constant dose of lutropin, inhibition was dependent on the concentration of beta-subunit. When concentration of the beta-subunit was kept constant at 5.0 microgram/ml and the concentration of lutropin was varied, the inhibition was maximum at the saturating concentration of the native hormone. The alpha-subunit of the human chorionic gonadotropin did not inhibit the response to lutropin. The lutropin/beta-subunit ratio required to produce an inhibition of response was much lower than that required to bring about an observable inhibition of binding.


2017 ◽  
Vol 19 (2) ◽  
pp. 75
Author(s):  
Wiwin Kusuma Atmaja Putra ◽  
Rian Hadrianto ◽  
Tengku Said Razai

Human Chorionic Gonadotropin (hCG) and Pregnant Mare Serum Gonadotropin (PMSG) hormone is a product that contains the hormone Folicle Stimulating Hormone (FSH) and Litiunizing hormone (LH) that play a role in regulating the development gonad process of fish. This research determine to effect hCG and PMSG hormone induction and find out the best treatment in the induction on gonad maturation of Silver pompano fish. This research used three treatments: control (NaCl), hormone hCG 20 IU.kg-1 and PMSG 20 IU.kg-1 fish body weight. Containers used in the maintenance of fish in the form of Floating Net Cage (KJA) with size 3x3x3 m. The best research result is 20 IU PMSG treatment with GSI value equal to 0.6%, HSI equal to 1.25%, maturity level of gonad reaches TKG III and histologically progress reach secondary oocyte and primary oocyte. The conclusion of this research is hCG and PMSG hormone induction effect on gonad maturation Silver pompano fish for 4 weeks compared to control treatment. The best hormone treatment is PMSG hormone treatment with a dose of 20 IU. Kg-1 fish body weight.


1995 ◽  
Vol 41 (6) ◽  
pp. j89-j95 ◽  
Author(s):  
Yukari TSUDA ◽  
Shigeru NAKAO ◽  
Toshihiko NAKAO ◽  
Masaharu MORIYOSHI ◽  
Keiichiro KAWATA

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