scholarly journals Induced maturation of eel Anguilla bicolor using different hormone combination

2015 ◽  
Vol 13 (2) ◽  
pp. 189
Author(s):  
Agus Oman Sudrajat ◽  
Antharest Sugati ◽  
, Alimuddin
Keyword(s):  
Growth Hormone ◽  
Body Weight ◽  
Body Length ◽  
Chorionic Gonadotropin ◽  
Human Chorionic Gonadotropin ◽  
Gonadal Development ◽  
The Body ◽  
Gonad Maturation ◽  
Pregnant Mare Serum Gonadotropin ◽  
Serum Gonadotropin

<p class="BasicParagraph" align="center"><strong>ABSTRACT</strong></p><p class="BasicParagraph" align="center"><strong> </strong></p><p class="Pa2">Artificial reproduction of eel <em>Anguilla bicolor </em>is not yet well-established because of insufficient broodstock number. In this research, induction of Indonesian eel gonad maturation was performed by hormonal with a combination of <em>pregnant mare serum gonadotropin </em>(PMSG), <em>human chorionic gonadotropin </em>(HCG) antidopamin and recombinant <em>growth hormone </em>(rGH). This research consisted of five treatments namely: control (NaCl 0,9%), PMSG 20 IU/ kg, PMSG 20 IU/kg + antidopamin 10 ppm/kg, PMSG 20 IU/kg + antidopamin 10 ppm/kg + rGH 10 μg/kg dan PMSG 20 IU/kg + HCG 10 IU/kg. Each treatment contained 10 fishes. Hormonal induction was conducted by intramuscular injections, as much as five times at intervals of seven days. Furthermore observations on gonadal development were performed after injection for 21 days. The results showed that the treatment generated pregnancy level of 100%, while control was 0%. The best treatment was PMSG 20 IU/kg + antidopamin 10 ppm/kg+ rGH 10 μg/kg, seen from a more mature phase of the gametes, spermatocytes in male and oocytes with perinukleolar phase in female fish. Eel at the body weight of 120.4 to 207.8 g and at the body length of 40.9 to 43.1 cm was male, at the body weight of 274.8 g and at the body length of 47 cm was in intersexual phase, and at the body weight of 323.4 g and at the body length of 53 cm was female.</p><p class="Default"> </p><p class="Pa2">Keywords: <em>Anguilla bicolor</em>, antidopamin, hormones, PMSG, rGH, HCG</p><br /><p class="BasicParagraph"> </p><p class="BasicParagraph"> </p><p class="BasicParagraph" align="center"><strong>ABSTRAK</strong></p><p class="BasicParagraph"><strong> </strong></p><p class="Pa2">Pemijahan ikan sidat secara buatan belum dapat dilakukan karena keterbatasan induk matang gonad. Penelitian ini dilakukan untuk mengetahui pengaruh pemberian hormon terhadap percepatan proses perkembangan gonad ikan sidat (<em>Anguilla bicolor</em>). Hormon yang digunakan adalah kombinasi dari <em>pregnant mare serum gonadotropin </em>(PMSG), <em>human chorionic gonadotropin </em>(HCG), antidopamin dan recombinant <em>growth hormone </em>(rGH). Induksi hormonal untuk mempercepat perkembangan gonad ikan sidat dilakukan melalui lima perlakuan yaitu yaitu kontrol (NaCl 0,9%), PMSG 20 IU/kg, PMSG 20 IU/kg+antidopamin 100 ppm/kg, PMSG 20 IU/kg+antidopamin 100 ppm/ kg+rGH 10 μg/kg dan PMSG 20 IU/kg+HCG 10 IU/kg. Setiap perlakuan dilakukan pada sepuluh ekor ikan sidat. Aplikasi induksi hormonal dilakukan melalui penyuntikan secara intramuskular sebanyak lima kali dengan interval tujuh hari sekali, selanjutnya dilakukan pengamatan terhadap perkembangan gonad selama 21 hari dengan interval tujuh hari sekali setelah penyuntikan. Hasil penelitian menunjukkan bahwa perlakuan hormonal menyebabkan tingkat kebuntingan sebanyak 100% pada ikan perlakuan, sedangkan kontrol sebanyak 0%. Kombinasi terbaik adalah PMSG+antidopamin+rGH, terlihat dari fase gamet yang lebih matang yaitu mencapai fase spermatosit pada ikan jantan dan oosit dengan fase perinukleolar pada ikan betina. Berdasarkan hasil penelitian, ikan sidat dengan bobot 120,4−207,8 g dan panjang 40,9−43,1 cm masih berjenis kelamin jantan. Ikan dengan bobot 274,8 g dan panjang 47 cm masih berada pada fase peralihan kelamin, sedangkan pada bobot 323,4 dan panjang 53 cm sudah berjenis kelamin betina.</p><p class="Default"> </p><p class="Pa2">Kata kunci: <em>Anguilla bicolor</em>, antidopamin, hormon, PMSG, rGH, HCG</p>

scholarly journals Maturasi Gonad Bawal Bintang (Trachinotus blochii) dengan Induksi Hormon Human Chorionic Gonadotropin (hCG) dan Pregnant Mare Serum Gonadotropin (PMSG)

2017 ◽  
Vol 19 (2) ◽  
pp. 75
Author(s):  
Wiwin Kusuma Atmaja Putra ◽  
Rian Hadrianto ◽  
Tengku Said Razai
Keyword(s):  
Body Weight ◽  
Chorionic Gonadotropin ◽  
Human Chorionic Gonadotropin ◽  
Research Result ◽  
Hormone Treatment ◽  
Control Treatment ◽  
Gonad Maturation ◽  
Induction Effect ◽  
Pregnant Mare Serum Gonadotropin ◽  
Serum Gonadotropin

Human Chorionic Gonadotropin (hCG) and Pregnant Mare Serum Gonadotropin (PMSG) hormone is a product that contains the hormone Folicle Stimulating Hormone (FSH) and Litiunizing hormone (LH) that play a role in regulating the development gonad process of fish. This research determine to effect hCG and PMSG hormone induction and find out the best treatment in the induction on gonad maturation of Silver pompano fish. This research used three treatments: control (NaCl), hormone hCG 20 IU.kg-1 and PMSG 20 IU.kg-1 fish body weight. Containers used in the maintenance of fish in the form of Floating Net Cage (KJA) with size 3x3x3 m. The best research result is 20 IU PMSG treatment with GSI value equal to 0.6%, HSI equal to 1.25%, maturity level of gonad reaches TKG III and histologically progress reach secondary oocyte and primary oocyte. The conclusion of this research is hCG and PMSG hormone induction effect on gonad maturation Silver pompano fish for 4 weeks compared to control treatment. The best hormone treatment is PMSG hormone treatment with a dose of 20 IU. Kg-1 fish body weight.

scholarly journals PRESTASI BERANAK TERNAK BABI YANG MENGGUNAKAN HORMON PMSG DAN hCG PADA PETERNAKAN KOMERSIAL DI KELURAHAN KAYAWU

ZOOTEC ◽  
2013 ◽  
Vol 33 (1) ◽  
pp. 58
Author(s):  
Tery Wenda ◽  
Frans A. Kairupan ◽  
Petrus R. R. I. Montong ◽  
Sjaloom E. Sakul ◽  
Mien Th. R. Lapian
Keyword(s):  
Birth Weight ◽  
Litter Size ◽  
Chorionic Gonadotropin ◽  
Human Chorionic Gonadotropin ◽  
Follicle Stimulating Hormone ◽  
The Body ◽  
Average Birth Weight ◽  
Randomized Design ◽  
Serum Gonadotropin ◽  
Stimulating Hormone

ABSTRAKSuperovulasi merupakan salah satu upaya untuk meningkatkan derajat ovulasi dengan penyuntikan hormon gonadotropin pada tubuh hewan betina. Pada umumnya hewan donor disuntik dengan preparat follicle stimulating hormone (FSH) danPregnant mare's serum gonadotropin(PMSG) atau kombinasi (Pregnant mare's serum gonadotropin) dan human chorionic gonadotropin (hCG).Tujuan Penelitian iniadalah untuk mengetahui sejauh mana prestasi beranak babi dara (calon induk) dengan menggunakan hormon pregnant mare's serum gonadropin (PMSG) dan human Chorionic Gonadotropin (hCG).Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) terdiri atas dua perlakuan (menggunakan dan tanpa menggunakan hormon PMSG dan hCG) dengan masing-masing 21 ulangan. Semua data dianalisa dengan analisis sidik ragam (ANOVA) Steel dan Torrie (1993).Variabel yang diamati untuk adalah litter size, berat lahir anak babi yang dilahirkan dan mortalitas anak babi yang lahir sampai disapih. Hasil penelitian menunjukkan bahwa jumlah anak babi yang lahir hidup dalam satu kelahiran ada perbedaan sangat nyata bahwa yang lahir dari induk Superovulasi dibandingkan dengan induk yang tidak disuperovulasikan, rata-rata 9.19-6.66 ekor. Dan juga berat lahir rata-rata 1.53-1.40 kg Demikian juga mortalitas anak rata-rata 14.36-29.19%. Hasil penelitian menunjukkan bahwa penggunakan hormon PMSG dan hCG dapat memperbaiki litter size lahir, bobot lahir, dan menekan persentase mortalitas ternak babi. Kata Kunci:Potensi beranak babi dara,hormon PMSG dan hCG, Babi betina bunting.ABSTRACTTHE ACHIEVEMENT PIGS BEAR LIVESTOCK USING PMSG AND hCG HORMONE ON RANCH IN THE VILLAGE COMMERCIAL KAYAWU. Superovulation is an effort to increase the degree of ovulation by injection of gonadotropin hormones in the body of a female animal. In general, animal donors were injected with preparations of follicle stimulating hormone (FSH) and Pregnant mare's serum gonadotropin (PMSG) or a combination (Pregnant mare's serum gonadotropin) and human chorionic gonadotropin (hCG). The objective of this research was to determine the extent of achievement farrow dara (prospective parent) using pregnant mare's serum hormone gonadropin (PMSG) and human chorionic gonadotropin (hCG). The research using completely randomized design (CRD) consisting of two treatments (using and without using PMSG and hCG hormone) with 21 replications each. All data were analyzed with analysis of variance (ANOVA) Steel and Torrie (1993). Variables was observed for litter size, birth weight piglets born and piglets born mortality until weaning. The results showed that the number of piglets born alive in the birth there is a very real difference that is born of a parent superovulation compared with the parent who does not to superovulated on average 9.19–6.66 tail. And also the average birth weight 1:53 to 1:40 kilograms likewise the average child mortality 14.36–29.19%. From the results of the study concluded that the use of PMSG and hCG hormone can improve birth litter size, birth weight and reduce the percentage of mortality of pigs. Keywords: Extent of achievement of gilt,PMSG dan hCG hormone, and Pregnant mare.

scholarly journals ẢNH HƯỞNG CỦA KÍCH DỤC TỐ LÊN ĐỘNG THÁI SINH DỤC VÀ KHẢ NĂNG SINH SẢN CỦA CẦY VÒI HƯƠNG (Paradoxurus hermaphroditus) CHẬM ĐỘNG DỤC

2020 ◽  
Vol 129 (1A) ◽  
pp. 21-29
Author(s):  
Nguyễn Thi Thu Hiền ◽  
Nguyễn Thị Phương Thảo ◽  
Nguyễn Thanh Bình
Keyword(s):  
Chorionic Gonadotropin ◽  
Human Chorionic Gonadotropin ◽  
Pregnant Mare Serum Gonadotropin ◽  
Serum Gonadotropin

Nghiên cứu này được thực hiện để đánh giá hiệu quả của kích dục tố (PMSG – pregnant mare serum gonadotropin, HCG – human chorionic gonadotropin) lên thời gian xuất hiện, thời gian kéo dài động dục và hiệu suất sinh sản trên đối tượng cầy vòi hương chậm động dục. Thí nghiệm được tiến hành trên 54 cá thể cầy vòi hương cái và 42 cá thể cầy đực tại trung tâm Công nghệ sinh học tỉnh Đồng Nai và trang trại động vật hoang dã Thanh Long. Chế phẩm PMSG/HCG (Gestavet –Vương quốc Anh) được tiêm bắp theo 3 công thức CT1: 20 IU PMSG + 10 IU HCG; CT2: 30 IU PMSG + 15 IU HCG; CT3: 40 IU PMSG + 20 IU HCG. Kết quả cho thấy thời gian xuất hiện động dục trung bình từ 1,1 ngày đến 2,6 ngày sau khi tiêm kích dục tố. CT3 có tác động động dục sớm nhất. Thời gian động dục kéo dài từ 2,9 đến 3,9 ngày; thời gian động dục ngắn nhất ở CT1 và dài nhất ở CT3 (p < 0,05). Tỉ lệ cầy động dục và mang thai tăng so với đối chứng ở tất cả các nhóm cầy thí nghiệm. Số con sinh ra trung bình từ 1,67 đến 3,25 con/lứa và cao nhất ở CT3 (p < 0,05). Khối lượng con sơ sinh từ 94,02 đến 97,25 g/cá thể. Kết quả cho thấy có thể áp dụng công thức CT3 có hiệu quả cho việc điều trị sự chậm lên giống lần đầu ở cầy tơ và trì hoãn động dục lại ở cầy cái sau khi sinh con.

scholarly journals Effect of exogenous human chorionic gonadotropin on ovulation in mice

2020 ◽  
Vol 23 (8) ◽  
pp. 1006-1010
Author(s):  
S. Ya. Amstislavsky ◽  
S. V. Ranneva ◽  
D. S. Ragaeva ◽  
E. A. Chuyko ◽  
A. M. Popkova ◽  
...  
Keyword(s):  
Body Weight ◽  
Chorionic Gonadotropin ◽  
Human Chorionic Gonadotropin ◽  
Assisted Reproductive Technologies ◽  
Polar Body ◽  
Reproductive Technologies ◽  
The Body ◽  
Hormonal Stimulation ◽  
Experimental Group

The implementation of assisted reproductive technologies (ART), hormonal stimulation in particular, may change the quality of ovulated oocytes. The purpose of our work was to study ovulation in CD1 mice after their stimulation with human chorionic gonadotropin (hCG) and to investigate the effects of such hormonal stimulation on the pregnancy duration, fetal losses and the weight of the offspring. No significant differences were found in the total number of ovulated oocytes or in the number of immature (without a polar body) ovulated oocytes; nor were there differences between the groups in the number of oocytes with a developing polar body. However, the number of matured oocytes with a distinct polar body was significantly higher (p < 0.05) in mice stimulated with hCG (experimental group) as compared with the controls (6.2 ± 0.86 and 2.2 ± 0.97, respectively). No significant differences were observed between the experimental and control mice in the duration of pregnancy or in the numbers of term offspring, including the percentage of live and stillborn pups. However, the body weight of the offspring in the experimental group was significantly lower (p < 0.001) as compared with the controls on the fifth day after birth (3.16 ± 0.09 and 3.76 ± 0.07, respectively). Thus, exogenous hCG facilitates the development of mouse oocytes in vivo, which leads to the larger number of their mature forms at ovulation, however, the offspring born after hCG-stimulated pregnancy was characterized by a lower body weight on the fifth day after birth.

scholarly journals Ability of human chorionic gonadotropin β-subunit to inhibit the steroidogenic response to lutropin

1979 ◽  
Vol 180 (3) ◽  
pp. 573-578 ◽  
Author(s):  
R R Dighe ◽  
K Muralidhar ◽  
N R Moudgal
Keyword(s):  
Chorionic Gonadotropin ◽  
Human Chorionic Gonadotropin ◽  
Beta Subunit ◽  
Alpha Subunit ◽  
Immature Rat ◽  
Progesterone Production ◽  
Pregnant Mare Serum Gonadotropin ◽  
Serum Gonadotropin ◽  
Constant Dose ◽  
Human Chorionic Gonadotropin Beta

Ability of the beta-subunit of human chorionic gonadotropin to inhibit the response to lutropin (luteinizing hormone, LH) was tested in the immature rat ovarian system and pregnant-mare-serum-gonadotropin-primed rat ovarian system with progesterone production being used as the response. Human chorionic gonadotropin beta-subunit was found to inhibit human and ovine lutropin-stimulated progesterone production. At a constant dose of lutropin, inhibition was dependent on the concentration of beta-subunit. When concentration of the beta-subunit was kept constant at 5.0 microgram/ml and the concentration of lutropin was varied, the inhibition was maximum at the saturating concentration of the native hormone. The alpha-subunit of the human chorionic gonadotropin did not inhibit the response to lutropin. The lutropin/beta-subunit ratio required to produce an inhibition of response was much lower than that required to bring about an observable inhibition of binding.

scholarly journals GONAD REMATURATION ON Pangasionodon hypophthalmus FEMALE THROUGH INJECTION OF PREGNANT MARE SERUM GONADOTROPIN AND HUMAN CHORIONIC GONADOTROPIN

2014 ◽  
Vol 9 (1) ◽  
pp. 9
Author(s):  
Evi Tahapari ◽  
Jadmiko Darmawan ◽  
Raden Roro Sri Pudji Sinarni Dewi ◽  
Wahyu Pamungkas ◽  
Huria Marnis
Keyword(s):  
Chorionic Gonadotropin ◽  
Gonad Development ◽  
Gonad Maturation ◽  
Pangasianodon Hypophthalmus ◽  
Seed Availability ◽  
Pregnant Mare Serum Gonadotropin ◽  
Hormone Injection ◽  
Serum Gonadotropin ◽  
Internal And External Factors

The success of spawning is influenced by internal and external factors. One of the factors that affect the var iabi li ty of Pangasianodon hypophthalmus female reproductive is the change of seasons that cause disrupted continuity of the seed availability, especially in the dry season. In the present study, combination of PMSG (pregnant mare serum gonadotropin) + HCG (hormone chorionic gonadotropin) hormone injections was done to induce gonad development. The treatments in this study were without hormone injections as control (A), injection of PMSG 10 IU/kg + HCG 10 IU/kg (B), and injection of PMSG 20 IU/kg HCG + 10 IU/kg (C). Injections were conducted at intervals of two weeks as many as six times. The results showed that gonad maturation generally occurs 2-4 weeks after estradiol-17 peak. PMSG + HCG hormone injections gave a significant effect on increasing the quantity and quality of eggs production. The fecundity in the A, B, C treatments, were 233,700±220,676; 300,305±24,581 and 488,433±142,228; respectively. Number of larvae produced from the A, B, C treatments, were 156,979±170,838; 229,997±18,081 and 362,713±101,850; respectively. Combination of PMSG 20 IU/kg + HCG 10 IU/kg hormone injection gave the best result on fecundity and the number of larvae production.

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