In vitro Cultivation and Partial Characterization of Lawsonia intracellularis from a Japanese Field Case of Porcine Proliferative Enteropathy

AbstractProliferative enteropathy (PE; ileitis) is a common intestinal disease affecting susceptible pigs raised under various management systems around the world. Major developments in the understanding of PE and its causative agent,Lawsonia intracellularis, have occurred that have led to advances in the detection of this disease and methods to control and prevent it. Diagnostic tools that have improved overall detection and early onset of PE in pigs include various serological and molecular-based assays. Histological tests such as immunohistochemistry continue to be the gold standard in confirmingLawsonia-specific lesions in pigspost mortem. Despite extreme difficulties in isolatingL. intracellularis, innovations in the cultivation and the development of pure culture challenge models, have opened doors to better characterization of the pathogenesis of PE throughin vivoandin vitro L. intracellularis–host interactions. Advancements in molecular research such as the genetic sequencing of the entireLawsoniagenome have provided ways to identify various immunogens, metabolic pathways and methods for understanding the epidemiology of this organism. The determinations of immunological responsiveness in pigs to virulent and attenuated isolates ofL. intracellularisand identification of various immunogens have led to progress in vaccine development.

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In vitro conversion of proapoprotein A-I to apoprotein A-I. Partial characterization of an extracellular enzyme activity.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(17)44242-6 ◽

1983 ◽

Vol 258 (19) ◽

pp. 11430-11433 ◽

Cited By ~ 18

Author(s):

C Edelstein ◽

J I Gordon ◽

K Toscas ◽

H F Sims ◽

A W Strauss ◽

...

Keyword(s):

Enzyme Activity ◽

Extracellular Enzyme ◽

Extracellular Enzyme Activity ◽

Partial Characterization

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In Vitro Production of Angiotropic Factor by Bovine Corpus Luteum: Partial Characterization of Activities that are Chemotactic and Mitogenic for Endometrial Cells

Advances in Experimental Medicine and Biology - Regulation of Ovarian and Testicular Function ◽

10.1007/978-1-4684-5395-9_42 ◽

1987 ◽

pp. 683-688 ◽

Cited By ~ 8

Author(s):

D. A. Redmer ◽

J. D. Kirsch ◽

A. T. Grazul

Keyword(s):

Corpus Luteum ◽

Partial Characterization ◽

In Vitro Production ◽

Endometrial Cells ◽

Bovine Corpus Luteum ◽

Vitro Production

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In Vitro Cultivation and Morphological Characterization of Phloemic Trypanosomatids Isolated from Coconut Trees

Journal of Eukaryotic Microbiology ◽

10.1111/j.1550-7408.2009.00454.x ◽

2010 ◽

Vol 57 (1) ◽

pp. 87-93 ◽

Cited By ~ 1

Author(s):

DARLÃ G. KELLER ◽

FLÃVIO C. MIGUENS

Keyword(s):

Morphological Characterization ◽

In Vitro Cultivation

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In vitro Rearrangement and Deletion of Plasmid DNA Sequences: The Isolation and Partial Characterization of a Novel F-Controlled Plasmid Cloning Vehicle

Plasmids ◽

10.1007/978-3-642-81064-0_49 ◽

1977 ◽

pp. 407-415

Author(s):

J. J. Manis ◽

B. C. Kline

Keyword(s):

Dna Sequences ◽

Plasmid Dna ◽

Partial Characterization

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Cloning and Molecular Characterization of a Multicopy, Linear Plasmid-Carried, Repeat Motif-Containing Gene from Borrelia turicatae, a Causative Agent of Relapsing Fever

Journal of Bacteriology ◽

10.1128/jb.180.18.4974-4981.1998 ◽

1998 ◽

Vol 180 (18) ◽

pp. 4974-4981 ◽

Cited By ~ 15

Author(s):

Jason A. Carlyon ◽

Richard T. Marconi

Keyword(s):

Transcriptional Start Site ◽

Gene Families ◽

In Vitro Cultivation ◽

Relapsing Fever ◽

Related Sequence ◽

Site Analysis ◽

Low Levels ◽

Ribosomal Binding Site

ABSTRACT Borrelia turicatae is one of several spirochete species that can cause relapsing fever. Here, we describe the identification and characterization of a gene from B. turicatae and other relapsing-fever spirochetes that exhibits homology with therep + and ORF-E gene families of the Lyme disease spirochetes. This gene, which we have designatedrepA, encodes a putative protein of 30.2 kDa with an isoelectric point of 4.69. The central region of RepA harbors a series of amino acid repeat motifs which exhibit homology with casein kinase 2 phosphorylation sites. Through Southern hybridization analyses, we demonstrate that repA (or a closely related sequence) is multicopy in the relapsing-fever spirochetes and is carried on variably sized linear plasmids in bothBorrelia parkeri and B. turicatae. Transcriptional analyses demonstrate that repA is expressed, albeit at low levels, during in vitro cultivation ofB. turicatae. Transcriptional start site analysis revealed that repA is preceded by a consensus ribosomal binding site and an appropriately spaced promoter element. The sequence conservation, unique features, and multicopy status of repAand its homologs suggest that RepA may play an important genus-wide role in the biology of the Borrelia.

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