Identification of Single Nucleotide Polymorphisms in Selenium-Glutathione Peroxidase (GPX1) Gene
2019 ◽
Vol 7
(3)
◽
pp. 819-827
Keyword(s):
Human genetic variation is quite common with single nucleotide polymorphisms (SNPs) accounting for the majority of the variants. In the present study, primers for amplification of the appropriate part of the human GPX1 gene by polymerase chain reaction (PCR) were designed. The aim of this study was to develop a restriction fragment length polymorphism (RFLP) assay to detect and characterize GPX1 polymorphism in the coding region and validate the assays by sequencing. This study demonstrated that the RFLP method, which can be rapid, is reliable and valid as a tool for identifying the different polymorphisms with a high degree of specificity and sensitivity.
2017 ◽
Vol 62
(No. 7)
◽
pp. 269-275
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2017 ◽
Vol 31
◽
pp. 5-11
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2001 ◽
Vol 15
(18)
◽
pp. 1752-1759
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Keyword(s):
2008 ◽
Vol 5
(1)
◽
pp. 81-86
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Keyword(s):
2005 ◽
Vol 112
(2)
◽
pp. 358-365
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