Preparation of Calcium Hydroxide Loaded PVA Hydrogels and Their Antibacterial Effect

Background. Use of herbal compounds as an intracanal medicament in the field of endodontics has become noteworthy, one of which is the Aloe vera compound whose antibacterial effect has already been proven in the planktonic form of Enterococcus faecalis. The purpose of this study is to evaluate the antibacterial effect of Aloe vera on E. faecalis biofilms at the 4th and 6th week of development. Materials and Methods. 130 single root canal teeth without anomalies and caries were used. They were divided into two groups of 65 teeth for four and six weeks of biofilm production. Five samples of each group were examined for confirmation of biofilm formation under an electron microscope. Study groups were investigated with an antimicrobial agent as an intracanal medicament including 20 samples treated with Aloe vera, calcium hydroxide, and phosphate-buffered saline, and biofilm and survival of pathogens were investigated. Dentin chip suspensions were used for colony-forming unit (CFU) counting to estimate remaining E. faecalis counts. Results. The CFU mean in the 4th week subgroup in Aloe vera, phosphate-buffered saline, and calcium hydroxide was 0, 69166.66 ± 31688.58, and 25000 ± 30822.07, and in the 6th week, it was 136.36 ± 323.33, 95000 ± 12247.44, and 27501.66 ± 36570.34, respectively, which showed a significant difference between the used materials ( p < 0.05 ). Conclusion. Aloe vera, in contrast to calcium hydroxide, eliminated 4th and 6th week biofilms and showed remarkable antibacterial properties against E. faecalis biofilm. These results support potency of Aloe vera to use as a natural antimicrobial material in the intracanal medicament.

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Antibacterial effect of triantibiotic mixture versus calcium hydroxide in combination with active agents against Enterococcus faecalis biofilm

Dental Materials Journal ◽

10.4012/dmj.2014-090 ◽

2014 ◽

Vol 33 (6) ◽

pp. 733-738 ◽

Cited By ~ 4

Author(s):

Ali SHOKRANEH ◽

Ali Reza FARHAD ◽

Nastaran FARHADI ◽

Masoud SAATCHI ◽

Seyed Mohsen HASHEMINIA

Keyword(s):

Calcium Hydroxide ◽

Enterococcus Faecalis ◽

Antibacterial Effect

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The in vitro Antibacterial Effect of Iodine-potassium Iodide and Calcium Hydroxide in Infected Dentinal Tubules in Different Time Intervals

The Journal of Contemporary Dental Practice ◽

10.5005/jcdp-10-2-59 ◽

2009 ◽

Vol 10 (2) ◽

pp. 59-66 ◽

Cited By ~ 6

Author(s):

Anda Kfir ◽

Shaul Lin ◽

Amir Laviv ◽

Galit Sela ◽

Zvi Fuss

Keyword(s):

Calcium Hydroxide ◽

Potassium Iodide ◽

Antibacterial Effect ◽

Positive Control ◽

Control Group ◽

Time Intervals ◽

Dentinal Tubules ◽

Viable Bacteria ◽

Root Dentin

Abstract Aim The aim of this study was to evaluate the antibacterial effect of iodine-potassium iodide (IKI) and calcium hydroxide (CH) on dentinal tubules infected with Enterococcus faecalis (E. faecalis) at different time intervals. Methods and Materials Hollow cylinders of bovine root dentin (n=45) were infected and divided into three equal groups filled with either IKI or CH and a positive control. After placing each medicament in the infected cylinders for time periods of 10 minutes, 48 hours and 7 days, microbiological samples were analyzed. At the end of each period, four 100 μm thick inner dentin layers (400 μm thick from each specimen) were removed using dental burs of increasing diameters. Dentin powder was cultured on agar plates to quantitatively assess their infection, expressed in colony forming units (cfu). Results In all layers of the positive control group, heavy bacterial infection was observed. After 10 minutes, IKI reduced the amount of viable bacteria more efficiently than CH, whereas at later time intervals CH showed the best results. Conclusion For short periods of exposure, IKI has a more efficient antibacterial effect in the dentinal tubules than CH but CH performs better after longer durations of exposure. Clinical Significance This research indicates the use of IKI is a better choice for disinfecting the root canal than CH if only a short duration of exposure is used because of its more efficient antibacterial effect. However, if a longer exposure time is used, then CH is a better choice because of its better disinfecting effect over time. Citation Lin S, Kfir A, Laviv A, Sela G, Fuss Z. The in vitro Antibacterial Effect of Iodine-potassium Iodide and Calcium Hydroxide in Infected Dentinal Tubules in Different Time Intervals. J Contemp Dent Pract 2009 March; (10)2:059-066.

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