The in vitro Antibacterial Effect of Iodine-potassium Iodide and Calcium Hydroxide in Infected Dentinal Tubules in Different Time Intervals

2009 ◽  
Vol 10 (2) ◽  
pp. 59-66 ◽  
Author(s):  
Anda Kfir ◽  
Shaul Lin ◽  
Amir Laviv ◽  
Galit Sela ◽  
Zvi Fuss
Keyword(s):  
Calcium Hydroxide ◽  
Potassium Iodide ◽  
Antibacterial Effect ◽  
Positive Control ◽  
Control Group ◽  
Time Intervals ◽  
Dentinal Tubules ◽  
Viable Bacteria ◽  
Root Dentin

Abstract Aim The aim of this study was to evaluate the antibacterial effect of iodine-potassium iodide (IKI) and calcium hydroxide (CH) on dentinal tubules infected with Enterococcus faecalis (E. faecalis) at different time intervals. Methods and Materials Hollow cylinders of bovine root dentin (n=45) were infected and divided into three equal groups filled with either IKI or CH and a positive control. After placing each medicament in the infected cylinders for time periods of 10 minutes, 48 hours and 7 days, microbiological samples were analyzed. At the end of each period, four 100 μm thick inner dentin layers (400 μm thick from each specimen) were removed using dental burs of increasing diameters. Dentin powder was cultured on agar plates to quantitatively assess their infection, expressed in colony forming units (cfu). Results In all layers of the positive control group, heavy bacterial infection was observed. After 10 minutes, IKI reduced the amount of viable bacteria more efficiently than CH, whereas at later time intervals CH showed the best results. Conclusion For short periods of exposure, IKI has a more efficient antibacterial effect in the dentinal tubules than CH but CH performs better after longer durations of exposure. Clinical Significance This research indicates the use of IKI is a better choice for disinfecting the root canal than CH if only a short duration of exposure is used because of its more efficient antibacterial effect. However, if a longer exposure time is used, then CH is a better choice because of its better disinfecting effect over time. Citation Lin S, Kfir A, Laviv A, Sela G, Fuss Z. The in vitro Antibacterial Effect of Iodine-potassium Iodide and Calcium Hydroxide in Infected Dentinal Tubules in Different Time Intervals. J Contemp Dent Pract 2009 March; (10)2:059-066.

scholarly journals Pengaruh Pemberian Ekstrak Teripang Pasir terhadap Hambatan Pertumbuhan Shigella Dysentriae Secara In Vitro

2020 ◽  
Vol 18 (1) ◽  
pp. 47
Author(s):  
FERIZAL NEGERI SAMUDRA ◽  
RETNO BUDIARTI ◽  
IRMAWATI IRMAWATI
Keyword(s):  
Sea Cucumber ◽  
Antibacterial Effect ◽  
Positive Control ◽  
Negative Control ◽  
Shigella Dysenteriae ◽  
Control Group ◽  
Control Groups ◽  
Shigella Spp ◽  
Diarrhea Disease

<p><strong>ABSTRACT</strong></p><p><strong>Background</strong>; In Indonesia, most diarrhea disease in 1995 to 2001 are caused by Shigella spp. Shigella spp infection can cause various symptom dan complication. Generally, the treatment by using antibiotic can cause antibiotic resistance. Sea cucumber (Holoturia scabra) is an herb that known, available, and easy to consume by society and has an antibacterial effect. Therefore, further research to study the effect of Holoturia Scabra on <em>Shigella Dysentriae</em> growth in vitro is needed.</p><p><strong>Objectives</strong>: The goal of this research is demonstrate the effect of sea cucumber (Holoturia scabra) to the growth of the <em>Shigella dysentriae</em> bacteria in vitro.</p><p><strong>Method</strong>: The method in this research is Posttest Only Control Group. There are 6 groups, 4 types of and 2 control groups. The concentration of the treatment group is 100%,50%, 25%, and, 12.5% while for positive control tests using chloramphenicol and aquadest as a negative control.</p><p><strong>Result</strong>: The result showed there is an influence on the intake of sand cucumber to the growth of the Shigella dysenteriae.</p><p><strong>Conclusion</strong>: Sea cucumber (<em>Holoturia scabra</em>) inhibit the growth of <em>Shigella dysenteriae</em>.</p><p><strong>Key words</strong>: <em>Shigella dysenteriae</em>, sea cucumber (<em>Holoturia scabra</em>), antibacterial</p>

scholarly journals Comparison of different irrigation and agitation methods for the removal of two types of calcium hydroxide medicaments from the root canal wall: An in-vitro study

2017 ◽  
Vol 90 (3) ◽  
pp. 327-332 ◽  
Author(s):  
Deepak Singh Kirar ◽  
Pradeep Jain ◽  
Pallav Patni
Keyword(s):  
Calcium Hydroxide ◽  
Root Canal ◽  
Positive Control ◽  
Positive Control Group ◽  
Control Group ◽  
Passive Ultrasonic Irrigation ◽  
Ultrasonic Irrigation ◽  
Group 2 ◽  
Group 1

Background and aim: Comparison of different irrigation and agitation methods for the removal of two types of calcium hydroxide medicaments from the root canal walls.Methods: Fifty extracted single rooted teeth were selected for this study. After decoronation, the root canals of these teeth were prepared to the size F3 (30 no.) using rotary ProTaper file system. These samples were randomly divided into four groups. Group 1 (n=20) were filled completely with water based calcium hydroxide (CH), Group 2 (n=20) were filled with oil based CH using lentulo spiral, Group 3 (n=5) - the positive control group received the CH as intracanal medication, but no subsequent removal, Group 4 (n=5) - the negative control did not receive CH placement. Further on, Group 1 and Group 2 were divided into four sub-groups (n=5). In sub-group A we performed conventional syringe irrigation with side-vented needle sub-group B) manual dynamic agitation, sub-group C sonic agitation using endoactivator, sub-group D passive ultrasonic irrigation (PUI). Roots were split longitudinally into mesial and distal halves. Digital images of the root canal walls were acquired by a Dental Operating Microscope (DOM) and assessed by using a scoring criteria at different thirds (coronal, middle and apical) of the root canal as follows: score 1, score 2, score 3, and score 4. Data were analyzed applying one-way analysis of variance (ANOVA) and Tukey’s multiple comparison tests at a 95% confidence interval (P < 0.05).Results: Statistically significant differences were not found between the experimental groups and the negative group in any one third of the root canal (P>0.05). However, a difference did exist between the experimental groups and the positive control group (P<0.05). None of the experimental groups totally removed CH substances from root canal walls.Conclusion: Among all experimental groups, removal of CH was best achieved by sonic agitation using endoactivator followed by passive ultrasonic irrigation (PUI), manual dynamic agitation and conventional syringe irrigation with side-vented needle.

scholarly journals Amylase-Producing Maltooligosaccharide Provides Potential Relief in Rats with Loperamide-Induced Constipation

2020 ◽  
Vol 2020 ◽  
pp. 1-9 ◽  
Author(s):  
Eun Yeong Jang ◽  
Yejin Ahn ◽  
Hyung Joo Suh ◽  
Ki-Bae Hong ◽  
Kyungae Jo
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Positive Control ◽  
Lactobacillus Bulgaricus ◽  
Control Group ◽  
Sprague Dawley ◽  
Potential Relief ◽  
Bowel Movements ◽  
Mucosal Thickness

Constipation is a chronic disease caused by infrequent, inadequate, and difficult bowel movements. The present study aimed to evaluate the potential laxative effect of maltooligosaccharide (MOS) on loperamide-induced constipation in a rat model. In vitro experiments were conducted to evaluate the effect of MOS on the growth of lactic acid bacteria. Moreover, to examine the effect of MOS administration on Sprague-Dawley (SD) rats with loperamide-induced constipation, the drinking water for the rats was supplemented with 10% or 15% of MOS for 14 days, and, thereafter, the improvement in constipation was assessed. For this, the rats were divided into five groups: normal (Nor), loperamide-induced constipated (Con), positive control (15% of dual-oligosaccharide (DuO-15)), 10% MOS treated (MOS-10), and 15% MOS-treated (MOS-15). In an in vitro test, MOS treatment promoted the growth of lactic acid bacteria except Lactobacillus bulgaricus. Treatment with higher MOS dose relieved constipation in rats by improving the fecal pellet and water content. Furthermore, in the high MOS dose group, the cecal short-chain fatty acid levels significantly increased compared to those in the control group (P<0.001). MOS treatment also improved the mucosal thickness as well as mucin secretion and increased the area of intestinal Cajal cells compared to that in the control group (P<0.001). These findings suggest that MOS relieves constipation and has beneficial effect on the gastrointestinal tract, and, therefore, it can be used as an ingredient in functional foods for treating constipation or improving intestinal health.

scholarly journals Development of Novel Fermented Almond Milk Tea and It’s Evaluation as Antidiabetic Drink

2021 ◽  
pp. 75-87
Author(s):  
Deepak Kannan ◽  
Akash Kumaran ◽  
Sanjay Venkatesan ◽  
Prabhu Sukumaran
Keyword(s):  
Diabetes Mellitus ◽  
Plasma Glucose ◽  
Sensory Analysis ◽  
Animal Studies ◽  
Positive Control ◽  
Positive Control Group ◽  
Control Group ◽  
Group I ◽  
Human Volunteers

Background: It is well known that almond and tea is best known to prevent Diabetes mellitus due to its abundant source of polyphenols. Also, probiotics also have been used in the treatment of Diabetes. This study is focused on the combined effect of all these three ingredients through the process of fermentation. Objective: The aim of this present study is to develop, analyse sensory parameters in human volunteers for optimisation and evaluate the antidiabetic efficiency of Fermented Almond milk tea (FAMT) both in vitro and in vivo analysis. Study Design: Development of FAMTàOptimisation of FAMT based on sensory analysis from 25 human participantsà In vitro antidiabetic analysis of FAMT extract à Animal studies. Place and Duration: The research work was conducted during November, 2019 to March, 2020 at the Department of Biotechnology, Sri Venkateswara College of Engineering, Post Bag No.1, Pennalur, Sriperumbudur Tk, Kancheepuram Dt, TN-602117, India. Materials and Methods: FAMT was prepared by optimisation of different formulation based on sensory analysis recorded from 25 healthy human volunteers. The FAMT extract was prepared and was used for the in vitro analysis and phytochemical screening. The animal study was performed with 30 Albino Wistar rats which were divided into 5 groups under preventive regimen. Group I was healthy normoglycemic control group. Group II served as positive control. Group III received metformin (350 mg/kg bw, p.o) for 28 days. Group IV received 5% Fermented almond milk for 28 days. Group V received 5% FAMT for 28thday. All groups except Group I received single dose of STZ (50 mg/kg bw, i.p) on the 29th day for the induction of Diabetes mellitus. After 7 days from induction, animals were anaesthetized and blood was drawn for the evaluation of plasma glucose and serum TG, cholesterol & insulin.   Results: It was observed that FAMT (8:2) was favoured by the participants more than other formulations. FAMT was found to contain Saponins, flavonoids and phenol. The total poly phenol of FAMT (373 ± 3.0 μg/ml) was high than Fermented almond milk (232.5 ± 2.50 μg/ml). The DPPH scavenging, α-amylase and α-glucosidase inhibiting percentage of FAMT (59 ± 4%,52 ± 3%, 50 ± 4% respectively) was high when compared to fermented almond milk (32 ± 2%,34 ± 2% and 45 ± 2% respectively). From animal studies it was significantly observed that plasma glucose (P<0.0001) was reduced, serum insulin (P<0.001) was increased, serum TG (P<0.0.001) and cholesterol (P<0.01) were reduced when compared to Positive control Group- II. Conclusion: Thus, FAMT was proved to act as a prophylactic anti-diabetic drink and was more potent than normal fermented almond milk.

scholarly journals Promotion of Dental Pulp Wound Healing in New Zealand White Rabbits’ Teeth by Thai Propolis Product

2018 ◽  
Vol 36 (1) ◽  
pp. 17-24 ◽  
Author(s):  
Nattriya Likitpongpipat ◽  
Somboon Sangmaneedet ◽  
Poramaporn Klanrit ◽  
Rajda Noisombut ◽  
Suttichai Krisanaprakornkit ◽  
...  
Keyword(s):  
Wound Healing ◽  
New Zealand ◽  
Calcium Hydroxide ◽  
Median Number ◽  
Positive Control ◽  
Negative Control ◽  
Histological Evaluation ◽  
Dentinal Tubules ◽  
Pulp Capping ◽  
New Zealand White Rabbits

This study examined and compared wound healing between Thai propolis product and calcium hydroxide paste as pulp-capping agents after partial pulpotomy in New Zealand white rabbits. Forty incisor teeth from 10 rabbits were treated. Thirty-six teeth received class V cavity preparations with partial pulpotomy and application of either propolis or calcium hydroxide paste. Similar cavity preparations were performed in 2 teeth without any capping material as a positive control, whereas 2 teeth without the cavity preparation served as a negative control. Histological evaluation showed that both groups had dentin bridge formation. Dentinal tubules in the dentin bridge were more orderly arranged in the Thai propolis group than in the calcium hydroxide group. Wound healing and the median number of hyperemic blood vessels were not statistically significant different between the 2 groups. Thai propolis product may be used as a pulp-capping agent.

Antibacterial Efficacy of Diode and Er:YAG Laser Irradiation in Experimentally Contaminated Primary Molar Root Canals

2009 ◽  
Vol 34 (1) ◽  
pp. 43-48 ◽  
Author(s):  
Senem Selvi Kuvvetli ◽  
Nuket Sandalli ◽  
Nursen Topcuoglu ◽  
Guven Kulekci
Keyword(s):  
Laser Irradiation ◽  
Diode Laser ◽  
Er:Yag Laser ◽  
Positive Control ◽  
Control Group ◽  
Antibacterial Efficacy ◽  
Primary Molar ◽  
Root Canals ◽  
Number Of Bacteria

Objective: In vitro comparison of the antibacterial efficacy of Diode and Er:YAG laser irradiation with that of NaOCl irrigation in contaminated primary molar root canals. Study Design: 96 root canals prepared from 32 extracted primary molar teeth were mechanically enlarged and the teeth were randomly divided into 4 subgroups. The roots were inoculated with an overnight culture of Enterococcus faecalis in tryptic soy broth for 24 hours. The root canals irradiated with diode and Er:YAG laser and irrigated with NaOCl(5.25%) were experimental groups and untreated canals served as positive control group. Bacterial growth was analysed by counting viable E.faecalis on tryptic soy agar plates. Results: The number of bacteria was significantly reduced in experimental groups in comparison with the control group. Diode laser was determined to be more effective in reducing the number of bacteria when compared to Er:YAG laser. NaOCl irrigation was found significantly most effective. Conclusions: Diode laser irradiation and 5.25 % NaOCl application provided a significant antibacterial effect in vitro, in contaminated primary molar root canals.

scholarly journals Effect of antibacterial photodynamic therapy on Streptococcus mutans plaque biofilm in vitro

2020 ◽  
Vol 13 (06) ◽  
pp. 2050022
Author(s):  
Xiaoyue Liang ◽  
Zhaohui Zou ◽  
Zheng Zou ◽  
Changyi Li ◽  
Xiaoxi Dong ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Lactic Acid ◽  
Streptococcus Mutans ◽  
Antibacterial Effect ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Saline Control ◽  
Control Group ◽  
Plaque Biofilm

The main objective of this study is to evaluate the antibacterial effect of antibacterial photodynamic therapy (aPDT) on Streptococcus mutans (S. mutans) biofilm model in vitro. The selection of photosensitizers is the key step for the efficacy of photodynamic therapy (PDT). However, no studies have been conducted in the oral field to compare the functional characteristics and application effects of PDT mediated by various photosensitizers. In this research, the antibacterial effect of Methylene blue (MB)/650[Formula: see text]nm laser and Hematoporphyrin monomethyl ether (HMME)/532[Formula: see text]nm laser on S. mutans biofilm was compared under different energy densities to provide experimental reference for the clinical application of the two PDT. The yield of lactic acid was analyzed by Colony forming unit (CFU) and spectrophotometry, and the complete biofilm activity was measured by Confocal Laser Scanning Microscopy (CLSM) to evaluate the bactericidal effect on each group. Based on the results of CFU, the bacterial colonies formed by 30.4[Formula: see text]J/cm2 532[Formula: see text]nm MB-aPDT group and 30.4[Formula: see text]J/cm2 532[Formula: see text]nm HMME-aPDT group were significantly less than those in other groups, and the bacterial colonies in HMME-aPDT group were less than those in HMME-aPDT group. Lactic acid production in all treatment groups except the photosensitizer group was statistically lower than that in the normal saline control group. The activity of bacterial plaque biofilm was significantly decreased in the two groups treated with 30.4[Formula: see text]J/cm2 aPDT. Therefore, aPDT suitable for energy measurement can kill S. mutans plaque biofilm, and MB-aPDT is better than HMME-aPDT.

scholarly journals IN VITRO EVALUATION OF THE ANTHELMINTIC ACTIVITY OF RHIZOME EXTRACTS OF CURCUMA LONGA (LINN.)

2018 ◽  
Vol 11 (12) ◽  
pp. 425
Author(s):  
Jyoti Pandey ◽  
Suman Mishra ◽  
Kamal Jaiswal
Keyword(s):  
Curcuma Longa ◽  
Anthelmintic Activity ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Phytochemical Analysis ◽  
Alternative Source ◽  
Significant Efficacy ◽  
Phosphate Buffered Saline

Objective: The current study was carried out to evaluate the anthelmintic activity of the rhizome extract of Curcuma longa as an alternative source of effective remedies for nematodiasis.Methods: The anthelmintic activity of the C. longa was assessed in vitro against Haemonchus spp., a gastrointestinal (abomasum) parasite of goats. Different concentrations of the extracts (1 mg/mL, 2.5 mg/mL, 5 mg/mL, and 10 mg/mL) in phosphate-buffered saline (PBS) were tested, and the results expressed in terms of time of paralysis (minute) and time of death (minute) of the worms. Albendazole (1 mg/mL) was used as a reference (positive control) and PBS as a control group (negative control).Results: The qualitative phytochemical analysis of the methanolic extract (ME) of the plant disclosed the presence of alkaloids, glycosides, terpenoids, flavonoids, tannins, saponins, phenol, anthraquinone, and carbohydrates; whereas, the aqueous extract (AE) showed the presence of alkaloids, carbohydrate, flavonoids, and saponins. Both ME and AE of the C. longa (rhizome) expressed significant efficacy (p≤0.05) in causing paralysis as well as the death of the worms within 12 h of exposure at all tested concentrations, as compared to the negative control. The rhizome extracts of C. longa showed dose-dependent efficacy in causing paralysis of the worm motility and the final progression to death. The results showed that the ME at 10 mg/mL was significantly more potent (p≤0.05) over the AE.Conclusions: This study concluded that the rhizome extract of C. longa exhibited potent anthelmintic efficacy against the nematode parasite, Haemonchus spp.

Sign in / Sign up

Export Citation Format

Share Document