1506 In vivo Three-Dimensional Mapping of Mesenteric Blood Vessels and Blood Flow Using GPU

2013 ◽  
Vol 2013.50 (0) ◽  
pp. 150601-150602
Author(s):  
Hikari ICHISHIMA ◽  
Yudai NAKAYAMA ◽  
Hiroki ISHIDA ◽  
Shunsuke AKIGUCHI ◽  
Tadashi HACHIGA ◽  
...  
Keyword(s):  
Blood Flow ◽  
Blood Vessels ◽  
Three Dimensional ◽  
Three Dimensional Mapping ◽  
Dimensional Mapping

Imaging System for Three-Dimensional Mapping of Cerebrocortical Capillary Networks in Vivo

1993 ◽  
Vol 46 (3) ◽  
pp. 293-309 ◽  
Author(s):  
Antal G. Hudetz ◽  
Andrew S. Greene ◽  
Gabriella Fehér ◽  
Derek E. Knuese ◽  
Allen W. Cowley
Keyword(s):  
Imaging System ◽  
Three Dimensional ◽  
Capillary Networks ◽  
Three Dimensional Mapping ◽  
Dimensional Mapping

scholarly journals Correlated magnetic resonance imaging and ultramicroscopy (MR-UM) is a tool kit to assess the dynamics of glioma angiogenesis

eLife ◽  
2016 ◽  
Vol 5 ◽  
Author(s):  
Michael O Breckwoldt ◽  
Julia Bode ◽  
Felix T Kurz ◽  
Angelika Hoffmann ◽  
Katharina Ochs ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Magnetic Resonance ◽  
Ex Vivo ◽  
Three Dimensional ◽  
Resonance Imaging ◽  
Tumor Microvasculature ◽  
Three Dimensional Mapping ◽  
Magnetic Resonance Imaging Mri ◽  
Dimensional Mapping

Neoangiogenesis is a pivotal therapeutic target in glioblastoma. Tumor monitoring requires imaging methods to assess treatment effects and disease progression. Until now mapping of the tumor vasculature has been difficult. We have developed a combined magnetic resonance and optical toolkit to study neoangiogenesis in glioma models. We use in vivo magnetic resonance imaging (MRI) and correlative ultramicroscopy (UM) of ex vivo cleared whole brains to track neovascularization. T2* imaging allows the identification of single vessels in glioma development and the quantification of neovessels over time. Pharmacological VEGF inhibition leads to partial vascular normalization with decreased vessel caliber, density, and permeability. To further resolve the tumor microvasculature, we performed correlated UM of fluorescently labeled microvessels in cleared brains. UM resolved typical features of neoangiogenesis and tumor cell invasion with a spatial resolution of ~5 µm. MR-UM can be used as a platform for three-dimensional mapping and high-resolution quantification of tumor angiogenesis.

scholarly journals High-speed volumetric two-photon fluorescence imaging of neurovascular dynamics

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Jiang Lan Fan ◽  
Jose A. Rivera ◽  
Wei Sun ◽  
John Peterson ◽  
Henry Haeberle ◽  
...  
Keyword(s):  
Blood Flow ◽  
High Speed ◽  
Laser Scanning ◽  
Three Dimensional ◽  
Laser Scanning Microscopy ◽  
Fluorescence Signal ◽  
Imaging Method ◽  
Spatiotemporal Resolution ◽  
Two Photon

AbstractUnderstanding the structure and function of vasculature in the brain requires us to monitor distributed hemodynamics at high spatial and temporal resolution in three-dimensional (3D) volumes in vivo. Currently, a volumetric vasculature imaging method with sub-capillary spatial resolution and blood flow-resolving speed is lacking. Here, using two-photon laser scanning microscopy (TPLSM) with an axially extended Bessel focus, we capture volumetric hemodynamics in the awake mouse brain at a spatiotemporal resolution sufficient for measuring capillary size and blood flow. With Bessel TPLSM, the fluorescence signal of a vessel becomes proportional to its size, which enables convenient intensity-based analysis of vessel dilation and constriction dynamics in large volumes. We observe entrainment of vasodilation and vasoconstriction with pupil diameter and measure 3D blood flow at 99 volumes/second. Demonstrating high-throughput monitoring of hemodynamics in the awake brain, we expect Bessel TPLSM to make broad impacts on neurovasculature research.

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