194 Determination of fluid mechanical effects caused by near wall blood flow field on endothelial cell damage : effect of shear stress on cell peeling of cultured endothelial cells

Purpose. To compare the changes in corneal endothelial cells after pars plana Ahmed glaucoma valve (AGV) implantation with those after the anterior chamber AGV implantation for refractory glaucoma.Methods. The medical records of 18 eyes with pars plana implantation of AGV (ppAGV) were reviewed retrospectively and were compared with 18 eyes with the anterior chamber AGV (acAGV) implant. The preoperative and postoperative endothelial cells, intraocular pressure (IOP), and postoperative complications during the follow-up in both groups were compared.Results. The average follow-up was 18 months. The postoperative endothelial cells in the ppAGV and acAGV groups were 2044 ± 303 and 1904 ± 324, respectively(P=0.25). The average percentage decrease in the endothelial cells in the ppAGV and acAGV groups at 18 months was 12.5% and 18.4%, respectively, and showed significant difference between the 2 groups(P=0.01). No difference in IOP control and the number of postoperative glaucoma medications was observed between the 2 groups.Conclusions. Endothelial cell damage in the ppAGV group for refractory glaucoma appeared to be lower than that in the acAGV group. Therefore, pars plana implantation of AGV may be preferred as it may have lower level of endothelial cell damage while maintaining similar level of IOP control.

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Inhibition of glutathione synthesis in brain endothelial cells lengthens S-phase transit time in the cell cycle: Implications for proliferation in recovery from oxidative stress and endothelial cell damage

Redox Biology ◽

10.1016/j.redox.2013.01.003 ◽

2013 ◽

Vol 1 (1) ◽

pp. 131-139 ◽

Cited By ~ 9

Author(s):

Carmina Buşu ◽

Wei Li ◽

Gloria Caldito ◽

Tak Yee Aw

Keyword(s):

Oxidative Stress ◽

Cell Cycle ◽

Endothelial Cells ◽

Endothelial Cell ◽

Transit Time ◽

Cell Damage ◽

S Phase ◽

Brain Endothelial Cells ◽

Endothelial Cell Damage ◽

Glutathione Synthesis

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Relationship between Candida albicans Virulence during Experimental Hematogenously Disseminated Infection and Endothelial Cell Damage In Vitro

Infection and Immunity ◽

10.1128/iai.72.1.598-601.2004 ◽

2004 ◽

Vol 72 (1) ◽

pp. 598-601 ◽

Cited By ~ 79

Author(s):

Angela A. Sanchez ◽

Douglas A. Johnston ◽

Carter Myers ◽

John E. Edwards ◽

Aaron P. Mitchell ◽

...

Keyword(s):

Endothelial Cells ◽

Candida Albicans ◽

Endothelial Cell ◽

Cell Damage ◽

Endothelial Cell Damage ◽

Disseminated Infection ◽

Lethal Infection ◽

Cell Lining ◽

Wild Type Parent

ABSTRACT Candida albicans must penetrate the endothelial cell lining of the vasculature to invade the deep tissues during a hematogenously disseminated infection. We compared 27 C. albicans mutants with their wild-type parent for their capacity to damage endothelial cells in vitro and cause a lethal infection in mice following tail vein inoculation. Of 10 mutants with significantly impaired capacity to damage endothelial cells, all had attenuated virulence. Therefore, the endothelial cell damage assay can be used as a screen to identify some virulence factors relevant to hematogenously disseminated candidiasis.

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Vitamin D ameliorated endothelial cell damage induced by diabetes via regulation of lncRNA MEG3 in human umbilical vein endothelial cells

Archives of Medical Science ◽

10.5114/aoms.2020.101248 ◽

2020 ◽

Author(s):

Hua Wei ◽

Qiongfang Zhang ◽

Jun Li ◽

Jing Yang ◽

Bin Huang ◽

...

Keyword(s):

Vitamin D ◽

Endothelial Cells ◽

Endothelial Cell ◽

Cell Damage ◽

Umbilical Vein ◽

Nuclear Volume ◽

Endothelial Cell Damage ◽

Tnf Α ◽

Gene And Protein Expression ◽

Umbilical Vein Endothelial Cells

IntroductionThe purpose of this study was to investigate long non-coding RNA (lncRNA) maternally expressed gene 3 (MEG3) effects in vitamin D (Vit. D) treatment in endothelial cell damage induced by diabetes.Material and methodsWe used human umbilical vein endothelial cells (HUVECs) as a research objective in our study and used high glucose in a diabetic cell model. We evaluated cell apoptosis by flow cytometry, inflammatory factors (IL-6, IL-1β and TNF-α) concentrations by ELISA assay, relative gene and protein expression by RT-qPCR and WB assay, and NF-κB(p65) nuclear volume by cellular immunofluorescence.ResultsCompared with the NC (normal control) group, the cell apoptosis rate was significantly increased, inflammatory factor (IL-6, IL-1β and TNF-α) concentrations were significantly up-regulated, lncRNA MEG3 gene expression was significantly depressed, Toll-like receptor 4 (TLR4), myeloid differentiation factor 88 (MyD88) and nuclear factor B p65 (NF-κB(p65)) gene and protein expression levels were significantly increased and NF-κB(p65) nuclear volume was significantly up-regulated (p < 0.001, respectively). With Vit. D supplementation, compared with the Model group, Vit. D improved endothelial cell damage induced by diabetes, while lncRNA MEG3 was significantly increased and the TLR4/MyD88/NF-κB(p65) pathway was significantly depressed dose-dependently (all p < 0.05). With sh-MEG3 transfection, the Vit. D treatment effects were significantly reduced.ConclusionsVit. D improved endothelial cell damage induced by diabetes via lncRNA MEG3 up-regulation in vitro study.

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