scholarly journals Study on Genome Sequence of Novel Corona virus (Sars-Cov-2) Strains in Different Countries

2020 ◽  
Vol 13 (4) ◽  
pp. 2015-2024
Author(s):  
Vishal Trivedi ◽  
Kush Biswas ◽  
Santosh Fattepur ◽  
Nagaraja Sreeharsha
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Southern China ◽  
Respiratory Illness ◽  
Molecular Diagnostic ◽  
Sequencing Analysis ◽  
Oxford Nanopore ◽  
Febrile Respiratory Illness ◽  
Novel Coronavirus

In Present time Novel coronavirus (SAR-CoV-2) was the biggest outbreak on human kind. SARS recognized febrile respiratory illness. It was first discovered in southern China in November 2002, and spread all other countries.Now SARS-CoV-2 was also originated from Wuhan, china. It was transmitted from human to human. For developing a molecular diagnostic for 2019-nCoV a PCR detection assay was developed. Sanger, Illumina, and Oxford nanopore techniques are used for sequencing analysis for 2019-nCoV.Based on different types of data base it was concluded that the SARS-CoV-2 was less mutated than other seasonal flu. This study shows the complete genome sequence and characteristic of SAR-CoV-2. SAR-CoV-2 has 29,903 nucleotides in length which is quite similar to others coronavirus. A complete genome sequence of different-different countries was studied.

scholarly journals Characterization and Complete Genome Sequence of a Novel Coronavirus, Coronavirus HKU1, from Patients with Pneumonia

2005 ◽  
Vol 79 (2) ◽  
pp. 884-895 ◽  
Author(s):  
Patrick C. Y. Woo ◽  
Susanna K. P. Lau ◽  
Chung-ming Chu ◽  
Kwok-hung Chan ◽  
Hoi-wah Tsoi ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Significant Proportion ◽  
Respiratory Illness ◽  
Immunoglobulin M ◽  
Additional Specimen ◽  
Group 2 ◽  
Novel Coronavirus ◽  
Tract Infections

ABSTRACT Despite extensive laboratory investigations in patients with respiratory tract infections, no microbiological cause can be identified in a significant proportion of patients. In the past 3 years, several novel respiratory viruses, including human metapneumovirus, severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV), and human coronavirus NL63, were discovered. Here we report the discovery of another novel coronavirus, coronavirus HKU1 (CoV-HKU1), from a 71-year-old man with pneumonia who had just returned from Shenzhen, China. Quantitative reverse transcription-PCR showed that the amount of CoV-HKU1 RNA was 8.5 to 9.6 × 106 copies per ml in his nasopharyngeal aspirates (NPAs) during the first week of the illness and dropped progressively to undetectable levels in subsequent weeks. He developed increasing serum levels of specific antibodies against the recombinant nucleocapsid protein of CoV-HKU1, with immunoglobulin M (IgM) titers of 1:20, 1:40, and 1:80 and IgG titers of <1:1,000, 1:2,000, and 1:8,000 in the first, second and fourth weeks of the illness, respectively. Isolation of the virus by using various cell lines, mixed neuron-glia culture, and intracerebral inoculation of suckling mice was unsuccessful. The complete genome sequence of CoV-HKU1 is a 29,926-nucleotide, polyadenylated RNA, with G+C content of 32%, the lowest among all known coronaviruses with available genome sequence. Phylogenetic analysis reveals that CoV-HKU1 is a new group 2 coronavirus. Screening of 400 NPAs, negative for SARS-CoV, from patients with respiratory illness during the SARS period identified the presence of CoV-HKU1 RNA in an additional specimen, with a viral load of 1.13 × 106 copies per ml, from a 35-year-old woman with pneumonia. Our data support the existence of a novel group 2 coronavirus associated with pneumonia in humans.

scholarly journals Complete Genome Sequence of a Coxsackievirus A16 Strain, Isolated from a Fatal Case in Shenzhen, Southern China, in 2014

2015 ◽  
Vol 3 (2) ◽  
Author(s):  
Long Chen ◽  
Hong Yang ◽  
Qian-Jin Feng ◽  
Xiang-Jie Yao ◽  
Hai-Long Zhang ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Southern China ◽  
Fatal Case ◽  
Coxsackievirus A16

scholarly journals Complete Genome Sequence of an ON1 Human Respiratory Syncytial Virus Strain Isolated in Lebanon in 2015

2018 ◽  
Vol 6 (16) ◽  
pp. e00316-18 ◽  
Author(s):  
Amani Ezzeddine ◽  
Nadia Soudani ◽  
Chun Kiat Lee ◽  
Ghassan Dbaibo ◽  
Husni Elbahesh ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
Emergency Room ◽  
Genome Sequence ◽  
Virus Strain ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Respiratory Illness ◽  
Human Respiratory Syncytial Virus ◽  
Acute Respiratory Illness ◽  
Syncytial Virus

ABSTRACT We report here the complete genome sequence of a human respiratory syncytial virus (HRSV) strain obtained from an infant who presented to the emergency room with an acute respiratory illness during the 2014/2015 HRSV season in Lebanon. Analysis revealed that this virus belongs to the ON1 genotype that has recently emerged worldwide.

scholarly journals Characterization of an Avian Influenza Virus H9N2 Strain Isolated from Dove in Southern China

2018 ◽  
Vol 6 (18) ◽  
pp. e00369-18 ◽  
Author(s):  
Dan Li ◽  
ZhengTing Li ◽  
Zhixun Xie ◽  
Meng Li ◽  
Zhiqin Xie ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Influenza Virus ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Wild Bird ◽  
Southern China

ABSTRACT We report here the complete genome sequence of strain H9N2, an avian influenza virus (AIV) isolated from dove in Guangxi, China. Phylogenetic analysis showed that it was a novel reassortant AIV derived from chicken, duck, and wild bird. This finding provides useful information for understanding the H9N2 subtype of AIV circulating in southern China.

scholarly journals Complete Genome Sequence of Rubrobacter xylanophilus Strain AA3-22, Isolated from Arima Onsen in Japan

2019 ◽  
Vol 8 (34) ◽  
Author(s):  
Natsuki Tomariguchi ◽  
Kentaro Miyazaki
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Hot Spring ◽  
Sequencing Data ◽  
Short Read ◽  
Content Type ◽  
Short Read Sequencing ◽  
Oxford Nanopore ◽  
Long Read

Rubrobacter xylanophilus strain AA3-22, belonging to the phylum Actinobacteria, was isolated from nonvolcanic Arima Onsen (hot spring) in Japan. Here, we report the complete genome sequence of this organism, which was obtained by combining Oxford Nanopore long-read and Illumina short-read sequencing data.

scholarly journals Complete Genome Sequence of a Rabies Virus Isolate from Cattle in Guangxi, Southern China

2013 ◽  
Vol 1 (1) ◽  
Author(s):  
H.-B. Tang ◽  
X.-X. He ◽  
Y.-Z. Zhong ◽  
S.-H. Liao ◽  
T.-Z. Zhong ◽  
...  
Keyword(s):  
Rabies Virus ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Virus Isolate ◽  
Southern China

scholarly journals Complete Genome Sequence of Feline Calicivirus Strain GX01-2013 Isolated from Household Cats in Guangxi, Southern China

2016 ◽  
Vol 4 (5) ◽  
Author(s):  
Ping He ◽  
Huabo Zhou ◽  
Weifeng Zhao ◽  
Jianqiao He ◽  
Ruikai Li ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Mdck Cells ◽  
Southern China ◽  
Feline Calicivirus ◽  
Genetic Characteristics

Here, we report the complete genome of a feline calicivirus (FCV) originating from household cats in Guangxi, southern China, in September 2013. To understand its genetic characteristics, we isolated FCV strain GX01-2013 from MDCK cells and determined its complete genome sequence.

scholarly journals Genome Sequencing of a Novel Coronavirus SARS-CoV-2 Isolate from Iraq

2021 ◽  
Vol 10 (4) ◽  
Author(s):  
Nihad A. M. Al-Rashedi ◽  
Danilo Licastro ◽  
Sreejith Rajasekharan ◽  
Simeone Dal Monego ◽  
Alessandro Marcello ◽  
...  
Keyword(s):  
Severe Acute Respiratory Syndrome ◽  
Genome Sequencing ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Illumina Miseq ◽  
Spike Protein ◽  
Protein Coding ◽  
Novel Coronavirus ◽  
First Time

ABSTRACT The coding-complete genome sequence of a severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) strain isolated from an Iraqi patient was sequenced for the first-time using Illumina MiSeq technology. There was a D614G mutation in the spike protein-coding sequence. This report is valuable for better understanding the spread of the virus in Iraq.

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