scholarly journals Comparison of ORAGENE® and Mouthwashed-Based Saliva Collection Methods for Genomic DNA Isolation

2015 ◽  
Vol 8 (2) ◽  
Author(s):  
Ayesha A. Hassan
Keyword(s):  
Genomic Dna ◽  
Dna Isolation ◽  
Cost Effective ◽  
Non Invasive ◽  
Genomic Dna Isolation ◽  
Invasive Method ◽  
Saliva Collection ◽  
The Difference ◽  
Collection Methods

In recent years, saliva has been used as a non-invasive method of obtaining genomic DNA. Two common collection methods include mouthwash and commercially produced saliva kits. Here, a novel comparison between these two collection methods, using Scope® mouthwash and the Oragene®-Discover kit (OGR-250) from DNA Genotek Inc., was conducted to analyze differences in the quantity and quality of the DNA isolated, and cost effectiveness. The Oragene® kit yielded greater quantity of DNA, while Scope® mouthwash was more cost effective. The difference in yield was attributed to the larger volume of saliva obtained from the Oragene® kit. Isolation from both collection methods resulted in similar DNA quality.    Depuis quelques années, la salive est utilisée comme une méthode non-invasive pour obtenir de l’ADN génomique. Deux méthodes de collection communes sont par rince-bouche et par des trousses commerciales de collection de salive. Ici, une comparaison entre ces deux méthodes, utilisant la rince-bouche Scope et la trousse Oragene-Discover (OGR-250) de DNA Genotek Ink, a été conduite afin d’analyser les différences dans la quantité et la qualité d’ADN isolée ainsi que dans l’efficacité du coût.  La trousse Oragene a recueilli plus d’ADN, alors que Scope était moins cher. La différence en quantité est attribuée au plus grand volume de salive qui est obtenu grâce à l’Oragene. L’isolation par les deux méthodes résultait en une qualité similaire d’ADN.

scholarly journals Using a Microwave Sensor as an Online Indicator of Neurological Impairment during Surgical Procedures

2013 ◽  
Vol 543 ◽  
pp. 368-372 ◽  
Author(s):  
Jung H. Goh ◽  
Alex Mason ◽  
Mark Field ◽  
Paul Browning ◽  
Ahmed Al-Shamma'a
Keyword(s):  
Cost Effective ◽  
Neurological Impairment ◽  
Treatment Regimes ◽  
Non Invasive ◽  
Microwave Sensor ◽  
Wide Range ◽  
Invasive Method ◽  
Aortic Aneurysm Surgery ◽  
Online Methods

Lactate is known to be an indicator of neurological impairment during aortic aneurysm surgery. It is suggested that analysis of cerebrospinal fluid (CSF) removed during such surgery could provide useful information in this regard. Medical professionals find the prospect of online detection of such analytes exciting, as current practice is time consuming and leads to multiple invasive procedures. Advancing from the current laboratory based analysis techniques to online methods could provide the basis for improved treatment regimes, better quality of care, and enhanced resource efficiency within hospitals. Accordingly, this article considers the use of a low power microwave sensor to detect varying lactate concentrations. Microwave sensors provide a rapid non-invasive method of material analysis, which is robust, cost-effective, and has huge potential for a wide range of biomedical applications.

scholarly journals Development of a Competent and Trouble Free DNA Isolation Protocol for Downstream Genetic Analyses in Glycine Species

2016 ◽  
Vol 4 (8) ◽  
pp. 700 ◽  
Author(s):  
Muhammad Amjad Nawaz ◽  
Faheem Shehzad Baloch ◽  
Hafiz Mamoon Rehman ◽  
Bao Le ◽  
Fahima Akther ◽  
...  
Keyword(s):  
Molecular Biology ◽  
Dna Extraction ◽  
Genomic Dna ◽  
Dna Isolation ◽  
Plant Molecular Biology ◽  
Pcr Amplification ◽  
Cost Effective ◽  
Extraction Methods ◽  
Glycine Species ◽  
Genomic Dna Isolation

Extraction of deoxyribose nucleic acid (DNA) from plants is preliminary step in molecular biology. Fast and cost effective genomic DNA isolation from Glycine species for downstream application is a major bottleneck. Here we report a high throughput and trouble free method for genomic DNA extraction from leaf and seeds of Glycine species with high quality and quantity. Protocol reports the optimization by employing different concentrations of CTAB and PVP in extraction buffer. Efficiency of optimized protocol was compared with frequently used DNA extraction methods. Wide adoptability and utility of this protocol was confirmed by DNA extraction from leaves as well as seeds of G. max, G. soja, G. tomentella and G. latifolia. Extracted DNA was successfully subjected to PCR amplification of five microsatellite markers and four putative glycosyltransferase genes. DNA extraction protocol is reproducible, trouble free, rapid and can be adopted for plant molecular biology applications.

scholarly journals Reconstruction of input functions from a dynamic PET image with sequential administration of 15O2 and H215O for noninvasive and ultra-rapid measurement of CBF, OEF, and CMRO2

2017 ◽  
Vol 38 (5) ◽  
pp. 780-792 ◽  
Author(s):  
Nobuyuki Kudomi ◽  
Yukito Maeda ◽  
Hiroyuki Yamamoto ◽  
Yuka Yamamoto ◽  
Tetsuhiro Hatakeyama ◽  
...  
Keyword(s):  
Rate Constants ◽  
Human Subjects ◽  
Invasive Procedure ◽  
Invasive Technique ◽  
Invasive Approach ◽  
Non Invasive ◽  
Sequential Administration ◽  
Invasive Method ◽  
The Difference ◽  
Arterial Input Functions

CBF, OEF, and CMRO2 images can be quantitatively assessed using PET. Their image calculation requires arterial input functions, which require invasive procedure. The aim of the present study was to develop a non-invasive approach with image-derived input functions (IDIFs) using an image from an ultra-rapid O2 and C15O2 protocol. Our technique consists of using a formula to express the input using tissue curve with rate constants. For multiple tissue curves, the rate constants were estimated so as to minimize the differences of the inputs using the multiple tissue curves. The estimated rates were used to express the inputs and the mean of the estimated inputs was used as an IDIF. The method was tested in human subjects ( n = 24). The estimated IDIFs were well-reproduced against the measured ones. The difference in the calculated CBF, OEF, and CMRO2 values by the two methods was small (<10%) against the invasive method, and the values showed tight correlations ( r = 0.97). The simulation showed errors associated with the assumed parameters were less than ∼10%. Our results demonstrate that IDIFs can be reconstructed from tissue curves, suggesting the possibility of using a non-invasive technique to assess CBF, OEF, and CMRO2.

scholarly journals Influence of hyperbaric oxygenation and its use in urinary tract diseases

2016 ◽  
Vol 56 (3) ◽  
pp. 25-32
Author(s):  
Małgorzata Skiba ◽  
Agnieszka Pedrycz
Keyword(s):  
Urinary Tract ◽  
Hyperbaric Oxygen ◽  
Hyperbaric Oxygen Therapy ◽  
Oxygen Therapy ◽  
Hemorrhagic Cystitis ◽  
Positive Influence ◽  
Non Invasive ◽  
Invasive Method ◽  
Hyperbaric Therapy

Abstract In this publication, we adduce examples of the use of hyperbaric oxygen therapy in urinary tract diseases. Hyperbaric oxygen therapy has been proved to have a positive influence on the kidneys of animals with diabetes, sepsis or undergoing chemotherapy. In the literature, we can also find many examples of the use of hyperbaric therapy with good clinical outcomes in human patients with prostatic hypertrophy, pyelonephritis, and hemorrhagic cystitis. The first trials of this kind of treatment of urinary tract diseases were started at the end of the twentieth century. In spite of the promising results, and numerous reports on the effectiveness of this non-invasive method of treatment, it is not currently used on a regular basis. Because many factors such as time, multiple applications, the parameters used in the hyperbaric chamber as well as the medications taken by the patient affect the quality of the result, further studies are needed to make hyperbaric therapy more suitable and safer for each patient.

Genomic DNA Isolation, Amplification of the D1S80 Locus and DNA-Gel Electrophoresis

2015 ◽  
pp. 42-42
Author(s):  
Arti Pandey ◽  
Arun Pandey ◽  
Naveen Shreevastava ◽  
Durga Neupane
Keyword(s):  
Gel Electrophoresis ◽  
Genomic Dna ◽  
Dna Isolation ◽  
Genomic Dna Isolation ◽  
D1s80 Locus
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