scholarly journals New record of Grateloupia taiwanensis S.-M. Lin et H.-Y. Liang in Vietnam: Evidence of morphological observation and rbcL sequence analysis

2019 ◽  
Vol 20 (3) ◽  
pp. 688-695 ◽  
Author(s):  
XUAN-VY XUAN-NGUYEN ◽  
TRUNG-HIEU NGUYEN ◽  
VIET-HA DAO ◽  
LAWRENCE LIAO
Keyword(s):  
Sequence Analysis ◽  
New Record ◽  
Marine Algae ◽  
Large Subunit ◽  
Sequence Divergence ◽  
Morphological Observation ◽  
Species Classification ◽  
Bisphosphate Carboxylase ◽  
Rbcl Sequence ◽  
First Time

Abstract. Nguyen XV, Nguyen TH, Dao VH, Liao L. 2019. New record of Grateloupia taiwanensis S.-M. Lin et H.-Y. Liang in Vietnam: Evidence of morphological observation and rbcL sequence analysis. Biodiversitas 20: 688-695. Members of Grateloupia show highly diverse morphological traits, and this makes species classification more difficult.  Samples were found growing with other marine algae nearshore, 3–5 m depth at Da Nang City. Morphological observation of vegetative and reproductive structures as well as phylogenetic analysis based on the large subunit of ribulose-1,5-bisphosphate-carboxylase-oxygenase (rbcL) sequence confirmed its identification.  Phylogeny of members of Grateloupia inferred from Bayesian Inference, Maximum Likelihood, Maximum Parsimony and Neighbour Joining indicated materials collected in Vietnam compared well with known G. taiwanensis from the type locality, with very little sequence divergence. Grateloupia taiwanensis S.-M. Lin et H.-Y. Liang is therefore reported for the first time from Vietnam.

scholarly journals Selection of suitable fragment from rbcL gene for DNA barcode analysis of family Halymeniaceae, Rhodophyta

2019 ◽  
Vol 19 (4A) ◽  
pp. 201-213
Author(s):  
Nguyen Xuan Vy ◽  
Nguyen Nhat Nhu Thu ◽  
Nguyen Trung Hieu ◽  
Nguyen Thi Xuan Thuy
Keyword(s):  
Phylogenetic Analysis ◽  
Phylogenetic Trees ◽  
Large Subunit ◽  
Dna Barcode ◽  
Full Length ◽  
Species Classification ◽  
Gene Encoding ◽  
Bisphosphate Carboxylase ◽  
Marine Algal ◽  
The Cost

Among the members of Halymeniaceae family, Grateloupia sensu lato occupies the largest composition in species. Classification based on morphological traits is difficult due to the highly variable terete to blade-like thalli among the members of this genus that usually leads to misidentification. Molecular systematics has been applied to classify Grateloupia sensu lato so that the taxonomists acquire a better understanding of the species diversity in general. The plastid gene encoding the large subunit of ribulose-1,5-bisphosphate-carboxylase-oxygenase (rbcL) was the focus of numerous marine algal studies concerning phylogeny and molecular evolution. However, using the full length of rbcL showed disadvantages such as cost and time consuming due to two times of sequencing and two times of PCR. In the present study, the shorter sequence, fragment 773 bp at 5’ end and fragment 579 bp at 3’ end of rbcL were applied and compared for the phylogenetic analysis of Halymeniaceae members. The results indicated there are no differences of topological phylogenetic trees, species resolution within genus and genus resolution within the family between fragment 773 bp at 5’ and the full length of rbcL. Therefore, we conclude that fragment 773 bp at 5’ should be used as DNA barcodes for the Halymeniaceae to reduce the cost and time during phylogenetic analysis. Two taxa Grateloupia newly collected in Vietnam were grouped to the known Phyllymenia, a new genus in Vietnam.

The Ribulose Bisphosphate Carboxylase/Oxygenase of Prochlorothrix hollandica: Purification, Subunit Structure and Partial N-Terminal Sequence Analysis of the Large Subunit

1990 ◽  
Vol 45 (7-8) ◽  
pp. 733-738
Author(s):  
A.M. Hawthornthwaite ◽  
T. J. Beanland ◽  
C. J. Howe ◽  
G. A. Codd
Keyword(s):  
Sequence Analysis ◽  
Quaternary Structure ◽  
Large Subunit ◽  
Subunit Structure ◽  
Ribulose Bisphosphate Carboxylase ◽  
Terminal Sequence ◽  
Bisphosphate Carboxylase ◽  
Apparent Homogeneity ◽  
Ribulose Bisphosphate Carboxylase Oxygenase ◽  
High Degree

Abstract Ribulosc bisphosphate carboxylase/oxygenase was purified to apparent homogeneity from the carboxysomes of Prochlorothrix hollandica.The MW of the native enzyme was estimated to be 560,000 Dalton, comprising large subunits (LSU) of 57,000 Dalton and small subunits (SSU) of 13,000, probably in an 8LSU8SSU quaternary structure. Enzyme activity was maximal at pH 8.0 at 30°C. The requirement of activity for Mg2+ could not be replaced by Mn2+, Co2+, Ca2+ or Cu2+.Amino acid N-terminal sequence analysis of the LSU showed a high degree of conservation when compared to cyanobacterial and chloroplast LSU sequences but was too short to allow a reliable phylogenetic assignment of P. hollandica.

Contribution to the flora of marine algae of the coastal zone of the northeastern part of the Sea of Okhotsk

2018 ◽  
Vol 52 (1) ◽  
pp. 63-73
Author(s):  
N. V. Evseeva
Keyword(s):  
Coastal Zone ◽  
Sea Of Okhotsk ◽  
Marine Algae ◽  
Herbarium Material ◽  
Northeastern Part ◽  
The Sea Of Okhotsk ◽  
The Family ◽  
First Time

The revision of the herbarium material, collected in the northern part of the Sea of Okhotsk in 1965–1966 and stored in VNIRO, allowed to expand the taxonomic list of macrophyte algae of the coastal zone of this area. The locations of discovery of 24 previously unmentioned species are described. Myrionema balticum, Ulvella repens, Syncoryne reinkei, Acrochaetium arcuatum were found in the Sea of Okhotsk for the first time. Most species new for the Sea of Okhotsk is represented by epiphytes of the family Ulvellaceae (Chlorophyta). The final taxonomic list of this region, including literature data, consists of 169 species.

Blank Swift Caltoris kumara moorei (Evans, 1926) (Lepidoptera: Hesperiidae) in Dehradun Valley, Uttarakhand, India: a new record for the western Himalaya

2020 ◽  
Vol 12 (17) ◽  
pp. 17380-17382
Author(s):  
Arun Pratap Singh
Keyword(s):  
New Record ◽  
Western Himalaya ◽  
First Time

The paper provides details of the record of a  butterfly, the Blank Swift Caltoris kumara moorei (Evans, 1926) (Lepidoptera: Hesperiidae), recorded for the first time from Uttarakhand state and the Western Himalaya.

New record of Agrisius Walker (Lepidoptera, Erebidae, Arctiinae) from Cambodia, with description of a new species

Zootaxa ◽  
2021 ◽  
Vol 4915 (4) ◽  
pp. 567-574
Author(s):  
ULZIIJARGAL BAYARSAIKHAN ◽  
TAK-GI LEE ◽  
ANTON V. VOLYNKIN ◽  
URAL YUNUSBAEV ◽  
HYUNG WOOK KWON ◽  
...  
Keyword(s):  
New Species ◽  
New Record ◽  
First Time ◽  
A New Species

The genus Agrisius Walker (1855) is reported for the first time in Cambodia, for the new species Agrisius nigripunctata sp. n. Additionally, Agrisius fuliginosus is reported for the first time in Laos, Myanmar, China, Thailand and Vietnam. Illustrations of the adult and genitalia of Agrisius nigripunctata sp. n. and A. fuliginosus Moore are presented. 

scholarly journals Sarocladium and Lecanicillium Associated with Maize Seeds and Their Potential to Form Selected Secondary Metabolites

Biomolecules ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 98
Author(s):  
Lidia Błaszczyk ◽  
Agnieszka Waśkiewicz ◽  
Karolina Gromadzka ◽  
Katarzyna Mikołajczak ◽  
Jerzy Chełkowski
Keyword(s):  
Large Subunit ◽  
Solid Substrate ◽  
Rrna Gene ◽  
Decenoic Acid ◽  
Bioactive Substances ◽  
Lecanicillium Lecanii ◽  
Maize Seeds ◽  
Internally Transcribed Spacer ◽  
First Time

The occurrence and diversity of Lecanicillium and Sarocladium in maize seeds and their role in this cereal are poorly understood. Therefore, the present study aimed to investigate Sarocladium and Lecanicillium communities found in endosphere of maize seeds collected from fields in Poland and their potential to form selected bioactive substances. The sequencing of the internally transcribed spacer regions 1 (ITS 1) and 2 (ITS2) and the large-subunit (LSU, 28S) of the rRNA gene cluster resulted in the identification of 17 Sarocladium zeae strains, three Sarocladium strictum and five Lecanicillium lecanii isolates. The assay on solid substrate showed that S. zeae and S. strictum can synthesize bassianolide, vertilecanin A, vertilecanin A methyl ester, 2-decenedioic acid and 10-hydroxy-8-decenoic acid. This is also the first study revealing the ability of these two species to produce beauvericin and enniatin B1, respectively. Moreover, for the first time in the present investigation, pyrrocidine A and/or B have been annotated as metabolites of S. strictum and L. lecanii. The production of toxic, insecticidal and antibacterial compounds in cultures of S. strictum, S. zeae and L. lecanii suggests the requirement to revise the approach to study the biological role of fungi inhabiting maize seeds.

scholarly journals Multilocus phylogenies reveal three new truffle-like taxa and the traces of interspecific hybridization in Octaviania (Boletaceae, Boletales)

IMA Fungus ◽  
2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Takamichi Orihara ◽  
Rosanne Healy ◽  
Adriana Corrales ◽  
Matthew E. Smith
Keyword(s):  
Rna Polymerase Ii ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Gene Tree ◽  
Phylogenetic Network ◽  
Elongation Factor ◽  
Morphological Observation ◽  
Tree Comparison ◽  
Unknown Species ◽  
The Usa

ABSTRACTAmong many convergently evolved sequestrate fungal genera in Boletaceae (Boletales, Basidiomycota), the genus Octaviania is the most diverse. We recently collected many specimens of Octaviania subg. Octaviania, including several undescribed taxa, from Japan and the Americas. Here we describe two new species in subgenus Octaviania, O. tenuipes and O. tomentosa, from temperate to subtropical evergreen Fagaceae forests in Japan based on morphological observation and robust multilocus phylogenetic analyses (nrDNA ITS and partial large subunit [LSU], translation elongation factor 1-α gene [TEF1] and the largest subunit of RNA polymerase II gene [RPB1]). Based on specimens from the Americas as well as studies of the holotype, we also taxonomically re-evaluate O. asterosperma var. potteri. Our analysis suggests that O. asterosperma var. potteri is a distinct taxon within the subgenus Octaviania so we recognize this as O. potteri stat. nov. We unexpectedly collected O. potteri specimens from geographically widespread sites in the USA, Japan and Colombia. This is the first verified report of Octaviania from the South American continent. Our molecular analyses also revealed that the RPB1 sequence of one O. tenuipes specimen was identical to that of a closely related species, O. japonimontana, and that one O. potteri specimen from Minnesota had an RPB1 sequence of an unknown species of O. subg. Octaviania. Additionally, one O. japonimontana specimen had an unusually divergent TEF1 sequence. Gene-tree comparison and phylogenetic network analysis of the multilocus dataset suggest that these heterogenous sequences are most likely the result of previous inter- and intra-specific hybridization. We hypothesize that frequent hybridization events in Octaviania may have promoted the high genetic and species diversity found within the genus.

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