Short Communication:  In vitro antibacterial activity of essential oils from twelve aromatic plants from East Kalimantan, Indonesia

Abstract. Kuspradini H, Putri AS, Egra S, Yanti. 2019. Short Communication: In vitro antibacterial activity of essential oils from twelve aromatic plants from East Kalimantan, Indonesia. Biodiversitas 20: 2039-2042. In the aim of this works was to investigate the antibacterial activity of twelve essential oils against Streptococcus mutans and Streptococcus sobrinus, oral pathogen causing dental caries. The essential oils were collected by a steam distillation method. Refractive index was measured by using a refractometer. The antibacterial activity of essential oils was determined using an agar well diffusion method. The yields of essential oils varied with the plant species. The steam distillation yielded clear to yellowish essential oils. Refractive indexes of oil were found to be in the range of 1.344 to 1.500. Syzigium sp. appeared to be more abundant in oil (1.54%) than the other plant species, while C. odorata was poorer (0.04%). All of the essential oils tested showed a varied level of inhibition zone (0-53.65 mm) against S. mutans and S. sobrinus. The oil from leaves of Cymbopogon citratus had the highest activity against S. sobrinus and S. mutans (53.15 and 52.85 mm, respectively). On the other hand, the Magnolia x alba oil showed the lowest activity against S. mutans and S. sobrinus (10.50 and 11.65 mm, respectively). The research results demonstrated that the essential oil in this study has the potency for development of dental health products for preventing and treating oral infections.

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Evaluation of the in vitro antibacterial activity of some essential oils and their blends against Staphylococcus spp. isolated from episodes of sheep mastitis

RENDICONTI LINCEI ◽

10.1007/s12210-021-00991-5 ◽

2021 ◽

Author(s):

Filippo Fratini ◽

Margherita Giusti ◽

Simone Mancini ◽

Francesca Pisseri ◽

Basma Najar ◽

...

Keyword(s):

Antibacterial Activity ◽

Essential Oils ◽

Diffusion Method ◽

Minimal Bactericidal Concentration ◽

Coagulase Negative Staphylococci ◽

Disk Diffusion Method ◽

In Vitro Antibacterial Activity ◽

High Concentrations ◽

Thymus Serpyllum

AbstractStaphylococcus aureus and coagulase-negative staphylococci are among the major causes of mastitis in sheep. The main goal of this research was to determine the in vitro antibacterial activity of several essential oils (EOs, n 30), then five of them were chosen and tested alone and in blends against staphylococci isolates. Five bacteria were isolated from episodes of ovine mastitis (two S. aureus and three S. xylosus). Biochemical and molecular methods were employed to identify the isolates and disk diffusion method was performed to determine their antimicrobial-resistance profile. The relative percentage of the main constituents in the tested essential oils and their blends was detected by GC-EIMS analysis. Antibacterial and bactericidal effectiveness of essential oils and blends were evaluated through minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC). All of them showed sensitivity to the used antimicrobials. The EOs with the highest antibacterial activity were those belonging to the Lamiaceae family characterized by high concentrations of thymol, carvacrol and its precursor p-cymene, together with cinnamon EO, rich in cinnamaldehyde. In terms of both MIC and MBC values, the blend composed by Thymus capitatus EO 40%, Cinnamomum zeylanicum EO 20%, Thymus serpyllum EO 20% and Satureja montana EO 20% was found to be the most effective against all the isolates. Some essential oils appear to represent, at least in vitro, a valid tool against ovine mastitis pathogens. Some blends showed a remarkable effectiveness than the single oils, highlighting a synergistic effect in relation to the phytocomplex.

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In vitro antibacterial activity of plant essential oils against Staphylococcus hyicus and Staphylococcus aureus, the causative agents of exudative epidermitis in pigs

Archives of Microbiology ◽

10.1007/s00203-018-1512-4 ◽

2018 ◽

Vol 200 (7) ◽

pp. 1001-1007 ◽

Cited By ~ 11

Author(s):

Katy Vaillancourt ◽

Geneviève LeBel ◽

Li Yi ◽

Daniel Grenier

Keyword(s):

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Essential Oils ◽

Plant Essential Oils ◽

Causative Agents ◽

In Vitro Antibacterial Activity ◽

And Staphylococcus Aureus

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EVALUATION OF ANTIBACTERIAL ACTIVITY OF ESSENTIAL OIL OF CINNAMOMUM ZEYLANICUM, EUGENIA CARYOPHYLLATA, AND ROSMARINUS OFFICINALIS AGAINST STREPTOCOCCUS ORALIS

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10i5.17072 ◽

2017 ◽

Vol 10 (5) ◽

pp. 410 ◽

Cited By ~ 1

Author(s):

Samira Hsaine ◽

Reda Charof ◽

Khadija Ounine

Keyword(s):

Antibacterial Activity ◽

Essential Oil ◽

Essential Oils ◽

Rosmarinus Officinalis ◽

Diffusion Method ◽

Cinnamomum Zeylanicum ◽

Streptococcus Oralis ◽

In Vitro Antibacterial Activity ◽

Broth Dilution

Objective: Streptococcus oralis plays an important role in the biofilm formation of dental plaque and the occurrence of periodontal disease. Thepresent study was conducted to evaluate in vitro antibacterial activity of three essential oils, namely, Cinnamomum zeylanicum, Eugenia caryophyllata,and Rosmarinus officinalis against S. oralis.Methods: The antibacterial activity of essential oils was investigated by diffusion method using sterile discs (or aromatograms). The minimuminhibitory concentration (MIC) of essential oils showing important antibacterial activity was measured using the broth dilution method.Results: Evaluation of the antibacterial activity of three essential oils as determined by the aromatogram technique showed that the essential oilof R. officinalis had no effect on S. oralis, while the latter was extremely sensitive to the other two essential oils, but with a higher efficiency of theessential oil of C. zeylanicum (42 mm diameter) than E. caryophyllata (20 mm diameter). Similarly, the MIC and minimum bactericidal concentration(MBC) were higher for the essential oil of C. zeylanicum than the essential oil of E. caryophyllata. The MBC/MIC ratio is of the order of 2. The essentialoils studied therefore appear to exert bactericidal activity against S. oralis.Conclusion: The findings suggest that essential oils of C. zeylanicum and E. caryophyllata may be used as an alternative to synthetic antibiotics.Keywords: Essential oil, Cinnamomum zeylanicum, Eugenia caryophyllata, Rosmarinus officinalis, Antimicrobial activity, Streptococcus oralis.

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Plant essential oils against bacteria isolated from fish: an in vitro screening and in vivo efficacy of Lippia origanoides

Ciência Rural ◽

10.1590/0103-8478cr20190064 ◽

2019 ◽

Vol 49 (6) ◽

Author(s):

Guerino Bandeira Junior ◽

Carine de Freitas Souza ◽

Matheus Dellaméa Baldissera ◽

Sharine Nunes Descovi ◽

Bibiana Petri da Silveira ◽

...

Keyword(s):

Antibacterial Activity ◽

Essential Oils ◽

Bacterial Infections ◽

Cupressus Sempervirens ◽

Therapeutic Success ◽

Plant Essential Oils ◽

In Vitro Antibacterial Activity ◽

Fish Bacteria

ABSTRACT: The use of natural products, such as essential oils (EOs), is a potential novel approach to treat fish bacterial infections with a lower risk of developing resistance. There has been a number of studies reporting the activity of EOs as those obtained from the species Achyrocline satureioides, Aniba parviflora, Aniba rosaeodora, Anthemis nobilis, Conobea scoparioides, Cupressus sempervirens, Illicium verum, Lippia origanoides, and Melaleuca alternifolia against bacteria. However, there are few studies investigating the effect of these EOs against fish bacteria. Therefore, the aim of this study was to evaluate the in vitro antibacterial activity of EOs against the following fish bacteria, Aeromonas hydrophila, Citrobacter freundii, and Raoultella ornithinolytica. Additionally, the in vivo antibacterial activity of the EO L. origanoides was evaluated against experimentally induced A. hydrophila infection of silver catfish (Rhamdia quelen). The EO of L. origanoides was chosen as it showed the highest in vitro antibacterial activity, with minimum inhibitory concentrations ranging from 0.2 to 0.8 mg mL-1. This EO also presented a therapeutic success of 58.33%, on a 30 day A. hydrophila infection. Therefore, we suggested that the EO of L. origanoides may be a viable alternative as a treatment for A. hydrophila infection.

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