scholarly journals EVALUATION OF ANTIBACTERIAL ACTIVITY OF ESSENTIAL OIL OF CINNAMOMUM ZEYLANICUM, EUGENIA CARYOPHYLLATA, AND ROSMARINUS OFFICINALIS AGAINST STREPTOCOCCUS ORALIS

2017 ◽  
Vol 10 (5) ◽  
pp. 410 ◽  
Author(s):  
Samira Hsaine ◽  
Reda Charof ◽  
Khadija Ounine
Keyword(s):  
Antibacterial Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Rosmarinus Officinalis ◽  
Diffusion Method ◽  
Cinnamomum Zeylanicum ◽  
Streptococcus Oralis ◽  
In Vitro Antibacterial Activity ◽  
Broth Dilution

Objective: Streptococcus oralis plays an important role in the biofilm formation of dental plaque and the occurrence of periodontal disease. Thepresent study was conducted to evaluate in vitro antibacterial activity of three essential oils, namely, Cinnamomum zeylanicum, Eugenia caryophyllata,and Rosmarinus officinalis against S. oralis.Methods: The antibacterial activity of essential oils was investigated by diffusion method using sterile discs (or aromatograms). The minimuminhibitory concentration (MIC) of essential oils showing important antibacterial activity was measured using the broth dilution method.Results: Evaluation of the antibacterial activity of three essential oils as determined by the aromatogram technique showed that the essential oilof R. officinalis had no effect on S. oralis, while the latter was extremely sensitive to the other two essential oils, but with a higher efficiency of theessential oil of C. zeylanicum (42 mm diameter) than E. caryophyllata (20 mm diameter). Similarly, the MIC and minimum bactericidal concentration(MBC) were higher for the essential oil of C. zeylanicum than the essential oil of E. caryophyllata. The MBC/MIC ratio is of the order of 2. The essentialoils studied therefore appear to exert bactericidal activity against S. oralis.Conclusion: The findings suggest that essential oils of C. zeylanicum and E. caryophyllata may be used as an alternative to synthetic antibiotics.Keywords: Essential oil, Cinnamomum zeylanicum, Eugenia caryophyllata, Rosmarinus officinalis, Antimicrobial activity, Streptococcus oralis.

scholarly journals Evaluation of the in vitro antibacterial activity of some essential oils and their blends against Staphylococcus spp. isolated from episodes of sheep mastitis

2021 ◽  
Author(s):  
Filippo Fratini ◽  
Margherita Giusti ◽  
Simone Mancini ◽  
Francesca Pisseri ◽  
Basma Najar ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Essential Oils ◽  
Diffusion Method ◽  
Minimal Bactericidal Concentration ◽  
Coagulase Negative Staphylococci ◽  
Disk Diffusion Method ◽  
In Vitro Antibacterial Activity ◽  
High Concentrations ◽  
Thymus Serpyllum

AbstractStaphylococcus aureus and coagulase-negative staphylococci are among the major causes of mastitis in sheep. The main goal of this research was to determine the in vitro antibacterial activity of several essential oils (EOs, n 30), then five of them were chosen and tested alone and in blends against staphylococci isolates. Five bacteria were isolated from episodes of ovine mastitis (two S. aureus and three S. xylosus). Biochemical and molecular methods were employed to identify the isolates and disk diffusion method was performed to determine their antimicrobial-resistance profile. The relative percentage of the main constituents in the tested essential oils and their blends was detected by GC-EIMS analysis. Antibacterial and bactericidal effectiveness of essential oils and blends were evaluated through minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC). All of them showed sensitivity to the used antimicrobials. The EOs with the highest antibacterial activity were those belonging to the Lamiaceae family characterized by high concentrations of thymol, carvacrol and its precursor p-cymene, together with cinnamon EO, rich in cinnamaldehyde. In terms of both MIC and MBC values, the blend composed by Thymus capitatus EO 40%, Cinnamomum zeylanicum EO 20%, Thymus serpyllum EO 20% and Satureja montana EO 20% was found to be the most effective against all the isolates. Some essential oils appear to represent, at least in vitro, a valid tool against ovine mastitis pathogens. Some blends showed a remarkable effectiveness than the single oils, highlighting a synergistic effect in relation to the phytocomplex.

CHEMICAL PROFILING OF BUCHANANIA LANZAN SPRENG ESSENTIAL OIL AND ITS BIOLOGICAL ACTIVITIES

2020 ◽  
pp. 103-108
Author(s):  
AJITH S ◽  
KRISHNA V ◽  
RAVI KUMAR S ◽  
VINAY KUMAR NM
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Molecular Docking ◽  
Essential Oil ◽  
Essential Oils ◽  
Bioactive Compounds ◽  
Biological Activities ◽  
Dna Gyrase ◽  
Diffusion Method

Objective: The present study was designed to evaluate the chemical composition of the essential oil of Buchanania lanzan Spreng extracted from the seeds and to evaluate in vitro antimicrobial antioxidants and molecular docking studies of the major bioactive compounds of essential oil. Methods: The essential oil was obtained by hydrodistillation of the B. lanzan seeds and analyzed by gas chromatography-mass spectrometry (GC-MS). Antibacterial activity was evaluated against Pseudomonas aeruginosa, Salmonella typhi, Vibrio cholerae, Staphylococcus aureus, and Streptococcus pneumoniae clinical isolates by disk diffusion method and resazurin assay determined the minimum inhibitory concentration. The in vitro antioxidant activity was determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and hydrogen peroxide (H2O2) scavenging assay; the essential oil major bioactive compounds are Androstan-3-ol, Campesterol, and γ-Sitosterol were docked against bacterial protein DNA gyrase. Results: GC-MS analysis exhibited the presence of 19 bioactive compounds. The essential oil showed that significant antibacterial activity was noticed against V. cholerae and S. typhi with the highest zone of inhibition 15.67–1.20 and 13.83–0.33, respectively. Antioxidant activity in DPPH and H2O2 scavenging assays with IC50 values of 134.23 and 191.24, respectively. The molecular docking of Androstan-3-ol and γ-Sitosterol with bacterial DNA gyrase unveiled a good binding affinity of −6.4 kcal/mol and −6.3 kcal/ mol, respectively. Conclusion: It could be concluded that the essential oils potential sources of antibacterial, antioxidant activities, and molecular docking of bioactive components. The results of this study provide partial scientific support for the traditional application of essential oils to cure diarrhea and also major bioactive compounds responsible for important biological activities.

scholarly journals Chemical Composition and Antibacterial Activity of the Essential Oil of Retrohpyllum rospigliosii Fruits from Colombia

2010 ◽  
Vol 5 (7) ◽  
pp. 1934578X1000500
Author(s):  
Clara E. Quijano-Celis ◽  
Mauricio Gaviria ◽  
Consuelo Vanegas-López ◽  
Ina Ontiveros ◽  
Leonardo Echeverri ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Essential Oil ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Bacterial Strains ◽  
Positive Bacterium ◽  
Disc Diffusion Method ◽  
In Vitro Antibacterial Activity ◽  
Bacterium Bacillus

The essential oil from fruits of Retrophyllum rospigliosii (Pilger) C.N. Page grown in Colombia was analyzed by GC and GC/MS. Ninety-one compounds were identified, of which the most prominent were limonene (37.7%) and α-pinene (16.3%). The in vitro antibacterial activity of the essential oil was studied against seven bacterial strains using the disc diffusion method. The strongest activity of the oil was against the Gram-positive bacterium Bacillus cereus.

scholarly journals In VitroAntibacterial Activity of Essential Oils againstStreptococcus pyogenes

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Julien Sfeir ◽  
Corinne Lefrançois ◽  
Dominique Baudoux ◽  
Séverine Derbré ◽  
Patricia Licznar
Keyword(s):  
Antibacterial Activity ◽  
Essential Oils ◽  
Antimicrobial Agents ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Thymus Vulgaris ◽  
Dilution Method ◽  
Origanum Compactum ◽  
Broth Dilution

Streptococcus pyogenesplays an important role in the pathogenesis of tonsillitis. The present study was conducted to evaluate thein vitroantibacterial activities of 18 essential oils chemotypes from aromatic medicinal plants againstS. pyogenes. Antibacterial activity of essential oils was investigated using disc diffusion method. Minimum Inhibitory Concentration of essential oils showing an important antibacterial activity was measured using broth dilution method. Out of 18 essential oils tested, 14 showed antibacterial activity againstS. pyogenes. Among themCinnamomum verum, Cymbopogon citratus, Thymus vulgaris CT thymol, Origanum compactum, andSatureja montanaessential oils exhibited significant antibacterial activity. Thein vitroresults reported here suggest that, for patients suffering from bacterial throat infections, if aromatherapy is used, these essential oils, considered as potential antimicrobial agents, should be preferred.

scholarly journals Evaluation of antibacterial activity of essential oil from Algerian Pistacia lentiscus resin

2017 ◽  
Vol 1 ◽  
pp. 13
Author(s):  
G. Bachir Raho
Keyword(s):  
Antibacterial Activity ◽  
Essential Oils ◽  
Diffusion Method ◽  
Pistacia Lentiscus ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
Agar Disc ◽  
In Vitro Antibacterial Activity ◽  
Microbial Growth Inhibition

The purpose of this research was to evaluate the in vitro antibacterial activity of the essential oils from the resin of Pistacia lentiscus against Staphylococcus aureus (Gram–positive bacteria) and Escherichia coli (Gram–negative bacteria). The agar disc diffusion method was used for microbial growth inhibition at various dilutions of the oils. Results showed that the tested essential oils possess antibacterial activity against S. aureus but inactive on E. coli. These results may have significant implications for the future development of resin oils of P. lentiscus as an antimicrobial agent for the treatment of the infections caused by S. aureus. 

Chemical Composition and Antibacterial Activity of Essential Oils of Three Medicinal Plants from Algerian Semi-Arid Climatic Zone

2018 ◽  
Vol 16 (S1) ◽  
pp. S155-S163 ◽  
Author(s):  
S. Mehalaine ◽  
O. Belfadel ◽  
T. Menasria ◽  
A. Messaili
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Rosmarinus Officinalis ◽  
Salvia Officinalis ◽  
Thymus Algeriensis

The present study was carried out to determine, for the first time, the chemical composition and antibacterial activity of essential oils derived from the aerial parts of three aromatic plants Thymus algeriensis Boiss & Reut, Rosmarinus officinalis L., and Salvia officinalis L. growing under semiarid conditions. The essential oils were chemically analyzed and identified by gas chromatography (GC) and GC/ mass spectrometry (GC/MS) and their antimicrobial activity was individually evaluated against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa using both agar disk diffusion and agar dilution methods. The major constituents of Thymus algeriensis essential oil were identified as camphor (13.62%), 1,8-cineol (6.00%), borneol (5.74%), viridiflorol (4.00%), and linalool (3.93%). For Rosmarinus officinalis essential oil, 48 compounds were characterized, of which the main constituents were camphor (17.09%), Z-β-ocimene (10.88%), isoborneol (9.68%), α-bisabolol (7.89%), and borneol (5.11%). While, Salvia officinalis essential oil was characterized by β-thujone (16.44%), followed by viridiflorol (10.93%), camphor (8.99%), 1,8-cineol (8.11%), trans-caryophyllene (5.85%), and α-humulene (4.69%) as the major components. Notably, results from antibacterial screening indicated that Thymus algeriensis and Salvia officinalis essential oils exhibited a strong inhibitory effect against both Escherichia coli and Staphylococcus aureus compared to Rosmarinus officinalis essential oil. Further, less activity was recorded against Pseudomonas aeruginosa for the three tested essential oils.

scholarly journals ANTIBACTERIAL AND ANTIOXIDANT ACTIVITIES OF STEM BARK ESSENTIAL OIL CONSTITUENTS OF LITSEA GLUTINOSA C. B. ROB.

2016 ◽  
Vol 8 (12) ◽  
pp. 258
Author(s):  
Arunodaya H. S. ◽  
Krishna V. ◽  
Shashikumar R. ◽  
Girish Kumar K.
Keyword(s):  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Stem Bark ◽  
Diffusion Method ◽  
Vibrio Cholera ◽  
Litsea Glutinosa ◽  
Β Carotene ◽  
Mic Value

<p><strong>Objective: </strong>To evaluate the chemical composition, antibacterial and antioxidant properties of stem bark essential oil of <em>Litsea glutinosa </em>C. B. Rob.</p><p><strong>Methods: </strong>The essential oil isolated from stem bark of <em>L. glutinosa </em>and their chemical composition was analyzed by gas chromatography coupled with mass spectrometry detector. The <em>in vitro </em>antibacterial activity of the stem bark essential oil was investigated against eight human pathogenic bacterial clinical isolates using agar disc diffusion method and MIC value was determined by modified resazurin microtitre-plate assay. The antioxidant activity of essential oil was measured by 2, 2-diphenyl-1-picrylhydrazyl radical (DPPH), 2, 2-azinobis-3-ethylbenzothiazoline-6-sulphonate radical cation (ABTS) and β-carotene bleaching assay.</p><p><strong>Results: </strong>GC-MS analysis of stem bark essential oil resulted in the identification of 37 compounds, off which 9,12-octadecadienoic acid (62.57%), hexadecanoic acid (12.68%), stigmast-5-en-3-ol (6.87%) and vitamin E (2.51%) were the main constituents representing 84.63% of the oil. The determination of <em>in vitro</em> antibacterial activity of stem bark essential oil resulted in significant inhibition zone (15.00±0.57 mm) and MIC value (0.15±0.15×10<sup>-2</sup> mg/ml) against the pathogenic bacteria <em>Vibrio cholera</em> followed by <em>Pseudomonas aeruginosa</em> and <em>Salmonella typhi. </em>The results of DPPH radical scavenging (IC<sub>50</sub>:4.540±0.06 µg/ml), ABTS (IC<sub>50</sub>:256.02±0.06 µg/ml) and β-carotene bleaching assay (%I: 78.51±0.42 <strong>%</strong>) showed significant <em>in vitro</em> antioxidant property.</p><p><strong>Conclusion: </strong><em>L. glutinosa</em> stem bark essential oil showed potential antibacterial activity against the <em>Vibrio cholera</em>. The results of this investigation supported the ethnomedical claim of essential oil as a demulcent, antidiarrheal and antioxidant drug.</p>

scholarly journals Komposisi Minyak Atsiri dan Aktivitas Antimikroba Rimpang Temu Putih dan Jahe Gajah sebagai Fitobiotik Pakan Unggas

2019 ◽  
Vol 6 (2) ◽  
pp. 181
Author(s):  
Laila Nur Rohma ◽  
Laila Nur Rohma ◽  
Osfar Sjofjan ◽  
M. Halim Natsir
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Feed Additives ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Activity Test ◽  
Main Component

ABSTRAK                                                                        Imbuhan pakan unggas dapat berasal dari bahan herbal yang mengandung berbagai komponen aktif yang bermanfaat bagi pertumbuhan ternak.Temu putih dan jahe gajah dapat dimanfaatkan sebagai imbuhan pakan karena mengandung minyak atsiri yang dapat berperan sebagai agen antibakteri. Penelitian ini bertujuan untuk mengetahui komponen penyusun minyak atsiri dan aktivitas antimikroba pada rimpang temu putih dan jahe gajah. Penelitian dilakukan dengan percobaan in vitro menggunakan temu putih dan jahe gajah yang diolah menjadi bentuk ekstrak minyak atsiri temu putih dan jahe gajah sebagai materi uji komposisi penyusun minyak atsiri serta bentuktepung dan enkapsulasi sebagai materi uji aktivitas antimikroba. Komposisi minyak atsiri temu putih terdiri dari lima komponen penyusun dengan cis-1,7-octadien-3-yl acetat sebagai komponen utama. Komposisi minyak atsiri jahe gajah terdiri dari tujuh komponen dan benzene,1-(1,5-dimethyl-4-hexenyl)-4-methyl-(CAS) ar-curcumene sebagai komponen utama. Minyak atsiri yang terkandung pada temu putih dan jahe gajah mempunyai peran dalam menghambat mikroba. Uji komposisi penyusun minyak atsiri menggunakan alat GC-MS dan uji aktivitas antimikroba menggunakan metode disc diffusion dan. Hasil dari uji aktivitas antimikroba menunjukkan bahwa temu putih dan jahe gajah dalam bentuk tepung dan enkapsulasi memiliki perbedaan yang sangat nyata (P<0,01) terhadap aktivitas antimikroba pada bakteri asam laktat, Escherichia coli dan Salmonella sp. Campuran temu putih dan jahe gajah (1:1) menunjukkan kemampuan terbaik dalam menghambat pertumbuhan bakteri patogen dengan diameter zona hambat 5,70±0,14 mm  (Escherichia coli) dan 6,88±0,45 mm (Salmonella sp.).Kata Kunci : antimikroba, fitobiotik, jahe gajah, minyak atsiri, temu putihABSTRACTThe poultry feed additives can contain herbal ingredients that contain various beneficial components for livestock growth. White turmeric and giant ginger can be used as feed additives because they contain essential oils that can be used as antibacterial agents. This study aims to determine the constituent components of essential oils and antimicrobial activity in white turmeric and giant ginger rhizomes. The study was carried out by in vitro experiments using white turmeric and giant ginger which were processed into the form of essential oil extract as material for the composition of essential oils test, and powder and encapsulation form as antimicrobial activity test material. The composition of essential oils of white turmeric consists of five constituent components with cis-1,7-octadien-3-yl acetate as the main component. The composition of giant ginger essential oil consists of seven components with benzene, 1- (1,5-dimethyl-4-hexenyl) -4-methyl- (CAS) ar-curcumene as the main component. Essential oils contained in the white turmeric and giant ginger have a role in inhibiting microbes. The composition of the essential oil tested using GC-MS and the antimicrobial activity test used the disc diffusion method. The results of the antimicrobial activity test showed that white turmeric and giant ginger in powder and encapsulation form had significant differences (P <0.01) on antimicrobial activity in lactic acid bacteria, Escherichia coli and Salmonella sp. The mixture of white turmeric and giant ginger (1: 1) showed the best ability to inhibit the growth of pathogenic bacteria with inhibitory zone diameters of 5.70 ± 0.14 mm (Escherichia coli) and 6.88 ± 0.45 mm (Salmonella sp.).Keywords: antimicrobial, essential oil, giant ginger, phytobiotic, white turmeric

scholarly journals In vitro Antibacterial Activity of Organic Extracts of Aloe barbadensis against Multi-drug resistant Pseudomonas aeruginosa Isolated from Wound Specimens

2018 ◽  
Vol 5 ◽  
pp. 69-76
Author(s):  
Mamata Adhikari ◽  
Anil Kumar Sah ◽  
Dev Raj Joshi
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Inhibitory Action ◽  
Therapeutic Option ◽  
Diffusion Method ◽  
Drug Resistant ◽  
Aloe Barbadensis ◽  
Organic Extracts ◽  
In Vitro Antibacterial Activity

Objectives: In order to investigate alternate therapeutic option, this study was carried out to assess the in vitro antibacterial activity of gel extract of Aloe barbadensis against multiple antibiotic resistant Pseudomonas aeruginosa isolated from wound specimens. Methods: A total of 180 different wound specimens collected in a hospital, were subjected to isolate and identify P. aeruginosa by cultural methods. Antibiotic susceptibility testing was done by Kirby- Bauer disc diffusion method to screen multidrug resistant isolates. A. barbadensis extracts were prepared using aqueous and organic solvents and their in vitro inhibitory action was evaluated by agar well diffusion methods. Results: Out of total, 38 (21.1%) of the wound specimens showed the occurrence of P. aeruginosa, among which 15 (39%) isolates were multi-drug resistant. Organic extracts of various concentrations (0.2 - 0.8 v/v %) inhibited 66.7% of MDR and all non-MDR (n = 23) P. aeruginosa with zone of inhibition ranging from 9.5 ±1.0 to 21.3 ± 2.2 mm but not by aqueous extract. A positive Pearson’s correlation (r=0.983) was found between antibacterial effect and concentrations of the extracts. The antibacterial activity of organic extracts was statistically associated with antibiotic resistance profile of the organism (p<0.05). Conclusion: Organic extracts of A. barbadensis revealed variable in vitro inhibitory action against both MDR and non-MDR P. aeruginosa isolated from wound specimens. Although further confirmation is needed, aloe gel extract may be applied as an alternate treatment option.

scholarly journals CHEMICAL COMPOSITION OF ESSENTIAL OILS FROM VITEX AGNUS-CASTUS L. GROWING IN MOROCCO AND ITS IN VITRO ANTIBACTERIAL ACTIVITY AGAINST CLINICAL BACTERIA RESPONSIBLE FOR NOSOCOMIAL INFECTIONS

2018 ◽  
Vol 11 (10) ◽  
pp. 365 ◽  
Author(s):  
Fatima El Kamari ◽  
Amal Taroq ◽  
Yassine El Atki ◽  
Imane Aouam ◽  
Badiaa Lyoussi ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oils ◽  
Nosocomial Infections ◽  
University Hospital ◽  
Mass Spectrometry Analysis ◽  
Diffusion Method ◽  
Strong Activity ◽  
Agnus Castus

Objective: The aim of the current study is to determine the chemical composition and evaluate antibacterial activity of Vitex agnus-castus L. (VAC) essential oils against some bacteria causing nosocomial infections in the neonatal and intensive care rooms at the university hospital center of Fez Morocco. Methods: The phytochemical screening of essential oils was determined using gas chromatography (GC) and GC-mass spectrometry analysis. The antibacterial test was evaluated against Gram-positive bacteria Staphylococcus aureus and Gram-negative bacteria species (Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Proteus mirabilis) using disc diffusion method. Results: Twenty-nine components were identified in the fruits’ oil representing 93.1% of total oil. The major components in the fruits oil are 1,8-cineole (11.6%), α-thujene (9.3%), phyllocladene (8.2%), α-pinene (7.9%), caryophyllene (5.9%), and cubenol (5%). Furthermore, 28 components were identified in the leaf essential oil. The main component was caryophyllene (9.5%), followed by 1,8-cineole (8.7%), manoyl oxide (7.3%), eugenyl acetate (7.1%), phyllocladene (6.8%), and α-pinene (5.2%). Antibacterial activity of both oils showed a strong activity against nosocomial bacteria tested. Conclusion: Essential oils of Moroccan VAC could be exploited as natural drugs for bacteria, especially those who have acquired resistance to conventional antibiotics.

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