scholarly journals DNA-based analysis of shark products sold on the Indonesian market towards seafood labelling accuracy program

2020 ◽  
Vol 21 (4) ◽  
Author(s):  
Asadatun Abdullah ◽  
MALA NURILMALA ◽  
EFIN MUTTAQIN ◽  
IRFAN YULIANTO
Keyword(s):  
Dna Analysis ◽  
Dna Barcode ◽  
Mitochondrial Genes ◽  
Shark Species ◽  
Cyt B ◽  
Routine Method ◽  
Cytochrome C Oxidase I ◽  
Fish Products ◽  
Fishery Products ◽  
Cyt B Gene

Abstract. Abdullah A, Nurilmala M, Muttaqin E, Yulianto I. 2020. DNA-based analysis of shark products sold on the Indonesian market towards seafood labelling accuracy program. Biodiversitas 21: 1385-1390. Authentication of fishery products has relied mainly on DNA analysis of mitochondrial genes such as cytochrome c oxidase I (COI) DNA barcoding and cytochrome b (cyt b) gene fragments. The trend of sharks and rays food products trading in Indonesia significantly increased, due to their important role as protein source which may threaten the vulnerability status of some species. This study was aimed to determine the reliability of COI mini- DNA barcode and cyt b fragment for identification of shark species of heavily processed fishery products. We found the mini-DNA barcode was an effective tool to identify the shark species traded in local markets with most of the sample identifies as Carcharhinus falciformis. Our results highlight the urgency of applying DNA-based method as a routine method to control the labelling of Indonesian fish products and to manage sustainable fisheries.

scholarly journals Illegal, Unreported, and Unregulated Fisheries Threatening Shark Conservation in African Waters Revealed from High Levels of Shark Mislabelling in Ghana

Genes ◽  
2021 ◽  
Vol 12 (7) ◽  
pp. 1002
Author(s):  
Narkie Akua Agyeman ◽  
Carmen Blanco-Fernandez ◽  
Sophie Leonie Steinhaussen ◽  
Eva Garcia-Vazquez ◽  
Gonzalo Machado-Schiaffino
Keyword(s):  
Public Awareness ◽  
Pelagic Fish ◽  
Genetic Identification ◽  
Coi Gene ◽  
Shark Species ◽  
Cytochrome C Oxidase I ◽  
Fish Products ◽  
Iuu Fishing ◽  
Isurus Oxyrinchus ◽  
Shark Conservation

Mislabelling of fish and fish products has attracted much attention over the last decades, following public awareness of the practice of substituting high-value with low-value fish in markets, restaurants, and processed seafood. In some cases, mislabelling includes illegal, unreported, and unregulated (IUU) fishing, contributing to overexploit substitute species that are undetectable when sold under wrong names. This is the first study of DNA barcoding to assess the level of mislabelling in fish marketed in Ghana, focusing on endangered shark species. Genetic identification was obtained from 650 base pair sequences within the cytochrome c oxidase I (COI) gene. All except one of 17 shark fillets analysed were wrongly labelled as compared with none of 28 samples of small commercial pelagic fish and 14 commercial shark samples purchased in Europe. Several substitute shark species in Ghana are endangered (Carcharhinus signatus and Isurus oxyrinchus) and critically endangered (Squatina aculeata). Shark products commercialized in Europe (n = 14) did not reveal mislabelling, thus specific shark mislabelling cannot be generalized. Although based on a limited number of samples and fish markets, the results that reveal trade of endangered sharks in Ghana markets encourage Ghanaian authorities to improve controls to enforce conservation measures.

Methods for Anisakis simplex detection in fish and fishery products

2018 ◽  
Vol 74 (1) ◽  
pp. 6049-2018
Author(s):  
Kochanowski M. ◽  
Różycki M. ◽  
Dąbrowska J. ◽  
Bilska-Zając E. ◽  
Karamon J. ◽  
...  
Keyword(s):  
Anisakis Simplex ◽  
Frequent Occurrence ◽  
Allergic Reactions ◽  
Fish Products ◽  
Fish Muscles ◽  
Fishery Products ◽  
Fish And Fishery Products ◽  
Target Analyte

Anisakis simplex is a zoonotic nematode which can cause human anisakiasis. Furthermore, A. simplex allergens, even of dead larvae can cause allergic reactions, including anaphylaxis. Due to the frequent occurrence in fish muscles and pathogenicity, A. simplex is a serious danger for fish products consumers. Therefore, it is necessary to examine fish and fish products for the presence of these parasites before placing on the market. The purpose of this paper is review of methods for A. simplex detection in fish and fishery products. These methods differ according to the effectiveness and type of the target analyte. They also have different suitability for examination of matrices with different properties. Moreover this paper presents legislations associated with A. simplex detection. .

scholarly journals Evaluation of mitochondrial genes as DNA barcode for Basidiomycota

2009 ◽  
Vol 9 ◽  
pp. 99-113 ◽  
Author(s):  
AGATHE VIALLE ◽  
NICOLAS FEAU ◽  
MATHIEU ALLAIRE ◽  
MARYNA DIDUKH ◽  
FRANCIS MARTIN ◽  
...  
Keyword(s):  
Dna Barcode ◽  
Mitochondrial Genes

Research Paper DNA barcode identification of fish products from Guiyang markets in southwest China

2022 ◽  
Author(s):  
Qian Tang ◽  
Qi Luo ◽  
Qian Duan ◽  
Lei Deng ◽  
Renyi Zhang
Keyword(s):  
Dna Barcoding ◽  
Molecular Identification ◽  
Fish Consumption ◽  
Southwest China ◽  
Dna Barcode ◽  
Guizhou Province ◽  
Accurate Identification ◽  
Continuous Growth ◽  
Fish Products ◽  
Fresh Frozen

Nowadays, the global fish consumption continues to rise along with the continuous growth of the population, which has led to the dilemma of overfishing of fishery resources. Especially high-value fish that are overfished are often replaced by other fish. Therefore, the accurate identification of fish products in the market is a problem worthy of attention. In this study, full-DNA barcoding (FDB) and mini-DNA barcoding (MDB) used to detect the fraud of fish products in Guiyang, Guizhou province in China. The molecular identification results showed that 39 of the 191 samples were not consistent with the labels. The mislabelling of fish products for fresh, frozen, cooked and canned were 11.70%, 20.00%, 34.09% and 50.00%, respectively. The average kimura 2 parameter distances of MDB within species and genera were 0.27% and 5.41%, respectively; while average distances of FDB were 0.17% within species and 6.17% within genera. In this study, commercial fraud is noticeable, most of the high-priced fish were replaced of low-priced fish with a similar feature. Our study indicated that DNA barcoding is a valid tool for the identification of fish products and that it allows an idea of conservation and monitoring efforts, while confirming the MDB as a reliable tool for fish products.

scholarly journals Genetic characterization and phylogenetic study of Indonesian indigenous catfish based on mitochondrial cytochrome B gene

2020 ◽  
Vol 13 (1) ◽  
pp. 96-103
Author(s):  
Dorothea Vera Megarani ◽  
Herjuno Ari Nugroho ◽  
Zahrah Prawita Andarini ◽  
Yura Dwi Risa B. R. Surbakti ◽  
Rini Widayanti
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Cytochrome B ◽  
Genetic Characterization ◽  
Phylogenetic Study ◽  
Cyt B ◽  
Phylogenetic Structure ◽  
Mitochondrial Cytochrome B ◽  
Sperata Seenghala ◽  
Cyt B Gene

Aim: This study aimed to determine the genetic characterization and phylogenetic structure of Indonesian indigenous catfish using cytochrome B (Cyt B) sequences. Materials and Methods: The genomes of 26 catfishes caught from nine rivers from nine different geographical locations around Indonesia were analyzed. The tissue isolation method was used to isolate the total genome of the fishes. Furthermore, polymerase chain reaction was done to amplify the mtDNA Cyt B using the CytBF and CytBR primers. Following sequencing, the analysis of genetic variation and the phylogenetic relationship was performed using MEGA version X software. Results: Cyt B gene sequencing attained a total of 1139 nucleotides encrypting 379 amino acids for all samples. The ClustalW alignment program using MEGA X software revealed 395 substituted nucleotides, which then translated into 63 amino acid variation sites among all 26 samples. No amino acids in catfish BB were different compared to catfish PM, MP, and KR2,3. Catfish MS had one modified amino acid; KR1 and KS had two different amino acids; BF had 38 different amino acids; EM had 31 different amino acids; and BSBJ had 26 different amino acids compared to catfish BB. The most significant alteration of amino acids was between catfish EM and BF (49 amino acids). Conclusion: Indonesian catfish were divided into five clades based on the Cyt B gene. Samples KR and MP (Sumatra); MS and BB (Kalimantan); and PM (Java) were clustered with Hemibagrus nemurus and Hemibagrus wyckioides (Bagridae family). Samples from Kalimantan (KS) and one sample of KR (KR1) from Sumatra were clustered with Sperata seenghala and Hemibagrus spilopterus (Bagridae family). Samples from Java (BSBJ) were clustered with Pseudolais pleurotaenia (Pangasiidae family). Samples EM (Java) were together with Mystus cavasius (Bagridae family). Samples from West Papua were clustered with Potamosilurus latirostris (Ariidae family).

scholarly journals Kajian Penanda Genetik Gen Sitokrom b DNA Mitokondria Ikan Lais dari Sungai Kampar Riau

2018 ◽  
Vol 10 (1) ◽  
pp. 6
Author(s):  
Roza Elvyra ◽  
Dedy Duryadi Solihin
Keyword(s):  
Species Diversity ◽  
Molecular Marker ◽  
Phylogenetic Relationship ◽  
Cytochrome B ◽  
Mitochondrial Cytochrome ◽  
Cyt B ◽  
Phylogenetic Marker ◽  
Mitochondrial Cytochrome B ◽  
Cyt B Gene

The mitochondrial cytochrome b (cyt-b) gene as a phylogenetic marker of lais fish Kryptopterus schilbeides from Kampar River in Riau has been studied. This is a prelimininary research on the utility of cyt-b gene as a molecular marker to obtain species diversity and phylogenetic relationship among Kryptopterus fishes from Kampar River. The primers of L14841 and H15149 were used to amplify the cyt-b gene. The results showed that K. schilbeides has isoleusine at site-81 and metionine at site-114; K. schilbeides from Kampar River and K. schilbeides from GenBank form a phylogeny cluster at 45% value.

scholarly journals Partial sequence analysis of mitochondrial cytochrome B gene of Labeo calbasu of Bangladesh

2019 ◽  
Vol 5 (1) ◽  
pp. 25-30
Author(s):  
RA Begum ◽  
MT Alam ◽  
H Jahan ◽  
MS Alam
Keyword(s):  
Genetic Diversity ◽  
Cytochrome B ◽  
Tissue Sample ◽  
Sequence Similarity ◽  
Cytochrome B Gene ◽  
Mitochondrial Cytochrome ◽  
Cyt B ◽  
Multiple Sequence ◽  
Mitochondrial Cytochrome B ◽  
Cyt B Gene

Labeo calbasu (Family Cyprinidae) was studied at DNA level to know genetic diversity within and between species. The mitochondrial cytochrome b (cyt-b) gene of L. calbasu was sequenced and compared to the corresponding sequences of other Labeo species. DNA was isolated from the tissue sample of L. calbasu using phenol: chloroform extraction method. Forward and reverse primers were designed to amplify the target region of cytochrome b gene. A standard PCR protocol was used for the amplification of the desired region. Then, the forward and reverse sequences obtained were aligned and edited to finalize a length of 510 nucleotides which was submitted to NCBI genbank database. Nucleotide BLAST of this sequence at NCBI resulted 100% sequence similarity with L. calbasu sequence of the same region of cyt-b gene. Multiple sequence alignment of the sequence with seven more Labeo species sequences revealed 120 polymorphic sites, which have been mark of diversity among the species and might be used in molecular identification of the Labeo species. A constructed phylogenetic tree has shown relationship among the Labeo species. This research demonstrated the usefulness of mitochondrial DNA-based approach in species identification. Further, the data will provide appropriate background for studying genetic diversity within-species of the Labeo species in general and of L. calbasu in particular. J. Biodivers. Conserv. Bioresour. Manag. 2019, 5(1): 25-30

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