scholarly journals Isolation and identification of indigenous Arbuscular Mycorrhizal Fungi (AMF) of forest clove rhizosphere from Maluku, Indonesia

2021 ◽  
Vol 22 (8) ◽  
Author(s):  
Asri Subkhan Mahulette ◽  
Anggra Alfian ◽  
ABDUL KARIM KILKODA ◽  
IMELDA JEANETTE LAWALATA ◽  
DESSY ARIYANI MARASABESSY ◽  
...  
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal ◽  
Soil Samples ◽  
Spore Density ◽  
Wild Type ◽  
Isolation And Identification ◽  
Spore Count ◽  
Spore Number ◽  
Total Spore Count

Abstract. Mahulette AS, Alfian A, Kilkoda KA, Lawalata IJ, Marasabessy DA, Tanasale VL, Makaruku MH. 2021. Isolation and identification of indigenous Arbuscular Mycorrhizal Fungi (AMF) of forest clove rhizosphere from Maluku, Indonesia. Biodiversitas 22: 3613-3619. Forest clove is classified as wild-type and endemic to the Maluku (Moluccas) Islands, Indonesia. The different condition of growing areas causes various types of Arbuscular Mycorrhizal Fungi (AMF) associated with forest clove. The study aimed to identify and obtain indigenous AMF inoculums from the forest clove rhizosphere from two distribution areas in Maluku. The results of AMF identification found two types of spores from the genus Glomus in the rhizosphere of forest cloves from Ambon Island with a spore density of 35/50 g of soil. In comparison, three spores were found in Seram Island, two from the genus Scutellospora and one from the Acaulospora. With an overall spore density of 5/50 g of soil. After culture trapping, there was a change in type and an increase in spore density in soil samples from the rhizosphere of the two forest clove distribution areas. Soil samples from Ambon after trapping culture obtained two new types of spores from the genus Acaulospora with a total spore number of 57/50 g soil while in soil samples from Seram found three new types of spores from the genus Glomus with a total spore count of 104/50 g of soil.

scholarly journals EKSPLORASI FUNGI MIKORIZA ARBUSKULA (FMA) PADA RIZOSFIR HIJAUAN PAKAN

ZOOTEC ◽  
2017 ◽  
Vol 37 (1) ◽  
pp. 167
Author(s):  
Rifa E. Ansiga ◽  
A. Rumambi ◽  
D. A. Kaligis ◽  
I. Mansur ◽  
W. Kaunang
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
Gliricidia Sepium ◽  
Soil Samples ◽  
Isolation And Identification ◽  
Different Types ◽  
Forage Type ◽  
The Difference

EXPLORATION OF ARBUSCULAR MYCORRHIZAL (AM) FUNGI IN FORAGE RHIZOSPHERES. This study aimed to determine the diversity of Arbuscular Mycorrhizal Fungi (AMF) in several kinds of hybrid forages Rhizospheres, either in grasses or legumes. Soil samples were taken from three different locations, consisted of: Mapanget (forages type: Leucaena leucocepala, Sorghum varieties numbu, Penicettum purpureum cv. Mott), Tateli (forages type: calothyrsus Calliandra, Gliricidia sepium) and Campus of UNSRAT, Manado (forage type: King grass). The soil samples which taken from forages rhizospheres were sieved using Brundrett method and then centrifuged.  Thereafter, isolation and identification of spore were carried out based on spore morphology character, involves: shape, size, color, hyphae attachment, and ornament. Extraction and identification of spores on six types of rhizosphere were found 34 different types of AMF spores in shape and color. In grass, it was found three types of spores, i.e.: Glomus, Acaulospora, and Sclerocystis, meanwhile in leguminous just one type of spore was found, i.e.: Glomus. The difference of rhizosphere in grass and leguminosae resulted in different types of spores, where Sclerocystis and Acaulospora are found in grasses, on the contrary Sclerocystis and Acaulospora are not found in leguminosae. Based on the number of spores of AMF, it seemed that Glomus types found to have the most number, while Sclerocystis and Acaulospora had the lowest number of spores found. Key words: Exploration, Arbuscular Mycorrhizal Fungi (AMF), Grass, Legume, Spores

scholarly journals Response of Arbuscular Mycorrhizal Fungi to Simulated Climate Changes by Reciprocal Translocation in Tibetan Plateau

2015 ◽  
Vol 43 (2) ◽  
pp. 488-493
Author(s):  
Zhaoyong SHI ◽  
Xubin YIN ◽  
Bede MICKAN ◽  
Fayuan WANG ◽  
Ying ZHANG ◽  
...  
Keyword(s):  
Tibetan Plateau ◽  
Arbuscular Mycorrhizal Fungi ◽  
Reciprocal Translocation ◽  
Mycorrhizal Fungi ◽  
Climate Changes ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
Spore Density ◽  
The Tibetan Plateau ◽  
Colonization Frequency

Arbuscular mycorrhiza (AM) fungi are considered as an important factor in predicting plants and ecosystem responses to climate changes on a global scale. The Tibetan Plateau is the highest region on Earth with abundant natural resources and one of the most sensitive region to climate changes. To evaluate the complex response of arbuscular mycorrhizal fungi colonization and spore density to climate changes, a reciprocal translocation experiment was employed in Tibetan Plateau. The reciprocal translocation of quadrats to AM colonization and spore density were dynamic. Mycorrhizal colonization frequency presented contrary changed trend with elevations of quadrat translocation. Colonization frequency reduced or increased in majority quadrats translocated from low to high or from high to low elevation. Responses of colonization intensity to translocation of quadrats were more sensitive than colonization frequency. Arbuscular colonization showed inconsistent trend in increased or decreased quadrat. Vesicle colonization decreased with changed of quadrat from low to high elevations. However, no significant trend was observed. Although spore density was dynamic with signs of decreasing or increasing in translocated quadrats, the majority enhanced and declined respectively in descent and ascent quadrat treatments. It is crucial to understand the interactions between AM fungi and prairie grasses to accurately predict effects of climate change on these diverse and sensitive ecosystems. This study provided an opportunity for understanding the effect of climate changes on AM fungi.

scholarly journals Community Analysis of Arbuscular Mycorrhizal Fungi in Roots ofPoncirus trifoliataandCitrus reticulataBased on SSU rDNA

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Peng Wang ◽  
Yin Wang
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Glomus Intraradices ◽  
Arbuscular Mycorrhizal ◽  
Small Subunit ◽  
Poncirus Trifoliata ◽  
Spore Density ◽  
Morphological Observation ◽  
Trifoliate Orange ◽  
Hyphal Length

Morphological observation of arbuscular mycorrhizal fungi (AMF) species in rhizospheric soil could not accurately reflect the actual AMF colonizing status in roots, while molecular identification of indigenous AMF colonizing citrus rootstocks at present was rare in China. In our study, community of AMF colonizing trifoliate orange (Poncirus trifoliataL. Raf.) and red tangerine (Citrus reticulataBlanco) were analyzed based on small subunit of ribosomal DNA genes. Morphological observation showed that arbuscular mycorrhizal (AM) colonization, spore density, and hyphal length did not differ significantly between two rootstocks. Phylogenetic analysis showed that 173 screened AMF sequences clustered in at least 10 discrete groups (GLO1~GLO10), all belonging to the genus ofGlomusSensu Lato. Among them, GLO1 clade (clustering with uncultured Glomus) accounting for 54.43% clones was the most common in trifoliate orange roots, while GLO6 clade (clustering withGlomus intraradices) accounting for 35.00% clones was the most common in red tangerine roots. Although, Shannon-Wiener indices exhibited no notable differences between both rootstocks, relative proportions of observed clades analysis revealed that composition of AMF communities colonizing two rootstocks varied severely. The results indicated that native AMF species in citrus rhizosphere had diverse colonization potential between two different rootstocks in the present orchards.

scholarly journals Diversity of arbuscular mycorrhizal fungi (amf) in three agroecosystems with grass colosoana (Bothriochloa pertusa (L) A. Camus) in the department of Sucre

2016 ◽  
Vol 8 (1) ◽  
pp. 72
Author(s):  
FREDIS ESPITIA D ◽  
ALEXANDER PEREZ C
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal ◽  
Soil Samples ◽  
Compacted Soils ◽  
San Marcos

The present study evaluated the diversity of arbuscular mycorrhizal fungi (AMF) associated soils from three ecosystems grown with pasture colosoana in three localities of the Department of Sucre, Colombia. Soil samples were collected, AMF spores isolated, count of spore was made and morphotypes isolated were classification at the level of species or genera. We found that the genus of AMF over prevailing in native and compacted soils in the three selected municipalities is the genus Glomus, followed by other genera to a lesser extent. In general the soils that presented greater wealth of genres of HMA were those of the municipality of San Marcos, while the lowest richness was found in the municipality of Sincelejo.

scholarly journals Impact of Land Use Intensity on the Species Diversity of Arbuscular Mycorrhizal Fungi in Agroecosystems of Central Europe

2003 ◽  
Vol 69 (5) ◽  
pp. 2816-2824 ◽  
Author(s):  
Fritz Oehl ◽  
Ewald Sieverding ◽  
Kurt Ineichen ◽  
Paul Mäder ◽  
Thomas Boller ◽  
...  
Keyword(s):  
Land Use ◽  
Arbuscular Mycorrhizal Fungi ◽  
Crop Rotation ◽  
Mycorrhizal Fungi ◽  
Root Colonization ◽  
Arbuscular Mycorrhizal ◽  
Soil Samples ◽  
Land Use Intensity ◽  
High Input ◽  
Continuous Maize

ABSTRACT The impact of land use intensity on the diversity of arbuscular mycorrhizal fungi (AMF) was investigated at eight sites in the “three-country corner” of France, Germany, and Switzerland. Three sites were low-input, species-rich grasslands. Two sites represented low- to moderate-input farming with a 7-year crop rotation, and three sites represented high-input continuous maize monocropping. Representative soil samples were taken, and the AMF spores present were morphologically identified and counted. The same soil samples also served as inocula for “AMF trap cultures” with Plantago lanceolata, Trifolium pratense, and Lolium perenne. These trap cultures were established in pots in a greenhouse, and AMF root colonization and spore formation were monitored over 8 months. For the field samples, the numbers of AMF spores and species were highest in the grasslands, lower in the low- and moderate-input arable lands, and lowest in the lands with intensive continuous maize monocropping. Some AMF species occurred at all sites (“generalists”); most of them were prevalent in the intensively managed arable lands. Many other species, particularly those forming sporocarps, appeared to be specialists for grasslands. Only a few species were specialized on the arable lands with crop rotation, and only one species was restricted to the high-input maize sites. In the trap culture experiment, the rate of root colonization by AMF was highest with inocula from the permanent grasslands and lowest with those from the high-input monocropping sites. In contrast, AMF spore formation was slowest with the former inocula and fastest with the latter inocula. In conclusion, the increased land use intensity was correlated with a decrease in AMF species richness and with a preferential selection of species that colonized roots slowly but formed spores rapidly.

Yield and Rhizosphere - microflora of Cowpea in Response to Magnesium Fertilization in Lateritic Soils of Kerala

2021 ◽  
Author(s):  
V.P. Soniya ◽  
P.S. Bhindhu
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Root Colonization ◽  
Arbuscular Mycorrhizal ◽  
Nitrogen Fixing ◽  
Nitrogen Fixing Bacteria ◽  
Free Living ◽  
Spore Count ◽  
Lateritic Soils ◽  
Magnesium Uptake

Background: Magnesium deficiency has become a major nutritional disorder in lateritic soils of Kerala. Appropriate magnesium fertilization is the best strategy to combat deficiency issues. Apart from correcting nutritional deficiency, magnesium fertilization has an influence on the growth of beneficial microbes such as nitrogen fixing bacterias and arbuscular mycorrhizal fungi. The experiment aimed to investigate the effect of magnesium fertilization on crop yield and population rhizosphere micoflora of cowpea in lateritic soils of Kerala.Methods: A pot culture experiment was conducted with a gradient of magnesium additions ranging from 5 mg kg-1 to 80 mg kg-1 of soil along with recommended dose of fertilizers. Population of rhizobium, free living nitrogen fixing bacteria, spore count of arbuscular mycorrhizal fungi and per cent root colonization of arbuscular mycorrhizal fungi were studied during flowering. The available magnesium and magnesium uptake were also worked out during harvest. Yield and yield contributing characteristics of cowpea were measured during harvest stage.Result: Magnesium addition produced significant variations in population of rhizobium and free- living nitrogen fixing bacteria whereas spore count of AMF and per cent root colonization of AMF did not vary according to the added doses of magnesium. A higher population of rhizobium, free living nitrogen fixers, root nodules, magnesium uptake, plant height and yield were obtained in the treatment where magnesium was applied @ 10 mg kg-1 soil.

scholarly journals Arbuscular Mycorrhizal Fungi Colonization in the Rhizosphere of Aspilia pruliseta Schweif. ext Schweif in the Semiarid Eastern Kenya

2020 ◽  
Vol 13 ◽  
pp. 117862212096919
Author(s):  
James Peter Muchoka ◽  
Daniel Njiru Mugendi ◽  
Paul Nthakanio Njiruh ◽  
Charles Onyari ◽  
Paul Kamau Mbugua ◽  
...  
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Sandy Loam ◽  
Soil Depth ◽  
Arbuscular Mycorrhizal ◽  
Phosphate Deficiency ◽  
Similar Amount ◽  
Negative Consequences ◽  
Spore Count ◽  
Biological Technique

The use of arbuscular mycorrhizal fungi (AMF) to enhance soil phosphate uptake is a biological technique considered to cure phosphate deficiency in soils. This study investigated association of Aspilia pruliseta Schweif shrub with AMF in Kenya. The study aims at profiling a tropical shrub with multiple ecological benefits that could reduce addition of chemical phosphatic fertilizer into the soil and reverse negative consequences of eutrophication. Sampling was purposive to have areas with or without Aspilia pruliseta vegetation growing. A small amount (10 g) of the soil from 27 composite samples was used for spore count determination and a similar amount for next generation sequencing. Spore counts varied significantly among soil textural types, sample locations, and soil depth. Sandy loam had the highest spore counts with a mean average of 404 spores. The spore count decreased significantly ( P < .05) with the depth of soil from a mean of 514 spores to 185 along the rhizosphere. The intensity of spore morphotypes was significantly higher at P < .05 for soils whose vegetation was covered with Aspilia pruliseta than those without. Aspilia pruliseta vegetation used together with sandy loam soil could culture commercial mycorrhiza fungi production for use in agrisystems.

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