scholarly journals Diversity of arbuscular mycorrhizal fungi (amf) in three agroecosystems with grass colosoana (Bothriochloa pertusa (L) A. Camus) in the department of Sucre

2016 ◽  
Vol 8 (1) ◽  
pp. 72
Author(s):  
FREDIS ESPITIA D ◽  
ALEXANDER PEREZ C
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal ◽  
Soil Samples ◽  
Compacted Soils ◽  
San Marcos

The present study evaluated the diversity of arbuscular mycorrhizal fungi (AMF) associated soils from three ecosystems grown with pasture colosoana in three localities of the Department of Sucre, Colombia. Soil samples were collected, AMF spores isolated, count of spore was made and morphotypes isolated were classification at the level of species or genera. We found that the genus of AMF over prevailing in native and compacted soils in the three selected municipalities is the genus Glomus, followed by other genera to a lesser extent. In general the soils that presented greater wealth of genres of HMA were those of the municipality of San Marcos, while the lowest richness was found in the municipality of Sincelejo.

scholarly journals Isolation and identification of indigenous Arbuscular Mycorrhizal Fungi (AMF) of forest clove rhizosphere from Maluku, Indonesia

2021 ◽  
Vol 22 (8) ◽  
Author(s):  
Asri Subkhan Mahulette ◽  
Anggra Alfian ◽  
ABDUL KARIM KILKODA ◽  
IMELDA JEANETTE LAWALATA ◽  
DESSY ARIYANI MARASABESSY ◽  
...  
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal ◽  
Soil Samples ◽  
Spore Density ◽  
Wild Type ◽  
Isolation And Identification ◽  
Spore Count ◽  
Spore Number ◽  
Total Spore Count

Abstract. Mahulette AS, Alfian A, Kilkoda KA, Lawalata IJ, Marasabessy DA, Tanasale VL, Makaruku MH. 2021. Isolation and identification of indigenous Arbuscular Mycorrhizal Fungi (AMF) of forest clove rhizosphere from Maluku, Indonesia. Biodiversitas 22: 3613-3619. Forest clove is classified as wild-type and endemic to the Maluku (Moluccas) Islands, Indonesia. The different condition of growing areas causes various types of Arbuscular Mycorrhizal Fungi (AMF) associated with forest clove. The study aimed to identify and obtain indigenous AMF inoculums from the forest clove rhizosphere from two distribution areas in Maluku. The results of AMF identification found two types of spores from the genus Glomus in the rhizosphere of forest cloves from Ambon Island with a spore density of 35/50 g of soil. In comparison, three spores were found in Seram Island, two from the genus Scutellospora and one from the Acaulospora. With an overall spore density of 5/50 g of soil. After culture trapping, there was a change in type and an increase in spore density in soil samples from the rhizosphere of the two forest clove distribution areas. Soil samples from Ambon after trapping culture obtained two new types of spores from the genus Acaulospora with a total spore number of 57/50 g soil while in soil samples from Seram found three new types of spores from the genus Glomus with a total spore count of 104/50 g of soil.

scholarly journals Impact of Land Use Intensity on the Species Diversity of Arbuscular Mycorrhizal Fungi in Agroecosystems of Central Europe

2003 ◽  
Vol 69 (5) ◽  
pp. 2816-2824 ◽  
Author(s):  
Fritz Oehl ◽  
Ewald Sieverding ◽  
Kurt Ineichen ◽  
Paul Mäder ◽  
Thomas Boller ◽  
...  
Keyword(s):  
Land Use ◽  
Arbuscular Mycorrhizal Fungi ◽  
Crop Rotation ◽  
Mycorrhizal Fungi ◽  
Root Colonization ◽  
Arbuscular Mycorrhizal ◽  
Soil Samples ◽  
Land Use Intensity ◽  
High Input ◽  
Continuous Maize

ABSTRACT The impact of land use intensity on the diversity of arbuscular mycorrhizal fungi (AMF) was investigated at eight sites in the “three-country corner” of France, Germany, and Switzerland. Three sites were low-input, species-rich grasslands. Two sites represented low- to moderate-input farming with a 7-year crop rotation, and three sites represented high-input continuous maize monocropping. Representative soil samples were taken, and the AMF spores present were morphologically identified and counted. The same soil samples also served as inocula for “AMF trap cultures” with Plantago lanceolata, Trifolium pratense, and Lolium perenne. These trap cultures were established in pots in a greenhouse, and AMF root colonization and spore formation were monitored over 8 months. For the field samples, the numbers of AMF spores and species were highest in the grasslands, lower in the low- and moderate-input arable lands, and lowest in the lands with intensive continuous maize monocropping. Some AMF species occurred at all sites (“generalists”); most of them were prevalent in the intensively managed arable lands. Many other species, particularly those forming sporocarps, appeared to be specialists for grasslands. Only a few species were specialized on the arable lands with crop rotation, and only one species was restricted to the high-input maize sites. In the trap culture experiment, the rate of root colonization by AMF was highest with inocula from the permanent grasslands and lowest with those from the high-input monocropping sites. In contrast, AMF spore formation was slowest with the former inocula and fastest with the latter inocula. In conclusion, the increased land use intensity was correlated with a decrease in AMF species richness and with a preferential selection of species that colonized roots slowly but formed spores rapidly.

scholarly journals Arbuscular Mycorrhizal Fungi Associated with Wati (Piper methysticum), a Medicinal Plant from Merauke Lowland, Papua, Indonesia

2018 ◽  
Vol 10 (2) ◽  
pp. 260-266
Author(s):  
Suharno Suharno ◽  
Supeni Sufaati ◽  
Verena Agustini ◽  
Rosye H. R. Tanjung
Keyword(s):  
Medicinal Plant ◽  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal ◽  
Culture Method ◽  
Soil Samples ◽  
Local People ◽  
Piper Methysticum ◽  
Root Sample ◽  
Tract Infections

Indonesia is rich of natural resources, including the diversity of agricultural crops and other valuable plants. Many plant species used by local people for traditional herbal medicine and some other species used by drug industries. Among these, Wati (Piper methysticum, Piperaceae) is one of the medicinal plant found in Merauke lowland, Papua. It has been cultivated by local people because of it high value as medicinal and cultural uses. Wati plant is used to treat anti-stress, rheumatism, respiratory tract infections, tuberculosis, gonorrhea, headache etc. The habitat, including the microorganism in the soil plays an important role in the growth of this plant. Therefore, this study was conducted to identify the arbuscular mycorrhizal fungi (AMF) associated with the rhizosphere of Wati from Merauke lowland. Soil and root samples were collected from different locations and the colonization percentage on the root sample were determined. Our results showed that the number of the spores in the soil samples was 45-89 spores/50 g soil, while the colonization percentage on the root was 38.46-83.3%. Among 13 AMF morphospecies that found on the soil samples, 10 were identified to genus level such as Glomus, Clariodeoglomus, Acaulospora and Scutellospora, while the other were unidentified. Further work will be needed such as trap-plant culture method to get more information on the diversity of AMF associated with Wati.

scholarly journals EKSPLORASI FUNGI MIKORIZA ARBUSKULA (FMA) PADA RIZOSFIR HIJAUAN PAKAN

ZOOTEC ◽  
2017 ◽  
Vol 37 (1) ◽  
pp. 167
Author(s):  
Rifa E. Ansiga ◽  
A. Rumambi ◽  
D. A. Kaligis ◽  
I. Mansur ◽  
W. Kaunang
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
Gliricidia Sepium ◽  
Soil Samples ◽  
Isolation And Identification ◽  
Different Types ◽  
Forage Type ◽  
The Difference

EXPLORATION OF ARBUSCULAR MYCORRHIZAL (AM) FUNGI IN FORAGE RHIZOSPHERES. This study aimed to determine the diversity of Arbuscular Mycorrhizal Fungi (AMF) in several kinds of hybrid forages Rhizospheres, either in grasses or legumes. Soil samples were taken from three different locations, consisted of: Mapanget (forages type: Leucaena leucocepala, Sorghum varieties numbu, Penicettum purpureum cv. Mott), Tateli (forages type: calothyrsus Calliandra, Gliricidia sepium) and Campus of UNSRAT, Manado (forage type: King grass). The soil samples which taken from forages rhizospheres were sieved using Brundrett method and then centrifuged.  Thereafter, isolation and identification of spore were carried out based on spore morphology character, involves: shape, size, color, hyphae attachment, and ornament. Extraction and identification of spores on six types of rhizosphere were found 34 different types of AMF spores in shape and color. In grass, it was found three types of spores, i.e.: Glomus, Acaulospora, and Sclerocystis, meanwhile in leguminous just one type of spore was found, i.e.: Glomus. The difference of rhizosphere in grass and leguminosae resulted in different types of spores, where Sclerocystis and Acaulospora are found in grasses, on the contrary Sclerocystis and Acaulospora are not found in leguminosae. Based on the number of spores of AMF, it seemed that Glomus types found to have the most number, while Sclerocystis and Acaulospora had the lowest number of spores found. Key words: Exploration, Arbuscular Mycorrhizal Fungi (AMF), Grass, Legume, Spores

scholarly journals Acaulospora mellea and A. trappei, fungi new for Poland

2014 ◽  
Vol 34 (1) ◽  
pp. 41-50
Author(s):  
Janusz Błaszczykowski ◽  
Mariusz Tadych ◽  
Iwona Madej ◽  
Iwona Adamska ◽  
Beata Czerniawska ◽  
...  
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal ◽  
Soil Samples

Spores of <i>Acaulospora mellea</i> and <i>A. trappei</i> are described and illustrated. <i>Acaulospora mellea</i> occurs relatively infrequently in Poland. It was found in 30 of the more than 1300 soil samples examined representing different uncultivated and cultivated sites. Spores of <i>A. trappei</i> were only found in six field-collected soils. However, the occurrence of this fungus in many pot cultures with soils from various uncultivated and agricultural sites indicated that the species was common among arbuscular mycorrhizal fungi in Poland.

scholarly journals Morphological and Structural Diversities of Indigenous Mycorrhiza Communities Associated to Castor Bean from Adamawa Cameroon

2020 ◽  
pp. 16-24
Author(s):  
Lucien Tatchum Tchuenteu ◽  
Abba Maimouna ◽  
Olivia Wafo Youmbi ◽  
Clautilde Megueni
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Species Identification ◽  
Structural Characterization ◽  
Mycorrhizal Fungi ◽  
Castor Bean ◽  
Arbuscular Mycorrhizal ◽  
Soil Samples ◽  
Standard Methods ◽  
Subsaharan Africa ◽  
Scutellospora Calospora

This study describes the diversity of arbuscular mycorrhizal fungi (AMF) that enter into association with castor bean growth wild in Subsaharan Africa. Three sites of castor bean stands were selected in each of the three Subdivisions (Ngaoundere I, Ngaoundere II and Nyambaka) of the Vina Division in Adamawa Cameroon. Soil samples and roots were taken from each castor bean rhizosphere. All samples from one site were mixed into a composite sample. Leek was used as trap plant. Mycorrhizal parameters, spores density and specific richness were determined following to the standard methods. After spore extraction, species identification was obtained through the informations provided by the International Vesicular Mycorrhizal fungi collection. Results indicate that Cameroonian castor bean accession was found to be symbiotic with AMF under Sudano-Guinean climate of Adamawa Cameroon. The morphological and structural characterization enabled the description of six AMF species, belonging to three genera: Glomus fasciculatum, Glomus sp1, Glomus sp2, Scutellospora calospora,  Scutellospora purpurasens, Entrophospora infrequens. These findings open opportunities for domestication and application of AMF for a sustainable castor bean productivity.

scholarly journals Biocrusts inside and outside of Mimosa luisana resource islands as reservoirs of arbuscular mycorrhizal fungi in a Mexican semiarid ecosystem

2021 ◽  
Vol 51 ◽  
pp. e1370
Author(s):  
Sara Lucía Camargo-Ricalde ◽  
Noé Manuel Montaño ◽  
Susana A. Montaño-Arias ◽  
Claudia Janette De la Rosa-Mera ◽  
Eduardo Chimal-Sánchez
Keyword(s):  
Species Richness ◽  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Seasonal Changes ◽  
Fungal Spores ◽  
Arbuscular Mycorrhizal ◽  
Soil Samples ◽  
High Potential ◽  
Spore Abundance ◽  
Semiarid Ecosystem

Background: Arbuscular mycorrhizal fungi (AMF) and biocrusts (BC), occur inside and outside Mimosa luisana resource islands (M. luisana-RI) at the Tehuacán-Cuicatlán Valley, Puebla-Oaxaca, Mexico. Objectives: To determine: 1) Whether there are AMF within biocrusts, 2) The abundance and richness of AMF, and 3) The potential of AMF propagation in BC and soil below BC inside (BC-RI, soil-BC-RI) and outside (BC-ORI, soil-BC-ORI) M. luisana-RI, and open areas (OA), in the rainy (September 2011) and dry (May 2012) seasons. Methods: AMF were extracted of biocrusts and soil samples collected inside and outside M. luisana-RI and OA, in both seasons. Spore abundance and species richness, as well as potential propagation of AMF were determined in laboratory and greenhouse. Results and Conclusions: Biocrusts inside and outside M. luisana-RI form reservoirs of AMF spores and species richness (12 spp.), and act as “shields” protecting AMF compared with OA (5 spp.). Seasonal changes in the AMF composition within the biocrusts and the soil suggest that the availability of water drives AMF assemblages. The AM fungal spores in BC-RI and BC-ORI have a high potential of propagation; however, the BC-ORI by buffering the loss of AMF in soil-BC-ORI, they form mycorrhizal inocula within the soil.

scholarly journals Identification of Arbuscular Mychorizae Fungi on Oil Palm in Bireuen, Aceh

2021 ◽  
Vol 5 (2) ◽  
pp. 114-121
Author(s):  
Zaitun Ritaqwin Rita ◽  
Mizan Maulana ◽  
Nazalia
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Oil Palm ◽  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal ◽  
Culture Method ◽  
Soil Samples ◽  
Soil Biology ◽  
National University ◽  
Maize Plants ◽  
Very High

Abstract   Oil palm plantations in Aceh , especially in Bireuen, are generally planted on red-yellow podzolic soil types. Arbuscular mycorrhizal fungi (AMF) are fungi who are symbiotically associated with 97% of plant species. This study aims to identify the type and population of mycorrhizal spores from oil palm rhizosphere soil samples using culture trapping techniques (maize, sorghum, kudzu). This research was carried out in a greenhouse and Experimental Field, Faculty of Agriculture, Islamic National University of Indonesia, Bireuen, Aceh. Isolation, identification and observation of AMF colonization on plant roots was carried out at the Laboratory of Soil Biology, Faculty of Agriculture, Syiah Kuala University. The parameters included the number of arbuscular mycorrhizal fungi spores and the percentage of root colonization using the trapping culture method. The trapping culture method used 50 g of soil samples on oil palm rhizosphere which aged 5 months, 7 years, 9 years and 12 years. The results showed that the most dominant mycorrhizae found was the Glomus type. The mycorrhizae spores types found in the trapping culture were Glomus, Acaulospora and Gigaspora. The host plant that produced the most AMF spores was sorghum, while the highest AMF colonization of the three host plants was found in the roots of maize plants (78%) with very high criteria. Keywords: Oil palm, Culture trapping, AMF  

scholarly journals Diversity of Arbuscular Mycorrhizal Fungi of Durum Wheat (Triticum durum Desf.) Fields of the East of Algeria

2017 ◽  
Vol 9 (3) ◽  
pp. 117 ◽  
Author(s):  
Wassila Nadji ◽  
Nadir Belbekri ◽  
Nadia Ykhlef ◽  
Abdelhamid Djekoun
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Durum Wheat ◽  
Mycorrhizal Fungi ◽  
Triticum Durum ◽  
Chemical Characterization ◽  
Arbuscular Mycorrhizal ◽  
Soil Samples ◽  
Soil Conditions ◽  
Triticum Durum Desf ◽  
Semi Arid

In Algeria, in the semi-arid and arid areas cereal production is limited because of many constraints like lack of mineral elements (phosphorus and nitrogen), low organic matter and an insufficient water reserve due to the low rainfall. Most researches on durum wheat (Triticum durum Desf.) are undertaken to increase its productivity. In recent years several methods have been used including using microorganisms. Arbuscular Mycorrhizal fungi (AMF) appear to have important roles in plants in various soil conditions. In Algeria and particularly in the eastern regions, the diversity of AMF spores in the soil is so far little studied. In this context this study focuses on the study of the diversity and the abundance of AMF spores in Triticum durum Desf. fields in arid, semi-arid and coastal regions. Soil samples were collected and characterized and the spores isolated by the wet sieving methods were observed, counted and identified. The results of the physic-chemical characterization of all soil samples reveal a clay loam, loam clay and loam sandy soils with an alkaline pH (7.54-8.27), a total nitrogen content from 0.023 to 0.082% and phosphorus content ranging from 1.63% to 7.74%. We noted the presence of several morphotypes that were classified as follow: Glomeraceae, Acaulosporaceae and Scutellosporaceae with a predominance of Glomeraceae in different sites depending on climate and soil type. The presence of these families demonstrates a biodiversity of the eastern wheat fields of Algeria.

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