Quantitative Cell Count of <i>Vibrio vulnificus</i> Cells Based on MPN-PCR Method

2018 ◽  
Vol 33 (5) ◽  
pp. 412-415
Author(s):  
Yu-Mi Jang ◽  
Seul-Ki Park ◽  
Hee-Jin Jeong ◽  
Jang-Won Lee ◽  
Yohan Yoon ◽  
...  
Keyword(s):  
Cell Count ◽  
Vibrio Vulnificus ◽  
Pcr Method

scholarly journals Is the Rattus norvegicus Population Playing a Role in the Transmission of Zoonotic Diseases to Children? A Pilot Study in Tehran, Iran

2021 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Taher Azimi ◽  
Fatemeh Fallah ◽  
Mohammad Reza Pourmand ◽  
Abdollah Karimi ◽  
Shahnaz Armin ◽  
...  
Keyword(s):  
Rabies Virus ◽  
Vibrio Vulnificus ◽  
Urban Environments ◽  
Zoonotic Diseases ◽  
Control Programs ◽  
Leptospira Spp ◽  
Specific Igg ◽  
Pcr Method ◽  
One Year ◽  
Rodent Populations

Background: Due to frequent exposure to surface water and contact with animals, children represent a group susceptible to zoonotic diseases. Objectives: The present study aims to determine the presence and prevalence of the main zoonotic agents in R. norvegicus populations in Tehran, Iran. Methods: In the present study, 100 R. norvegicus were captured within a time span of one year from five districts of Tehran, Iran. Fecal and blood samples were collected from rodents and serum was recovered after centrifugation. The presence of specific IgG antibodies against Leptospira spp. and Rabies virus was detected using a commercial qualitative rat ELISA kit. A conventional PCR assay was employed to detect the presence of Vibrio vulnificus in the commensal R. norvegicus population. Results: In general, 80% (n = 80/100) and 20% (n = 20/100) of rats were males and females, respectively. The results of the ELSA assay showed that of the 100 R. norvegicus captured in Tehran, 7% (n = 7/100) and 1% (n = 1/100) were positive for Leptospira spp. and Rabies virus, respectively. Leptospira spp. revealed the highest frequency (20%; 4/20) among R. norvegicus collected from the eastern part of Tehran. Rabies virus was detected only from the southern (5%; 1/20) part of Tehran. Results of the PCR method showed that the percentage of the rats tested positive for V. vulnificus was 5%. Overall, the surveyed zoonotic microorganisms had the highest (n = 5/20; 25%) and lowest (n = 1/20; 5%) frequency rates in the eastern and northern parts of Tehran, respectively. Conclusions: The results accentuate the necessity of implementing rodent control programs and regular disinfection as well as avoiding contact with rodent populations in urban environments.

scholarly journals Simultaneous isolation and enumeration of virulent Vibrio cholerae and Vibrio vulnificus using an advanced MPN-PCR method

2021 ◽  
Vol 204 (1) ◽  
Author(s):  
Jae-Hwa Lee ◽  
Seul-Ki Park ◽  
Fazlurrahman Khan ◽  
Du-Min Jo ◽  
Do-ha Lee ◽  
...  
Keyword(s):  
Vibrio Cholerae ◽  
Vibrio Vulnificus ◽  
Pcr Method

scholarly journals Simultaneous Isolation and Enumeration of Virulent Vibrio Cholerae and Vibrio Vulnificus using an Advanced MPN-PCR Method

2021 ◽  
Author(s):  
Jae-Hwa Lee ◽  
Seul-Ki Park ◽  
Fazlurrahman Khan ◽  
Du-Min Jo ◽  
Do-ha Lee ◽  
...  
Keyword(s):  
Vibrio Cholerae ◽  
Vibrio Vulnificus ◽  
Detection Method ◽  
Low Cost ◽  
Luria Bertani ◽  
Most Probable Number ◽  
Probable Number ◽  
Pcr Method ◽  
Cell Enumeration ◽  
Differential Medium

Abstract Vibrio cholerae and Vibrio vulnificus are one of the critical foodborne pathogens that need to be intensively controlled their infection as a result of the intake and distribution of seafood, especially raw oysters. For this reason, various methods have already been developed for the detection and enumeration of these bacteria. The most probable number (MPN)-PCR (polymerase chain reaction) method is commonly used with the selective-differential medium for the efficiency and convenience of cell enumeration. One of the most frequently used for the detection of Vibrio spp. is Thiosulfate-Citrate-Bile salts-Sucrose (TCBS) agar. But this selective-differential medium can fail to distinguish between V. cholerae, V. vulnificus, and Vibrio alginolyticus. For this reason, the conventional MPN-PCR method with TCBS medium for the detection of Vibrio spp. has a problem that processing PCR to the two-times. This study suggests a simple and minimized detection method using one-time PCR and non-NaCl Luria-Bertani (LB-0) medium culture. This detection method is based on the difference in salt requirement between V. cholerae and V. vulnificus. Employing the developed methodology, the simultaneous cell enumeration of V. cholerae and V. vulnificus can be possible at a low cost. Furthermore, this study proposes a new specific primer to detect virulence-related genes from V. cholerae and V. vulnificus. This advanced MPN-PCR method was verified using bioaccumulated pacific oysters (Crassostrea gigas) by V. cholerae and V. vulnificus.

scholarly journals Identification of Vibrio vulnificus from Patients with Otitis Media and Septicemia by PCR Method

2001 ◽  
Vol 75 (4) ◽  
pp. 307-313 ◽  
Author(s):  
Susumu SATO ◽  
Tomisato MIURA ◽  
Masaaki SAITOH ◽  
Seishin TSUKIASHI ◽  
Toshiharu HONGO ◽  
...  
Keyword(s):  
Otitis Media ◽  
Vibrio Vulnificus ◽  
Pcr Method

scholarly journals Association of the <i>BoLA-DRB3</i> alleles with estimated breeding value for somatic cell count in Polish dairy cattle

2008 ◽  
Vol 51 (2) ◽  
pp. 111-119 ◽  
Author(s):  
G. Sender ◽  
K. G. A. Hameid ◽  
A. Korwin-Kossakowska ◽  
M. Sobczynska
Keyword(s):  
Dairy Cattle ◽  
Somatic Cell ◽  
Cell Count ◽  
Somatic Cell Count ◽  
Breeding Value ◽  
Holstein Cows ◽  
Breeding Programmes ◽  
Pcr Method ◽  
Estimated Breeding Value ◽  
Linear Contrast

Abstract. Introducing resistance to mastitis into breeding programmes for dairy cattle seems to be one of the possible methods for limiting the increasing number of mastitis cases. Looking for candidate genes for genetic markers is one of the strategies. The BoLA–DRB3 alleles have considerable promise as potential mastitis marker. The objective of the study was to evaluate relationships between two BoLA–DRB3 alleles (BoLA–DRB3.2*16 and BoLA-DRB3.2*23) and estimated breeding value (EBV) for somatic cell count (SCC) in Polish dairy cattle. A total of 525 Polish Holstein cows in two experimental farms were evaluated for test-day SCC. The polymorphism of BoLA–DRB3 gene was identified using the MPT-PCR method. The variance components for test-day SCC were estimated by the REML method using animal model. The breeding value of SCC was calculated according to the BLUP method. Linear contrast between model-adjusted breeding values of SCC for all animals was used to test for differences between genotypes. In Polish Holstein cows population BoLA allele DRB3.2*23 was associated with increase of EBV for SCC in milk. It was observed increase of EBV for SCC from cows carrying this allele comparing to cows carrying BoLA–DRB3.2*16 (p ≤0.07) or other alleles (p ≤0.01).

scholarly journals Method for the specific identification of the emerging zoonotic pathogen Vibrio vulnificus Lineage 3 (formerly Biotype 3)

2020 ◽  
Author(s):  
Hector Carmona-Salido ◽  
Naiel Bisharat ◽  
Carmen Amaro
Keyword(s):  
Vibrio Vulnificus ◽  
Genomic Analysis ◽  
Epidemiological Studies ◽  
Rapid Identification ◽  
Comparative Genomic ◽  
Zoonotic Pathogen ◽  
Genomic Study ◽  
Comparative Genomic Study ◽  
Pcr Method ◽  
Unique Ability

Vibrio vulnificus is a zoonotic pathogen that is spreading worldwide due to global warming. Lineage 3 (L3; formerly biotype 3) includes the strains of the species with the unique ability to cause fish farm-linked outbreaks of septicaemia. The L3-strains emerged recently and are particularly virulent and difficult to identify. Here we describe a new developed PCR method based on a comparative genomic study useful for both rapid identification and epidemiological studies of this interesting emerging group. The comparative genomic analysis also revealed the presence of a genetic duplication in the L3 strains that could be related to the unique ability of this lineage to produce septicemia outbreaks.

Evaluation of the Polymerase Chain Reaction Method for Identification of Vibrio vulnificus Isolated from Marine Environments

1997 ◽  
Vol 60 (1) ◽  
pp. 81-83 ◽  
Author(s):  
EIJI AONO ◽  
HARUO SUGITA ◽  
JUNJIRO KAWASAKI ◽  
HIROSHI SAKAKIBARA ◽  
TAKUMI TAKAHASHI ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Hybridization ◽  
Vibrio Vulnificus ◽  
Polymerase Chain Reaction Method ◽  
Tokyo Bay ◽  
Phenotypic Characteristics ◽  
Chain Reaction ◽  
Hemolysin Gene ◽  
Pcr Method ◽  
Polymerase Chain

The polymerase chain reaction (PCR) method for identification of Vibrio vulnificus in the marine environment was evaluated by comparing it to both the conventional and DNA-DNA hybridization methods. Of 13,325 isolates obtained from seawater and sediment samples, and oyster and goby specimens collected from the coastal waters of Tokyo Bay, Japan, only 61 isolates were identified as V. vulnificus on the basis of phenotypic characteristics and the amplification of the cytotoxin-hemolysin gene by the PCR method. All 61 isolates were further confirmed to be V.vulnificus by a DNA-DNA hybridization method and the API 20E system although they were divided into 13 groups on the basis of their API 20E profiles. These results strongly suggest that the PCR method is useful for identification of this organism.

Application of a novel pathogenicity marker in a multiplex real-time PCR method to assess total and pathogenic Vibrio vulnificus in food and environmental samples

Food Control ◽  
2014 ◽  
Vol 35 (1) ◽  
pp. 274-283 ◽  
Author(s):  
Alejandro Garrido-Maestu ◽  
María-José Chapela ◽  
Belén Román ◽  
Juan M. Vieites ◽  
Ana G. Cabado
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Environmental Samples ◽  
Vibrio Vulnificus ◽  
Pathogenic Vibrio ◽  
Pcr Method

Detection of vvha gene of Vibrio vulnificus and trh gene of Vibrio parahaemolyticus from Black Sea oysters using RT-PCR method

2017 ◽  
Vol 256 ◽  
pp. S68 ◽  
Author(s):  
Tudor Laurentiu ◽  
Mitranescu Elena ◽  
Simion Violeta ◽  
Pirvu Monica ◽  
Tudor Aneta Laura
Keyword(s):  
Black Sea ◽  
Vibrio Parahaemolyticus ◽  
Vibrio Vulnificus ◽  
Rt Pcr ◽  
Pcr Method
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