scholarly journals Effect of extracorporeal irradiation on segmental bone autograft incorporation in Sprague-Dawley rats

2014 ◽  
Vol 23 (3) ◽  
pp. 147-53 ◽  
Author(s):  
Muhammad Wahyudi ◽  
Achmad F. Kamal ◽  
Nurjati C. Siregar ◽  
Marcel Prasetyo
Keyword(s):  
En Bloc Resection ◽  
Bloc Resection ◽  
Control Group ◽  
Sprague Dawley ◽  
Negative Effects ◽  
En Bloc ◽  
Sprague Dawley Rats ◽  
Segmental Bone ◽  
Bone Autograft ◽  
Extracorporeal Irradiation

Background: Bone graft has been widely used in bone tumor reconstructive surgery. Extracorporeal irradiation (ECI) is commonly used to eliminate malignant cells before bone autograft. However, it may have negative effects on autograft incorporation. This study aimed to evaluate the ability of bone autograft incorporation after extra corporeal irradiation.Methods: 24 Sprague-Dawley rats underwent 7-mm en bloc resection of tibial diaphysis, and were divided into 4 groups. The first group did not receive irradiation; the 2nd, 3rd, and 4th groups received 50, 150 and 300 Gy bone irradiation respectively, and then reimplanted. Radiologic score were evaluated at week-6 and -8, while histopathology, osteoblast count and BMP-2 expression were examined at week-8. Data were analyzed with ANOVA or Kruskal-Wallis tests.Results: At week-6, radiologic scores in group 150 and 300 Gy were significantly lower compared to control group (4 vs 6 dan 4 vs 6; p = 0.011; p = 0.01). The same results were also obtained at week-8 (5.40 vs 7.14; p = 0.009 in the group 150 Gy and 5.60 vs 7.14; p = 0.018 in the group 300 Gy. Histopathological scores of the groups receiving 50, 150 and 300 Gy were significantly lower compared to the control group (6 vs 7, p = 0.017; 4 vs 7, p = 0.005; 6 vs 7, p = 0.013). Osteoblast count and BMP-2 expression were not significantly different among all groups.Conclusion: ECI with the dose of 50 to 300 Gy is associated with delayed bone autograft incorporation. However, the osteoinductive and osteogenesis capacity for autograft incorporation were maintained.

scholarly journals High proficiency of colonic endoscopic submucosal dissection in Europe thanks to countertraction strategy using a double clip and rubber band

2019 ◽  
Vol 07 (09) ◽  
pp. E1166-E1174 ◽  
Author(s):  
Jérémie Jacques ◽  
Aurélie Charissoux ◽  
Pierre Bordillon ◽  
Romain Legros ◽  
Jérôme Rivory ◽  
...  
Keyword(s):  
Case Series ◽  
Rubber Band ◽  
En Bloc Resection ◽  
Bloc Resection ◽  
Point Of View ◽  
University Hospital ◽  
Control Group ◽  
R0 Resection ◽  
Resection Rate ◽  
En Bloc

Abstract Background and study aims ESD in the colon is more challenging technically than in other locations. Here, we report the first comparative case series of colon ESD using a systematic countertraction strategy using two clips and a rubber band. Patients and methods Retrospective comparative study of classic versus countertraction colon ESD performed in colon ESD cases collected prospectively at Lyon Edouard Herriot Hospital and Limoges University Hospital from January 2016 until December 2017. Results The study included 192 cases (control = 76, countertraction = 116). Countertraction using the double clip and rubber band technique versus the control group resulted in a significant decrease in the procedure time (94.7 vs. 117 min; P = 0.004) and significant increases in procedure speed (28.2 vs. 16.7 mm2/min; P < 0.0001), en bloc resection rate (95.7 % vs. 76.3 %, P < 0.0001), and R0 resection rate (78.5 % vs. 64.5 %, P = 0.04).At an individual operator point of view, results varied between operators but the double clip countertraction strategy significantly increased the en bloc resection rate, R0 resection rate, and speed of dissection for each of the 4 operators. Conclusion Systematic countertraction using a double clip and rubber band facilitates colon ESD. This strategy should become the standard for colon ESD.

Sustained Consumption of an Infusion of Chaya Leaf (Cnidoscolus Aconitifolius) Does Not Affect Nutritional Biomarkers in Sprague Dawley Rats

2019 ◽  
Vol 18 (4) ◽  
pp. 373-377
Author(s):  
Kuri-García Aarón ◽  
Aranda-Vargas Paola Jazmín ◽  
Moreno-Celis Ulisses ◽  
Cervantes-Jiménez Ricardo ◽  
Mejía Carmen ◽  
...  
Keyword(s):  
Bodyweight Gain ◽  
Blood Analysis ◽  
Control Group ◽  
Sprague Dawley ◽  
Negative Effects ◽  
Daily Consumption ◽  
Beverage Consumption ◽  
Cnidoscolus Aconitifolius ◽  
Sprague Dawley Rats ◽  
Food And Beverage

Currently, the use of medicinal plants is one of the most important biotechnological alternatives in the treatment of numerous diseases, particularly non-communicable diseases. One such plant, Cnidoscolus aconitifolius, also known as “chaya” in Mexico, is a rich source of bioactive phytochemicals. It is widely distributed across Central America and has ornamental, food, and medicinal values. Studies are needed to confirm that there are no negative effects after sustained consumption of Cnidoscolus aconitifolius. Experiments using animal models can be used to evaluate the possible chemopreventive effects of this plant against various diseases consistent with ethnobotanical information. Accordingly, a preclinical longitudinal experimental study in Sprague Dawley rats was conducted at 16 and 32 weeks' after the daily consumption of an infusion of chaya leaf. Bodyweight gain, food and beverage consumption, blood analysis, and histological analysis of the organs responsible for metabolism and excretion were evaluated. No significant differences were found between the control group and the group that consumed the chaya leaf infusion after 16 and 32 weeks. The proposed experimental model may be reliably used for research purposes to evaluate the possible effectiveness of this plant in preventing and/or treating chronic diseases.

scholarly journals Saving a Child’s Elbow Joint: A Novel Reconstruction for a Tumour of the Distal Humerus

2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Calogero Graci ◽  
Czar Louie Gaston ◽  
Robert Grimer ◽  
Lee Jeys ◽  
Korhan Ozkan
Keyword(s):  
Distal Humerus ◽  
En Bloc Resection ◽  
Bloc Resection ◽  
Wide Resection ◽  
Parosteal Osteosarcoma ◽  
En Bloc ◽  
Fibular Autograft ◽  
Excellent Functional Result ◽  
Extracorporeal Irradiation ◽  
Inexpensive Technique

Reconstruction after wide resection of a malignant bone tumor can be obtained using several techniques such as the use of prostheses, allograft, autograft, or combined procedure. We describe a 12-year-old girl with parosteal osteosarcoma of the distal right humerus treated by en bloc resection, intraoperative extracorporeal irradiation, and implantation. We inserted a nonvascularised fibular autograft through the middle of irradiated graft to obtain a greater stability. We have not recorded any complication associated with this technique such as nonunion, pathological fracture, infection, and bone necrosis and we obtained an excellent functional result. 10 years after surgery, the patient had no recurrence. Extracorporeal irradiation and reimplantation is a valid and inexpensive technique for the treatment of bone tumors when there is reasonable residual bone stock. With this procedure we have a precise fit being the patient’s own bone. In this way we avoid all the problems related to the adaptation of the shape and size.

scholarly journals Effect of autoclave devitalization on autograft incorporation and bone morphogenetic protein of tibia in Sprague-Dawley rats

2014 ◽  
Vol 23 (2) ◽  
pp. 74-8 ◽  
Author(s):  
Anak A.G.Y. Asmara ◽  
Achmad F. Kamal ◽  
Nurjati C. Siregar ◽  
Marcel Prasetyo
Keyword(s):  
Bone Morphogenetic Protein ◽  
Limb Salvage ◽  
Control Group ◽  
Heating Process ◽  
Limb Salvage Surgery ◽  
Sprague Dawley ◽  
En Bloc ◽  
Morphogenetic Protein ◽  
Sprague Dawley Rats ◽  
Radiological Score

Background: Heating process with autoclave is one of limb salvage modalities that are widely used. but the results are not satisfying, due to mechanical bone fragility. However, considering this treatment modality is widely accepted in terms of financial, religion and sociocultural aspects, we conducted a on study rats treated with resection and reconstruction with autoclave heating method to assess bone healing by sequential radiology, histopathologic osteoblasts count, and bone morphogenetic protein (BMP).Methods: Thirty six Sprague-Dawley rats were divided into two groups with one group being the autoclave group and others served as control group. In both groups, the tibial diaphysis was extracted en bloc for 7 mm. All groups were kept for 8 weeks and treated under the same condition except the autoclave group, where the extracted bones were put into autoclave at 134°C for 15 minutes and refixed again with k-wire. We performed radiological examination at 5th and 8th week using Lane and Sandhu radiological score. After extraction, the tibial bones were inspected for histological pattern using Salked modified score, osteoblast quantity counting and BMP-2 values.Results: There were statistically significant diffences between control and autoclave group on radiological score at 5th (5.12 ± 1.6 g vs 3.21 ± 2.42, p = 0.023) and 8th week (6.06 ± 1.71 vs 4.29 ± 2.53, p = 0.040), histological score between groups (6.06 ± 1.14 vs 4.14 ± 1.99, p = 0.005), osteoblast count (p < 0.001), and BMP-2 expression,  respectively.Conclusion: Autoclave recycling autograft lowered the speed of graft incorporation and BMP-2 expression. Therefore, autoclave recycling autograft as a method of limb salvage surgery must be reevaluated and not considered to be applied for treatment in bone malignancy.  

En bloc resection, extracorporeal irradiation, and reimplantation of an entire tibia

2006 ◽  
Vol 11 (3) ◽  
pp. 298-302 ◽  
Author(s):  
Shinobu Takahashi ◽  
Shuzo Okudaira ◽  
Keisuke Sasai ◽  
Yoshihiko Kotoura
Keyword(s):  
En Bloc Resection ◽  
Bloc Resection ◽  
En Bloc ◽  
Extracorporeal Irradiation

scholarly journals SURG-04. THE APPLICATION OF EN BLOC RESECTION IN THE OPERATION OF PEDIATRIC POSTERIOR FOSSA TUMOR

2020 ◽  
Vol 22 (Supplement_3) ◽  
pp. iii461-iii461
Author(s):  
Ma Jie ◽  
Weiwei Mao ◽  
Shuaiwei Tian ◽  
Baocheng Wang ◽  
Yang Zhao
Keyword(s):  
Posterior Fossa ◽  
Tumor Resection ◽  
Operation Time ◽  
En Bloc Resection ◽  
Bloc Resection ◽  
Posterior Fossa Tumor ◽  
Control Group ◽  
Observation Group ◽  
Resection Rate ◽  
En Bloc

Abstract OBJECTIVE To explore the efficacy and safety of en bloc resection therapy on posterior fossa tumor in children. METHODS A retrospective analysis was conducted on the clinical data of 94 patients with posterior fossa tumor admitted to Department of Pediatric Neurosurgery, Xinhua Hospital Affiliated to Shanghai Jiaotong University School Of Medicine from January 2018 to December 2019. Among them, 35 cases were treated with traditional resection (control group) and 59 cases with en bloc resection (observation group). We counted the amount of blood loss and the time during tumor resection, We compare the symptoms and signs between the two groups and determine a extent of tumor resection based on microscopic observation and preoperative and postoperative imaging comparison. RESULT: The total tumor resection rate of the observation group (88.1%, 52 / 59) was significantly higher than that of the control group (62.85%, 22 / 35, P &lt; 0.05). The average bleeding volume of 90.8ml in the observation group was significantly smaller than that of the control group (113.3ml, P &lt; 0.05), and the average operation time of 38.6min in the observation group was shorter than that of the control group (57.4min, P &lt; 0.05) only for tumor resection procedure. CONCLUSION En bloc resection technique can effectively accelerate the resection time, reduce intraoperative bleeding and improve the total resection rate of tumors in children’s posterior cranial fossa.

scholarly journals Gastric ESD under Heparin Replacement at High-Risk Patients of Thromboembolism Is Technically Feasible but Has a High Risk of Delayed Bleeding: Osaka University ESD Study Group

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Toshiyuki Yoshio ◽  
Tsutomu Nishida ◽  
Naoki Kawai ◽  
Kiyonori Yuguchi ◽  
Takuya Yamada ◽  
...  
Keyword(s):  
High Risk ◽  
Antithrombotic Therapy ◽  
Perioperative Period ◽  
En Bloc Resection ◽  
Bloc Resection ◽  
Gastric Neoplasms ◽  
Control Group ◽  
En Bloc ◽  
Bleeding Rate ◽  
Delayed Bleeding

Objectives. Heparin replacement (HR) is often performed in patients with a high risk of thrombosis undergoing endoscopic procedures. However, information about the influence of HR is scarce. The aim of this study is to assess the clinical impact of HR for gastric endoscopic submucosal dissection (ESD).Methods. This is a retrospective study comprising approximately 1310 consecutive gastric neoplasms in 1250 patients, who underwent ESD in 5 institutes. We assessed the clinical findings and outcomes of ESD under HR, compared to ESD without HR as control.Results. A total of 24 EGC lesions in 24 patients were treated by ESD under HR. In the HR group, the complete en-bloc resection rate was 100%. The delayed bleeding rate was, however, higher in the HR group than in the controls (38% versus 4.6%). The timing of bleeding in the HR group was significantly later than in controls. In the control group, 209 patients discontinued antithrombotic therapy during perioperative period, and their delayed bleeding rate was not different from those without antithrombotic therapy (5.7% versus. 4.4%). A thromboembolic event was encountered in 1 patient under HR after delayed bleeding.Conclusion. ESD under HR is technically feasible but has a high risk of delayed bleeding.

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