Von Kossa and his staining technique

One hundred and twenty years ago, the Hungarian physician Julius von Kossa developed a now classical staining method for detecting mineral deposits in animal tissues. Since then, this method has been widely adapted and combined with different counterstains, but still bears the name of its original inventor, who, if alive, would have turned 150 in 2015. As a rather inexpensive technique that does not require special instrumentation, von Kossa’s staining method became extremely popular for demonstrating mineralized tissues in vivo and in vitro. This article pays tribute to von Kossa and to his handy staining method.

Algorithm of management based on treatment goals for calcifying tendonitis of the shoulder: rigidity, pain and size of calcification

Purpose: The objective of the present manuscript is to propose a step-by-step algorithm for the management of calcifying tendonitis (CT) of the shoulder based on treatment goals, from conservative to surgical approaches. Method: A clinical note to present the main treatments for the management of calcifying tendonitis of the shoulder based on pain, rigidity and size of calcification based on the clinical experience and previous publications of the authors has been performed. Arguments: Treatment is conservative and surgical although there is controversy on the most adequate treatment. Kinesiotherapy is the recommended therapy for shoulder rigidity. For pain management after NSAIDs; microwaves, short waves, TENS, ultrasounds and Interferentials are effective. For the management of size of calcification, Iontophoresis, Electroshock wave therapy, Ultrasound needle guided aspiration and arthroscopic surgeries are the recommended alternatives, in that order. Conclusions: CT of the shoulder must be treated based on specific goals, mainly pain, rigidity or size of calcification. The proposed step-by-step algorithm of treatment is suggested based on the effectiveness of available techniques. If rigidity is present, kinesiotherapy is the recommended technique. For pain management, physical therapy such as microwaves, short waves, TENS, Ultrasound and Interferentials are effective techniques. For the treatment of calcification, iontophoresis is the most common, safe and inexpensive technique. If all previous conservative techniques failed, advanced techniques such as ESWT, US guided aspiration and arthroscopy are recommended, although they are not exempt of risk factors and complications.

C-reactive Protein (CRP) as Diagnostic Marker of Pneumonia in Children

Pneumonia is an inflammation of the lung parenchyma tissue that mainly affects the respiratory bronchioles and alveoli. The present research study is focused on assessment of the C-reactive protein (CRP) test as a tool marker for the diagnosis of pneumonia among children. In the current study, a total of 450 patients admitted to the children ward at Lady Reading Hospital (LRH) Peshawar, Khyber Pakhtunkhwa, Pakistan were selected, out of which 55% were male and 45% were female. In addition, two groups were designed according to age i.e. 1-8 years and 9-16 years of age with a frequency of 55 and 45%, respectively. In case of chest X-ray assessment, it was observed that all patients were highly susceptible to pneumonia infection, while from blood culture analysis, it was perceived that 419 patients (93%) were highly susceptible to pneumonia infection. Two bacterial isolates (Klebsiella pneumoniae and Streptococcus pneumoniae) were characterised from all positive samples. In contrast with CRP assessment, 385 patients (85%) had positive CRP reports displaying more than 5 mg/L CRP values and 65 patients (15%) had negative CRP reports. Thus, it was concluded that patients with high CRP values presented clear evidence of a severe infection. Moreover, it is suggested that CRP is a sensitive, less time consuming and inexpensive technique; therefore, it has been recommended to be used as a tool marker along with other diagnostic techniques for the diagnosis of life-threaten infections to get a more clear picture about infections.

Genetic Diversity and Population Structure of Algerian Endemic Plant Species Avena macrostachya Bal. ex Cross. et Durieu

Avena macrostachya is still a relatively unknown species. Using sequence-related amplified polymorphism (SRAP) markers, a simple and inexpensive technique, allowed us to conduct experiments on genetic differentiation and to study the population structure of this Algerian endemic oat. The results obtained showed lower than expected genetic diversity within the A. macrostachya species. The cause may be endemism of the species as well as genetic drift possible during collection, and maintenance of the accessions in gene bank and seed reproduction. No clear genetic structure was found in the examined collection, which indicates a close relationship between the populations collected in the Djurdjura National Park in Algeria. Considering the endemism of the species, its breeding potential and the small-scale ex situ collection, careful monitoring of natural sites and repeating of the collection mission are, therefore, absolutely crucial.

Advantages of Using UAV Digital Photogrammetry in the Study of Slow-Moving Coastal Landslides

The aim of this study is to demonstrate the advantages of using micro drones in the study of large slow-moving landslides, which are widespread along the northwestern coast of Malta. In particular, attention was given to the inventory and analysis of gravity-induced joints and megaclast deposits at four study sites selected due to the presence of remarkable examples of lateral spreads evolving into block slides. The research was carried out by means of Google Earth (GE) image analysis and uncrewed aerial vehicle digital photogrammetry (UAV-DP). UAV-DP outputs enabled the identification and characterization of tens of persistent joints (locally exceeding 150 m) and permitted the size categorization of thousands of blocks. With reference to gravity-induced joints, a favorable agreement was found between existing datasets (mainly based on the integration of GE analysis and field survey) and UAV-DP outputs in terms of the identification of joints and their persistence. Conversely, the use of the UAV-DP technique showed significant advantages in terms of joint aperture determination (even exceeding 1 m) and distribution setting. Regarding the extensive megaclast deposits, UAV-DP enabled the identification of 8943 individuals which, compared with the 5059 individuals identified by GE analysis, showed an increase in the total population of 76%. This is related to the high accuracy of DP-derived orthomosaics and 3D models, which are particularly useful for identifying detached blocks. The inexpensive technique used in this research highlights its potential for being extended to other rocky coastal areas affected by slow-moving landslides.

Laser writing of nitrogen-doped silicon carbide for biological modulation

Conducting or semiconducting materials embedded in insulating polymeric substrates can be useful in biointerface applications; however, attainment of this composite configuration by direct chemical processes is challenging. Laser-assisted synthesis has evolved as a fast and inexpensive technique to prepare various materials, but its utility in the construction of biophysical tools or biomedical devices is less explored. Here, we use laser writing to convert portions of polydimethylsiloxane (PDMS) into nitrogen-doped cubic silicon carbide (3C-SiC). The dense 3C-SiC surface layer is connected to the PDMS matrix via a spongy graphite layer, facilitating electrochemical and photoelectrochemical activity. We demonstrate the fabrication of arbitrary two-dimensional (2D) SiC-based patterns in PDMS and freestanding 3D constructs. To establish the functionality of the laser-produced composite, we apply it as flexible electrodes for pacing isolated hearts and as photoelectrodes for local peroxide delivery to smooth muscle sheets.

Biosensor of inflammation biomarkers based on electrical bioimpedance analysis on immobilized DNA without chemical modification

The development of biosensors to identify molecular markers or specific genes is fundamental for the implementation of new techniques that allow the detection of specific Deoxyribonucleic acid (DNA) sequences in a fast, economic and simple way. Different detection techniques have been proposed in the development of biosensors. Electrical Bioimpedance Spectroscopy (EBiS) has been used for diagnosis and monitoring of human pathologies, and is recognized as a safe, fast, reusable, easy and inexpensive technique. This study proves the development of a complementary DNA (cDNA) biosensor based on measurements of EBiS and DNA's immobilization with no chemical modifications. The evaluation of its potential utility in the detection of the gene expression of three inflammation characteristic biomarkers (NLRP3, IL-1β and Caspase 1) is presented. The obtained results demonstrate that EBiS can be used to identify different gene expression patterns, measurements that were validated by Quantitative Polymerase Chain Reaction (qPCR). These results indicate the technical feasibility for a biosensor of specific genes through bioimpedance measurements on the immobilization of cDNA.