scholarly journals Role of nuclear factor kappa beta, tumor necrosis factor α, and cyclooxygenase-2 in preterm labor

2015 ◽  
Vol 23 (4) ◽  
pp. 213-7
Author(s):  
Syamsul B. Riva'i ◽  
Eryati Darwin ◽  
Ellyza Nasrul ◽  
Jusuf S. Effendi
Keyword(s):  
Tumor Necrosis Factor ◽  
Preterm Labor ◽  
Tumor Necrosis ◽  
Cyclooxygenase 2 ◽  
Nuclear Factor ◽  
Nuclear Factor Kappa ◽  
Nuclear Factor Kappa Beta ◽  
Cox 2 ◽  
Term Labor ◽  
Necrosis Factor

Background: The pathway of tumor necrosis factor alpha (TNFα), nuclear factor kappa beta (NF-κB), and cyclooxygenase-2 (COX-2) activation in releasing prostaglandins is suggested to be crucial for initiating labor in the pathogenesis of preterm labor. The aim of the study was to know whether there were mean differences of NF-κB, TNFα, and COX-2 expressions between preterm and term labor and also to know the correlation among them in preterm labor.Methods: A case-control study was performed from May 2013 to February 2014 in Arifin Achmad Hospital, Pekanbaru. There were 30 subjects with preterm labor as cases and 30 with normal labor as controls. All subjects had singleton gestation with maximum parity was three, age limit of 35 year-old, and spontaneous labor in both groups. Placental tissue was collected from all subjects and evaluated with hematoxylin eosin staining. The expressions of TNFα, NF-κB, and COX-2 in the tissue were assessed with immunohistochemical staining by counting the percentage of smeared cells by two experts. The expressions of TNFα, NF-κB, and COX-2 between case and control were compared using t-test and the correlation was analyzed with Pearson correlation coefficient.Results: Mean (SD) of expressions of TNFα (93.05% [12.68] vs 49.11% [27.33]), NF-κB (42.46% [27.29] vs 13.66% [17.77]), and COX-2 (88.75% [10.86] vs 46% [30.36]) were significantly higher in the preterm labor compared to term labor (p = 0.001). There was significant correlation between TNFα and NF-κB expression (r = 0.385; p = 0.036) and no correlation was found between NF-κB and COX-2 (p = 0.982) in preterm labor.Conclusion: High expressions of TNFα, NF-κB, and COX-2 in preterm labor showed to contribute in the onset of preterm labor. High TNFα may suggest that infection was a leading cause of preterm labor. This is supported with an increase in NF-κB activation will increase COX-2 and subsequently prostaglandins that result in premature labor.

Effect of a nonprotein bioactive agent on the reduction of cyclooxygenase-2 and tumor necrosis factor–α in human intervertebral disc cells in vitro

2008 ◽  
Vol 9 (5) ◽  
pp. 411-418 ◽  
Author(s):  
Tatsuhiro Yoshida ◽  
Jin Soo Park ◽  
Kimiaki Yokosuka ◽  
Kotaro Jimbo ◽  
Kei Yamada ◽  
...  
Keyword(s):  
Tumor Necrosis Factor ◽  
Intervertebral Disc ◽  
Tumor Necrosis ◽  
Cyclooxygenase 2 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Mrna Levels ◽  
Disc Cells ◽  
Cox 2 ◽  
Antiinflammatory Effects ◽  
Necrosis Factor

Object Neurotropin is a nonprotein extract from the inflamed skin of rabbits inoculated with vaccinia virus. In the present study the authors sought to clarify the focal antiinflammatory effects of Neurotropin in intervertebral disc cells, and these effects were compared with those induced by the selective cyclooxygenase (COX)–2 inhibitor 6-methoxy-2-naphthylacetic acid (nabumetone). Methods Six human intervertebral disc specimens were harvested during spinal surgery for lumbar disc herniation. Cells were stimulated with 500 pg/ml of interleukin (IL)–1β in the presence of various concentrations of Neurotropin (0, 10−5, 10−4, and 10−3 Neurotropin Units/ml) or 50 μg/ml of nabumetone for 3 hours. The mRNA was extracted for polymerase chain reaction (PCR), and real-time PCR was used to quantify the mRNA levels of COX- 2, tumor necrosis factor (TNF)–α, and phospholipase A2. Cyclooxygenase-2, TNFα, and prostaglandin E2 (PGE2) protein concentrations were each determined by enzyme-linked immunosorbent assay. Results Neurotropin was found to significantly suppress the expression of COX-2 and TNFα at mRNA levels as well as the concentration of COX-2 at protein levels in a dose-dependent manner. Nabumetone was found to significantly increase COX-2 at mRNA levels but directly suppress the concentration of PGE2 in culture medium. Conclusions Results in this study suggest that Neurotropin has an analgesic effect through the suppression of COX-2 and TNFα in a focal area, and nabumetone shows this same effect through the suppression of PGE2 production. Thus, Neurotropin could decrease pain by blocking the central pain pathway or increasing focal antiinflammatory effects.

scholarly journals Tumor necrosis factor alpha-mediated inhibition of melanogenesis is dependent on nuclear factor kappa B activation

Oncogene ◽  
1999 ◽  
Vol 18 (8) ◽  
pp. 1553-1559 ◽  
Author(s):  
Walter Englaro ◽  
Philippe Bahadoran ◽  
Corine Bertolotto ◽  
Roser Buscà ◽  
Benoit Dérijard ◽  
...  
Keyword(s):  
Tumor Necrosis Factor ◽  
Tumor Necrosis Factor Alpha ◽  
Tumor Necrosis ◽  
Nuclear Factor Kappa B ◽  
Nuclear Factor ◽  
Necrosis Factor Alpha ◽  
Nuclear Factor Kappa ◽  
Factor Alpha ◽  
Necrosis Factor
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