The Effects of Cytokines in Adipose Stem Cell-Conditioned Medium on the Migration and Proliferation of Skin FibroblastsIn Vitro

Although adipose stem cell-conditioned medium (ASC-CM) has demonstrated the effect of promoting the cutaneous wound healing, the mechanism for this response on the effector cells (e.g., dermal fibroblasts) during the process remains to be determined. In this study, we aim to investigate the types and contents of cytokines in ASC-CM and the effects of some kinds of common cytokines in ASC-CM, such as EGF, PDGF-AA, VEGF, and bFGF, on dermal fibroblasts proliferation and migration in wound healing process. Results showed that these four cytokines had high concentrations in ASC-CM. The migration of skin fibroblasts could be significantly stimulated by VEGF, bFGF, and PDGF-AA, and the proliferation could be significantly stimulated by bFGF and EGF in ASC-CM. Additionally, ASC-CM had more obvious promoting effect on fibroblasts proliferation and migration than single cytokine. These observations suggested that ASC-CM played an important role in the cutaneous injury partly by the synergistic actions of several cytokines in promoting dermal fibroblasts proliferation and migration, and ASC-CM was more adaptive than each single cytokine to be applied in promoting the wound healing.

Download Full-text

Wound Healing Promotion by Hyaluronic Acid: Effect of Molecular Weight on Gene Expression and In Vivo Wound Closure

Pharmaceuticals ◽

10.3390/ph14040301 ◽

2021 ◽

Vol 14 (4) ◽

pp. 301

Author(s):

Yayoi Kawano ◽

Viorica Patrulea ◽

Emmanuelle Sublet ◽

Gerrit Borchard ◽

Takuya Iyoda ◽

...

Keyword(s):

Gene Expression ◽

Molecular Weight ◽

Wound Healing ◽

Hyaluronic Acid ◽

Healing Process ◽

Dermal Fibroblasts ◽

Water Soluble ◽

Wound Healing Process ◽

And Migration ◽

Proliferation And Migration

Hyaluronic acid (HA) has been known to play an important role in wound healing process. However, the effect of molecular weight (MW) of exogenously administered HA on the wound healing process has not been fully understood. In this study, we investigated HA with different MWs on wound healing process using human epidermal keratinocytes and dermal fibroblasts. Cell proliferation and migration ability were assessed by water soluble tetrazolium (WST) assay and wound scratch assay. We examined the effect of HA addition in a full-thickness wound model in mice and the gene expression related to wound healing. Proliferation and migration of HaCaT cells increased with the increase of MW and concentration of HA. Interleukin (IL-1β), IL-8 and vascular endothelial growth factor (VEGF) as well as matrix metalloproteinase (MMP)-9 and MMP-13 were significantly upregulated by high molecular weight (HMW) HA in keratinocytes. Together with VEGF upregulation and the observed promotion of HaCaT migration, HA with the MW of 2290 kDa may hold potential to improve re-epithelialization, a critical obstacle to heal chronic wounds.

Download Full-text

Promotive effects of human induced pluripotent stem cell-conditioned medium on the proliferation and migration of dermal fibroblasts

Biotechnology and Bioprocess Engineering ◽

10.1007/s12257-017-0221-1 ◽

2017 ◽

Vol 22 (5) ◽

pp. 561-568 ◽

Cited By ~ 9

Author(s):

Myeongsik Oh ◽

Yu Jin Kim ◽

Young Ju Son ◽

Hyuk Sang Yoo ◽

Ju Hyun Park

Keyword(s):

Stem Cell ◽

Conditioned Medium ◽

Pluripotent Stem Cell ◽

Induced Pluripotent Stem Cell ◽

Dermal Fibroblasts ◽

And Migration ◽

Induced Pluripotent ◽

Cell Conditioned Medium ◽

Proliferation And Migration

Download Full-text

Conjugation of CTGF with Reduced Graphene Oxide Nanoparticles for the Development of Wound Healing Hydrogel Patch

University of the Future: Re-Imagining Research and Higher Education ◽

10.29117/quarfe.2020.0180 ◽

2020 ◽

Author(s):

Raza ur Rehman Syed ◽

Robin augustine ◽

Alap ali Zahid ◽

Anwarul Hasan

Keyword(s):

Wound Healing ◽

Graphene Oxide ◽

Reduced Graphene Oxide ◽

Chronic Wounds ◽

Healing Process ◽

Wound Healing Process ◽

Reduced Graphene ◽

And Migration ◽

Migration Of Cells ◽

Proliferation And Migration

Non-healing chronic wounds are the key concern in type-2 diabetes that frequently leads to chronic infections, finally causing amputation of limbs, organs etc. Decrease in the proliferation and migration of cells such as keratinocytes and fibroblasts is the major reason for the development of such chronic diabetic wounds. Multiple evidences have shown that CTGF and reduced graphene oxide possesses angiogenic property and promote wound healing by promoting proliferation and migration of fibroblasts and keratinocytes cells.Conjugation of rGO with CTGF using EDC-NHS chemistry is a novel approach to accelerate the wound healing process. In the current work, we have developed a rGO/CTGF incorporated GelMA hydrogel dressing to improve wound healing by increasing proliferation and migration of cells as well as promoting formation of new blood vessels for increased supply of nutrients, oxygen and growth factors to wound area

Download Full-text

Puerarin Improves Dexamethasone-Impaired Wound Healing In Vitro and In Vivo by Enhancing Keratinocyte Proliferation and Migration

Applied Sciences ◽

10.3390/app11199343 ◽

2021 ◽

Vol 11 (19) ◽

pp. 9343

Author(s):

Ly Thi Huong Nguyen ◽

Sang-Hyun Ahn ◽

Min-Jin Choi ◽

In-Jun Yang ◽

Heung-Mook Shin

Keyword(s):

Wound Healing ◽

Healing Process ◽

Wound Healing Process ◽

Hacat Cells ◽

Impaired Wound Healing ◽

Keratinocyte Proliferation ◽

And Migration ◽

Proliferation And Migration

The delayed and impaired wound healing caused by dexamethasone (DEX) is commonly reported. Puerarin, the major isoflavone found in Pueraria montana var. lobata (Willd.) Sanjappa & Pradeep promoted the wound healing process in diabetic rats. However, the effects and underlying mechanisms of puerarin on DEX-impaired wound healing have not been investigated. This study examined the potential uses of puerarin in upregulating keratinocyte proliferation and migration in dexamethasone (DEX)-suppressed wound healing model. The effects of puerarin on wound healing in vivo were investigated by taking full-thickness 5 mm punch biopsies from the dorsal skin of BALB/c mice and then treating them topically with 0.1% DEX. For the in vitro study, DEX-treated HaCaT cells were used to examine the effects of puerarin on DEX-induced keratinocyte proliferation and migration and the mechanisms of its action. Puerarin, when applied topically, accelerated the wound closure rate, increased the density of the capillaries, and upregulated the level of collagen fibers and TGF-β in the wound sites compared to the DEX-treated mice. Puerarin promoted the proliferation and migration of keratinocytes by activating the ERK and Akt signaling pathways in DEX-treated HaCaT cells. In conclusion, puerarin could be effective in reversing delayed and disrupted wound healing associated with DEX treatments.

Download Full-text

Adipose derived stem cell conditioned medium effect on proliferation phase of wound healing in Sprague Dawley rat

Medical Journal of Indonesia ◽

10.13181/mji.v26i4.1755 ◽

2018 ◽

Vol 26 (4) ◽

pp. 239-45 ◽

Cited By ~ 2

Author(s):

Twidy Tarcisia ◽

Lia Damayanti ◽

Radiana D. Antarianto ◽

Yefta Moenadjat ◽

Jeanne A. Pawitan

Keyword(s):

Wound Healing ◽

Stem Cell ◽

Conditioned Medium ◽

Healing Process ◽

Basal Medium ◽

Medium Effect ◽

Sprague Dawley ◽

Collagen Density ◽

Adipose Derived Stem Cell ◽

Cell Conditioned Medium

Background: Disintegration of skin tissue can lead to disability and death. Recent studies on wound therapy applied stem cells and adipose derived stem cell conditioned medium (ADSC-CM) to improve wound healing. However, the role of ADSC-CM in wound healing mechanism in terms of angiogenesis, quantity of collagen, and epithelialization is not fully understood. Therefore, this study aimed to analyze the levels of growth factors (VEGF and EGF) in ADSC-CM and histological features of angiogenesis, epithelialization, and collagen density after skin incision in Sprague Dawley rats.Methods: Thirty rats were injured at the back (full thickness wound) and treated topically with ADSC-CM, culture medium, basal medium, and without treatment. Mice were sacrificed on days 3, 7, 14, 21 and 28. After sacrificed, tissue samples were examined microscopically to assess angiogenesis, epithelialization, and collagen density. Concentrations of VEGF and EGF in ADSC-CM were measured by ELISA.Results: Clinically, wound that was treated with ADSC-CM showed improvement in wound healing process. ADSC-CM treated wound showed the highest epithelialization ratio and the fastest wound closure.Conclusion: There were no statistical significant differences between groups that were treated with ADSC-CM and not. However, topical ADSC-CM treated wound revealed a better clinical improvement in epithelialization.

Download Full-text

Antler stem cell-conditioned medium stimulates regenerative wound healing in rats

Stem Cell Research & Therapy ◽

10.1186/s13287-019-1457-9 ◽

2019 ◽

Vol 10 (1) ◽

Cited By ~ 6

Author(s):

Xiaoli Rong ◽

Wenhui Chu ◽

Haiying Zhang ◽

Yusu Wang ◽

Xiaoyan Qi ◽

...

Keyword(s):

Wound Healing ◽

Stem Cell ◽

Conditioned Medium ◽

Dermal Fibroblasts ◽

Cutaneous Wound Healing ◽

Cutaneous Wound ◽

Nih 3T3 ◽

Cell Conditioned Medium

Abstract Background When the deer antler is cast, it leaves a cutaneous wound that can achieve scarless healing due to the presence of antler stem cells (ASCs). This provides an opportunity to study regenerative wound healing. Methods In this study, we investigated the therapeutic effects and mechanism of antler stem cell-conditioned medium (ASC-CM) on cutaneous wound healing in rats. In vitro, we investigated the effects of the ASC-CM on proliferation of HUVEC and NIH-3T3 cell lines. In vivo, we evaluated the effects of ASC-CM on cutaneous wound healing using full-thickness skin punch-cut wounds in rats. Results The results showed that ASC-CM significantly stimulated proliferation of the HUVEC and NIH-3T3 cells in vitro. In vivo, completion of healing of the rat wounds treated with ASC-CM was on day 16 (± 3 days), 9 days (± 2 days) earlier than the control group (DMEM); the area of the wounds treated with ASC-CM was significantly smaller (p < 0.05) than the two control groups. Further molecular characterization showed that the ratios of Col3A1/Col1A2, TGF-β3/TGF-β1, MMP1/TIMP1, and MMP3/TIMP1 significantly increased (p < 0.01) in the healed tissue in the ASC-CM group. Conclusions In conclusion, ASC-CM effectively accelerated the wound closure rate and enhanced the quality of healing, which might be through transforming wound dermal fibroblasts into the fetal counterparts. Therefore, the ASC-CM may have potential to be developed as a novel cell-free therapeutic for scarless wound healing.

Download Full-text